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Revisiones-Ciencias Básicas-Molecular, Bioquímica, Metabolismo & Genética *** Reviews-Basic Sciences-Molecular, Biochemistry, Metabolism & Genetics

 

INMUNOSUPRESIÓN *** IMMUNOSUPPRESSION

(Conceptos / Keywords: Immunosuppression; Immunosuppressive ag.; Transpl. immunol.; GVH; Antirejection therapy; Lymphocyte depletion; Transpl. conditioning; etc).

 

Enero / January 2001 --- Marzo / March 2004

 

La biblioteca del conocimiento biomédico© es un servicio GRATUITO. Toda la información ha sido obtenida de fuentes públicas, de portales de internet que no requieren registro alguno para su uso, que no requieren estar de acuerdo con sus Términos de uso, que son de libre acceso a todo el mundo, y son a su vez gratuitos. La biblioteca (revisiones, guías, protocolos, medicina basada en la evidencia, etc...) se recopila en base a una patente que permite a Effiloop la catalogación de los artículos por campos de interés así como por el orden de su importancia (se proveen las primeras 200 revisiones). Toda la informacion se ampara en las leyes de libre pensamiento-expresión, y de uso justo. Este documento sólo contiene artículos escritos en Castellano y/o Inglés.

 

The biomedical library© is a FREE service. All the information has been obtained from public sources, from web sites that do not require registration for their use, that do not require an agreement with their Terms of use, that provide free access for all, and are free of charge. The library (reviews, guides, protocols, medicine based medicine, etc) is arranged according to a patent that warrants Effiloop to catalogue the articles by fields of interest as well as to sort articles by true relevance (the first 200 reviews are provided). All the information is provided according to the freedom of speech and fair use laws. Only articles written in Spanish and/or English are included.

Enlace / Link

 

[1]

TÍTULO / TITLE:  - Hepcidin: a putative iron-regulatory hormone relevant to hereditary hemochromatosis and the anemia of chronic disease.

REVISTA / JOURNAL:  - Proc Natl Acad Sci U S A. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.pnas.org/ 

      ●● Cita: Proc Natl Acad Sci USA (PNAS): <> 2001 Jul 17;98(15):8160-2.

      ●● Enlace al texto completo (gratuito o de pago) 1073/pnas.161296298

AUTORES / AUTHORS:  - Fleming RE; Sly WS

INSTITUCIÓN / INSTITUTION:  - Department of Pediatrics, Saint Louis University School of Medicine, St. Louis, MO 63014, USA.  N. Ref:: 30

 

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[2]

TÍTULO / TITLE:  - Beta lactam monotherapy versus beta lactam-aminoglycoside combination therapy for sepsis in immunocompetent patients: systematic review and meta-analysis of randomised trials.

REVISTA / JOURNAL:  - British Medical J (BMJ). Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://bmj.com/search.dtl 

      ●● Cita: British Medical J. (BMJ): <> 2004 Mar 20;328(7441):668. Epub 2004 Mar 2.

      ●● Enlace al texto completo (gratuito o de pago) 1136/bmj.38028.520995.63

AUTORES / AUTHORS:  - Paul M; Benuri-Silbiger I; Soares-Weiser K; Leibovici L

INSTITUCIÓN / INSTITUTION:  - Department of Medicine E and Infectious Diseases Unit, Rabin Medical Centre, Beilinson Campus, Petah-Tikva 49100, Israel. mica@zahav.net.il

RESUMEN / SUMMARY:  - OBJECTIVE: To compare beta lactam monotherapy with beta lactam-aminoglycoside combination therapy for severe infections. DATA SOURCES: Medline, Embase, Lilacs, Cochrane Library, and conference proceedings, to 2003; references of included studies; contact with all authors. No restrictions, such as language, year of publication, or publication status. STUDY SELECTION: All randomised trials of beta lactam monotherapy compared with beta lactam-aminoglycoside combination therapy for patients without neutropenia who fulfilled criteria for sepsis. DATA SELECTION: Two reviewers independently applied selection criteria, performed quality assessment, and extracted the data. The primary outcome assessed was all cause fatality by intention to treat. Relative risks were pooled with the random effect model (relative risk < 1 favours monotherapy). RESULTS: 64 trials with 7586 patients were included. There was no difference in all cause fatality (relative risk 0.90, 95% confidence interval 0.77 to 1.06). 12 studies compared the same beta lactam (1.02, 0.76 to 1.38), and 31 studies compared different beta lactams (0.85, 0.69 to 1.05). Clinical failure was more common with combination treatment overall (0.87, 0.78 to 0.97) and among studies comparing different beta lactams (0.76, 0.68 to 0.86). There was no advantage to combination therapy among patients with Gram negative infections (1835 patients) or Pseudomonas aeruginosa infections (426 patients). There was no difference in the rate of development of resistance. Nephrotoxicity was significantly more common with combination therapy (0.36, 0.28 to 0.47). Heterogeneity was not significant for these comparisons. CONCLUSIONS: In the treatment of sepsis the addition of an aminoglycoside to beta lactams should be discouraged. Fatality remains unchanged, while the risk for adverse events is increased.  N. Ref:: 26

 

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[3]

TÍTULO / TITLE:  - Immunopathogenesis and immunotherapy in AIDS virus infections.

REVISTA / JOURNAL:  - Nat Med 2003 Jul;9(7):861-6.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nm0703-861

AUTORES / AUTHORS:  - Letvin NL; Walker BD

INSTITUCIÓN / INSTITUTION:  - Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.

