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Revisiones-Ciencias Básicas-Molecular, Bioquímica, Metabolismo & Genética *** Reviews-Basic Sciences-Molecular, Biochemistry, Metabolism & Genetics

 

TRASPLANTE RENAL *** RENAL TRANSPLANTATION

(Conceptos / Keywords: Renal-Kidney transplantation; Kidney donation-procurement; etc).

 

Enero / January 2001 --- Marzo / March 2004

 

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Enlace / Link

 

[1]

TÍTULO / TITLE:  - Interleukin-2 receptor monoclonal antibodies in renal transplantation: meta-analysis of randomised trials.

REVISTA / JOURNAL:  - British Medical J (BMJ). Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://bmj.com/search.dtl 

      ●● Cita: British Medical J. (BMJ): <> 2003 Apr 12;326(7393):789.

      ●● Enlace al texto completo (gratuito o de pago) 1136/bmj.326.7393.789

AUTORES / AUTHORS:  - Adu D; Cockwell P; Ives NJ; Shaw J; Wheatley K

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Queen Elizabeth Hospital, Birmingham, B15 2TH. dwomoa.adu@uhb.nhs.uk

RESUMEN / SUMMARY:  - OBJECTIVE: To study the effect of interleukin-2 receptor monoclonal antibodies on acute rejection episodes, graft loss, deaths, and rate of infection and malignancy in patients with renal transplants. DESIGN: Meta-analysis of published data. DATA SOURCES: Medline, Embase, and Cochrane library for years 1996-2003 plus search of medical editors’ trial amnesty and contact with manufacturers of the antibodies. SELECTION OF STUDIES: Randomised controlled trials comparing interleukin-2 receptor antibodies with placebo or no additional treatment in patients with renal transplants receiving ciclosporin based immunosuppression. RESULTS: Eight randomised controlled trials involving 1871 patients met the selection criteria (although only 1858 patients were analysed). Interleukin-2 receptor antibodies significantly reduced the risk of acute rejection (odds ratio 0.51, 95% confidence interval 0.42 to 0.63). There were no significant differences in the rate of graft loss (0.78, 0.58 to 1.04), mortality (0.75, 0.46 to 1.23), overall incidence of infections (0.97, 0.77 to 1.24), incidence of cytomegalovirus infections (0.81, 0.62 to 1.04), or risk of malignancies at one year (0.82, 0.39 to 1.70). The different antibodies had a similar sized effect on acute rejection (test for heterogeneity P=0.7): anti-Tac (0.37, 0.16 to 0.89), BT563 (0.37, 0.1 to 1.38), basiliximab (0.56, 0.44 to 0.72), and daclizumab (0.46, 0.32 to 0.67). The reduction in acute rejections was similar for all ciclosporin based immunosuppression regimens (test for heterogeneity P=1.0). CONCLUSIONS: Adding interleukin-2 receptor antibodies to ciclosporin based immunosuppression reduces episodes of acute rejection at six months by 49%. There is no evidence of an increased risk of infective complications. Longer follow up studies are needed to confirm whether interleukin-2 receptor antibodies improve long term graft and patient survival.

 

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[2]

TÍTULO / TITLE:  - Interleukin 2 receptor antagonists for renal transplant recipients: a meta-analysis of randomized trials.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 27;77(2):166-76.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000109643.32659.C4

AUTORES / AUTHORS:  - Webster AC; Playford EG; Higgins G; Chapman JR; Craig JC

INSTITUCIÓN / INSTITUTION:  - Cochrane Renal Group, Centre for Kidney Research, Children’s Hospital at Westmead, Westmead, NSW, Australia.

RESUMEN / SUMMARY:  - BACKGROUND: Interleukin 2 receptor antagonists (IL-2Ra) are increasingly used to treat renal transplant recipients. This study aims to systematically identify and summarize the effects of using IL-2Ra as induction immunosuppression, as an addition to standard therapy, or as an alternative to other antibody therapy. METHODS: Databases, reference lists, and abstracts of conference proceedings were searched extensively to identify relevant randomized controlled trials in all languages. Data were synthesized using the random effects model. Results are expressed as relative risk (RR), with 95% confidence intervals (CI). RESULTS: A total of 117 reports from 38 trials involving 4,893 participants were included. When IL-2Ra were compared with placebo (17 trials; 2,786 patients), graft loss was not significantly different at 1 year (14 trials: RR 0.84; CI 0.64-1.10) or 3 years (4 trials: RR 1.08; CI 0.71-1.64). Acute rejection was significantly reduced at 6 months (12 trials: RR 0.66; CI 0.59-0.74) and at 1 year (10 trials: RR 0.67; CI 0.60-0.75). At 1 year, cytomegalovirus infection (7 trials: RR 0.82; CI 0.65-1.03) and malignancy (9 trials: RR 0.67; CI 0.33-1.36) were not significantly different. When IL-2Ra were compared with other antibody therapy, no significant differences in treatment effects were demonstrated, but IL-2Ra had significantly fewer side effects. CONCLUSIONS: Given a 40% risk of rejection, seven patients would need treatment with IL-2Ra in addition to standard therapy, to prevent one patient from undergoing rejection, with no definite improvement in graft or patient survival. There is no apparent difference between basiliximab and daclizumab.

 

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[3]

TÍTULO / TITLE:  - A meta-analysis from the Cochrane Library reviewing interleukin 2 receptor antagonists in renal transplantation.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 27;77(2):165.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000112919.54256.8D

AUTORES / AUTHORS:  - Morris PJ; Monaco AP

 

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[4]

TÍTULO / TITLE:  - Renal transplantation: can we reduce calcineurin inhibitor/stop steroids? Evidence based on protocol biopsy findings.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2003 Mar;14(3):755-66.

AUTORES / AUTHORS:  - Gotti E; Perico N; Perna A; Gaspari F; Cattaneo D; Caruso R; Ferrari S; Stucchi N; Marchetti G; Abbate M; Remuzzi G

INSTITUCIÓN / INSTITUTION:  - Department of Medicine and Transplantation, Ospedali Riuniti di Bergamo, Mario Negri Institute for Pharmacological Research, Italy.

RESUMEN / SUMMARY:  - How to combine antirejection drugs and which is the optimal dose of steroids and calcineurin inhibitors beyond the first year after kidney transplantation to maintain adequate immunosuppression without major side effects are far from clear. Kidney transplant patients on steroid, cyclosporine (CsA), and azathioprine were randomized to per-protocol biopsy (n = 30) or no-biopsy (n = 29) 1 to 2 yr posttransplant. Steroid or CsA were discontinued or reduced on the basis of biopsy to establish effects on drug-related complications, acute rejection, and graft function over 3 yr of follow-up. Serum creatinine, GFR (plasma clearance of iohexol), RPF (renal clearance of p-aminohippurate), CsA pharmacokinetics, and adverse events were monitored yearly. At the end, patients underwent a second biopsy. Per-protocol biopsy histology revealed no lesions (n = 5, steroid withdrawal), CsA nephropathy (n = 13, CsA discontinuation/reduction), or chronic rejection (n = 12, standard therapy). Reducing the drug regimen led to overall fewer side effects related to immunosuppression as compared with standard therapy or no-biopsy. Steroids were safely stopped with no acute rejection or graft loss. Complete CsA discontinuation was associated with acute rejection in the first four patients. Lowering CsA to low target CsA trough (30 to 70 ng/ml) never led to acute rejection or major renal function deterioration. Biopsy patients on conventional regimen had no acute rejection, one graft loss, no significant change in GFR, and significant RPF decline. No-biopsy controls: no acute rejection, one graft loss, significant decline of GFR and RPF. By serial biopsy analysis, severe lesions did not develop in patients with steroid discontinuation in contrast to patients on standard therapy over follow-up. CsA reduction did not adversely affect histology. Per-protocol biopsy more than 1 yr after kidney transplantation is a safe procedure to guide change of drug regimen and to lower the risk of major side effects.

 

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[5]

TÍTULO / TITLE:  - Regulatory T cells in kidney transplant recipients: active players but to what extent?

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2003 Jun;14(6):1706-8.

AUTORES / AUTHORS:  - Zhai Y; Kupiec-Weglinski JW  N. Ref:: 20

 

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[6]

TÍTULO / TITLE:  - Interleukin 2 receptor antagonists for kidney transplant recipients.

REVISTA / JOURNAL:  - Cochrane Database Syst Rev 2004;1:CD003897.

      ●● Enlace al texto completo (gratuito o de pago) 1002/14651858.CD003897.pub2

AUTORES / AUTHORS:  - Webster A; Playford E; Higgins G; Chapman J; Craig J

INSTITUCIÓN / INSTITUTION:  - Centre for Kidney Research, The Children’s Hospital at Westmead, Locked Bag 4001, Westmead, NSW, AUSTRALIA, 2145.

RESUMEN / SUMMARY:  - BACKGROUND: Interleukin 2 receptor antagonists (IL2Ra) are used as induction therapy for prophylaxis against acute rejection in kidney transplant recipients. Use of IL2Ra has increased steadily, with 38% of new kidney transplant recipients in the United States, and 23% in Australasia receiving IL2Ra in 2002. OBJECTIVES: This study aims to systematically identify and summarise the effects of using an IL2Ra, as an addition to standard therapy, or as an alternative to other antibody therapy. SEARCH STRATEGY: The Cochrane Renal Group’s specialised register (June 2003), the Cochrane Controlled Trials Register (in The Cochrane Library issue 3, 2002), MEDLINE (1966-November 2002) and EMBASE (1980-November 2002). Reference lists and abstracts of conference proceedings and scientific meetings were hand-searched from 1998-2003. Trial groups, authors of included reports and drug manufacturers were contacted. SELECTION CRITERIA: Randomised controlled trials (RCTs) in all languages comparing IL2Ra to placebo, no treatment, other IL2Ra or other antibody therapy. DATA COLLECTION AND ANALYSIS: Data was extracted and quality assessed independently by two reviewers, with differences resolved by discussion. Dichotomous outcomes are reported as relative risk (RR) with 95% confidence intervals (CI). MAIN RESULTS: One hundred and seventeen reports from 38 trials involving 4893 participants were included. Where IL2Ra were compared with placebo (17 trials; 2786 patients), graft loss was not significantly different at one (RR 0.83, 95% CI 0.66 to 1.04) or three years (RR 0.88, 95% CI 0.64 to 1.22). Acute rejection (AR) was significantly reduced at six months (RR 0.66, 95% CI 0.59 to 0.74) and at one year (RR 0.67, 95% CI 0.60 to 0.75). At one year, cytomegalovirus (CMV) infection (RR 0.82, 95% CI 0.65 to 1.03) and malignancy (RR 0.67, 95% CI 0.33 to 1.36) were not significantly different. Where IL2Ra were compared with other antibody therapy no significant differences in treatment effects were demonstrated, but adverse effects strongly favoured IL2Ra. REVIEWER’S CONCLUSIONS: Given a 40% risk of rejection, seven patients would need treatment with IL2Ra to prevent one patient having rejection, with no definite improvement in graft or patient survival. There is no apparent difference between basiliximab and daclizumab. IL2Ra are as effective as other antibody therapies and with significantly fewer side effects

 

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[7]

TÍTULO / TITLE:  - Potential role of major histocompatibility complex class II peptides in regulatory tolerance to vascularized grafts.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 15;77(1 Suppl):S35-7.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000106472.91343.8D

AUTORES / AUTHORS:  - LeGuern C

INSTITUCIÓN / INSTITUTION:  - Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA. leguern@helix.mgh.harvard.edu

RESUMEN / SUMMARY:  - The inactivation of persisting T lymphocytes reactive to self- and non-self-antigens is a major arm of operational immune tolerance in mammals. Silencing of such T cells proceeds mostly by means of suppression, a process that is mediated by regulatory T-cell subsets and especially by CD4(+)CD(25high) regulatory T cells (Treg). Although Treg activation and ensuing suppressive activity appear to be major histocompatibility complex class II dependent, the fine specificity of Treg T-cell receptors has not yet been elucidated. Recent data from the author’s laboratory on a class II gene therapy induction of tolerance to allogeneic kidney grafts suggest that class II peptides are involved as generic signals for Treg activation. A brief compilation of results that would support this hypothesis is discussed in the present article.  N. Ref:: 31

 

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[8]

TÍTULO / TITLE:  - Transcriptional regulation of inflammatory genes before transplantation: a role for hypoxia inducible factor-1alpha?

REVISTA / JOURNAL:  - Transplantation 2003 Feb 27;75(4):437-8.

AUTORES / AUTHORS:  - Koo DD; Fuggle SV

INSTITUCIÓN / INSTITUTION:  - Nuffield Department of Surgery, University of Oxford, Oxford Transplant Centre, United Kingdom.  N. Ref:: 5

 

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[9]

TÍTULO / TITLE:  - Complement and the kidney.

REVISTA / JOURNAL:  - J Immunol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jimmunol.org/ 

      ●● Cita: J. of Immunology: <> 2003 Oct 1;171(7):3319-24.

AUTORES / AUTHORS:  - Quigg RJ

INSTITUCIÓN / INSTITUTION:  - Section of Nephrology, University of Chicago, Chicago, IL 60637, USA. rqigg@medicine.uchicago.edu  N. Ref:: 94

 

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[10]

TÍTULO / TITLE:  - The economic value of valacyclovir prophylaxis in transplantation.

REVISTA / JOURNAL:  - J Infect Dis. Acceso gratuito al texto completo a partir de los 2 años de la publicación;  - http://www.journals.uchicago.edu/ 

      ●● Cita: J. of Infectious Diseases: <> 2002 Oct 15;186 Suppl 1:S116-22.

AUTORES / AUTHORS:  - Squifflet JP; Legendre C

INSTITUCIÓN / INSTITUTION:  - University Clinic Saint Luc, 1200 Brussels, Belgium. Jean-Paul.Squifflet@chir.ucl.ac.be

RESUMEN / SUMMARY:  - Cytomegalovirus (CMV) infection and disease, with its extensive direct and indirect consequences, adds considerably to the cost of patient management in both solid organ and bone marrow transplantation. Antiviral prophylaxis for CMV infection can offer cost advantages over preemptive therapy and “wait-and-treat” approaches. Valacyclovir has demonstrated efficacy for CMV prophylaxis in renal, heart, and bone marrow transplantation and is cost-effective when compared with placebo in renal transplant recipients at high risk of CMV infection. In reducing CMV infection and disease, valacyclovir prophylaxis appears to be associated with reductions in indirect effects of CMV (acute graft rejection, other opportunistic infections) and, if these effects are considered, the potential exists for even greater savings to be made with valacyclovir therapy. Benefits of valacyclovir in transplantation extend beyond CMV to other herpesviruses and may be increased in some clinical situations by prolonging prophylaxis beyond 3 months.  N. Ref:: 32

 

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[11]

- Castellano -

TÍTULO / TITLE:Polimorfismo del receptor de la vitamina D y enfermedad osea postrasplante renal. Polymorphism of the vitamin D receptor and bone disease after renal transplantation.

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2001;21 Suppl 1:56-60.

AUTORES / AUTHORS:  - Torres A; Barrios Y; Salido E

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia y, Hospital Universitario de Canarias, Instituto Reina Sofia de Investigacion Nefrologica, Tenerife, España. atorres@ull.es  N. Ref:: 29

 

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[12]

- Castellano -

TÍTULO / TITLE:La influencia de los factores geneticos en la patogenesis de dislipidemias posteriores al trasplante renal. The effect of genetic factors on the pathogenesis of the dyslipidemias following kidney transplantation.

REVISTA / JOURNAL:  - Rev Invest Clin. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.imbiomed.com/ 

      ●● Cita: Revista de Investigacion Clinica: <> 2002 Sep-Oct;54(5):472-3.

AUTORES / AUTHORS:  - Lerman Garber I

INSTITUCIÓN / INSTITUTION:  - Departamento de Endocrinologia y Metabolismo, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran. lerman@netservice.com.mx  N. Ref:: 11

 

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[13]

TÍTULO / TITLE:  - A benefit-risk assessment of basiliximab in renal transplantation.

REVISTA / JOURNAL:  - Drug Saf. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.csmwm.org/ 

      ●● Cita: Drug Safety: <> 2004;27(2):91-106.

AUTORES / AUTHORS:  - Boggi U; Danesi R; Vistoli F; Del Chiaro M; Signori S; Marchetti P; Del Tacca M; Mosca F

INSTITUCIÓN / INSTITUTION:  - Division of General Surgery and Transplants, Department of Oncology, Transplants and Advanced Technologies in Medicine, University of Pisa, Pisa, Italy. uboggi@med.unipi.it

RESUMEN / SUMMARY:  - Interleukin-2 (IL-2) and its receptor (IL-2R) play a central role in T lymphocyte activation and immune response after transplantation. Research on the biology of IL-2R allowed the identification of key signal transduction pathways involved in the generation of proliferative and antiapoptotic signals in T cells. The alpha-chain of the IL-2R is a specific peptide against which monoclonal antibodies have been raised, with the aim of blunting the immune response by means of inhibiting proliferation and inducing apoptosis in primed lymphocytes. Indeed, basiliximab, one of such antibodies, has proved to be effective in reducing the episodes of acute rejection after kidney and pancreas transplantation. The use of basiliximab was associated with a significant reduction in the incidence of any treated rejection episodes after kidney transplantation in the two major randomised studies (placebo 52.2% vs basiliximab 34.2% at 6 months, European study; placebo 54.9% vs basiliximab 37.6% at 1 year, US trial). Basiliximab and equine antithymocyte globulin (ATG) administration resulted in a similar rate of biopsy-proven acute rejection at 6 months (19% for both) and at 12 months (19% and 20%, respectively). The use of basiliximab appears not to be associated with an increased incidence of adverse events as compared with placebo in immunosuppressive regimens, including calcineurin inhibitors, mycophenolate mofetil or azathioprine and corticosteroids, and its safety profile is superior to ATG. Moreover, a similar occurrence of infections is noted in selected studies (65.5% after basiliximab vs 65.7% of controls), including cytomegalovirus infection (17.3% vs 14.5%), and cytokine-release syndrome is not observed. Finally, economic analysis demonstrated lower costs of overall treatment in patients treated with basiliximab. Therefore, the use of basiliximab entails a very low risk, allows safe reduction of corticosteroid dosage and reduces the short- and mid-term rejection rates. However, the improvement in the long-term survival of kidney grafts in patients treated according to modern immunosuppressive protocols is still to be demonstrated. These conclusions are based on a systematic review of the scientific literature, indexed on Medline database, concerning the mechanism of action, therapeutic activity, safety and pharmacoeconomic evaluation of basiliximab in renal transplantation.  N. Ref:: 62

 

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[14]

TÍTULO / TITLE:  - Renal transplantation in HBsAg+ patients: is lamivudine your “final answer”?