RESUMEN / SUMMARY:  - The heterogeneity of HIV and the different human leukocyte antigen (HLA) backgrounds of infected individuals have posed challenges to understanding the pathogenesis of HIV infection. But continuing advances in our knowledge of the role of immune responses in controlling HIV viremia should help to define goals for immune-based therapies and vaccine strategies against AIDS.  N. Ref:: 106

 

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[4]

TÍTULO / TITLE:  - Integration of growth factor and nutrient signaling: implications for cancer biology.

REVISTA / JOURNAL:  - Mol Cell 2003 Aug;12(2):271-80.

AUTORES / AUTHORS:  - Shamji AF; Nghiem P; Schreiber SL

INSTITUCIÓN / INSTITUTION:  - Harvard Biophysics Program, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.

RESUMEN / SUMMARY:  - Signaling networks that promote cell growth are frequently dysregulated in cancer. One regulatory network, which converges on effectors such as 4EBP1 and S6K1, leads to growth by promoting protein synthesis. Here, we discuss how this network is regulated by both extracellular signals, such as growth factors, and intracellular signals, such as nutrients. We discuss how mutations amplifying either type of signal can lead to tumor formation. In particular, we focus on the recent discovery that a tumor suppressor complex whose function is lost in tuberous sclerosis patients regulates the nutrient signal carried by the critical signaling protein TOR to the effectors 4EBP1 and S6K1. Finally, we describe how the small molecule rapamycin, which inhibits TOR and thereby the activation of these effectors, could be useful to treat tumors that have become dependent upon this pathway for growth.  N. Ref:: 80

 

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[5]

TÍTULO / TITLE:  - Defying death—HIV mutation to evade cytotoxic T lymphocytes.

REVISTA / JOURNAL:  - N Engl J Med. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://content.nejm.org/ 

      ●● Cita: New England J Medicine (NEJM): <> 2002 Oct 10;347(15):1203-4.

      ●● Enlace al texto completo (gratuito o de pago) 1056/NEJMcibr022067

AUTORES / AUTHORS:  - Lieberman J

INSTITUCIÓN / INSTITUTION:  - Center for Blood Research, Boston, MA 02115, USA.  N. Ref:: 5

 

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[6]

TÍTULO / TITLE:  - Genetic and functional relationships between MHC and NK receptor genes.

REVISTA / JOURNAL:  - Immunity 2001 Sep;15(3):363-74.

AUTORES / AUTHORS:  - Trowsdale J

INSTITUCIÓN / INSTITUTION:  - Immunology Division, Pathology Department, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom.

RESUMEN / SUMMARY:  - HLA class I and NK receptors are encoded within dense clusters of immune loci. The MHC, at 6p21.3, and the complex containing the KIR loci, at 19q13.4, both feature variation in the number of genes, as well as sequence polymorphism. In addition to T cell receptors, several variable class I-related molecules interact with polymorphic NK receptors. Some of the lectin-related NK receptor genes, at 12p13.1, also have ligands belonging to the extended class I family. The expanding clusters of class I-related sequences and their receptors, some of which evolved recently, reveal further complexity in immune recognition of disease.  N. Ref:: 85

 

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[7]

TÍTULO / TITLE:  - Impact of shared epitope genotype and ethnicity on erosive disease: a meta-analysis of 3,240 rheumatoid arthritis patients.

REVISTA / JOURNAL:  - Arthritis Rheum 2004 Feb;50(2):400-12.

      ●● Enlace al texto completo (gratuito o de pago) 1002/art.20006

AUTORES / AUTHORS:  - Gorman JD; Lum RF; Chen JJ; Suarez-Almazor ME; Thomson G; Criswell LA

INSTITUCIÓN / INSTITUTION:  - University of California, San Francisco, CA 94143-0500, USA. lac@itsa.ucsf.edu

RESUMEN / SUMMARY:  - OBJECTIVE: The strongest known genetic association in rheumatoid arthritis (RA) is with HLA-DRB1 alleles that share a similar amino acid sequence, termed the shared epitope (SE). Although many studies have examined the association of the SE with disease severity, the results have been inconsistent, which may reflect the relatively small sample sizes or ethnic differences. The aim of this study was to assess the association of HLA-DRB1 SE alleles and genotype with the development of bony erosions in RA by meta-analysis. METHODS: We identified English-language articles published between January 1, 1987 and June 1, 1999 through Medline, EMBase, and manual searches of 6 relevant journals. Included were studies in which molecular typing of HLA-DRB1 alleles was performed and in which the presence or absence of bony erosions was reported. Data were extracted from the studies, and erosions were coded as present or absent. Authors were contacted for missing information and data on individual patients. RESULTS: A total of 29 studies and 3,240 patients were available for analysis. The summary odds ratios (ORs), when all patients were evaluated as a single group, demonstrated a significant association of the presence of the SE (2 or 1 versus 0 SE alleles) with erosions (OR 2.0; 95% confidence interval [95% CI] 1.8-2.2), although significant heterogeneity was present (P = 0.002). Subgroup analyses demonstrated the important influence of ethnic background. For example, no association of the SE with erosions was demonstrated in Greeks (OR 0.8 [95% CI 0.2-1.5]). In contrast, there was a striking dose-dependent relationship in southern European Caucasians and Asians, with ORs as high as 6.2 and 5.4, respectively, in patients with 2 SE alleles. Although our ability to assess the relationship between SE genotype and erosions was limited, particular importance of the DRB1*0401 SE allele was suggested in an analysis restricted to northern European Caucasians. CONCLUSION: The SE is associated with the development of erosive disease in many ethnic groups; however, striking exceptions exist. These variations may be due to allele differences between populations, such as the frequency of DRB1*0401 among different ethnic groups. Further study to better understand the genetic and environmental differences between these populations may provide insight into mechanisms that influence the clinical expression of RA.

 

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[8]

TÍTULO / TITLE:  - Routes to transplant tolerance versus rejection; the role of cytokines.

REVISTA / JOURNAL:  - Immunity 2004 Feb;20(2):121-31.

AUTORES / AUTHORS:  - Walsh PT; Strom TB; Turka LA

INSTITUCIÓN / INSTITUTION:  - University of Pennsylvania, 700 Clinical Research Building, 415 Curie Boulevard, Philadelphia, PA 19104, USA.

RESUMEN / SUMMARY:  - The alloimmune response can be divided into specific junctures where critical decisions between tolerance and immunity are made which define the outcome of the transplant. At these “decision nodes” various cytokines direct alloresponsive T cells to develop either a proinflammatory response aimed at graft destruction or an immunoregulatory response facilitating graft acceptance. This review will focus on the role of these cytokines in influencing the progression of an alloimmune response leading ultimately to either allograft survival or rejection.  N. Ref:: 97

 

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[9]

TÍTULO / TITLE:  - Microchimerism: an investigative frontier in autoimmunity and transplantation.

REVISTA / JOURNAL:  - JAMA. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://jama.ama-assn.org/search.dtl 

      ●● Cita: JAMA: <> 2004 Mar 3;291(9):1127-31.

      ●● Enlace al texto completo (gratuito o de pago) 1001/jama.291.9.1127

AUTORES / AUTHORS:  - Adams KM; Nelson JL

INSTITUCIÓN / INSTITUTION:  - Program in Human Immunogenetics, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.

RESUMEN / SUMMARY:  - Recent studies indicate cells transfer between fetus and mother during pregnancy and can persist in both decades later. The presence within one individual of a small population of cells from another genetically distinct individual is referred to as microchimerism. Naturally acquired microchimerism has recently been investigated in autoimmune diseases, including scleroderma, thyroiditis, primary biliary cirrhosis, Sjogren syndrome, systemic lupus, dermatomyositis, and neonatal lupus. Iatrogenic chimerism has been investigated in transplantation and following blood transfusion. Considering findings of naturally acquired microchimerism along with iatrogenic microchimerism suggests microchimerism can have detrimental and/or beneficial effects in both settings. Recent identification of tissue-specific microchimerism either from naturally acquired or iatrogenic microchimerism (eg, cardiac myocytes) raises the possibility that microchimerism can be a target of autoimmunity or alternatively contribute to tissue repair. Advances in this new frontier of research with varied and numerous implications for human health are summarized.  N. Ref:: 26

 

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[10]

TÍTULO / TITLE:  - The allogeneic response and tumor immunity.

REVISTA / JOURNAL:  - Nat Med 2001 Jun;7(6):649-52.

      ●● Enlace al texto completo (gratuito o de pago) 1038/89008

AUTORES / AUTHORS:  - Fabre JW

INSTITUCIÓN / INSTITUTION:  - Department of Clinical Sciences, Institute of Liver Studies Guy’s, King’s and St Thomas’ School of Medicine, London, UK. john.fabre@kcl.ac.uk

RESUMEN / SUMMARY:  - The strong allogeneic response to donor MHC molecules in transplantation and the weak response to tumor antigens represent two important and divergent but potentially interactive immune responses. A patient’s response to allogeneic MHC molecules might promote an effective T-cell response to self MHC-restricted tumor peptides and the possibilities for this are discussed here. These allogeneic responses might successfully be harnessed to promote the immune eradication of metastatic cancer.  N. Ref:: 45

 

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[11]

TÍTULO / TITLE:  - Lack of association of the HLA-DRB1 shared epitope with rheumatoid nodules: an individual patient data meta-analysis of 3,272 Caucasian patients with rheumatoid arthritis.

REVISTA / JOURNAL:  - Arthritis Rheum 2004 Mar;50(3):753-62.

      ●● Enlace al texto completo (gratuito o de pago) 1002/art.20119

AUTORES / AUTHORS:  - Gorman JD; David-Vaudey E; Pai M; Lum RF; Criswell LA

INSTITUCIÓN / INSTITUTION:  - University of California, San Francisco, and School of Public Health, University of California, Berkeley.

RESUMEN / SUMMARY:  - OBJECTIVE: The objective of this individual patient data (IPD) meta-analysis was to examine the relationship of rheumatoid nodules to the HLA-DRB1 shared epitope (SE) and to individual SE genotypes. METHODS: English-language studies that enrolled adult non-Hispanic Caucasian patients with rheumatoid arthritis (RA) were identified by searches of Medline and Embase, and by manual searches of medical journals. All authors were contacted for IPD. Meta-analysis was performed to assess the association of SE presence, dose, and genotype with rheumatoid nodules. Meta-analyses adjusted for disease duration and cumulative meta-analyses were also performed to assess the influence of RA duration and year of study publication on the results. RESULTS: A total of 24 studies and 3,272 patients were available for analysis. IPD were obtained for 22 of the studies. There was a nonsignificant association between the presence of the SE (i.e., 1 or 2 alleles versus 0 alleles) and rheumatoid nodules (summary odds ratio [OR] 1.3, 95% confidence interval [95% CI] 0.97-1.6). Analysis by SE genotype, however, demonstrated a weak relationship with inheritance of a single DRB1*0401 SE allele (OR 1.4, 95% CI 1.1-1.8). No other genotypes achieved statistical significance in the adjusted or unadjusted analyses. CONCLUSION: The presence of the HLA-DRB1 SE does not appear to significantly increase the risk of rheumatoid nodules among Caucasian patients with RA. Analysis by DRB1 SE genotype was uninformative, suggesting only a potential (and at most modest) role of the DRB1*0401 SE allele. Results from this IPD meta-analysis implicate other genetic, stochastic, and/or environmental factors in the susceptibility to rheumatoid nodules.