REVISTA / JOURNAL:  - J Clin Gastroenterol 2003 Jul;37(1):9-11.

AUTORES / AUTHORS:  - Fontana RJ  N. Ref:: 30

 

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[15]

TÍTULO / TITLE:  - Current treatment strategies in ANCA-positive renal vasculitis-lessons from European randomized trials.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2003 Jul;18 Suppl 5:v2-4.

AUTORES / AUTHORS:  - Tesar V; Rihova Z; Jancova E; Rysava R; Merta M

INSTITUCIÓN / INSTITUTION:  - First Medical Department, First Medical Faculty, Charles University, Prague, Czech Republic. tesar@beba.cesnet.cz

RESUMEN / SUMMARY:  - Antineutrophil cytoplasmic antibody (ANCA)-positive renal vasculitis is the most common cause of rapidly progressive (crescentic) glomerulonephritis. Its life-threatening natural course may be modified substantially by current treatment modalities. The European Vasculitis Study Group (EUVAS) developed a subclassification of ANCA-positive vasculitides based on the disease severity at presentation, and have organized (so far) two waves of clinical trials. The first wave of randomized clinical trials had the aim of optimizing the existing therapeutic regimens; the second wave concentrated on testing some newer therapeutic approaches. Here, the design and available results of the first wave and the design of some second wave trials are reviewed briefly. The potential of the new targeted approaches (e.g. anti-tumour necrosis factor therapy) is also briefly mentioned.  N. Ref:: 9

 

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[16]

TÍTULO / TITLE:  - Angiotensin II type 1 (AT1) receptor antagonists in the treatment of hypertension after renal transplantation.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2001;16 Suppl 1:117-20.

AUTORES / AUTHORS:  - Holgado R; Anaya F; Del Castillo D

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia, Hospital Reina Sofia, 14012 Cordoba, España.

RESUMEN / SUMMARY:  - Hypertension is highly prevalent after renal transplantation and has been associated with lower graft survival. Optimum management of post-transplant hypertension remains to be defined. Losartan, a potent, orally active and selective non-peptide blocker of the angiotensin subtype 1 receptor, could represent a useful drug for treating post-transplant hypertension. Recently, a prospective study of 12 weeks treatment with losartan has showed a satisfactory control of arterial hypertension associated with a decrease in proteinuria in this high-risk group of renal transplant patients. A retrospective study was performed to review the role of losartan as a renoprotective agent (evaluating blood pressure and proteinuria) in renal transplant recipients in a long-term follow-up. A total of 150 transplant recipients were included in the study. None of the patients had a serum creatinine >3 mg/dl, or suspected renal artery stenosis, or other severe concomitant diseases. The indication for losartan therapy was hypertension, proteinuria and/or post-transplant erythrocytosis. The values of blood pressure, results of fasting haematology, blood chemistry and total proteinuria in 24-h urine samples were recorded at the time of initiation of losartan therapy, 6 and 3 months before the start, and at 3, 6, 12, 18 and 24 months thereafter. A tendency analysis by linear regression comparing two slopes before and after treatment was realized. A decrease in mean blood pressure and proteinuria, from 106.7+/-0.9 to 98.2+/-2.1 mmHg and from 1253.9+/-188 to 91.2+/-33.7 mg/24 h, P<0.05, respectively, was observed after introduction of losartan. A progressive increase in creatinine clearance was observed after the third month of losartan treatment. No significant changes were seen in haematocrit or serum potassium levels. We can conclude that a progressive decrease in mean arterial pressure associated with a decrease in proteinuria was observed during long-term follow-up. Based on the capacity of losartan to improve renal function, this drug could be decisive for the treatment and prevention of chronic allograft nephropathy.  N. Ref:: 32

 

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[17]

TÍTULO / TITLE:  - Calcineurin-free protocols with basiliximab induction allow patients included in “old to old” programs achieve standard kidney transplant function.

REVISTA / JOURNAL:  - Transplant Proc 2003 Jun;35(4):1326-7.

AUTORES / AUTHORS:  - Emparan C; Laukotter M; Wolters H; Dame C; Heidenreich S; Senninger N

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Division of Transplantation, Uniklinikum Munster, Munster, Germany. cemparan@teleline.es

RESUMEN / SUMMARY:  - INTRODUCTION: The EuroTransplant “old to old” program establishes that patients older than 60 years can receive offers of organs from donors older than 60 years. The compromised function of these organs makes it a priority to preserve their initial kidney function. HYPOTHESIS: Calcineurin-sparing protocols using anti-IL-2 receptor (IL-2R) antibody induction (Simulect) may benefit initial kidney function in these patients, as assessed by the rates of delayed graft function and of rejection during the first month after transplant. PATIENTS AND METHODS: A cohort of 15 consecutive elderly patients were prospectively compared with 30 cadaveric kidney transplants in younger recipients. Study patients were induced with Simulect (20 mg, 30 minutes before reperfusion and 4 days after transplantation) and steroids, delaying the introduction of CsA until the serum creatinine was below 3 mg/dL. The other cohort of patients were immunosuppressed with tacrolimus (trough 8 to 12), mycophenolats mofetil (MMF, 1 g/d), and an identical taper of steroids. The analysis compared donor and recipient ages, mean cold ischemic time, incidence of initial kidney function (diuresis in the first 24 h) serum creatinine levels, glomerular filtration rate (GFR), number of dialysis sessions, and rejection rate in the two groups. RESULTS: Except for the donor and recipient ages (72 vs 54 in donors, and 67 versus 52 years in recipients), no significant differences were observed between the groups among the rates of acute rejection (6.6% vs 13.2%), delayed graft function (13.2% required dialysis), or infection (6.6%). Within 1 month all 45 grafts showed primary function with equal creatinine levels (mean 1.65). CONCLUSIONS: Calcineurin-free protocols using IL-2 therapy as the initial suppression allow patients in the “old to old” ET program to display equal results to cadaveric kidney transplants with initial treatment with calcineurin antagonists.

 

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[18]

TÍTULO / TITLE:  - ACE inhibitors and AII receptor antagonists in the treatment and prevention of bone marrow transplant nephropathy.

REVISTA / JOURNAL:  - Curr Pharm Des 2003;9(9):737-49.

AUTORES / AUTHORS:  - Moulder JE; Fish BL; Cohen EP

INSTITUCIÓN / INSTITUTION:  - Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. jmoulder@its.mcw.edu

RESUMEN / SUMMARY:  - Radiation nephropathy has emerged as a major complication of bone marrow transplantation (BMT) when total body irradiation (TBI) is used as part of the regimen. Classically, radiation nephropathy has been assumed to be inevitable, progressive, and untreatable. However, in the early 1990’s, it was demonstrated that experimental radiation nephropathy could be treated with a thiol-containing ACE inhibitor, captopril. Further studies showed that enalapril (a non-thiol ACE inhibitor) was also effective in the treatment of experimental radiation nephropathy, as was an AII receptor antagonist. Studies also showed that ACE inhibitors and AII receptor antagonists were effective in the prophylaxis of radiation nephropathy. Interestingly, other types of antihypertensive drugs were ineffective in prophylaxis, but brief use of a high-salt diet in the immediate post-irradiation period decreased renal injury. A placebo-controlled trial of captopril to prevent BMT nephropathy in adults is now underway. Since excess activity of the renin-angiotensin system (RAS) causes hypertension, and hypertension is a major feature of radiation nephropathy; an explanation for the efficacy of RAS antagonism in the prophylaxis of radiation nephropathy would be that radiation leads to RAS activation. However, current studies favor an alternative explanation, namely that the normal activity of the RAS is deleterious in the presence of radiation injury. On-going studies suggest that efficacy of RAS antagonists may involve interactions with a radiation-induced decrease in renal nitric oxide activity or with radiation-induced tubular cell proliferation. We hypothesize that while prevention (prophylaxis) of radiation nephropathy with ACE inhibitors, AII receptor antagonists, or a high-salt diet work by suppression of the RAS, the efficacy of ACE inhibitors and AII receptor antagonists in treatment of established radiation nephropathy depends on blood pressure control.  N. Ref:: 108

 

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[19]

TÍTULO / TITLE:  - European best practice guidelines for renal transplantation. Section IV: Long-term management of the transplant recipient. IV.3.1 Long-term immunosuppression. Late steroid or cyclosporine withdrawal.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2002;17 Suppl 4:19-20.

RESUMEN / SUMMARY:  - GUIDELINES: A. In order to reduce or avoid long-term serious adverse effects of corticosteroids, such as bone fractures, diabetes mellitus, arterial hypertension, osteoporosis and eye complications, steroid withdrawal should be considered. B. Steroid withdrawal is safe only in a proportion of graft recipients and is recommended only in low-risk patients. The efficacy of the remaining immunosuppression should be considered. C. After steroid withdrawal, graft function has to be monitored very carefully because of the risk of a delayed but continuous loss of function due to chronic graft dysfunction. In the case of functional deterioration or dysfunction, steroids should be re-administered. D. Cyclosporine withdrawal might be considered in order to ameliorate nephrotoxicity, arterial hypertension, lipid disorders and hypertrichosis. This can be carried out with no significant long-term risk of progressive graft loss. The efficacy of the remaining immunosuppression should be considered. After cyclosporine withdrawal, careful monitoring for acute rejection is recommended.

 

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[20]

TÍTULO / TITLE:  - Angiopoietin growth factors and Tie receptor tyrosine kinases in renal vascular development.

REVISTA / JOURNAL:  - Pediatr Nephrol 2001 Feb;16(2):177-84.

AUTORES / AUTHORS:  - Woolf AS; Yuan HT

INSTITUCIÓN / INSTITUTION:  - Nephro-Urology Unit, Institute of Child Health, University College London, UK. a.woolf@ich.ucl.ac.uk

RESUMEN / SUMMARY:  - Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilises capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms’ kidney tumours and in vascular remodelling after nephrotoxicity.  N. Ref:: 64

 

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[21]

TÍTULO / TITLE:  - Early prognosis of the development of renal chronic allograft rejection by gene expression profiling of human protocol biopsies.

REVISTA / JOURNAL:  - Transplantation 2003 Apr 27;75(8):1323-30.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000068481.98801.10

AUTORES / AUTHORS:  - Scherer A; Krause A; Walker JR; Korn A; Niese D; Raulf F

INSTITUCIÓN / INSTITUTION:  - Novartis Institutes for BioMedical Research/Transplantation, Novartis Pharma AG, Basel, Switzerland.

RESUMEN / SUMMARY:  - BACKGROUND: Chronic allograft rejection (CR) is the major cause of failure of long-term graft survival and is so far irreversible. Early prognosis of CR by molecular markers before overt histologic manifestation would be a valuable aid for the optimization of treatment regimens and the design of clinical CR trials. Oligonucleotide microarray-based approaches have proven to be useful for the diagnosis and prognosis of a variety of diseases and were chosen for the unbiased identification of prognostic biomarkers. METHODS: Renal allograft biopsies were taken at month 6 posttransplantation (PT) from two groups who were, at that time, healthy recipients: one group developed CR at month-12 PT, the other group remained healthy. Gene expression profiles from the two groups at month-6 PT biopsies were analyzed to identify differentially expressed genes with prognostic value for CR development at month 12. RESULTS: A set of 10 genes was identified that showed differential expression profiles between the two patient groups and had a complete separation of the 15% to 85% quantile range for each individual gene. This set of genes was sufficient to allow the correct prediction of the occurrence or nonoccurrence of CR in 15 of 17 (88%) patients using cross-validation (occurrence for a patient was predicted on the basis of the other patients’ data only). In addition, a correct prediction could be made that a recipient with a normal biopsy 12 months PT developed CR within the following 6 months. CONCLUSIONS: Identified expression patterns seem to be highly prognostic of the development of renal CR.

 

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[22]

TÍTULO / TITLE:  - Calcium metabolism and skeletal problems after transplantation.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2002 Feb;13(2):551-8.

AUTORES / AUTHORS:  - Torres A; Lorenzo V; Salido E

INSTITUCIÓN / INSTITUTION:  - Nephrology Section and Research Unit, University Hospital of the Canary Islands, Instituto Reina Sofia de Investigacion, Tenerife, España. atorres@ull.es  N. Ref:: 59

 

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[23]

TÍTULO / TITLE:  - The impact of impaired insulin release and insulin resistance on glucose intolerance after renal transplantation.

REVISTA / JOURNAL:  - Clin Transplant 2002 Dec;16(6):389-96.

AUTORES / AUTHORS:  - Hjelmesaeth J; Hagen M; Hartmann A; Midtvedt K; Egeland T; Jenssen T

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, Section of Nephrology, Oslo, Norway. joran@online.no

RESUMEN / SUMMARY:  - The current knowledge of the pathogenesis of post-transplant glucose intolerance is sparse. This study was undertaken to assess the relative importance of insulin secretion (ISec) and insulin sensitivity (IS) in the pathogenesis of post-transplant diabetes mellitus (PTDM), impaired glucose tolerance (IGT) and impaired fasting glucose (IFG) after renal transplantation. An oral glucose tolerance test (OGTT) was performed in 167 non-diabetic recipients 10 wk after renal transplantation. Fasting, 1-h and 2-h insulin and glucose levels were used to estimate the insulin secretory response and IS. One year after transplantation 89 patients were re-examined with an OGTT including measurements of fasting and 2 h glucose. Ten weeks after transplantation the PTDM-patients had significantly lower ISec and IS than patients with IGT/IFG, who again had lower ISec and IS than those with normal glucose tolerance (NGT). One year later, a similar difference in baseline ISec was observed between the three groups, whereas baseline IS did not differ significantly. Patients who improved their glucose tolerance during the first year, were mainly characterized by a significantly greater baseline ISec, and they received a significantly higher median prednisolone dose at baseline with a subsequent larger dose reduction during the first year, than the patients who had their glucose tolerance unchanged or worsened. In conclusion, both impaired ISec and IS characterize patients with PTDM and IGT/IFG in the early course after renal transplantation. The presence of defects in insulin release, rather than insulin action, indicates a poor prognosis regarding later normalization of glucose tolerance.  N. Ref:: 29

 

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[24]

TÍTULO / TITLE:  - Basiliximab: a review of its use as induction therapy in renal transplantation.

REVISTA / JOURNAL:  - Drugs 2003;63(24):2803-35.

AUTORES / AUTHORS:  - Chapman TM; Keating GM

INSTITUCIÓN / INSTITUTION:  - Adis International Limited, Auckland, New Zealand. demail@adis.co.nz

RESUMEN / SUMMARY:  - Basiliximab (Simulect), a chimeric (human/murine) monoclonal antibody, is indicated for the prevention of acute organ rejection in adult and paediatric renal transplant recipients in combination with other immunosuppressive agents.Basiliximab significantly reduced acute rejection compared with placebo in renal transplant recipients receiving dual- (cyclosporin microemulsion and corticosteroids) or triple-immunotherapy (azathioprine- or mycophenolate mofetil-based); graft and patient survival rates at 12 months were similar. Significantly more basiliximab than placebo recipients were free from the combined endpoint of death, graft loss or acute rejection 3 years, but not 5 years, after transplantation.The incidence of adverse events was similar in basiliximab and placebo recipients, with no increase in the incidence of infection, including cytomegalovirus (CMV) infection. Malignancies or post-transplant lymphoproliferative disorders after treatment with basiliximab were rare, with a similar incidence to that seen with placebo at 12 months or 5 years post-transplantation. Rare cases of hypersensitivity reactions to basiliximab have been reported.The efficacy of basiliximab was similar to that of equine antithymocyte globulin (ATG) and daclizumab, and similar to or greater than that of muromonab CD3. Basiliximab was as effective as rabbit antithymocyte globulin (RATG) in patients at relatively low risk of acute rejection, but less effective in high-risk patients. Numerically or significantly fewer patients receiving basiliximab experienced adverse events considered to be related to the study drug than ATG or RATG recipients. The incidence of infection, including CMV infection, was similar with basiliximab and ATG or RATG.Basiliximab plus baseline immunosuppression resulted in no significant differences in acute rejection rates compared with baseline immunosuppression with or without ATG or antilymphocyte globulin in retrospective analyses conducted for small numbers of paediatric patients. Limited data from paediatric renal transplant recipients suggest a similar tolerability profile to that in adults. Basiliximab appears to allow the withdrawal of corticosteroids or the use of corticosteroid-free or calcineurin inhibitor-sparing regimens in renal transplant recipients.Basiliximab did not increase the overall costs of therapy in pharmacoeconomic studies.CONCLUSION: Basiliximab reduces acute rejection without increasing the incidence of adverse events, including infection and malignancy, in renal transplant recipients when combined with standard dual- or triple-immunotherapy. The overall incidence of death, graft loss or acute rejection was significantly reduced at 3 years; there was no significant difference for this endpoint 5 years after transplantation. Malignancy was not increased at 5 years. The overall efficacy, tolerability, ease of administration and cost effectiveness of basiliximab make it an attractive option for the prophylaxis of acute renal transplant rejection.  N. Ref:: 85

 

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[25]

TÍTULO / TITLE:  - Pharmacokinetics of tacrolimus-based combination therapies.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2003 May;18 Suppl 1:i12-5.