 

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[12]

TÍTULO / TITLE:  - Genetic control of MHC class II expression.

REVISTA / JOURNAL:  - Cell 2002 Apr;109 Suppl:S21-33.

AUTORES / AUTHORS:  - Ting JP; Trowsdale J

INSTITUCIÓN / INSTITUTION:  - Department of Microbiology and Immunology and The Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA. panyun@med.unc.edu

RESUMEN / SUMMARY:  - The presentation of peptides to T cells by MHC class II molecules is of critical importance in specific recognition by the immune system. Expression of class II molecules is exquisitely controlled at the transcriptional level. A large set of proteins interact with the promoters of class II genes. The most important of these is CIITA, a master controller that orchestrates expression but does not bind directly to the promoter. The transcriptosome complex formed at class II promoters is a model for induction of gene expression.  N. Ref:: 108

 

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[13]

TÍTULO / TITLE:  - Interferon-gamma reduces interleukin-4- and interleukin-13-augmented transforming growth factor-beta2 production in human bronchial epithelial cells by targeting Smads.

REVISTA / JOURNAL:  - Chest. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.chestjournal.org/ 

      ●● Cita: Chest: <> 2003 Mar;123(3 Suppl):372S-3S.

AUTORES / AUTHORS:  - Wen FQ; Liu XD; Terasaki Y; Fang QH; Kobayashi T; Abe S; Rennard SI

INSTITUCIÓN / INSTITUTION:  - Pulmonary and Critical Care Medicine Section, University of Nebraska Medical Center, Omaha, NE 68198-5125, USA.  N. Ref:: 0

 

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[14]

TÍTULO / TITLE:  - When the lymphocyte loses its clothes.

REVISTA / JOURNAL:  - Immunity 2003 Apr;18(4):453-7.

AUTORES / AUTHORS:  - Nekrep N; Fontes JD; Geyer M; Peterlin BM

INSTITUCIÓN / INSTITUTION:  - Institute of Biochemistry, Medical Faculty of the University of Ljubljana, Slovenia.

RESUMEN / SUMMARY:  - The type II bare lymphocyte syndrome (BLS) or major histocompatibility complex class II (MHCII) deficiency is a severe combined immunodeficiency (SCID) that is characterized by the absence of constitutive and inducible expression of MHCII determinants on immune cells. Four complementation groups of BLS have been defined, and they result from mutations in DNA-bound activators and the coactivator for MHCII transcription. Recently, all complementation groups of BLS patients have been accounted for. Studies of the syndrome and specific mutations reveal important lessons for the genetics of the immune response.  N. Ref:: 35

 

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[15]

TÍTULO / TITLE:  - Ex vivo selection of recipient-type alloantigen-specific CD4(+)CD25(+) immunoregulatory T cells for the control of graft-versus-host disease after allogeneic hematopoietic stem-cell transplantation.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 15;77(1 Suppl):S32-4.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000106470.07410.CA

AUTORES / AUTHORS:  - Trenado A; Fisson S; Braunberger E; Klatzmann D; Salomon BL; Cohen JL

INSTITUCIÓN / INSTITUTION:  - Biologie et Therapeutique des Pathologies Immunitaires, Hopital Pitie-Salpetriere, Paris, France.

RESUMEN / SUMMARY:  - Allogeneic hematopoietic stem-cell transplantation (HSCT) is the treatment of choice for many malignant and nonmalignant hematologic disorders. Donor T cells present in the hematopoietic stem-cell transplant improve engraftment and immune reconstitution and contribute to the graft-versus-leukemia effect, but are also responsible for the life-threatening graft-versus-host disease (GVHD). CD4(+)CD25(+) immunoregulatory T cells, which play a pivotal role in preventing organ-specific diseases, can also modulate GVHD if administered in equal numbers of T cells at the time of grafting. In this article, the authors describe a procedure of ex vivo selection and expansion of regulatory T cells specific for recipient-type alloantigens. These expanded regulatory T cells controlled GVHD. Their therapeutic use in HSCT should allow specific suppression of the activation of donor alloreactive T cells involved in GVHD while preserving the beneficial effects of other T cells.  N. Ref:: 27

 

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[16]

TÍTULO / TITLE:  - Hemochromatosis gene modifies course of hepatitis C viral infection.

REVISTA / JOURNAL:  - Gastroenterology 2003 May;124(5):1509-23.

AUTORES / AUTHORS:  - Pietrangelo A

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine, Centre for Hemochromatosis and Metabolic Liver Diseases, University of Modena and Reggio Emilia, Modeno, Italy. antonello@unimore.it  N. Ref:: 161

 

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[17]

TÍTULO / TITLE:  - T cell receptor-MHC interactions up close.

REVISTA / JOURNAL:  - Cell 2001 Jan 12;104(1):1-4.

AUTORES / AUTHORS:  - Hennecke J; Wiley DC

INSTITUCIÓN / INSTITUTION:  - Department of Molecular and Cellular Biology, Harvard University and Howard Hughes Medical Institute, Cambridge, MA 02138, USA. hennecke@crystal.harvard.edu  N. Ref:: 18

 

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[18]

- Castellano -

TÍTULO / TITLE:La ruta de senalizacion CA++/calcineurina/NFAT en activacion endotelial y angiogenesis: efectos de la ciclosporina A. CA++/ calcineurin/NFAT signaling in endothelial activation and angiogenesis: effects od cyclosporin A .

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2003;23 Suppl 3:44-8.

AUTORES / AUTHORS:  - Quesada AJ; Redondo JM

INSTITUCIÓN / INSTITUTION:  - Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid y Centro Nacional de Investigaciones Cardiovasculares (CNIC), Sinesio Delgado, 4 28049 Cantoblanco, Madrid. jmredondo@cbm.uam.es  N. Ref:: 31

 

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[19]

TÍTULO / TITLE:  - Disease modifying therapies in multiple sclerosis: report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurology and the MS Council for Clinical Practice Guidelines.

REVISTA / JOURNAL:  - Neurology 2002 Jan 22;58(2):169-78.

AUTORES / AUTHORS:  - Goodin DS; Frohman EM; Garmany GP Jr; Halper J; Likosky WH; Lublin FD; Silberberg DH; Stuart WH; van den Noort S

 

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[20]

TÍTULO / TITLE:  - Identification of TOR signaling complexes: more TORC for the cell growth engine.

REVISTA / JOURNAL:  - Cell 2002 Oct 4;111(1):9-12.

AUTORES / AUTHORS:  - Abraham RT

INSTITUCIÓN / INSTITUTION:  - Program in Signal Transduction Research, Cancer Research Center, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA. abraham@burnham.org

RESUMEN / SUMMARY:  - The Target of Rapamycin (TOR) proteins function in signaling pathways that promote protein synthesis and cell growth. In yeast, TOR signaling is regulated by nutrient availability, whereas in metazoan cells TOR activities may be controlled by both nutrients and growth factors. The recent identification of novel TOR-interacting proteins has provided crucial insights into TOR regulation and function.  N. Ref:: 20

 

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[21]

TÍTULO / TITLE:  - Immune tolerance after long-term enzyme-replacement therapy among patients who have mucopolysaccharidosis I.

REVISTA / JOURNAL:  - Lancet 2003 May 10;361(9369):1608-13.

AUTORES / AUTHORS:  - Kakavanos R; Turner CT; Hopwood JJ; Kakkis ED; Brooks DA

INSTITUCIÓN / INSTITUTION:  - Lysosomal Diseases Research Unit, Department of Chemical Pathology, Women’s and Children’s Hospital, North Adelaide, South Australia, Australia

RESUMEN / SUMMARY:  - BACKGROUND: Enzyme-replacement therapy has been assessed as a treatment for patients who have mucopolysaccharidosis I (alpha-L-iduronidase deficiency). We aimed to investigate the humoral immune response to recombinant human alpha-L-iduronidase among these patients. METHODS: We characterised the antibody titres and specific linear sequence epitope reactivity of serum antibodies to alpha-L-iduronidase for ten patients with mucopolysaccharidosis I, at the start of treatment and after 6, 12, 26, 52, and 104 weeks. We compared the values for patients’ samples with those for samples from normal human controls. FINDINGS: Before enzyme-replacement therapy, all patients had low serum antibody titres to recombinant human alpha-L-iduronidase that were within the control range. Five of the ten patients produced higher-than-normal titres of antibody to the replacement protein during the treatment course (serum antibody titres 130000-500000 and high-affinity epitope reactivity). However, by week 26, antibody reactivity was reduced, and by week 104 all patients had low antibody titres and only low-affinity epitope reactivity. Patients who had mucopolysaccharidosis I with antibody titres within the normal range at 6-12 weeks did not subsequently develop immune responses. INTERPRETATION: After 2 years of treatment, patients who initially had an immune reaction developed immune tolerance to alpha-L-iduronidase. This finding has positive implications for long-term enzyme-replacement therapy in patients who have mucopolysaccharidosis I.  N. Ref:: 32

 

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[22]

TÍTULO / TITLE:  - Interleukin-2 receptor monoclonal antibodies in renal transplantation: meta-analysis of randomised trials.

REVISTA / JOURNAL:  - British Medical J (BMJ). Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://bmj.com/search.dtl 

      ●● Cita: British Medical J. (BMJ): <> 2003 Apr 12;326(7393):789.

      ●● Enlace al texto completo (gratuito o de pago) 1136/bmj.326.7393.789

AUTORES / AUTHORS:  - Adu D; Cockwell P; Ives NJ; Shaw J; Wheatley K

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Queen Elizabeth Hospital, Birmingham, B15 2TH. dwomoa.adu@uhb.nhs.uk

RESUMEN / SUMMARY:  - OBJECTIVE: To study the effect of interleukin-2 receptor monoclonal antibodies on acute rejection episodes, graft loss, deaths, and rate of infection and malignancy in patients with renal transplants. DESIGN: Meta-analysis of published data. DATA SOURCES: Medline, Embase, and Cochrane library for years 1996-2003 plus search of medical editors’ trial amnesty and contact with manufacturers of the antibodies. SELECTION OF STUDIES: Randomised controlled trials comparing interleukin-2 receptor antibodies with placebo or no additional treatment in patients with renal transplants receiving ciclosporin based immunosuppression. RESULTS: Eight randomised controlled trials involving 1871 patients met the selection criteria (although only 1858 patients were analysed). Interleukin-2 receptor antibodies significantly reduced the risk of acute rejection (odds ratio 0.51, 95% confidence interval 0.42 to 0.63). There were no significant differences in the rate of graft loss (0.78, 0.58 to 1.04), mortality (0.75, 0.46 to 1.23), overall incidence of infections (0.97, 0.77 to 1.24), incidence of cytomegalovirus infections (0.81, 0.62 to 1.04), or risk of malignancies at one year (0.82, 0.39 to 1.70). The different antibodies had a similar sized effect on acute rejection (test for heterogeneity P=0.7): anti-Tac (0.37, 0.16 to 0.89), BT563 (0.37, 0.1 to 1.38), basiliximab (0.56, 0.44 to 0.72), and daclizumab (0.46, 0.32 to 0.67). The reduction in acute rejections was similar for all ciclosporin based immunosuppression regimens (test for heterogeneity P=1.0). CONCLUSIONS: Adding interleukin-2 receptor antibodies to ciclosporin based immunosuppression reduces episodes of acute rejection at six months by 49%. There is no evidence of an increased risk of infective complications. Longer follow up studies are needed to confirm whether interleukin-2 receptor antibodies improve long term graft and patient survival.