AUTORES / AUTHORS:  - Undre NA

INSTITUCIÓN / INSTITUTION:  - Fujisawa GmbH, Neumarkter Str. 61, D-81673 Munich, Germany. nas.undre@fujisawa.de

RESUMEN / SUMMARY:  - This paper reviews the pharmacokinetics of tacrolimus, with special reference to its combination with adjunctive immunosuppressants. Oral bioavailability of tacrolimus, which is variable between patients, averages approximately 25%. This is largely due to extrahepatic metabolism of tacrolimus in the gastrointestinal epithelium. Nevertheless, intra-patient variability is low, as evidenced by the small number of dose changes required to maintain patients within the recommended tacrolimus target levels. Tacrolimus is distributed extensively in the body with most partitioned outside the blood compartment. Concentrations of tacrolimus in blood are used as a surrogate marker of clinically relevant concentration of the drug at the site(s) of action. Convenient whole-blood sampling within a +/-2-h window around 12 h post-dose (C(min)) is highly predictive of systemic exposure to tacrolimus and is thus used to optimise therapy. Sampling at other time-points offers no advantage over C(min) monitoring. The interactions of tacrolimus with other immunosuppressive agents are well characterized. After cessation of concomitant corticosteroid treatment, exposure to tacrolimus increases by approximately 25%. In contrast, there is no pharmacokinetic interaction between mycophenolate mofetil (MMF) and tacrolimus. Therefore, systemic exposure to the active metabolite of MMF, mycophenolic acid, is higher with MMF-tacrolimus combination than with MMF-cyclosporin combination. Therefore, 1 g/day MMF may be an adequate maintenance dose in tacrolimus-based regimens. Co-administration of tacrolimus and sirolimus, while having no effect on exposure to sirolimus, results in reduced exposure to tacrolimus at sirolimus doses of 2 mg/day and above. In conclusion, tacrolimus levels should be monitored when sirolimus is co-administered at doses >2 mg/day and after cessation of corticosteroid treatment.  N. Ref:: 13

 

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[26]

- Castellano -

TÍTULO / TITLE:La enfermedad linfoproliferativa difusa postrasplante renal y su relacion con el virus Epstein-Barr. Experiencia de un centro. Diffuse lymphoproliferative disease after renal transplantation and its relation with Epstein-Barr virus. Experience at one center.

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2002;22(5):463-9.

AUTORES / AUTHORS:  - Franco A; Jimenez L; Aranda I; Alvarez L; Gonzalez M; Rocamora N; Olivares J

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia Hospital General Alicante Maestro Alonso, 109 03010 Alicante. franco_ant@gva.es

RESUMEN / SUMMARY:  - Post-transplant lymphoproliferative disorders (PTLD) are a group of heterogeneous lymphoid proliferations in chronic immunosuppressed recipients which appear to be related to Epstein Barr Virus (EBV). Receptor EBV seronegativity, use of antilymphocyte antibodies and CMV disease have been identified as risk factors that may tigger development of PTLD. We have studied the incidence of PTLD and its relationship with EBV in 588 adult renal transplant recipients who were transplanted in our hospital from 1988 to 2001. We have also evaluated the diagnostic and therapeutic methods used, the risk factors and outcome of the patients who developed PTLD. We identified 8 recipients (4 males and 4 females), range from 18 to 67 years (mean age 45.6 years) with a median time between grafting and PTLD of 4.1 years (0.1-7 years), who developed PTLD (1.3%). Only 1 patient received OKT3 and had CMV disease, two of them (25%) had been treated with hight doses of prednisolone, another was EBV seronegative, but the rest of them (50%) had no risk factors. Two patients were diagnosed at autopsy, the diagnosis of 5 was based on the histology of biopsy and the last one by CT scans of chest-abdomen and cytology. The presence of EBV in the lymphoproliferative cells was assessed in 5 out of the 7 studied patients (71.4%). The outcome of our recipients was poor. Five out of 8 patients died shortly after diagnosis as a direct consecuence of PTLD and another of an infectious complication of the treatment (75%). The 2 patients alive started dialysis and 1 of them died 2 years later of a non-related cause. In conclusion, PTLD is a relatively frequent disease with a poor prognosis in renal transplant patients. It seems to have a close relationship with EBV and can develop in the absence of the classical risk factors.  N. Ref:: 18

 

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[27]

TÍTULO / TITLE:  - Capillary C4d deposition as a marker of humoral immunity in renal allograft rejection.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2002 Sep;13(9):2420-3.

AUTORES / AUTHORS:  - Watschinger B; Pascual M  N. Ref:: 38

 

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[28]

TÍTULO / TITLE:  - TGF-beta(1) gene expression in protocol biopsies from patients with stable renal allograft function.

REVISTA / JOURNAL:  - Transplant Proc 2001 Feb-Mar;33(1-2):342-4.

AUTORES / AUTHORS:  - Hueso M; Bover J; Espinosa L; Moreso F; Seron D; Canas C; Raulf F; Blanco A; Gil-Vernet S; Carreras M; Castelao AM; Grinyo JM; Alsina J

INSTITUCIÓN / INSTITUTION:  - Nephrology Department, CSUB, L’Hospitalet de Llobregat, Barcelona, España.

 

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[29]

- Castellano -

TÍTULO / TITLE:Alteraciones del metabolismo oseo tras el trasplante renal. Bone metabolism alterations after kidney transplantation.

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2003;23 Suppl 2:122-6.

AUTORES / AUTHORS:  - Torres A; Garcia S; Barrios Y; Hernandez D; Lorenzo V

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia, Unidad de Investigacion, Hospital Universitario de Canarias, Instituto Reina Sofia de Investigacion. atorres@ull.es

RESUMEN / SUMMARY:  - Early after renal transplantation (RT) a rapid decrease in bone mineral density at the lumbar spine, femoral neck, and femoral shaft has been documented. In addition, an appreciable proportion of patients still remain losing bone late after RT. As a consequence, RT patients are at a high risk of bone fractures as compared to general population. Most fractures involve appendicular skeleton, particularly the feet and ankles, and the diabetic patient is at increased risk of fractures. Thus, early institution of preventive measures and treatment of established osteoporosis are central. The major cause of post-transplantation bone loss is corticosteroid treatment, and this should be used at the lower dose compatible with graft survival. Preexisting hyperparathyroidism also affects the early cancellous bone loss at the spine, and post-transplantation bone loss reflects variable individual susceptibility, resembling the polygenic determination of bone mineral density in general. Clinical trials have demonstrated that bisphosphonates or vitamin D plus calcium supplementation, prevent post-transplantation bone loss during the first 6-12 months. However, their role in preventing bone fractures has not been proven. Finally, recommendations for management, prevention and treatment, are summarized.  N. Ref:: 24

 

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[30]

TÍTULO / TITLE:  - Efficacy and toxicity of a protocol using sirolimus, tacrolimus and daclizumab in a nonhuman primate renal allotransplant model.

REVISTA / JOURNAL:  - Am J Transplant 2002 Apr;2(4):381-5.

AUTORES / AUTHORS:  - Montgomery SP; Mog SR; Xu H; Tadaki DK; Hirshberg B; Berning JD; Leconte J; Harlan DM; Hale D; Kirk AD

INSTITUCIÓN / INSTITUTION:  - NIDDK/Navy Transplantation and Autoimmunity Branch, Naval Medical Research Center, Bethesda, Maryland 20892, USA.

RESUMEN / SUMMARY:  - A regimen combining sirolimus, tacrolimus, and daclizumab has recently been shown to provide adequate immunosuppression for allogeneic islet transplantation in humans, but remains unproven for primarily vascularized allografts. We evaluated this regimen for renal allograft transplantation in mismatched nonhuman primates. Dosages of sirolimus and tacrolimus were adjusted for trough levels of 10-15 ng/mL and 4-6 ng/mL, respectively. Treated monkeys (n = 5) had significantly prolonged allograft survival, with a mean survival of 36 days vs. 7 days in untreated controls (n = 6, p = 0.008). Four of five treated animals, but none of the controls, developed fibrinoid vascular necrosis of the small intestine. A review of gut histology from animals on other immunosuppressive protocols performed by our laboratory suggested that these lesions were a result of sirolimus exposure. In summary, this regimen prolongs the survival of vascularized renal allografts, but is limited by profound GI toxicity in rhesus macaques.

 

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[31]

TÍTULO / TITLE:  - Interleukin 18 and interleukin 18 binding protein: possible role in immunosuppression of chronic renal failure.

REVISTA / JOURNAL:  - Blood Purif 2003;21(3):258-70.

      ●● Enlace al texto completo (gratuito o de pago) 1159/000070699

AUTORES / AUTHORS:  - Dinarello CA; Novick D; Rubinstein M; Lonnemann G

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, University of Colorado Health Sciences Center, Denver, Colo 80262, USA.

RESUMEN / SUMMARY:  - Although interleukin (IL)-18 is a member of the IL-1 family of ligands, IL-18 appears to have unique characteristics, particularly in the regulation of the T helper type 1 (Th1) response. Th1 responses are required for tumor surveillance, killing intracellular organisms, and to provide help for antibody production. In patients with chronic renal failure, the well-known immunosuppression contributes to a failure to respond to infectious challenges and vaccinations. The most salient biological property of IL-18, linking this cytokine to the Th1 response, is its ability to induce interferon gamma (IFN-gamma). In fact, IL-18 was originally identified as an IFN-gamma-inducing factor, and IFN-gamma production is the hallmark of the Th1 response. Dysregulation of IFN-gamma production resulting from reduced activity of IL-18 would explain one of the mechanisms of immunosuppression in patients with chronic renal failure. The activity of IL-18 can be regulated by the IL-18-binding protein (IL-18BP), a glycoprotein of 40,000 daltons, which is constitutively expressed and appears to be the natural inhibitor of IL-18 activity. Unlike soluble receptors for IL-18, IL-18BP does not have a transmembrane domain; IL-18BP is a secreted protein possessing a high-affinity binding and ability to neutralize IL-18. IL-18BP was discovered in human urine and is excreted in health following glomerular filtration. With decreasing renal function, the concentrations of IL-18BP in the circulation are elevated as compared with subjects with a normal renal function, and these elevated levels may result in a decreased IL-18 activity. Because of the importance of IL-18 and IFN-gamma in the Th1 response, the biology of IL-18 and IL-18BP is reviewed here in the context of the immunosuppression of chronic renal failure.  N. Ref:: 81

 

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[32]

TÍTULO / TITLE:  - C4d and the fate of organ allografts.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2002 Sep;13(9):2417-9.

AUTORES / AUTHORS:  - Platt JL  N. Ref:: 16

 

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[33]

TÍTULO / TITLE:  - The evolving role of chemokines and their receptors in acute allograft rejection.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2002 Aug;17(8):1374-9.

AUTORES / AUTHORS:  - Inston NG; Cockwell P

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology and Renal Transplantation, Queen Elizabeth Hospital, University Hospital Birmingham NHS Trust, Birmingham, UK.  N. Ref:: 64

 

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[34]

TÍTULO / TITLE:  - Renal transplantation studies in genetic hypertension.

REVISTA / JOURNAL:  - News Physiol Sci. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://nips.physiology.org/contents-by-date.0.shtml 

      ●● Cita: News in Physiological Sciences: <> 2001 Dec;16:262-5.

AUTORES / AUTHORS:  - Grisk O; Rettig R

INSTITUCIÓN / INSTITUTION:  - Department of Physiology, University of Greifswald, D-17487 Greifswald, Germany.  N. Ref:: 17

 

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[35]

- Castellano -

TÍTULO / TITLE:Aplicaciones de la biologia molecular en el trasplante renal. Applications of molecular biology to renal transplant.

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2003;23(1):15-26.

AUTORES / AUTHORS:  - Lario S; Bescos M; Campistol JM

INSTITUCIÓN / INSTITUTION:  - Unidad de Trasplante Renal, Hospital Clinic, de Barcelona Villarroel, 170 08036 Barcelona. jmcampis@medicina.ub.es  N. Ref:: 35

 

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[36]

TÍTULO / TITLE:  - General health management and long-term care of the renal transplant recipient.

REVISTA / JOURNAL:  - Am J Kidney Dis 2001 Dec;38(6 Suppl 6):S10-24.

AUTORES / AUTHORS:  - Cohen D; Galbraith C

INSTITUCIÓN / INSTITUTION:  - Columbia Presbyterian Hospital, New York, NY 10032, USA. djc5@columbia.edu

RESUMEN / SUMMARY:  - The steady improvement in short-term success rates in renal transplant patients has translated into better long-term success rates and a large number of patients with long-functioning renal transplants. The necessity for the lifelong administration of immunosuppressive medications to prevent rejection, coupled with the presence in many patients of a variety of other medical problems dating from the period of renal insufficiency prior to the time of renal transplantation, has created a large group of patients with a unique and complex set of long-term medical care needs. Due to the constraints of managed care, considerations of geography, or patient preference, the long-term care of an increasing number of renal transplant recipients has shifted away from the transplant center to the community-based nephrologist or internist. For optimal care to be delivered, it is important that the physicians managing these patients be cognizant of the complex and interacting medical issues involved in their care. Appropriate management can significantly prolong the life of the allograft as well as that of the patient. Guidelines for understanding and managing some of the more important and common general medical problems facing the long-term renal transplant recipient (eg, infectious complications, cardiovascular disease, hypertension, diabetes, hyperlipidemia, malignancy, pregnancy, bone disease, dental care, preventive care) are addressed in this section.  N. Ref:: 47

 

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[37]

TÍTULO / TITLE:  - Genetic variability and transplantation.

REVISTA / JOURNAL:  - Curr Opin Urol 2003 Mar;13(2):81-9.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.mou.0000058635.64616.41

AUTORES / AUTHORS:  - Marder B; Schroppel B; Murphy B

INSTITUCIÓN / INSTITUTION:  - Mount Sinai School of Medicine, New York, USA.

RESUMEN / SUMMARY:  - PURPOSE OF REVIEW: The purpose of this review is to summarize recent advances within the area of genetic polymorphisms with a specific emphasis on renal transplantation, and to discuss the potential clinical applications. RECENT FINDINGS: Due to recent advances in molecular techniques, there has been an abundance of publications describing genetic variability in molecules relevant to transplant outcome. Many studies are now demonstrating associations between polymorphisms in these candidate genes and outcomes in organ transplantation. SUMMARY: These studies emphasize the potential role of genetic variability in transplantation, and provide the rationale for large prospective studies to clearly define the potential benefits of genotyping in the risk stratification of transplant recipients.  N. Ref:: 91

 

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[38]

TÍTULO / TITLE:  - Anti-interleukin-2 receptor antibodies: basiliximab and daclizumab.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2001 Sep;16(9):1756-60.

AUTORES / AUTHORS:  - Pascual J; Marcen R; Ortuno J

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia, Hospital Ramon y Cajal, Universidad de Alcala, Carretera de Colmenar km 9, 100, E-28034 Madrid, España.  N. Ref:: 31

 

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[39]

TÍTULO / TITLE:  - A prospective study of rapid corticosteroid elimination in simultaneous pancreas-kidney transplantation: comparison of two maintenance immunosuppression protocols: tacrolimus/mycophenolate mofetil versus tacrolimus/sirolimus.

REVISTA / JOURNAL:  - Transplantation 2002 Jan 27;73(2):169-77.

AUTORES / AUTHORS:  - Kaufman DB; Leventhal JR; Koffron AJ; Gallon LG; Parker MA; Fryer JP; Abecassis MM; Stuart FP

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Division of Transplantation, Northwestern University Medical School, 675 N. St. Clair Street, Galter Pavilion, Suite 17-200, Chicago, IL 60611, USA.

RESUMEN / SUMMARY:  - BACKGROUND: We examined the feasibility of rapid corticosteroid elimination in simultaneous pancreas kidney transplantation. METHODS: Forty consecutive simultaneous pancreas-kidney (SPK) transplant recipients were enrolled in a prospective study in which antithymocyte globulin induction and 6 days of corticosteroids were administered along with tacrolimus and MMF (n=20) or tacrolimus and sirolimus (n=20). Mean+/-SD follow-up for recipients receiving tacrolimus/MMF and tacrolimus/sirolimus were 12.7+/-3.9 and 13.4+/-2.9 months, respectively. Patient and graft survival, and rejection rates were compared to an historical control group (n=86; mean follow-up 41.5+/-15.4 months) of SPK recipients that received induction and tacrolimus, MMF, and corticosteroids. RESULTS: Demographic characteristics of recipient and donor variables were similar among all groups. The 1-year actuarial patient, kidney, and pancreas survival rates in the 40 SPK transplant recipients with rapid corticosteroid elimination were 100, 100, and 100%, respectively. In the historical control group the 1-year actual patient, kidney, and pancreas survival rates were 96.5, 93.0, and 91.9%, respectively. The 1-year rejection-free survival rate recipients in the rapid steroid elimination group collectively was 97.5 vs 80.2% in the historical control group (P=0.034). At 6 and 12 months posttransplant the serum creatinine values remained stable in all groups. CONCLUSIONS: We conclude that chronic corticosteroid exposure is not required in SPK transplant recipients receiving antithymocyte globulin induction and maintenance immuno-suppression consisting of either tacrolimus and mycophenolate mofetil or tacrolimus and sirolimus.

 

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[40]

TÍTULO / TITLE:  - Rejection rate in living donor kidney transplantation with and without basiliximab in tacrolimus/mycophenolate mofetil-based protocol.

REVISTA / JOURNAL:  - Transplant Proc 2003 Mar;35(2):653-4.

AUTORES / AUTHORS:  - Rahamimov R; Yussim A; After T; Lustig S; Bar-Nathan N; Shaharabani E; Shapira Z; Shabthai E; Mor E

INSTITUCIÓN / INSTITUTION:  - Department of Transplantation, Rabin Medical Center, Beilinson Campus, Petah-Tiqwa, Israel. rutir@clalit.org.il

 

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[41]

TÍTULO / TITLE:  - Principles and clinical application of assessing alterations in renal elimination pathways.

REVISTA / JOURNAL:  - Clin Pharmacokinet 2003;42(14):1193-211.