 

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[23]

TÍTULO / TITLE:  - Stress management: MHC class I and class I-like molecules as reporters of cellular stress.

REVISTA / JOURNAL:  - Immunity 2003 Oct;19(4):469-77.

AUTORES / AUTHORS:  - Gleimer M; Parham P

INSTITUCIÓN / INSTITUTION:  - Program in Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.

RESUMEN / SUMMARY:  - The evolutionarily ancient intracellular stress response protects cells from the effects of external and internal forces which perturb cellular metabolism. Members of the major histocompatibility complex (MHC) class I-like superfamily act as cell surface indicators of the intracellular stress response. Cellular immunity employs these indicators as a cue for elimination of damaged, infected, and malignant cells, promoting the health of the individual and the evolutionary success of the species.  N. Ref:: 77

 

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[24]

TÍTULO / TITLE:  - Interleukin 2 receptor antagonists for renal transplant recipients: a meta-analysis of randomized trials.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 27;77(2):166-76.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000109643.32659.C4

AUTORES / AUTHORS:  - Webster AC; Playford EG; Higgins G; Chapman JR; Craig JC

INSTITUCIÓN / INSTITUTION:  - Cochrane Renal Group, Centre for Kidney Research, Children’s Hospital at Westmead, Westmead, NSW, Australia.

RESUMEN / SUMMARY:  - BACKGROUND: Interleukin 2 receptor antagonists (IL-2Ra) are increasingly used to treat renal transplant recipients. This study aims to systematically identify and summarize the effects of using IL-2Ra as induction immunosuppression, as an addition to standard therapy, or as an alternative to other antibody therapy. METHODS: Databases, reference lists, and abstracts of conference proceedings were searched extensively to identify relevant randomized controlled trials in all languages. Data were synthesized using the random effects model. Results are expressed as relative risk (RR), with 95% confidence intervals (CI). RESULTS: A total of 117 reports from 38 trials involving 4,893 participants were included. When IL-2Ra were compared with placebo (17 trials; 2,786 patients), graft loss was not significantly different at 1 year (14 trials: RR 0.84; CI 0.64-1.10) or 3 years (4 trials: RR 1.08; CI 0.71-1.64). Acute rejection was significantly reduced at 6 months (12 trials: RR 0.66; CI 0.59-0.74) and at 1 year (10 trials: RR 0.67; CI 0.60-0.75). At 1 year, cytomegalovirus infection (7 trials: RR 0.82; CI 0.65-1.03) and malignancy (9 trials: RR 0.67; CI 0.33-1.36) were not significantly different. When IL-2Ra were compared with other antibody therapy, no significant differences in treatment effects were demonstrated, but IL-2Ra had significantly fewer side effects. CONCLUSIONS: Given a 40% risk of rejection, seven patients would need treatment with IL-2Ra in addition to standard therapy, to prevent one patient from undergoing rejection, with no definite improvement in graft or patient survival. There is no apparent difference between basiliximab and daclizumab.

 

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[25]

TÍTULO / TITLE:  - Genetic interaction of CTLA-4 with HLA-DR15 in multiple sclerosis patients.

REVISTA / JOURNAL:  - Ann Neurol 2003 Jul;54(1):119-22.

      ●● Enlace al texto completo (gratuito o de pago) 1002/ana.10617

AUTORES / AUTHORS:  - Alizadeh M; Babron MC; Birebent B; Matsuda F; Quelvennec E; Liblau R; Cournu-Rebeix I; Momigliano-Richiardi P; Sequeiros J; Yaouanq J; Genin E; Vasilescu A; Bougerie H; Trojano M; Martins Silva B; Maciel P; Clerget-Darpoux F; Clanet M; Edan G; Fontaine B; Semana G

INSTITUCIÓN / INSTITUTION:  - Laboratoire Universitaire d’Immunologie (UPRES EA 1257, IFR 97) and Etablissement Francais du Sang Bretagne, Faculte de Medecine, Rennes, France.

RESUMEN / SUMMARY:  - Multiple sclerosis is a chronic inflammatory disease of the central nervous system with a genetic component. Until now, the more consistent association with the disease is found with the major histocompatibility complex, especially HLA-DRB1*1501-DQB1*0602 haplotype. In this report, we demonstrate the interaction of Cytotoxic T Lymphocyte-associated antigen 4 (CTLA-4 [CD152]) gene with DRB1*15 haplotype in multiple sclerosis genetic susceptibility. Our data were obtained from two European independent family-based studies including 610 multiple sclerosis family trios. Ann Neurol 2003;54:119-122  N. Ref:: 20

 

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[26]

TÍTULO / TITLE:  - Novel therapeutic molecular targets for prostate cancer: the mTOR signaling pathway and epidermal growth factor receptor.

REVISTA / JOURNAL:  - J Urol 2004 Feb;171(2 Pt 2):S41-3; discussion S44.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.ju.0000108100.53239.b7

AUTORES / AUTHORS:  - Tolcher AW

INSTITUCIÓN / INSTITUTION:  - Director Clinical Research, Institute for Drug Development Cancer Therapy and Research Center, San Antonio, Texas, USA.

RESUMEN / SUMMARY:  - PURPOSE: The scientific rationale and existing evidence for the use of novel molecular targets in the chemoprevention of cancer are reviewed, with special attention to prostate cancer. MATERIALS AND METHODS: A search for relevant literature on basic science and clinical trials was conducted using PubMed/MEDLINE. RESULTS: The emergence of molecularly targeted therapies for advanced malignancies creates an important opportunity to examine these agents for the chemoprevention of prostate cancer. Two critical targets in the proliferation and malignant transformation of normal cells, the PI3/Akt signal transduction pathway and the epidermal growth factor receptor, are currently the focus of several novel investigational therapies that are in late stage phase II and phase III studies. CONCLUSIONS: Research to date supports consideration of these novel molecular targets as future agents in the chemoprevention of prostate cancer.  N. Ref:: 28

 

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[27]

TÍTULO / TITLE:  - Tolerogenic dendritic cells induced by vitamin D receptor ligands enhance regulatory T cells inhibiting allograft rejection and autoimmune diseases.

REVISTA / JOURNAL:  - J Cell Biochem 2003 Feb 1;88(2):227-33.

      ●● Enlace al texto completo (gratuito o de pago) 1002/jcb.10340

AUTORES / AUTHORS:  - Adorini L; Penna G; Giarratana N; Uskokovic M

INSTITUCIÓN / INSTITUTION:  - BioXell, SpA, 20132 Milano, Italy. luciano.adorini@bioxell.com

RESUMEN / SUMMARY:  - Dendritic cells (DCs) not only induce but also modulate T cell activation. 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] induces DCs with a tolerogenic phenotype, characterized by decreased expression of CD40, CD80, and CD86 costimulatory molecules, low IL-12 and enhanced IL-10 secretion. We have found that a short treatment with 1,25(OH)(2)D(3) induces tolerance to fully mismatched mouse islet allografts that is stable to challenge with donor-type spleen cells and allows acceptance of donor-type vascularized heart grafts. This effect is enhanced by co-administration of mycophenolate mofetil (MMF), a selective inhibitor of T and B cell proliferation that has also effects similar to 1,25(OH)(2)D(3) on DCs. Graft acceptance is associated with an increased percentage of CD4(+)CD25(+) regulatory cells in the spleen and in the draining lymph node that can protect 100% of syngeneic recipients from islet allograft rejection. CD4(+)CD25(+) cells, able to inhibit the T cell response to a pancreatic autoantigen and to significantly delay disease transfer by pathogenic CD4(+)CD25(-) cells, are also induced by treatment of adult nonobese diabetic (NOD) mice with 1,25-dihydroxy-16,23Z-diene-26,27-hexafluoro-19-nor vitamin D(3) (BXL-698). This treatment arrests progression of insulitis and Th1 cell infiltration, and inhibits diabetes development at non-hypercalcemic doses. The enhancement of CD4(+)CD25(+) regulatory T cells, able to mediate transplantation tolerance and to arrest type 1 diabetes development by a short oral treatment with VDR ligands, suggests possible clinical applications of this approach.  N. Ref:: 41

 

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[28]

TÍTULO / TITLE:  - Immune activation: death, danger and dendritic cells.

REVISTA / JOURNAL:  - Curr Biol 2004 Jan 6;14(1):R30-2.

AUTORES / AUTHORS:  - Pulendran B

INSTITUCIÓN / INSTITUTION:  - Emory Vaccine Center, 954 Gatewood Road, Atlanta, Georgia 30329, USA. bpulend@rmy.emory.edu

RESUMEN / SUMMARY:  - Dendritic cells are critical for host immunity, and sense microbes with pathogen recognition receptors. New evidence indicates that these cells also sense uric acid crystals in dead cells, suggesting that the immune system is conscious not only of pathogens, but also of death and danger.  N. Ref:: 20

 

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[29]

TÍTULO / TITLE:  - Pathways for self-tolerance and the treatment of autoimmune diseases.

REVISTA / JOURNAL:  - Lancet 2001 Jun 30;357(9274):2115-21.

AUTORES / AUTHORS:  - Goodnow CC

INSTITUCIÓN / INSTITUTION:  - Australian Cancer Research Foundation, Genetics Laboratory, Medical Genome Centre, John Curtin School of Medical Research, Australian National University, Canberra, Australia.

RESUMEN / SUMMARY:  - Antigen delivers both immunogenic and tolerogenic signals to lymphocytes. The outcome of antigen exposure represents a complex integration of the timing of antigen binding with signals from many other immunogenic and tolerogenic costimulatory pathways. A road map of these signalling pathways is only beginning to be charted, revealing the mechansim of action and limitations of current immunotherapeutic agents and the points of attack for new agents. Ciclosporin and tacrolimus interfere with tolerogenic signals from antigen in addition to blocking immunogenic signals, thus preventing active establishment of tolerance. Corticosteroids inhibit a key immunogenic pathway, NFkappaB, and more specific inhibitors of this pathway may allow tolerance to be actively established while immune responses are blocked. New experimental therapies aim to mimic tolerogenic antigen signals by chronically stimulating antigen receptors with antigen or antibodies to the receptor, or aim to block costimulatory pathways involving CD40 ligand, B7, or interleukin 2. Obtaining the desired response with these strategies is unpredictable because many of these signals have both tolerogenic and immunogenic roles. The cause of autoimune diseases has been determined for several rare monogenic disorders, revealing inherited deficiencies in tolerogenic costimulatory pathways such as FAS. Common autoimmune disorders may have a biochemically related pathogenesis.  N. Ref:: 52

 

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[30]

- Castellano -

TÍTULO / TITLE:Guia de consenso sobre tuberculosis y tratamiento de la enfermedad inflamatoria intestinal con infliximab. Consensus guideline on tuberculosis and treatment of inflammatory bowel disease with infliximab. Spanish Working Group on Crohn Disease and Ulcerative Colitis.