AUTORES / AUTHORS:  - Tett SE; Kirkpatrick CM; Gross AS; McLachlan AJ

INSTITUCIÓN / INSTITUTION:  - School of Pharmacy, University of Queensland, Brisbane, Australia. s.tett@pharmacy.uq.edu.au

RESUMEN / SUMMARY:  - Drugs and metabolites are eliminated from the body by metabolism and excretion. The kidney makes the major contribution to excretion of unchanged drug and also to excretion of metabolites. Net renal excretion is a combination of three processes - glomerular filtration, tubular secretion and tubular reabsorption. Renal function has traditionally been determined by measuring plasma creatinine and estimating creatinine clearance. However, estimated creatinine clearance measures only glomerular filtration with a small contribution from active secretion. There is accumulating evidence of poor correlation between estimated creatinine clearance and renal drug clearance in different clinical settings, challenging the ‘intact nephron hypothesis’ and suggesting that renal drug handling pathways may not decline in parallel. Furthermore, it is evident that renal drug handling is altered to a clinically significant extent in a number of disease states, necessitating dosage adjustment not just based on filtration. These observations suggest that a re-evaluation of markers of renal function is required. Methods that measure all renal handling pathways would allow informed dosage individualisation using an understanding of renal excretion pathways and patient characteristics. Methodologies have been described to determine individually each of the renal elimination pathways. However, their simultaneous assessment has only recently been investigated. A cocktail of markers to measure simultaneously the individual renal handling pathways have now been developed, and evaluated in healthy volunteers. This review outlines the different renal elimination pathways and the possible markers that can be used for their measurement. Diseases and other physiological conditions causing altered renal drug elimination are presented, and the potential application of a cocktail of markers for the simultaneous measurement of drug handling is evaluated. Further investigation of the effects of disease processes on renal drug handling should include people with HIV infection, transplant recipients (renal and liver) and people with rheumatoid arthritis. Furthermore, changes in renal function in the elderly, the effect of sex on renal function, assessment of living kidney donors prior to transplantation and the investigation of renal drug interactions would also be potential applications. Once renal drug handling pathways are characterised in a patient population, the implications for accurate dosage individualisation can be assessed. The simultaneous measurement of renal function elimination pathways of drugs and metabolites has the potential to assist in understanding how renal function changes with different disease states or physiological conditions. In addition, it will further our understanding of fundamental aspects of the renal elimination of drugs.  N. Ref:: 135

 

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[42]

TÍTULO / TITLE:  - B19 virus infection in renal transplant recipients.

REVISTA / JOURNAL:  - J Clin Virol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.elsevier.com/gej-ng/29/46/32/show/Products/VIRUSINT/index.htt 

      ●● Cita: J Clinical Virology: <> 2003 Apr;26(3):361-8.

AUTORES / AUTHORS:  - Cavallo R; Merlino C; Re D; Bollero C; Bergallo M; Lembo D; Musso T; Leonardi G; Segoloni GP; Ponzi AN

INSTITUCIÓN / INSTITUTION:  - Virology Unit, Department of Public Health and Microbiology, University of Turin, Via Santena 9, 10126, Turin, Italy. rossana.cavallo@unito.it

RESUMEN / SUMMARY:  - BACKGROUND: B19 virus infection with persistent anaemia has been reported in organ transplant recipients. Detection of B19 virus DNA in serum is the best direct marker of active infection. OBJECTIVE: The present study evaluated the incidence and clinical role of active B19 virus infection in renal transplant recipients presenting with anaemia. STUDY DESIGN: Forty-eight such recipients were investigated by nested PCR on serum samples. The controls were 21 recipients without anaemia. Active HCMV infection was also investigated as a marker of high immunosuppression. RESULTS AND CONCLUSIONS: In 11/48 (23%) patients B19 virus DNA was demonstrated in serum versus only 1/21 (5%) of the controls. Ten of these 11 patients had already been seropositive at transplantation and active infection occurred in eight of them during the first 3 months after transplantation. The remaining patient experienced a primary infection 9 months after transplantation. Eight (73%) of these 11 patients displayed a concomitant HCMV infection and four (36%) showed increasing serum creatinine levels but none developed glomerulopathy; 3/11 (27%) recovered spontaneously from anaemia whereas 8/11 (73%) needed therapy. In conclusion, the relatively high occurrence (23%) of B19 virus infection in patients presenting with anaemia, suggests that it should be considered in the differential diagnosis of persistent anaemia in renal transplant recipients. Presence of the viral DNA should be assessed early from transplantation and the viral load should be monitored to follow persistent infection and better understand the relation between active infection and occurrence of anaemia, and to assess the efficacy of IVIG therapy and/or immunosuppression reduction in clearing the virus.  N. Ref:: 56

 

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[43]

TÍTULO / TITLE:  - Utility of intravenous immune globulin in kidney transplantation: efficacy, safety, and cost implications.

REVISTA / JOURNAL:  - Am J Transplant 2003 Jun;3(6):653-64.

AUTORES / AUTHORS:  - Jordan S; Cunningham-Rundles C; McEwan R

INSTITUCIÓN / INSTITUTION:  - Department of Pediatric Nephrology & Transplant Immunology, Cedars-Sinai Medical Center, Los Angeles, CA, USA. sjordan@cshs.org

RESUMEN / SUMMARY:  - Intravenous immunoglobulin preparations (IVIG) are known to be effective in the treatment of various autoimmune and inflammatory disorders into their immunomodulatory, immunoregulatory, and anti-inflammatory properties. Recently, IVIG has been utilized in the management of highly sensitized patients awaiting renal transplantation. The mechanisms of suppression of panel reactive antibodies (PRA) in patients awaiting transplantation are currently under investigation and appear to be related to anti-idiotypic antibodies present in IVIG preparations. In this review, the various immunomodulatory mechanisms attributable to IVIG and their efficacy in reducing PRAs will be described. In addition, the use of IVIG in solid organ transplant recipients will be reviewed. The adverse events, safety considerations, and economic impact of IVIG protocols for patients awaiting solid organ transplantation will be discussed.  N. Ref:: 67

 

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[44]

TÍTULO / TITLE:  - Thymic microchimerism correlates with the outcome of tolerance-inducing protocols for solid organ transplantation.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2001 Dec;12(12):2815-26.

AUTORES / AUTHORS:  - Noris M; Cugini D; Casiraghi F; Azzollini N; De Deus Viera Moraes L; Mister M; Pezzotta A; Cavinato RA; Aiello S; Perico N; Remuzzi G

INSTITUCIÓN / INSTITUTION:  - Department of Immunology and Clinics of Organ Transplantation, Mario Negri Institute for Pharmacological Research, via Gavazzeni 11, 24125 Bergamo, Italy. noris@marionegri.it

RESUMEN / SUMMARY:  - This study found that pretransplant infusion of donor peripheral blood leukocytes, either total leukocytes (peripheral blood leukocytes) or peripheral blood mononuclear cells (PBMC), under appropriate immunomodulating conditions was more effective than donor bone marrow (BM) in prolonging the survival of rats that received kidney grafts. A higher percentage of MHCII(+) cells was found in donor PBMC than in BM cells, and depletion of MHCII(+) cells from donor PBMC abolished their tolerogenic potential. By the analysis of microchimerism in rats infused with donor cells and killed at different time points thereafter, the better tolerogenic potential of leukocyte infusion related to a higher capability of these cells to engraft the recipient thymus. PCR analysis on OX6-immunopurified cells revealed the presence of donor MHCII(+) cells in the thymus of these animals. The role of intrathymic microchimerism was reinforced by findings that thymectomy at the time of transplant prevented tolerance induction by donor leukocytes. Donor DNA was found in the thymus of most long-term graft animals that survived, but in none of those that rejected their grafts. The presence of intrathymic microchimerism correlated with graft survival, and microchimerism in other tissues was irrelevant. PCR analysis of DNA from thymic cell subpopulations revealed the presence of donor MHCII(+) cells in the thymus of long-term surviving animals. Thus, in rats, donor leukocyte infusion is better than donor BM for inducing graft tolerance, defined by long-term graft survival, donor-specific T cell hyporesponsiveness, and reduced interferon gamma production. This effect appears to occur through migration of donor MHCII(+) cells in the host thymus.

 

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[45]

TÍTULO / TITLE:  - The effect of locally synthesised complement on acute renal allograft rejection.

REVISTA / JOURNAL:  - J Mol Med 2003 Jul;81(7):404-10. Epub 2003 Jun 25.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00109-003-0454-7

AUTORES / AUTHORS:  - Sacks S; Zhou W

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology and Transplantation, Guy’s Hospital, King’s College London, University of London, London, SE1 9RT, UK. steven.sacks@kcl.ac.uk

RESUMEN / SUMMARY:  - The complement system of components and receptors is one of the earliest forms of defence. Excessive or inappropriate activation can result in tissue damage, classically illustrated in immune-mediated nephritis. In addition, complement forms a bridge between innate and adaptive immunity, helping to prepare and focus T and B lymphocyte responses. More recent research in renal allograft models has shown that complement-inhibited and complement-deficient animals have reduced inflammatory injury and lowered antidonor immune responses. Furthermore, it is known that the transplanted kidney is a significant site of local synthesis of C3, although until recently the relative contribution of locally produced C3 to transplant injury was unknown. Current evidence indicates that defective local synthesis of C3 both reduces tissue injury and lowers the antidonor T cell response, substantially increasing graft survival. Among various possible explanations to account for these findings, the data favours a direct effect of complement on alloreactive T cell stimulation. Study of complement gene regulation by common immunosuppressive agents suggests that they do not influence local complement synthesis. Alternative approaches are therefore required to control the local effect of complement in the extravascular tissue compartment of the graft.  N. Ref:: 88

 

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[46]

- Castellano -

TÍTULO / TITLE:Trasplante renal en receptores mayores de 60 anos. Renal transplantation in donors over 60 years of age.

REVISTA / JOURNAL:  - Arch Esp Urol 2003 Mar;56(2):151-9.

AUTORES / AUTHORS:  - Gomez Vegas A; Blazquez Izquierdo J; Bocardo Fajardo G; San Jose Manso L; Fernandez Perez C; Silmi Moyano A; Resel Estevez L

INSTITUCIÓN / INSTITUTION:  - Catedra y Servicio de Urologia, Hospital Clinico San Carlos, Madrid, España.

RESUMEN / SUMMARY:  - OBJECTIVES: To evaluate the impact of receptor’s advanced age on kidney transplant outcomes. METHODS: We reviewed all transplants performed between January 1990 and December 1999. Among 570 patients receiving grafts, 115 patients were 60 years or older at the time of transplantation. We compared this group with receptors younger than 60 years. We studied possible prognostic variables and compared patient and graft outcomes. RESULTS: Mean age 63.81 (typical deviation (TD): 2.96). Mean follow-up time for elderly receptors was 41.6 months (TD: 26.58). 55.7% patients were males (p: 0.4). The most frequent cause for end stage renal disease was unknown etiology in group 1 and glomerular in the younger group (p: 0.01). 42% patients older than 60 years presented initial graft dysfunction, in comparison to 28.1% among younger than 60 (p: 0.006). Three-year graft survival was 90.42% for receptors 60 years old or older compared to 88.72% for group 2, without significant differences (p: 0.5). The most frequent graft loss etiology was patient death. (67.7%). (p = 0.005). Patient survival was 81.01% in group 1 and 95.25% in group 2, being differences significant (p < 0.001). CONCLUSIONS: Renal grafts in receptors over the age of 60 years show a greater incidence of delayed graft function, although it doesn’t seem to influence final graft survival. The most frequent cause for graft loss is receptor’s death. Receptor’s age does not represent a contraindication for transplant.  N. Ref:: 17

 

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[47]

TÍTULO / TITLE:  - Interpreting the mechanisms of continuous renal replacement therapy in sepsis: the peak concentration hypothesis.

REVISTA / JOURNAL:  - Artif Organs 2003 Sep;27(9):792-801.

AUTORES / AUTHORS:  - Ronco C; Tetta C; Mariano F; Wratten ML; Bonello M; Bordoni V; Cardona X; Inguaggiato P; Pilotto L; d’Intini V; Bellomo R

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, St. Bortolo Hospital, Vicenza, Italy. cronco@goldnet.it

RESUMEN / SUMMARY:  - Severe sepsis and septic shock are the primary causes of multiple organ dysfunction syndrome (MODS), which is the most frequent cause of death in intensive care unit patients. Many water-soluble mediators with pro- and anti-inflammatory action such as TNF, IL-6, IL-8, and IL-10 play a strategic role in septic syndrome. In intensive care medicine, blocking any one mediator has not led to a measurable outcome improvement in patients with sepsis. CRRT is a continuously acting therapy, which removes in a nonselective way pro- and anti-inflammatory mediators; “the peak concentration hypothesis” is the concept of cutting peaks of soluble mediators through continuous hemofiltration. Furthermore, there is evidence of increased efficacy of high-volume hemofiltration compared to conventional CVVH, and other blood purification techniques that utilize large-pore membranes or sorbent plasmafiltration are conceptually interesting.  N. Ref:: 91

 

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[48]

TÍTULO / TITLE:  - Eradication of parvovirus B19 infection after renal transplantation requires reduction of immunosuppression and high-dose immunoglobulin therapy.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2002 Oct;17(10):1840-2.

AUTORES / AUTHORS:  - Liefeldt L; Buhl M; Schweickert B; Engelmann E; Sezer O; Laschinski P; Preuschof L; Neumayer HH

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Charite, Humboldt-University Berlin, Germany. lutz.liefeldt@charite.de  N. Ref:: 17

 

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[49]

TÍTULO / TITLE:  - Limited dose monoclonal IL-2R antibody induction protocol after primary kidney transplantation.

REVISTA / JOURNAL:  - Am J Transplant 2002 Jul;2(6):568-73.

AUTORES / AUTHORS:  - Ahsan N; Holman MJ; Jarowenko MV; Razzaque MS; Yang HC

INSTITUCIÓN / INSTITUTION:  - Nephrology and Transplant Division, University of Medicine and Dentistry of New Jersey, New Brunswick 08904, USA. ahsanna@umdnj.edu

RESUMEN / SUMMARY:  - This study prospectively compared immunoprophylaxis with a single intraoperative dose (2 mg/kg) of monoclonal interleukin-2 receptor (IL-2R) antibody vs. noninduction in kidney transplant recipients treated with tacrolimus (FK 506), mycophenolate mofetil (MMF) and a prednisone-based immunosuppression regimen. One hundred recipients of first-kidney transplant were enrolled into the study to receive either anti-IL-2R monoclonal antibody, daclizumab (2 mg/kg intraoperatively, limited anti-IL-2R) or no induction (control). Each patient also received oral tacrolimus (dosed to target trough level 10-15 ng/mL), MMF (500 mg bid) and prednisone. The primary efficacy end-point was the incidence of biopsy proven acute rejection during the first 6 months post-transplant. The patients were also followed for 12-month graft function, and graft and patient survival rates. Other than the donor’s age being significantly lower in the control group, both groups were comparable with respect to age, weight, gender, race, human leukocyte antigen (HLA)-DR mismatch, panel reactive antibody (%PRA), cold ischemic time, cytomegalovirus (CMV) status, causes of renal failure, and duration and modes of renal replacement therapy (RRT). During the first 6 months, episodes of first biopsy confirmed acute rejection was 3/50 (6%) in the limited anti-IL-2R group and 8/50 (16%) in the controls (p < 0.05). Twelve-month patient 100/98 (%) and graft survival 100/96 (%) were not statistically different. The group receiving limited anti-IL-2R did not have any adverse reactions. Our study demonstrates that a limited (single) 2 mg/kg immunoprophylaxis dose with monoclonal IL-2R antibody (daclizumab) when combined with tacrolimus/MMF/steroid allows significant reduction in early renal allograft rejection to the single digit level. The therapy with anti-IL-2R antibody is simple and is well tolerated.

 

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[50]

TÍTULO / TITLE:  - The utility of monoclonal antibody therapy in renal transplantation.

REVISTA / JOURNAL:  - Transplant Proc 2002 May;34(3):797-800.

AUTORES / AUTHORS:  - Loertscher R

INSTITUCIÓN / INSTITUTION:  - Division of Transplantation, McGill University Health Centre, Montreal, Quebec, Canada. rolf.loertscher@mcgill.ca  N. Ref:: 37

 

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[51]

TÍTULO / TITLE:  - Role of prostanoids and endothelins in the prevention of cyclosporine-induced nephrotoxicity.

REVISTA / JOURNAL:  - Prostaglandins Leukot Essent Fatty Acids 2001 Apr-May;64(4-5):231-9.

      ●● Enlace al texto completo (gratuito o de pago) 1054/plef.2001.0265

AUTORES / AUTHORS:  - Darlametsos IE; Varonos DD

INSTITUCIÓN / INSTITUTION:  - Centre Franco-Hellenique de Recherches Biomedicales, Nikolaos Papanikolaou, Corporation of the Municipality Agrinion, Agrinion, 30100, Greece. darlamet@otenet.gr

RESUMEN / SUMMARY:  - Cyclosporine A nephrotoxicity includes both functional toxicity and histological changes, whose seriousness is dependent upon the dose and the duration of the drug administration. Several vasoactive agents have been found to be implicated in cyclosporine induced nephrotoxicity, among which prostanoids and endothelins are the most important. In previous studies we were able to prevent the early stage (7 days) of cyclosporine (37.4 micromol [45 mg]/kg/day) induced nephrotoxicity in rats either by the administration, i) of OKY-046, a thromboxane A(2)synthase inhibitor, ii) of ketanserine, an antagonist of S(2)serotonergic, a(1)adrenergic, and H(1)histaminergic receptors and iii) of nifedipine, a calcium channel blocker, or by diet supplementation either with evening primrose oil or fish oil. All these protective agents elevated ratios of excreted renal prostanoid vasodilators (prostaglandins E(2), 6ketoF(1 alpha)) to vasoconstrictor (thromboxane B(2)), a ratio which was decreased by the administration of cyclosporine alone. Nifedipine averted the cyclosporine induced increase of urinary endothelin-1 release. All protections were associated with the reinstatement of glomerular filtration rate forwards normal levels whereas renal damage defence, consisting of a decrease of the cyclosporine induced vacuolizations, was variable. Ketanserine and evening primrose oil were the only agents which prevented the animal body weight loss. These data suggest that prostanoids and endothelin-1 may mediate functional toxicity while thromboxane A(2)is involved the morphological changes too, provoked in the early stage of cyclosporine treatment. However, other nephrotoxic factors and additional mechanisms could also be implicated in the cyclosporine induced nephrotoxicity.  N. Ref:: 91

 

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[52]

TÍTULO / TITLE:  - Glycaemic control and graft loss following renal transplantation.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2001 Oct;16(10):1978-82.

AUTORES / AUTHORS:  - Thomas MC; Mathew TH; Russ GR  N. Ref:: 32

 

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[53]

TÍTULO / TITLE:  - Atypical generalized zoster with suspicious esophageal involvement and early relapse in an adult renal transplant recepient.

REVISTA / JOURNAL:  - Transplant Proc 2002 Jun;34(4):1174-7.

AUTORES / AUTHORS:  - Oh KH; Ahn C; Kim YS; Han JS; Kim S; Lee JS; Kim EC; Oh MD; Chung JH

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine, Seoul National University Hospital, Seoul, North Korea.  N. Ref:: 18

 

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[54]

TÍTULO / TITLE:  - Intrarenal synthesis of complement.

REVISTA / JOURNAL:  - Kidney Int 2001 Apr;59(4):1227-35.

AUTORES / AUTHORS:  - Zhou W; Marsh JE; Sacks SH

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology and Transplantation, Guy’s Hospital, London, England, United Kingdom.

RESUMEN / SUMMARY:  - During the past decade, research has shown that the kidney has the capacity to synthesize most of the activation pathway components of the complement cascade. As well as implying physiological roles in local clearance of immune complexes and defense against invasive organisms, an increasing amount of evidence indicates that the intrarenal synthesis of complement makes an important contribution in the pathogenesis of renal injury. Here we review this evidence and present a case for more definitive investigation of these functions.  N. Ref:: 76

 

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[55]

TÍTULO / TITLE:  - The role of newer monoclonal antibodies in renal transplantation.

REVISTA / JOURNAL:  - Transplant Proc 2001 Feb-Mar;33(1-2):1000-1.

AUTORES / AUTHORS:  - Vincenti F

INSTITUCIÓN / INSTITUTION:  - University of California, San Francisco, California, USA.  N. Ref:: 5

 

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[56]

TÍTULO / TITLE:  - Molecular mechanisms of renal allograft fibrosis.

REVISTA / JOURNAL:  - Br J Surg 2001 Nov;88(11):1429-41.

      ●● Enlace al texto completo (gratuito o de pago) 1046/j.0007-1323.2001.01867.x

AUTORES / AUTHORS:  - Waller JR; Nicholson ML

INSTITUCIÓN / INSTITUTION:  - Division of Transplant Surgery, University of Leicester, Leicester, UK. julian@waller79.fsnet.co.uk

RESUMEN / SUMMARY:  - BACKGROUND: Chronic graft nephropathy (CGN) remains the leading cause of renal allograft loss after the first year following transplantation. Histologically it is characterized by glomerulosclerosis, intimal hyperplasia and interstitial fibrosis. The pathogenesis is unclear, but is likely to involve both immunological and non-immunological factors. Despite improvements in short-term graft survival rates, new immunosuppressive regimens have made no impact on CGN. METHODS: A review of the current literature on renal transplantation, novel immunosuppression regimens and advances in the molecular pathogenesis of renal allograft fibrosis was performed. RESULTS AND CONCLUSION: Recent advances in understanding of the underlying molecular mechanisms involved suggest autocrine secretion of cytokines and growth factors, especially transforming growth factor beta, are associated with a change in fibroblast phenotype leading to the deposition of extracellular matrix. Repeated insults trigger upregulation of the tissue inhibitors of matrix metalloproteinases, favouring accumulation of extracellular matrix. To date, no drug has proved effective in inhibiting or reducing allograft fibrosis. The deleterious consequences of chronic immunosuppression on the development of such fibrosis are now recognized; newer immunosuppressive drugs, including rapamycin and mycophenolate mofetil, reduce profibrotic gene expression in both experimental and clinical settings, and offer potential strategies for prolonging allograft survival.  N. Ref:: 155

 

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[57]

TÍTULO / TITLE:  - Sequential protocol biopsies from renal transplant recipients show an increasing expression of active TGF beta.

REVISTA / JOURNAL:  - Transpl Int 2002 Dec;15(12):630-4. Epub 2002 Oct 19.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00147-002-0472-3

AUTORES / AUTHORS:  - Jain S; Mohamed MA; Sandford R; Furness PN; Nicholson ML; Talbot D

INSTITUCIÓN / INSTITUTION:  - University Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, LE5 4PW, UK. sj34@le.ac.uk

RESUMEN / SUMMARY:  - Chronic allograft nephropathy (CAN) is a major cause of graft loss after renal transplantation. Implicated in the pathogenesis of this complication is overproduction of the cytokine transforming growth factor beta (TGF beta). In this study we measured changes in CAN’s expression in stable patients early after transplantation, and studied links with established risk factors for CAN, such as delayed graft function, acute rejection, and cyclosporine exposure. We took biopsies from 40 renal allografts at time of transplantation (pre-perfusion), and then, using ultrasound guidance, at 1 week and 6 months after transplantation. An immunofluorescence technique was used to stain sections for active TGF beta. These were then assessed by semi-quantitative scanning laser confocal microscopy. There was very little variation in active TGF-beta expression among patients in their pre-perfusion biopsies. Expression had increased by 1 week and then very significantly by 6 months ( P<0.0001). Patients who suffered delayed graft function had increased TGF-beta expression at both time points. There was no difference regarding donor type, acute rejection, and immunosuppressive drug (cyclosporine or tacrolimus). There was no correlation between the amount of TGF-beta expression at any time-point and isotope glomerular filtration rate (GFR) at 12 months. This study demonstrated that in a group of stable renal allograft recipients, TGF-beta expression in the kidney increased after transplantation. As the study used protocol biopsies, this increase is unlikely to be due to acute events, and probably represents a genuine increase.

 

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[58]

TÍTULO / TITLE:  - The role of HLA class I and class II antibodies in renal transplantation.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2001;16 Suppl 6:150-2.

AUTORES / AUTHORS:  - Iniotaki-Theodoraki A

INSTITUCIÓN / INSTITUTION:  - National Tissue Typing Center, General Hospital of Athens G. Gennimatas, Athens, Greece.  N. Ref:: 15

 

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[59]

TÍTULO / TITLE:  - Acid-base and electrolyte management in continuous renal replacement therapy.

REVISTA / JOURNAL:  - Blood Purif 2002;20(3):262-8.

AUTORES / AUTHORS:  - Mehta RL

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, Department of Medicine, University of California San Diego Medical Center, San Diego, CA 92103-8342, USA. rmehta@ucsol.edu

RESUMEN / SUMMARY:  - Continuous renal replacement techniques are often utilized to manage acid-base and electrolyte problems in the critically ill patient. These techniques have an inherent capacity to manipulate the plasma composition and can be utilized efficiently to maintain homeostasis and metabolic control. Unfortunately, the efficacy of these techniques also permits wide variation in their use and can result in complications if they are not used appropriately. In most instances complications can be prevented by recognition of the operating principles and careful attention to detail. This article provides an overview of the principles of acid-base and electrolyte management with continuous renal replacement therapy.  N. Ref:: 27

 

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[60]

TÍTULO / TITLE:  - Role of transforming growth factor-beta1 in the progression of chronic allograft nephropathy.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2001;16 Suppl 1:114-6.

AUTORES / AUTHORS:  - Campistol JM; Inigo P; Larios S; Bescos M; Oppenheimer F

INSTITUCIÓN / INSTITUTION:  - Renal Transplant Unit, Hospital Clinic, Institut d’Investigacio Biomediques Agusti Pi i Sunyer, University of Barcelona, Barcelona, España.

RESUMEN / SUMMARY:  - Chronic allograft nephropathy is the principal cause of late graft loss after the first year of renal transplantation. Transforming growth factor-beta1 (TGF-beta1) is a key fibrogenetic cytokine involved in the fibrosis of a number of chronic diseases of the kidney and other organs, and recently evidence has shown that TGF-beta1 is involved in the pathogenesis of chronic renal allograft dysfunction. Production of TGF-beta1 in these circumstances may be modulated by the intrarenal renin-angiotensin system (angiotensin II induces TGF-beta1 production and secretion by the mesangial cells) and by a direct effect of cyclosporin A, which stimulates the synthesis and expression of TGF-beta1. In a prospective study of 14 renal transplant patients exhibiting chronic graft nephropathy, we demonstrated that treatment with losartan significantly decreased plasma levels of TGF-beta1 by >50%. There was a significant correlation (P=0.04) between the increase in circulating angiotensin II after 2 weeks and the decrease in plasma TGF-beta(1) at the end of the study period, suggesting that the degree of angiotensin II receptor blockade plays a decisive role in the synthesis of TGF-beta1. A significant decrease in circulating endothelin-1 (ET-1) levels also occurred during treatment with losartan, together with a decrease in proteinuria. In a randomized 2x2 crossover study, the effects of losartan and amlodipine on renal haemodynamics and on profibrogenetic cytokines were analysed. Whereas amlodipine increased the glomerular filtration rate (GFR) through an increase in the FF and P(G), losartan slightly decreased the GFR, but with a significant decrease in FF and P(G). With respect to the profibrogenetic cytokines, losartan decreased the plasma levels of TGF-beta1 and ET-1, while amlodipine did not significantly change TGF-beta1 and slightly increased ET-1.  N. Ref:: 16

 

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[61]

TÍTULO / TITLE:  - Cost-effectiveness analysis of basixilimab induction and calcineurin-sparing protocols in “old to old” programs using Markov models.

REVISTA / JOURNAL:  - Transplant Proc 2003 Jun;35(4):1324-5.

AUTORES / AUTHORS:  - Emparan C; Wolters H; Laukotter M; Dame C; Senninger N

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Division of Transplantation, Uniklinikum, Munster, Germany. cemparan@teleline.es

RESUMEN / SUMMARY:  - INTRODUCTION: Markov models are employed in economic analyses to evaluate all possible expectations in a dilemna. The introduction of a new clinical protocol (basiliximab induction with calcineurin-sparing protocols) for a group of kidney transplant recipients receiving organs from marginal donors was validated with a Markov simulation model. HYPOTHESIS: Calcineurin-sparing protocols using anti-IL-2/antibody induction (Simulect) show a beneficial effect on initial kidney function, reducing transplantation costs reception based upon mean length of stay, mean admission cost, and incidences of delayed graft function and complications during the first month after transplant. PATIENTS AND METHODS: A Markov simulation model was established following three different chains. A calcineurin-free regimen with basiliximab induction (chain A), a calcineurin-sparing protocol with basiliximab induction (chain B), and a conventional immunosuppressive regimen (chain C). After designing the Markov chain and cohorts, 31 patients from the “old to old” program were assigned to each chain eight to chain A, (eight to chain B, and 15 to chain C). A month after transplantation a cost-benefit study was performed guided by the three branches of the Markov model. RESULTS: The Markov model showed a benefit of induction therapies in elderly patients. A cost-benefit model showed that after a month there was a clear benefit from Calcineurin=free plus basiliximab induction therapies, with a slight benefit from calcineurin-sparing protocols. CONCLUSIONS: Markov models are extremely useful when introducing new clinical therapies. In our transplant program, a cost-effective analysis of outcomes in old patients using the Markov model showed a clear benefit of calcineurin-sparing protocols with basixilimab induction.

 

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[62]

TÍTULO / TITLE:  - Genitourinary tuberculosis after renal transplantation: report of 3 cases and review.

REVISTA / JOURNAL:  - Clin Infect Dis 2001 Feb 15;32(4):662-6. Epub 2001 Feb 7.

AUTORES / AUTHORS:  - Dowdy L; Ramgopal M; Hoffman T; Ciancio G; Burke G; Roth D; Mies C; Jones B; Miller J

INSTITUCIÓN / INSTITUTION:  - Division of Infectious Diseases, Department of Medicine, University of Miami School of Medicine, Miami, FL 33136, USA. ldowdy@med.miami.edu

RESUMEN / SUMMARY:  - Mycobacterium tuberculosis infection of the genitourinary tract is an uncommon disease in renal transplant recipients and presentation is atypical. Genitourinary tuberculosis is associated with graft rejection, and this diagnosis should be considered for renal transplant recipients with unexplained fever and constitutional symptoms.  N. Ref:: 8

 

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[63]

TÍTULO / TITLE:  - Steroid-free immunosuppression in kidney transplantation: an editorial review.

REVISTA / JOURNAL:  - Am J Transplant 2002 Jan;2(1):19-24.

AUTORES / AUTHORS:  - Hricik DE

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, Case Western Reserve University School of Medicine, University Hospitals of Cleveland, Ohio 44106, USA. deh5@po.cwru.edu  N. Ref:: 33

 

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[64]

TÍTULO / TITLE:  - Factor V Leiden mutation: potential thrombogenic role in renal vein, dialysis graft and transplant vascular thrombosis.

REVISTA / JOURNAL:  - Curr Opin Nephrol Hypertens 2001 May;10(3):409-14.

AUTORES / AUTHORS:  - Wuthrich RP

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, Department of Medicine, Kantonsspital, St. Gallen, Switzerland. rpw@kssg.ch

RESUMEN / SUMMARY:  - Factor V is an important blood coagulation factor, the procoagulatory activity of which is inhibited by activated protein C. The factor V Leiden mutation is due to a single base-pair change (G1691A), which alters the initial cleavage site for activated protein C. The impaired degradation of factor V by activated protein C yields a hypercoagulable state that confers a lifelong increased risk of thrombosis in heterozygous and homozygous individuals. The factor V Leiden mutation represents the most common cause of inherited thrombophilia, and enhances the risk for venous thrombosis by approximately sevenfold. In normal Western populations, heterozygosity for the factor V Leiden mutation is present in 2-5%, whereas in patients with venous thrombosis and a family history of thrombotic disease this figure may reach 50-60%. The presence of the mutation markedly increases the risk for renal vein thrombosis, particularly in neonates. Heterozygosity for factor V Leiden mutation does not appear to be a major risk factor for dialysis access clotting. The presence of factor V Leiden mutation is most devastating in kidney transplant recipients. In these patients the mutation predisposes to renal transplant vein thrombosis and early graft loss. The risk for acute vascular rejection is also enhanced in transplant recipients who are heterozygous for the mutation. Routine screening for factor V Leiden mutation by polymerase chain reaction, and appropriate perioperative and postoperative anticoagulation after renal transplantation might be a valuable strategy to prevent thromboembolic complications in transplant recipients.  N. Ref:: 47

 

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[65]

TÍTULO / TITLE:  - Cellular and molecular parameters in human renal allograft rejection.

REVISTA / JOURNAL:  - Clin Biochem 2001 Feb;34(1):29-34.

AUTORES / AUTHORS:  - Kamoun M

INSTITUCIÓN / INSTITUTION:  - Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104-4283, USA. malekkam@mail.med.upenn.edu

RESUMEN / SUMMARY:  - Acute rejection of human renal allografts is frequent postransplantation complication. In addition, it is a risk factor for chronic rejection, the most common cause of failure of long-term allografts. Renal allografts are rejected as a result of an immune response directed against alloantigens on the graft that are absent from the host, and the most important of these are the HLA antigens. The application of molecular diagnostic methods has revealed a differential intra-renal gene expression of cytokines, chemokines and their receptors, and cytotoxic attack molecules in acute and chronic rejection processes. Differential expression of T cell costimulatory molecules B7 and CD40/CD40L, and endothelium adhesion molecules ICAM-1 and VCAM-1 has also been reported during acute rejection. These molecules play an important role in mediating the recruitment of lymphocytes into rejecting allografts and costimulation of T cell activation. Based on experimental data, it seems that it is likely that the blockade of T cell costimulatory pathways can be used in human in the future to selectively prevent transplant rejection without generally suppressing the immune system.  N. Ref:: 45

 

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[66]

TÍTULO / TITLE:  - HLA-specific alloantibodies and renal graft outcome.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2001 May;16(5):897-904.

AUTORES / AUTHORS:  - Sumitran-Holgersson S

INSTITUCIÓN / INSTITUTION:  - Division of Clinical Immunology, Karolinska Institutet, Huddinge University Hospital, Huddinge, Sweden.

RESUMEN / SUMMARY:  - HLA-specific humoral immunity, as a result of recipient allosensitization, induces hyperacute rejection of allogenic kidney grafts. Cross-match tests are performed to avoid this complication. However, current techniques do not allow determination of HLA-specificity of donor-reactive antibodies in the acute cadaver-donor situation. New methods are described and discussed in this report as well as the alloantibody specificities that are of clinical importance. Alloantibodies not only mediate hyperacute rejection but may also participate in the acute rejection of organ grafts. Clinical associations between early immunological complications, such as acute rejection, in heart, liver and kidney allografted patients and pre-transplantation humoral alloimmunity emphasize the need for proper determination of donor-specific humoral immunity prior to transplantation.  N. Ref:: 35

 

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[67]

TÍTULO / TITLE:  - Role of anti-interleukin-2 receptor antibodies in kidney transplantation.

REVISTA / JOURNAL:  - BioDrugs 2001;15(10):655-66.

AUTORES / AUTHORS:  - Cibrik DM; Kaplan B; Meier-Kriesche HU

INSTITUCIÓN / INSTITUTION:  - University of Michigan, Ann Arbor, Michigan 48109-0704, USA. dcibrik@umich.edu

RESUMEN / SUMMARY:  - From the early 1960s, the mainstay of immunosuppression for kidney transplantation has been corticosteroids. Since then, many new drugs have been developed to maintain the renal allograft. Current maintenance immunosuppression commonly consists of corticosteroids, antiproliferative agents and calcineurin inhibitors (e.g. cyclosporin). More recently, antihuman antibodies, either monoclonal or polyclonal, have been developed to use for induction at the time of transplantation or to treat rejection. With the advances in molecular technology, a new class of antihuman antibodies [the anti-interleukin-2 receptor (IL-2R) antibodies] has emerged that incorporate a murine antigen-binding site on to a human immunoglobulin backbone. Such methodology creates antihuman antibodies with high affinity for the epitope and with prolonged serum antibody half-lives. Interleukin-2 and its receptor are central to lymphocyte activation and are the main targets of calcineurin inhibitors. In addition, the anti-IL-2R antibodies inhibit a key target in immune activation. Daclizumab and basiliximab have been shown to significantly reduce the incidence of acute rejection in kidney transplantation. Since these anti-IL-2R antibodies are well tolerated and since calcineurin inhibitors are intrinsically nephrotoxic, anti-IL-2R antibodies have been used in an attempt to avoid cyclosporin after transplantation. Data from clinical trials seem to indicate that the addition of an anti-IL-2R antibody is not sufficient to warrant complete withdrawal of calcineurin inhibitors for more than a very short period after transplantation. A more promising role for anti-IL-2R antibodies may be in renal transplant recipients with delayed graft function (DGF). Recent data on the use of either low-dose calcineurin inhibitors or sirolimus (rapamycin) in conjunction with the anti-IL-2R antibodies for patients with DGF showed no increased risk of acute rejection. Long-term graft survival with use of these low-dose calcineurin inhibitor protocols has yet to be established.  N. Ref:: 41

 

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[68]

TÍTULO / TITLE:  - Chromomycosis due to Exophiala jeanselmei in a renal transplant recipient.

REVISTA / JOURNAL:  - Eur J Dermatol 2003 May-Jun;13(3):305-7.

AUTORES / AUTHORS:  - Pena-Penabad C; Duran MT; Yebra MT; Rodriguez-Lozano J; Vieira V; Fonseca E

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Complejo Hospitalario Juan Canalejo, Servicio de Dermatologia, Xubias de Arriba, 84, 15006. a Coruna, España.

RESUMEN / SUMMARY:  - Chromomycosis is a rare mycotic infection that is more frequent in tropical and subtropical regions. Dematiaceous fungi are the causal agents of this mycosis. Several cases of chromomycosis in organ transplant recipients have been reported. We present a case of chromomycosis by Exophiala jeanselmei in a Spanish male who had received a renal transplant several months previously, and was receiving treatment with tacrolimus, prednisone and mycophenolate mofetil. Very few cases of chromomycosis due to Exophiala have been reported, and this is, to our knowledge, the first European case.  N. Ref:: 16

 

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[69]

TÍTULO / TITLE:  - Postural renal transplant obstruction: a case report and review of the literature.

REVISTA / JOURNAL:  - Clin Nucl Med 2001 Aug;26(8):673-6.

AUTORES / AUTHORS:  - Cohn DA; Gruenewald S

INSTITUCIÓN / INSTITUTION:  - Department of Nuclear Medicine and Ultrasound, Westmead Hospital, Westmead, New South Wales, Australia.

RESUMEN / SUMMARY:  - A 48-year-old woman underwent cadaveric renal transplantation for end-stage renal failure secondary to polycystic kidney disease. Nine months after transplantation, intermittent renal dysfunction and severe graft hydronephrosis developed despite the presence of a ureteric stent. A Tc-99m MAG3 scan performed with the patient standing showed complete transplant obstruction. Rapid tracer clearance with progressive bladder filling was present when the patient was imaged in the supine position. Ureteric obstruction is the most common urologic complication of renal transplantation. However, postural ureteric obstruction has been described only rarely. This case indicates that posture may affect ureteric patency and highlights this potential pitfall in the evaluation of intermittent graft dysfunction by diuretic renography.  N. Ref:: 10

 

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[70]

TÍTULO / TITLE:  - Molecular diagnosis of viral infections in renal transplant recipients.

REVISTA / JOURNAL:  - Curr Opin Nephrol Hypertens 2002 Nov;11(6):665-72.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.mnh.0000040054.33359.e8

AUTORES / AUTHORS:  - Middeldorp JM

INSTITUCIÓN / INSTITUTION:  - Department of Pathology, VU Medical Center, Amsterdam, the Netherlands. j.middeldorp@vumc.nl

RESUMEN / SUMMARY:  - PURPOSE OF REVIEW: To discuss biological and methodological aspects of virus infection monitoring in the renal transplant setting. RECENT FINDINGS: New insights on the molecular pathogenesis of acute and persistent virus infections and rapid developments in real-time monitoring techniques are changing the current diagnostic routine. Accurate risk-assessment prior to transplantation and quantitative monitoring of parameters that reflect virus activity in the post-transplant period, including genome load fluctuations and aberrant viral mRNA or protein expression, provide early signs of undesired viral behaviour and allow pre-emptive therapeutic intervention. As opposed to prophylactic administration of antiviral drugs, a pre-emptive approach is more selective and will allow for antiviral immune responses to build, which may have a long-term beneficial effect. In addition, these virus-monitoring techniques allow for on-line assessment of therapeutic efficacy and rapid identification of emerging resistant strains. The combination of virus-monitoring techniques with rapid assessment of host immune responses using FACS and ELISPOT techniques, will improve overall patient management and long-term survival. SUMMARY: Viral infections continue to be a significant complication in the transplant setting. Diagnostic monitoring allows timely and accurate therapeutic intervention. Knowledge of pathogenic mechanisms leading to disease is important for clinical decision making as well as for the selection of appropriate molecular parameters discriminating normal and disease-related activity of human pathogenic viruses. The increasing availability of effective antiviral drugs permits pre-emptive intervention that strongly depends on accurate viral monitoring procedures.  N. Ref:: 57

 

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[71]

TÍTULO / TITLE:  - TGF-beta1 expression and chronic allograft nephropathy in protocol kidney graft biopsy.

REVISTA / JOURNAL:  - Physiol Res. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.biomed.cas.cz/physiolres/ 

      ●● Cita: Physiological Research: <> 2003;52(3):353-60.

AUTORES / AUTHORS:  - Viklicky O; Matl I; Voska L; Bohmova R; Jaresova M; Lacha J; Lodererova A; Striz I; Teplan V; Vitko S

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. ivo.matl@medicon.cz

RESUMEN / SUMMARY:  - Chronic allograft nephropathy (CAN) represents a frequent and irreversible cause of long-term renal graft loss. TGF-beta1 is a key profibrogenic cytokine associated with CAN pathogenesis. Because of clinical diagnostic inaccuracy, protocol biopsy has been suggested to be a beneficial method for early CAN detection. Protocol core biopsy was carried out in 67 consecutive cyclosporine-based immunosuppression-treated kidney transplant recipients with stable renal function 12 months after renal transplantation. Biopsy specimens were analyzed morphologically according to Banff-97’ criteria and immunohistologically for TGF-beta1 staining. The data obtained were correlated with plasma TGF-beta1 levels and clinical data. CAN (grade I-III) was found in 51 patients (76 %). CAN grade I was found to be the most frequent one (44 %). A normal finding within the graft was made in only 12 patients (18 %). Clinically silent acute rejection Banff IA was present in 4 patients (6 %). In 8 patients (12 %) with CAN, borderline changes were present. We found a significant correlation between CAN grade and creatinine clearance, as measured by the Cockroft-Gault formula (p<0.01) as well as body mass index (p<0.01). There was a significant correlation between chronic vasculopathy (Banff cv) and creatinine clearance, and between the degree of TGF-beta1 staining and chronic vasculopathy (p<0.01). There were no relations between morphological findings and TGF-beta1 plasma levels, cyclosporine levels, plasma lipids, HLA-mismatches, panel reactive antibodies (PRA), proteinuria, and the donor’s age. In conclusion, CAN is a frequent finding in protocol kidney graft biopsies 12 months after transplantation. TGF-beta1 tissue expression is linked with chronic vasculopathy.

 

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[72]

TÍTULO / TITLE:  - Molecular mechanisms of human embryogenesis: developmental pathogenesis of renal tract malformations.

REVISTA / JOURNAL:  - Pediatr Dev Pathol 2002 Mar-Apr;5(2):108-29.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s10024-001-0141-z

AUTORES / AUTHORS:  - Woolf AS; Winyard PJ

INSTITUCIÓN / INSTITUTION:  - Nephro-Urology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.

RESUMEN / SUMMARY:  - The focus of this review is the normal and abnormal development of the kidney and lower urinary tract; for convenience, we will refer to the whole system as the renal tract. The content represents a convergence among the clinical disciplines of histopathology, nephrology, and urology as well the basic sciences of developmental biology and molecular genetics. The story has considerable clinical relevance since diverse renal tract malformations are increasingly detected on fetal ultrasound screening and constitute major causes of chronic renal failure necessitating dialysis and kidney transplantation in children. Evidence is emerging that at least some of these disorders have a defined genetic basis; in others, an abnormal embryonic, or even maternal, environment may contribute to the pathogenesis. This field of study is frequently updated, with new discoveries being made almost every week. Hence this review can not be exhaustive or definitive, but instead highlights some specific areas of interest.  N. Ref:: 235

 

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[73]

TÍTULO / TITLE:  - Homocysteine levels among transplant recipients: effect of immunosuppressive protocols.

REVISTA / JOURNAL:  - Transplant Proc 2001 Sep;33(6):2945-6.

AUTORES / AUTHORS:  - Mor E; Helfmann L; Lustig S; Bar-Nathan N; Yussim A; Sela BA

INSTITUCIÓN / INSTITUTION:  - Department of Transplantation, Rabin Medical Center, Petach-Tikva, Israel.

 

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[74]

TÍTULO / TITLE:  - Routine renin-angiotensin system blockade in renal transplantation?

REVISTA / JOURNAL:  - Curr Opin Nephrol Hypertens 2002 Jan;11(1):1-10.

AUTORES / AUTHORS:  - Remuzzi G; Perico N

INSTITUCIÓN / INSTITUTION:  - Department of Immunology and Clinic of Organ Transplantation, Ospedali Riuniti di Bergamo and Mario Negri Institute for Pharmacological Research, Bergamo, Italy. gremuzzi@marionegri.it

RESUMEN / SUMMARY:  - There is ample evidence to support the recommendation of renin-angiotensin system blockade therapy as the standard of care for strategies aimed at preserving renal function in chronic renal disease. Nevertheless, despite the well established antihypertensive effects of these drugs, the use of renin-angiotensin system blockers in renal transplantation has been quite limited so far, nephrologists being afraid of the possibility of inducing renal insufficiency in patients with a single kidney transplant. However, current knowledge of the ability of these agents to control blood pressure and urinary protein excretion, as well as post-transplant erythrocytosis, effectively in kidney transplant recipients suggests that it is now time to apply renin-angiotensin system blockers to the field of renal transplantation.  N. Ref:: 105

 

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[75]

TÍTULO / TITLE:  - Kidney transplantation and ANCA-associated vasculitis.

REVISTA / JOURNAL:  - Cleve Clin J Med. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.ccjm.org/ 

      ●● Cita: Cleveland Clinic J. of Medicine: <> 2002;69 Suppl 2:SII143-5.

AUTORES / AUTHORS:  - van der Woude FJ

INSTITUCIÓN / INSTITUTION:  - Vth Medical University Clinic, Klinikum Mannheim, Heidelberg University, Mannheim, Germany. Fokko.van-der-Woude@med5.ma.uni-heidelberg.de  N. Ref:: 26

 

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[76]

TÍTULO / TITLE:  - Beyond the crossmatch: successful renal transplantation after the elimination of anti-donor antibodies.

REVISTA / JOURNAL:  - Curr Opin Nephrol Hypertens 2002 Nov;11(6):583-8.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.mnh.0000040041.55337.82

AUTORES / AUTHORS:  - Cohen DJ  N. Ref:: 56

 

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[77]

TÍTULO / TITLE:  - Cell-free DNA in urine: a marker for kidney graft rejection, but not for prenatal diagnosis?

REVISTA / JOURNAL:  - Ann N Y Acad Sci 2001 Sep;945:250-7.

AUTORES / AUTHORS:  - Zhong XY; Hahn D; Troeger C; Klemm A; Stein G; Thomson P; Holzgreve W; Hahn S

INSTITUCIÓN / INSTITUTION:  - Department of Obstetrics and Gynecology, University of Basel, Switzerland.

RESUMEN / SUMMARY:  - Intrigued by the rapid clearance of free fetal DNA from the maternal circulation, we have investigated whether this fetal genetic material could be cleared via the kidney. For this purpose, we examined for the presence of Y chromosome-specific DNA sequences in urine samples obtained from 8 women pregnant with male fetuses. No male-specific sequences could be detected, despite the use of a very sensitive nested PCR assay nor a highly reproducible real-time PCR assay. We did, however, detect maternal DNA sequences. To determine if this cell-free DNA was derived from the kidney or another source, we next examined urine from female kidney transplant patients who had received male kidneys. Y chromosome-specific sequences were indeed detectable by both nested and real-time PCR in these samples, thereby confirming a recent report describing urinary DNA microchimerism. Quantitative analysis of serially obtained samples furthermore suggests that transplant-derived sequences are elevated during periods of graft rejection. These results imply that the measurement of graft-derived urinary DNA may serve as a new marker for kidney graft tolerance.  N. Ref:: 18

 

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[78]

TÍTULO / TITLE:  - Organ transplantation in the vasculitides.

REVISTA / JOURNAL:  - Curr Opin Rheumatol 2003 Jan;15(1):22-8.

AUTORES / AUTHORS:  - Schmitt WH; van der Woude FJ

INSTITUCIÓN / INSTITUTION:  - Vth Medical Clinic (Nephrology, Endocrinology), University-Clinic Mannheim, Faculty of Clinical Medicine of The University of Heidelberg, Germany. wilhelm.schmitt@med5.ma.uni-heidelberg.de

RESUMEN / SUMMARY:  - Despite important therapeutic improvements, permanent organ failure may develop in primary systemic vasculitides and affect the heart, the lungs, and especially the kidneys. In systemic vasculitides associated with antineutrophil cytoplasmic antibodies (AASV), end-stage renal failure develops in 20% of cases. Renal transplantation became a beneficial option in these patients, with a graft and patient survival comparable to that in nondiabetic patients. This review summarizes the current knowledge on indications and contraindications for renal transplantation in AASV and discusses the impact of posttransplant immunosuppression on the course of the patients.  N. Ref:: 57

 

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[79]

TÍTULO / TITLE:  - Anti-interleukin-2 receptor antibodies for the prevention of rejection in pediatric renal transplant patients: current status.

REVISTA / JOURNAL:  - Paediatr Drugs 2003;5(10):699-716.

AUTORES / AUTHORS:  - Swiatecka-Urban A

INSTITUCIÓN / INSTITUTION:  - Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. Agnieszka.Swiatecka-Urban@Dartmouth.edu

RESUMEN / SUMMARY:  - The anti-interleukin-2 receptor (anti-IL-2R) antibody therapy is an exciting approach to the prevention of acute rejection after renal allograft transplantation whereby immunosuppression is exerted by a selective and competitive inhibition of IL-2-induced T cell proliferation, a critical pathway of allorecognition. The anti-IL-2R antibodies specifically block the alpha-subunit of the IL-2R on activated T cells, and prevent T cell proliferation and activation of the effector arms of the immune system. The anti-IL-2R antibodies are used as induction therapy, immediately after renal transplantation, for prevention of acute cellular rejection in children and adults. During acute rejection, the IL-2Ralpha chain is no longer expressed on T cells; thus, the antibodies cannot be used to treat an existing acute rejection. Two anti-IL-2R monoclonal antibodies are currently in clinical use: daclizumab and basiliximab. In placebo-controlled phase III clinical trials in adults, daclizumab and basiliximab in combination with calcineurin inhibitor-based immunosuppression, significantly reduced the incidence of acute rejection and corticosteroid-resistant acute rejection without increasing the risk of infectious or malignant complications, and neither antibody was associated with the cytokine-release syndrome. Children who receive calcineurin inhibitors and corticosteroids for maintenance immunosuppression, as well as children who receive augmented immunosuppression to treat acute rejection, are at increased risk of growth impairment, hypertension, hyperlipidemia, lymphoproliferative disorders, diabetes mellitus, and cosmetic changes. In older children, the cosmetic adverse effects frequently reduce compliance with the treatment, and subsequently increase the risk of allograft loss. Being effective and well tolerated in children, the anti-IL-2R antibodies reduce the need for calcineurin inhibitors while maintaining the overall efficacy of the regimen; thus, the anti-IL-2R antibodies increase the safety margin (less toxicity, fewer adverse effects) of the baseline immunosuppression. Secondly, the anti-IL-2R antibodies decrease the need for corticosteroids and muromonab CD3 (OKT3) in children as a result of decreased incidence of acute rejection. The recommended pediatric dose of daclizumab is 1 mg/kg intravenously every 14 days for five doses, with the first dose administered within 24 hours pre-transplantation. This administration regimen maintains daclizumab levels necessary to completely saturate the IL-2Ralpha (5-10 microg/mL) in children for at least 12 weeks.The recommended pediatric dose of basiliximab for recipients <35 kg is 10 mg, and 20 mg for recipients > or =35 kg, intravenously on days 0 and 4 post-transplantation. This administration regimen maintains basiliximab levels necessary to completely saturate the IL-2Ralpha (>0.2 microg/mL) in children for at least 3 weeks.  N. Ref:: 88

 

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[80]

TÍTULO / TITLE:  - Lymphocyte costimulatory receptors in renal disease and transplantation.

REVISTA / JOURNAL:  - J Nephrol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.jnephrol.com/ 

      ●● Cita: Journal of Nephrology: <> 2002 Jan-Feb;15(1):7-16.

AUTORES / AUTHORS:  - Biancone L; Deambrosis I; Camussi G

INSTITUCIÓN / INSTITUTION:  - Chair of Nephrology, University of Turin, Italy.

RESUMEN / SUMMARY:  - Cell-to-cell signal exchange during antigen presentation deeply influences the profile and extent of the immune response. Together with the TCR/MHC-mediated signal, accessory signals are provided to the T cell by the antigen-presenting cell (APC), through specific receptor-ligand interactions that represent indispensable costimulation for T-cell activation and survival. The main costimulatory pathways are the B7 family members and the CD40-CD154 receptor-ligand pair. B7-1 and B7-2 costimulate T-cells by binding to CD28. Their binding is prevented by the neoexpression of CTLA4, a CD28 homologue that can deliver a negative signal. Another CD28-like molecule, called ICOS (inducible costimulator), has been described and binds B7RP-1, a third member of the B7 family, but not B7-1 and B7-2. The CD40-CD154 interaction works as a two way costimulatory system by triggering activation signals to both T-cell and APCs. Its importance is highlighted by the discovery that mutations of the CD154 gene are responsible for a severe human immunodeficiency. Disruption of the natural costimulatory interaction was highly effective for prevention and treatment in several experimental models of autoimmune disease and transplant rejection. This review focuses on the most significant advances in understanding the physiopathological events involving costimulatory molecules, and their impact on renal diseases and transplantation.  N. Ref:: 65

 

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[81]

TÍTULO / TITLE:  - From renal amyloid deposits to the identification of the culprit genes.

REVISTA / JOURNAL:  - J Nephrol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.jnephrol.com/ 

      ●● Cita: Journal of Nephrology: <> 2003 May-Jun;16(3):427-30.

AUTORES / AUTHORS:  - Pirson Y

INSTITUCIÓN / INSTITUTION:  - Nephrology Department, Cliniques St Luc, Universite Catholique de Louvain, Brussels, Belgium. pirson@nefr.ucl.ac.be

RESUMEN / SUMMARY:  - Hereditary amyloidoses with renal involvement are classified in two groups. The first group is a growing family of autoinflammatory disorders characterized by recurrent fever attacks. Amyloidosis is caused by the deposition of amyloid A (AA) protein, which is a degradation product of a normal serum acute-phase protein: serum amyloid A (SAA). The prototype is familial Mediterranean fever (FMF). TNF Receptor Associated Periodic Syndrome (TRAPS) is a recently recognized periodic fever syndrome, differing from FMF in several characteristics: autosomal-dominant transmission, longer duration of attacks, and lack of response to colchicine prophylaxis. The second group comprises a variety of disorders, each characterized by the deposition of a specific mutant protein. The prototype is transthyretin amyloidosis (TTR). Identification of the form of amyloidosis has clinical implications. Therefore, in a patient with a history of recurrent fever attacks and AA amyloidosis, a diagnosis of FMF or TRAPS dictates appropriate genetic counseling and management. In patients with renal amyloidosis without a history of fever, identification of the mutant protein is therapeutically crucial; therefore, when the cell type that produces the precursor is (exclusively or mainly) the hepatocyte, a liver transplantation is to be considered.  N. Ref:: 24

 

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[82]

TÍTULO / TITLE:  - Management of selected lipid abnormalities: hypertriglyceridemia, isolated low HDL-cholesterol, lipoprotein(a), and lipid abnormalities in renal diseases and following solid organ transplantation.

REVISTA / JOURNAL:  - Cardiol Clin 2003 Aug;21(3):377-92.

AUTORES / AUTHORS:  - Rosas S; Szapary P; Rader DJ

INSTITUCIÓN / INSTITUTION:  - University of Pennsylvania Medical Center, 654 BRBII/III Labs, 421 Curie Boulevard, Philadelphia, PA 19104-6160, USA.

RESUMEN / SUMMARY:  - Although the focus in treating lipid disorders is on reducing LDL-C levels, additional lipid-related independent risk factors, such as TG, HDL-C, and Lp(a) levels, should be used clinically to assess cardiovascular risk. Decisions to initiate drug therapy for LDL-C reduction may be influenced by levels of these other lipoprotein fractions. Data supporting intervention to modify these factors are less abundant than for LDL-C reduction, but in certain circumstances. drug therapy targeted at TGs or HDL-C may be appropriate. Patients who have nephrotic syndrome and end-stage renal disease are at particularly high risk for the development of CVD and should be treated aggressively for their lipid disorders. Finally, solid organ transplant recipients are almost always hyperlipidemic and appropriate therapy could reduce cardiovascular events.  N. Ref:: 146

 

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[83]

TÍTULO / TITLE:  - Complement in renal transplantation.

REVISTA / JOURNAL:  - Nephron Clin Pract 2003;95(1):c3-8.

      ●● Enlace al texto completo (gratuito o de pago) 1159/000073012

AUTORES / AUTHORS:  - Chowdhury P; Zhou W; Sacks SH

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology and Transplantation, Guy’s Hospital, King’s College, University of London, London, UK.

RESUMEN / SUMMARY:  - Previous research and therapy in renal transplantation largely focused on the cellular arm of the adaptive immune response. Evidence is emerging that innate immune mechanisms, particularly complement, play a greater role in inflammatory and immune responses against the graft than has been previously recognized. Alternative complement pathway activation appears to mediate renal ischaemia/reperfusion injury, and proximal tubular cells may be both the source and the site of attack of complement components in this setting. Locally produced complement also plays a role in the development of both cellular and antibody-mediated immune responses against the graft. C4d staining has emerged as a useful marker of humoral rejection both in the acute and in the chronic setting and led to renewed interest in the significance of anti-donor antibody formation. A number of therapies are in development which inhibit complement or reduce local synthesis, and may lead to an improved clinical outcome following renal transplantation.  N. Ref:: 25

 

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[84]

TÍTULO / TITLE:  - Transplantation of kidneys from HCV-positive donors: a safe strategy?

REVISTA / JOURNAL:  - J Nephrol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.jnephrol.com/ 

      ●● Cita: Journal of Nephrology: <> 2003 Sep-Oct;16(5):617-25.

AUTORES / AUTHORS:  - Fabrizi F; Bunnapradist S; Lunghi G; Martin P

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, Dialysis, Transplantation, Maggiore Hospital, IRCCS, Milan, Italy. fabrizi@policlinico.mi.it

RESUMEN / SUMMARY:  - Hepatitis C Virus (HCV) infection is the most important cause of liver disease after renal transplantation (RT). The impact of HCV on patient and graft survival after RT remains controversial; however, the great majority of studies with large size and adequate follow-up have shown the detrimental impact of HCV on long-term patient and graft survival after RT. The use of kidneys from anti-HCV positive donors could help decrease the continuing disparity between the number of patients on the transplant waiting list and the number of patients receiving a transplant each year. Single-center experiences have suggested transplanting kidneys from anti-HCV positive donors only in anti-HCV positive dialysis patients. Such practice has not demonstrated any adverse effect on the short-term patient survival; the waiting times for RT were shortened. A better alternative seems to be a policy of transplanting kidneys from anti-HCV positive donors only in HCV RNA positive recipients. This requires HCV RNA testing of all anti-HCV positive dialysis patients awaiting RT. Matching donors and recipients for HCV genotype has been suggested; however, the assessment of donor HCV genotype is currently hampered by time constraints. Recent evidence based on large data base demonstrated that RT recipients of HCV-positive donors are at independent increased risk of mortality; unadjusted 3-year patient survival was 85% versus 93% (P=0.01) in all recipients of donor HCV-positive and HCV-negative kidneys, respectively. This was observed in all recipient subgroups including elderly and HCV-positive recipients. In the near future, rapid nucleic acid testing (NAT) of donors and recipients will allow the assessment of the HCV viremic status in order to maximize organ use. With appropriate informed consent, use of a renal graft from an HCV positive donor may be offered to an HCV infected recipient. Additional studies are needed to clarify the link between donor HCV-positive kidneys and patient mortality.  N. Ref:: 69

 

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[85]

TÍTULO / TITLE:  - Therapeutic apheresis therapy for ABO-incompatible renal transplantations.

REVISTA / JOURNAL:  - Therap Apher Dial 2003 Dec;7(6):520-8.

AUTORES / AUTHORS:  - Ishida H; Tanabe K; Toma H; Akiba T

INSTITUCIÓN / INSTITUTION:  - Department of Urology, Division of Blood Purification, Tokyo Women’s Medical University, Tokyo, Japan. tgphide@gol.com

RESUMEN / SUMMARY:  - The most important transplantation antigen system for organ transplantation is the ABO blood group system. Crossing the blood barrier is usually not done except in emergency cases such as liver transplantations for fulminant hepatitis. Early experiences of allograft transplantations across the blood barriers were discouraging. In the 1970s, clinical trials were started transplanting kidneys of subgroup A2 into blood group O recipients because the tissues of the A2 subgroup express a lower amount of A antigens compared with subgroup A1. The recipients required no special treatment and received the standard immunosuppressive regimen as used in blood group identical cases. Many early graft loses immediately after transplantations were experienced, but these trials resulted in an excellent graft survival rate. A few centers have adapted the concept of A2 kidneys to non-A recipient transplantations with successful results by reducing anti-A blood type titers prior to transplantations. In the early 1980s, the possibility of bridging the ABO barrier was tested by several groups. A1 and B kidneys from living donors were also successfully transplanted across the blood barrier using quadruple immunosuppressive drugs and splenectomy. Since 1989, the largest number of ABO-incompatible renal transplantations have been performed in Japan because of the limited numbers of cadaveric donors. Approximately 400 cases have been successfully transplanted across the blood barrier at many centers in Japan. Owing to novel immunosuppressive drugs, the ABO-incompatible allografts exhibited a level of function comparable with that of ABO-matched allografts even though anti-A or anti-B antibodies had returned to the circulation of the recipients. In this article, we describe the historical background, the current therapeutic strategies including apheresis therapy for the ABO-incompatible transplantations, and the experiences at our institution.  N. Ref:: 26

 

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[86]

TÍTULO / TITLE:  - Management of hepatitis B after renal transplantation: an update.

REVISTA / JOURNAL:  - J Nephrol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.jnephrol.com/ 

      ●● Cita: Journal of Nephrology: <> 2002 Mar-Apr;15(2):113-22.

AUTORES / AUTHORS:  - Fabrizi F; Lunghi G; Poordad FF; Martin P

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology and Dialysis, Institute of Hygiene and Preventive Medicine, Ospedale Maggiore Policlinico, IRCCS, Milano, Italy. fabrizi@policlinico.mi.it

RESUMEN / SUMMARY:  - Hepatitis B Virus (HBV) infection remains an important cause of liver disease in renal transplant (RT) recipients and the outcome of HBV infected RT recipients is less favorable than that of noninfected RT recipients. The concern about graft loss induced by interferon (IFN) therapy precludes its use; lamivudine, a second-generation analog, has been recently approved for the treatment of hepatitis B and promises to be highly effective in this setting. Several uncontrolled trials have reported a high rate of biochemical (ranging between 80% and 100%) and virological (ranging between 67% and 100%) response in RT recipients, comparable to immunocompetent patients. Lamivudine has an excellent safety profile in RT recipients. However, the emergence of viral mutations leading to resistance has been reported during lamivudine therapy in RT recipients. In addition, numerous issues remain to be clarified about lamivudine use after RT including the management of viral resistance, the role of HBV genotypes in the response to lamivudine, and the duration of therapy and response. The combination of newer nucleoside analogues with lamivudine, analogous to HIV, may further improve the efficacy of antiviral therapy against HBV after RT.  N. Ref:: 85

 

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[87]

TÍTULO / TITLE:  - Polyclonal antibodies induction therapy in kidney transplantation: a single center experience.

REVISTA / JOURNAL:  - Ann Transplant 2002;7(4):46-8.

AUTORES / AUTHORS:  - Malaise J; De Meyer M; Mourad M; Squifflet JP

INSTITUCIÓN / INSTITUTION:  - Department of Renal and Pancreatic Transplantation, Universite Catholique de Louvain Medical School, Brussels, Belgium. Jacques.Malaise@chir.ucl.ac.be  N. Ref:: 18

 

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[88]

TÍTULO / TITLE:  - Use of basiliximab and daclizumab in kidney transplantation.

REVISTA / JOURNAL:  - Prog Transplant 2001 Mar;11(1):33-7; quiz 38-9.

AUTORES / AUTHORS:  - Olyaei AJ; Thi K; deMattos AM; Bennett WM

INSTITUCIÓN / INSTITUTION:  - Oregon Health Sciences University, Portland, Ore., USA.

RESUMEN / SUMMARY:  - Kidney transplantation represents a major medical victory in patients with whom dialysis and medical therapy have failed. To increase survival rates and optimize the use of limited organs, both patient care and immunosuppression therapy must be improved. Reduction in rejection episodes or severity of rejection may ultimately improve long-term allograft survival. Traditional engineered monoclonal antibodies have been associated with severe cytokine release reactions and an increased risk of opportunistic infections. Basiliximab and daclizumab are chimeric and humanized monoclonal antibodies which inhibit thymus-dependent lymphocyte proliferation. Interleukin-2 also affects the proliferation of natural killer cells, macrophages and monocytes, bursa-equivalent lymphocytes, epidermal dendritic cells, and lymphokine-activated killer cells. Interleukin-2 receptor antagonists have been shown to reduce the incidence of acute rejection without increasing the incidence of opportunistic infections or malignancy. Further studies are needed to evaluate the overall effect of these agents on long-term patient and allograft survival.  N. Ref:: 28

 

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[89]

TÍTULO / TITLE:  - RAS blockade in experimental renal transplantation. Benefits and limitations.

REVISTA / JOURNAL:  - Curr Drug Targets Cardiovasc Haematol Disord 2003 Mar;3(1):73-9.

AUTORES / AUTHORS:  - Smit-van Oosten A; Stegeman CA; van Goor H

INSTITUCIÓN / INSTITUTION:  - Department of Pathology and Laboratory Medicine, University Hospital Groningen, The Netherlands. a.smit-van.oosten@med.rug.nl

RESUMEN / SUMMARY:  - In renal transplantation, chronic renal transplant failure (CRTF) is the principal cause of late graft loss. Both immunological and non-immunological factors play a role in the pathogenesis of CRTF. However, CRTF is unresponsive to immunosuppressive therapy. In several kidney diseases, inhibition of the renin-angiotensin system (RAS) has shown to reduce the rate of progression of renal disease more effectively than conventional antihypertensive drugs. Therefore, RAS blockade may be of benefit in the treatment of CRTF. Several short-term studies in human renal transplant recipients showed that RAS blockade had a beneficial effect on renal transplant function, blood pressure and proteinuria. Despite these benefits physicians remain reluctant to use ACE inhibition in these recipients, because of fear of functional decrease in renal perfusion, especially in the setting of renal transplant artery stenosis. To study the long-term effects of RAS blockade we used the established Fisher to Lewis (F-L) model for CRTF, which mirrors the progressive changes seen in humans. Studies in our lab and by others showed that RAS blockade in the F-L model prevents proteinuria, glomerulosclerosis and hypertension. However, when treated for 34 weeks with RAS blockade, renal arteries developed severe intimal hyperplasia. This effect was specific for Fisher rats. Syngrafted Fisher rats treated with ACE inhibition developed intimal hyperplasia, but allografting significantly aggravated it. Fisher rats have a four times higher renal ACE activity, compared with the Lewis rat. This is comparable to the human DD/II genotype differences in ACE activity. Renal transplant patients with the DD genotype may be more vulnerable for vascular changes when treated with RAS blockade.  N. Ref:: 62

 

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[90]

TÍTULO / TITLE:  - Immune profiling: molecular monitoring in renal transplantation.

REVISTA / JOURNAL:  - Front Biosci 2003 Sep 1;8:e444-62.

AUTORES / AUTHORS:  - Hoffmann SC; Pearl JP; Blair PJ; Kirk AD

INSTITUCIÓN / INSTITUTION:  - Transplantation Section, Transplantation and Autoimmunity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20889, USA.

RESUMEN / SUMMARY:  - Molecular techniques have become a mainstay for most biomedical research. In particular, sensitive methods for gene transcript detection and advanced flow cytometry have been crucial in fostering our understanding of the basic mechanisms promoting allosensitization and adaptive immune regulation. These technologies have been validated in vitro, and in pre-clinical settings, and as such their clinical application is now clearly appropriate. It is becoming increasingly clear that these robust techniques hold much promise to better elucidate human transplant biology, and more importantly, guide clinical decision making with mechanistically-based information. This article will discuss our laboratory’s use of several novel technologies, including gene polymorphism analysis, real-time polymerase chain reaction transcript quantification, and multi-color flow cytometry in clinical human renal transplantation. Specific technical methodology will be presented outlining keys for effective clinical application. Clinical correlations will be presented as examples of how these techniques may have clinical relevance. Suggestions for the adaptation of these methods for therapeutic intervention will be given. We propose that clinical transplantation should proceed in close step with modern molecular diagnostics.  N. Ref:: 84

 

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[91]

TÍTULO / TITLE:  - Pathogenesis and molecular mechanisms of chronic allograft nephropathy.

REVISTA / JOURNAL:  - Contrib Nephrol 2003;139:187-204.

AUTORES / AUTHORS:  - Ahsan N; Cheung JY

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology and Transplantation, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, One Robert Wood Johnson Place, MEB 412, New Brunswick, NJ 08903, USA. ahsanna@umdnj.edu  N. Ref:: 92

 

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[92]

TÍTULO / TITLE:  - Abnormal lipid metabolism after renal transplantation.

REVISTA / JOURNAL:  - Ann Transplant 2001;6(1):5-8.

AUTORES / AUTHORS:  - Wanner C; Quaschning T

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, Division of Nephrology, University Hospital Wurzburg, Germany. c.wanner@medizin.uni-wuerzburg.de

RESUMEN / SUMMARY:  - The evidence that lipid disorders in patients following renal transplantation play a major role in the pathogenesis of atherosclerosis and chronic renal allograft rejection is circumstantial. The high rate of clinical vascular disease and cardiovascular complications in renal transplant recipients, the high prevalence of an atherogenic dyslipidemia and the evidence from the statin regression trials in the general population suggest that lipid lowering treatment is beneficial in patients after renal transplantation. In addition, animal models and observational studies in patients have demonstrated correlations between plasma lipid levels and both acute and chronic rejection. Animal transplant models and clinical trials in heart transplant patients also suggest that statin treatment decreases the incidence of chronic rejection. However, the mechanisms behind this protective effect remain unsolved and no conclusive data exist proving that statins directly inhibit the development of chronic rejection. However, sufficient evidence exists to consider the use of these agents in the posttransplant setting for their possible effects on cardiovascular complications.  N. Ref:: 32

 

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[93]

TÍTULO / TITLE:  - Historical overview of the use of cytomegalovirus hyperimmune globulin in organ transplantation.

REVISTA / JOURNAL:  - Transpl Infect Dis 2001;3 Suppl 2:6-13.

AUTORES / AUTHORS:  - Snydman DR

INSTITUCIÓN / INSTITUTION:  - Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Boston, Massachusetts 02111, USA. dsnydman@lifespan.org

RESUMEN / SUMMARY:  - A historical review of the development of cytomegalovirus hyperimmune globulin (CMV-IG, CytoGam) shows its increasing use in solid organ transplant (SOT) recipients, either alone or in combination with ganciclovir. A review of clinical trials of CytoGam in renal transplant recipients shows reductions in CMV-associated syndromes and fungal and parasitic superinfections, and increases in graft survival, while CytoGam prophylaxis trials in orthotopic liver transplant (OLT) recipients have produced reductions in severe CMV-associated disease and invasive fungal disease. A combination of CytoGam plus ganciclovir in OLT recipients has resulted in reductions in CMV hepatitis and infection, and CMV disease and viremia, plus a trend in improved 1- and 2-year survival rates.  N. Ref:: 18

 

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[94]

TÍTULO / TITLE:  - Applications of microarrays to renal transplantation: progress and possibilities.

REVISTA / JOURNAL:  - Front Biosci 2003 Sep 1;8:s913-23.

AUTORES / AUTHORS:  - Chua MS; Mansfield E; Sarwal M

INSTITUCIÓN / INSTITUTION:  - Department of Pediatrics, Stanford University School of Medicine, G320, 300 Pasteur Drive, Stanford, CA 94305, USA.

RESUMEN / SUMMARY:  - By rapidly generating global views of gene expression profiles, microarray technology offers a great advantage over traditional methods of studying gene expression. This technology is gaining rapid and widespread use in many areas of science and medicine because it can be easily adapted to study many experimental questions. This article will review the current applications of microarray technology in the field of renal transplantation, and discuss the potential impact of this technology on transplantation medicine.  N. Ref:: 49

 

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[95]

TÍTULO / TITLE:  - Transmission of viral hepatitis by kidney transplantation: donor evaluation and transplant policies (Part 1: hepatitis B virus).

REVISTA / JOURNAL:  - Transpl Infect Dis 2002 Sep;4(3):124-31.

AUTORES / AUTHORS:  - Natov SN

INSTITUCIÓN / INSTITUTION:  - Tufts University School of Medicine, Tufts-New England Medical Center, Boston, Massachusetts, USA.

RESUMEN / SUMMARY:  - This two-part article discusses serologic testing of prospective donors for viral hepatitis B and C as part of the comprehensive donor evaluation and reviews of the current policies and practices aimed at preventing donor-to-recipient transmission of hepatitis B and C viruses (HBV, HBC). This second part of the review discusses HCV. Organs procured from HCV-infected donors can transmit the virus to their recipients. Because a number of studies have associated infections with HCV with increased morbidity and mortality among renal transplant recipients, it is important to prevent HCV transmission with renal transplantation. The majority of organ procurement organizations (OPOs) perform routine screening of organ donors for antibodies to HCV (anti-HCV). The prevalence of HCV infection among cadaver organ donors, ascertained based on a positive anti-HCV test by ELISA2, varies worldwide between 1.08% and 11.8%. The use of kidneys from donors negative for anti-HCV by ELISA2 carries negligible or no risk of transmitting HCV infection. The use of organs from anti-HCV-positive donors has been restricted to life-saving transplants (heart, liver or lung) by the majority of OPOs worldwide. However, discarding kidneys from all anti-HCV positive donors would lead to unnecessary waste of organs because not all anti-HCV positive donors are infectious. Recently, the policy of unconditional restriction on the use of kidneys from anti-HCV positive donors has been challenged, and transplantation of organs from anti-HCV-positive donors into anti-HCV-positive recipients has been found to be safe. An even better alternative might be a policy of transplanting kidneys from anti-HCV-positive donors only in HCV RNA-positive recipients. However, until more data become available, these two strategies remain experimental treatments.  N. Ref:: 51

 

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[96]

TÍTULO / TITLE:  - Transplanting kidneys from donors with prior hepatitis B infection: one response to the organ shortage.

REVISTA / JOURNAL:  - J Nephrol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.jnephrol.com/ 

      ●● Cita: Journal of Nephrology: <> 2002 Nov-Dec;15(6):605-13.

AUTORES / AUTHORS:  - Fabrizio F; Bunnapradist S; Martin P

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, Dialysis and Transplantation, Maggiore Hospital, Policlinico IRCCS, Milano, Italy. fabrizi@policlinico.mi.it

RESUMEN / SUMMARY:  - While the number of cadaveric organ donors remains relatively stable, the number of patients awaiting transplantation continues to increase, creating a shortage of donor organs. To address this imbalance, there is interest in transplanting organs formerly considered marginal or undesirable. Thus, more organs are currently transplanted from living donors, older donors, hemodynamically unstable donors, non-heart-beating donors and donors with markers of prior hepatitis B virus (HBV) infection. A large number (up to 93.8%) of liver transplant seronegative recipients from anti-HBc antibody positive donors have acquired HBsAg after liver transplantation in the absence of immunoprophylaxis. Based on experience in liver transplantation programs, transmission of HBV from donors without HBsAg but with antibody to HBV core antigen (anti-HBc), although conventionally defined as evidence of resolved infection, can have adverse consequences on both graft and recipient. On the contrary, HBV appears to be in-frequently transmitted from HBsAg negative/anti-HBcAb positive kidney donors: the incidence of de novo HBsAg seropositivity after renal transplantation ranges between 0 and 5.2%. A significantly higher incidence of anti-HBc antibody seroconversion (without developing HBsAg) after renal transplantation with anti-HBc antibody positive donors was seen. However, anti-HBc antibody positive renal allografts should be considered, especially for recipients who have been successfully immunized with HBV vaccine. Prospective long-term studies are in progress to assess the risk of de novo HBV infection (HBsAg seroconversion) in renal transplant recipients who have not been successfully immunized with vaccine against HBV.  N. Ref:: 58

 

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[97]

TÍTULO / TITLE:  - Daclizumab as induction therapy in kidney and simultaneous pancreas-kidney transplantation.

REVISTA / JOURNAL:  - Minerva Urol Nefrol 2003 Mar;55(1):43-56.

AUTORES / AUTHORS:  - Ciancio G; Mattiazzi A; Miller J; Burke GW

INSTITUCIÓN / INSTITUTION:  - Division of Transplantation, Department of Surgery, University of Miami School of Medicine, Miami, FL 33101, USA. gciancio@med.miami.edu

RESUMEN / SUMMARY:  - Acute rejection still remains a major problem in organ transplantation and is a significant risk factor for chronic rejection, and chronic rejection is one of the most important causes of late graft loss. Current new immunosuppressive drugs such as tacrolimus, rapamycin and mycophenolate mofetil have been developed to reduce acute rejection and to improve renal allograft survival. More recently, antihuman antibodies, either monoclonal or polyclonal, have been developed to use for induction therapy at the time of transplantation or to treat rejection. Daclizumab, a new engineered human immunoglobulin monoclonal antibody to the interleukin-2 receptor a-subunit was approved to prevent acute rejection after solid organ transplantation. Data from clinical trials show daclizumab to be well tolerated in solid organ transplantation. It does not increase the incidence of infection, including cytomegalovirus infection.  N. Ref:: 72

 

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[98]

TÍTULO / TITLE:  - Update on drug sieving coefficients and dosing adjustments during continuous renal replacement therapies.

REVISTA / JOURNAL:  - Contrib Nephrol 2001;(132):349-53.

AUTORES / AUTHORS:  - Golper TA

INSTITUCIÓN / INSTITUTION:  - Vanderbilt University Medical Center, Nashville, Tenn., USA.  N. Ref:: 17

 

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[99]

TÍTULO / TITLE:  - Metabolic aspects of tacrolimus in renal transplantation. Consequences for the choice of an immunosuppressive regimen and for the management of post-transplant diabetes mellitus.

REVISTA / JOURNAL:  - Minerva Urol Nefrol 2003 Mar;55(1):33-42.

AUTORES / AUTHORS:  - van Duijnhoven EM; Boots JM; Christiaans MH; van Hooff JP

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine,University Hospital Maastricht, Maastricht, The Netherlands. evd@sint.azm.nl

RESUMEN / SUMMARY:  - The occurrence of post-transplant diabetes mellitus (PTDM) is an important complication after renal transplantation associated with an increased risk of chronic transplant dysfunction and of cardiovascular morbidity and mortality. Both tacrolimus and cyclosporine have been associated with PTDM. In the initial studies, PTDM seemed to occur more often in tacrolimus treated patients than in cyclosporine treated patients. The mechanism by which tacrolimus could cause PTDM was unknown and the relative roles of tacrolimus and corticosteroids, which are often prescribed concomitantly with tacrolimus, were unknown. In several studies we used fasting glucose and insulin levels to assess (peripheral) insulin resistance, and intravenous glucose tolerance tests to assess insulin secretion by the pancreatic b-cells in response to a stimulus (glucose load). Thus, we evaluated the mechanism by which tacrolimus causes glucose metabolic disorders, risk factors for glucose metabolic disorders during tacrolimus treatment, the relative roles of corticosteroids and tacrolimus trough levels in glucose metabolic disorders, and also differences in glucose metabolism between patients using tacrolimus versus patients using cyclosporine. Based on the results of these studies and the available literature, the consequences for the choice of a primary immunosuppressive agent and guidelines for the treatment of PTDM during tacrolimus-based immunosuppression are discussed.  N. Ref:: 40

 

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[100]

TÍTULO / TITLE:  - Renin-angiotensin system in chronic renal allograft dysfunction.

REVISTA / JOURNAL:  - Contrib Nephrol 2001;(135):222-34.

AUTORES / AUTHORS:  - Shihab FS

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, University of Utah School of Medicine, Salt Lake City, Utah, USA. Fuad.Shihab@hsc.utah.edu  N. Ref:: 44

 

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[101]

TÍTULO / TITLE:  - Induction immunotherapy with IL-2Ra monoclonal antibody in kidney transplantation.

REVISTA / JOURNAL:  - Minerva Urol Nefrol 2003 Mar;55(1):67-79.

AUTORES / AUTHORS:  - Ahsan N

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology and Transplantation, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick, NJ 08903, USA. ahsanna@umdnj.edu

RESUMEN / SUMMARY:  - The development of new immunosuppressive agents is designed to reduce the incidence and severity of early acute post-transplant rejection. One potential target for more specific immunosuppressive therapy with monoclonal antibodies is the high affinity a chain of interleukin-2 receptors (IL-2Ra). Clinical investigation of murine IL-2Ra monoclonal antibodies (IL-2Ra mAb) in renal transplantation has indicated that a complete blockade of IL-2Ra during the critical first post-transplant months allows effective immunoprophylaxis, especially in the early post-transplant period. Efficacy of these agents, however, is hampered by their short disposition half-lives in humans and their immunogenicity in the form of neutralizing human antimouse antibodies. These inherent problems can be partially overcome by chi-meric, hyper-chimeric (humanized) products and multiple dose regimens. Both IL-2Ra mAbs: daclizumab (humanized) and basiliximab (chimeric) currently approved for clinical use have been found to reduce the frequency of acute rejections in renal transplant recipients without an apparent increase in short-term toxicities. In most transplant centers where these agents are utilized, they are being routinely administered as induction immunoprophylaxis in recommended multiple dose regimens to recipients of solid organ transplants. Others have restricted their use to certain high-risk patients such as those undergoing multi-organ transplantation, recipients with high panel-reactive antibodies, African-Americans, patients at risk for developing delayed graft function (DGF), and children. Recently some investigators have successfully administered these antibodies co-administered with newer immunosuppressive agents in limited dose protocols thus developing cost effective and simplified regimens. Therefore, in the absence of a favorable long-term efficacy, it is likely that these agents will be administered in limited dose protocols along with one of the modulators of IL-2, i.e. calcineurin inhibitors (CNI), to a selected group of patients in whom additional immunosuppression in the early post-transplantation period is desirable.  N. Ref:: 59

 

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[102]

TÍTULO / TITLE:  - New monoclonal antibodies in renal transplantation.

REVISTA / JOURNAL:  - Minerva Urol Nefrol 2003 Mar;55(1):57-66.

AUTORES / AUTHORS:  - Vincenti F

INSTITUCIÓN / INSTITUTION:  - Kidney Transplant Service, University of California, San Francisco, CA 94143-0780, USA. vincentif@surgery.ucsf.edu

RESUMEN / SUMMARY:  - A decade of spectacular innovation in maintenance immunosuppression drugs has resulted in dramatic reductions in acute rejection and improvement in short and long term outcome after renal transplantation. However the new drugs continue to lack specificity, many require frequent therapeutic drug monitoring and all are associated with acute and chronic toxicities. The new biologic agents, monoclonal antibodies (chimeric, humanized, and fully human) and receptor-fusion proteins, lack immunogenicity, have long half-life and prolonged biologic effects, require intermittent administration and have minimal toxicity. The specificity and selectively of the targets of the new biologic agents render them less toxic than the oral maintenance drugs and thus could possibly replace the maintenance drugs most associated with long-term toxicity such as the corticosteroids and the calcineurin inhibitors. The recently introduced anti-interleukin 2 receptor (IL-2R) monoclonal antibodies (mAbs) are the prototype of future biologic agents; selective, safe, and inducing prolonged biologic effects. The IL-2R mAbs have been used with a variety of maintenance immunosuppression regimens double therapy with cyclosporine and prednisone, triple therapy with cyclosporine, azathioprine and prednisone and with newer regimens such as cyclosporine or tacrolimus, mycophenolate mofetil (MMF) and prednisone, and most recently with sirolimus, MMF and prednisone. The major thrust of the new biologics in clinical development is to block the co-stimulatory pathway. The first attempt at blockade of the CD40-CD154 with anti-CD154 mAbs was disappointing. Anti-CD 154 therapy was associated with thromboembolic events and acute rejection. Attempts at blocking the CD28-B7s (CD80-CD86) pathway are currently underway with the receptor fusion protein, LEA29Y a second generation CTL4Aig, and humanized mAbs to CD 80 and CD86. LFA1, an adhesion molecule that also participates in the co-stimulatory pathway, has also been targeted with a mAb that binds to the CD11a chain of LFA1. Efalizumab, a humanized anti-CD11a mAb, was shown in a phase I trial to be potentially effective in renal transplantation. A humanized anti-CD45 RB mAb is currently in pre-clinical studies and will likely be tested in a phase I trial of renal transplantation within 1 year. While excellent results with anti-CD45 RB mAbs have been published in experimental transplantation, the mechanism of action of anti-CD45 RB mAbs remains to be determined. Several antibodies that are currently approved for non-transplant indications are currently used in single center clinical trials in renal transplantation including Campath 1 H, a humanized anti-CD52 mAb, Rituxamab, an anti-CD20 chimeric mAb, and Infliximab an anti-TNFa chimeric mAb. In addition, several humanized mutagenized anti-CD3 mAbs, huOKT3g1, aglycosyl CD3 and HuM291 have been used in limited trials in renal transplantation but have yet to have a formal clinical development. Humanized mAbs and receptor fusion proteins offer the potential of providing renal transplant recipients with a novel algorithm for immunosuppression that relies on chronic intermittent intravenous administration of safe, non-toxic agents replacing oral drug therapy maintenance.  N. Ref:: 50

 

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[103]

TÍTULO / TITLE:  - The next generation of medications for kidney transplant patients.

REVISTA / JOURNAL:  - Crit Care Nurs Clin North Am 2002 Mar;14(1):99-109.

AUTORES / AUTHORS:  - Sims TW; Good EW

INSTITUCIÓN / INSTITUTION:  - Digestive Health Center of Excellence and Surgical Services, University of Virginia Health System, Charlottesville 22906, USA. tws4m@virginia.edu

RESUMEN / SUMMARY:  - Transplant pharmacotherapy evolves as new agents are investigated and approved for use. Clinical immunosuppression has been plagued with maintaining a balance between rejection of the transplanted organ and complications of over-immunosuppression, including infection and malignancy. Clinicians must understand current immunosuppressive regimens and their associated effects when caring for transplant patients. While all transplant patients receive some form of immunosuppressive therapy, the combinations and choices increase as new drugs are developed. In the critical and acute care settings, newly transplanted patients will likely receive induction therapy. The goal of induction therapy is to increase long-term patient and allograft survival while preventing or reducing rejection episodes. Several agents are available for induction therapy, and each transplant center designs its own protocol. The foundation for maintenance therapy rests on the combining immunosuppressives to prevent rejection through a variety of pathways. An understanding of the mechanism of action and additive effects of a drug allows practitioners to optimize therapy while decreasing adverse effects. Immunosuppressive therapy offers potential for reducing detrimental patient outcomes and improving allograft survival. It is well established that repeated rejection episodes correlate with poor long-term graft survival. Challenges facing researchers and clinicians focus on improved patient outcomes and options to address financial constraints of transplantation.  N. Ref:: 33

 

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[104]

TÍTULO / TITLE:  - Leptin and transplantation: pieces are still missing in the puzzle.

REVISTA / JOURNAL:  - Isr Med Assoc J 2002 Mar;4(3):207-8.

AUTORES / AUTHORS:  - Modan-Moses D; Paret G  N. Ref:: 24

 

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[105]

TÍTULO / TITLE:  - Polyoma virus in renal transplant recipients.

REVISTA / JOURNAL:  - Nephrol Nurs J 2002 Jun;29(3):247-50; quiz 251-2.

AUTORES / AUTHORS:  - Weiskittel PD

INSTITUCIÓN / INSTITUTION:  - University Hospital, Cincinnati, OH, USA.

RESUMEN / SUMMARY:  - Infection and rejection have been the most critical complications following renal transplantation. Rejection rates have decreased recently with the advent of new and more powerful immunosuppressive agents. However, infection continues to be a serious complication. The use of broad-spectrum antibiotics and the development of antiviral agents have provided effective tools to combat the infectious processes traditionally seen in renal transplant recipients. Recently, a new viral illness has been identified in this population. Polyoma virus infection has been identified as the cause of allograft dysfunction and graft loss. This paper reviews the current prevalence and outcome of renal transplant patients infected with polyoma virus.  N. Ref:: 16

 

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