REVISTA / JOURNAL:  - Gastroenterol Hepatol. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://db.doyma.es/ 

      ●● Cita: Gastroenterología & Hepatología: <> 2003 Jan;26(1):29-33.

AUTORES / AUTHORS:  - Obrador A; Lopez San Roman A; Munoz P; Fortun J; Gassull MA

INSTITUCIÓN / INSTITUTION:  - Servicio de Digestivo. Hospital Son Dureta. Palma de Mallorca. España.  N. Ref:: 19

 

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[31]

TÍTULO / TITLE:  - Chemokines, chemokine receptors, and allograft rejection.

REVISTA / JOURNAL:  - Immunity 2001 Apr;14(4):377-86.

AUTORES / AUTHORS:  - Nelson PJ; Krensky AM

INSTITUCIÓN / INSTITUTION:  - Medizinishe Poliklinik, Klinikum Innenstadt, Ludwig-Maximilians-University, Schillerstrasse 42, 80336, Munich, Germany. nelson@medpoli.med.uni-muenchen.de  N. Ref:: 40

 

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[32]

TÍTULO / TITLE:  - The target of rapamycin (TOR) proteins.

REVISTA / JOURNAL:  - Proc Natl Acad Sci U S A. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.pnas.org/ 

      ●● Cita: Proc Natl Acad Sci USA (PNAS): <> 2001 Jun 19;98(13):7037-44.

      ●● Enlace al texto completo (gratuito o de pago) 1073/pnas.121145898

AUTORES / AUTHORS:  - Raught B; Gingras AC; Sonenberg N

INSTITUCIÓN / INSTITUTION:  - Department of Biochemistry and McGill Cancer Centre, McGill University, 3655 Promenade Sir-William-Osler, Montreal, QC H3G 1Y6 Canada.

RESUMEN / SUMMARY:  - Rapamycin potently inhibits downstream signaling from the target of rapamycin (TOR) proteins. These evolutionarily conserved protein kinases coordinate the balance between protein synthesis and protein degradation in response to nutrient quality and quantity. The TOR proteins regulate (i) the initiation and elongation phases of translation, (ii) ribosome biosynthesis, (iii) amino acid import, (iv) the transcription of numerous enzymes involved in multiple metabolic pathways, and (v) autophagy. Intriguingly, recent studies have also suggested that TOR signaling plays a critical role in brain development, learning, and memory formation.  N. Ref:: 132

 

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[33]

TÍTULO / TITLE:  - Coeliac disease: dissecting a complex inflammatory disorder.

REVISTA / JOURNAL:  - Nat Rev Immunol 2002 Sep;2(9):647-55.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri885

AUTORES / AUTHORS:  - Sollid LM

INSTITUCIÓN / INSTITUTION:  - Institute of Immunology, Rikshospitalet, University of Oslo, 0027 Oslo, Norway. l.m.sollid@labmed.uio.no

RESUMEN / SUMMARY:  - The disease mechanisms of complex inflammatory disorders are difficult to define because of extensive interactions between genetic and environmental factors. Coeliac disease is a typical complex inflammatory disorder, but this disease is unusual in that crucial genetic and environmental factors have been identified. This knowledge has allowed functional studies of the predisposing HLA molecules, the identification of antigenic epitopes and detailed studies of disease-relevant T cells in coeliac disease. This dissection of the pathogenic mechanisms of coeliac disease has uncovered principles that are relevant to other chronic inflammatory diseases.  N. Ref:: 101

 

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[34]

TÍTULO / TITLE:  - Alpha E: no more rejection?

REVISTA / JOURNAL:  - J Exp Med. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jem.org/ 

      ●● Cita: J. Exp Med: <> 2002 Oct 7;196(7):873-5.

AUTORES / AUTHORS:  - Kilshaw PJ; Higgins JM

INSTITUCIÓN / INSTITUTION:  - The Babraham Institute, Cambridge, CB2 4AT, United Kingdom. peter.kilshaw@bbsrc.ac.uk  N. Ref:: 25

 

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[35]

TÍTULO / TITLE:  - HLA DNA typing and transplantation.

REVISTA / JOURNAL:  - Immunity 2001 Apr;14(4):347-56.

AUTORES / AUTHORS:  - Erlich HA; Opelz G; Hansen J

INSTITUCIÓN / INSTITUTION:  - Roche Molecular Systems, Alameda, CA 94501, USA. henry.erlich@roche.com  N. Ref:: 26

 

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[36]

TÍTULO / TITLE:  - Regulation of translation initiation by FRAP/mTOR.

REVISTA / JOURNAL:  - Genes Dev. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.genesdev.org/ 

      ●● Cita: Genes & Development: <> 2001 Apr 1;15(7):807-26.

      ●● Enlace al texto completo (gratuito o de pago) 1101/gad.887201

AUTORES / AUTHORS:  - Gingras AC; Raught B; Sonenberg N

INSTITUCIÓN / INSTITUTION:  - Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada.  N. Ref:: 236

 

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[37]

TÍTULO / TITLE:  - Innate immune responses to transplants: a significant variable with cadaver donors.

REVISTA / JOURNAL:  - Immunity 2001 Apr;14(4):369-76.

AUTORES / AUTHORS:  - Baldwin WM 3rd; Larsen CP; Fairchild RL

INSTITUCIÓN / INSTITUTION:  - Department of Pathology, Johns Hopkins Medical Institutes, Baltimore, MD 21205, USA. wbaldwin@jhmi.edu  N. Ref:: 70

 

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[38]

TÍTULO / TITLE:  - Rapamycin plays a new role as differentiator of vascular smooth muscle phenotype. focus on “The mTOR/p70 S6K1 pathway regulates vascular smooth muscle differentiation”.

REVISTA / JOURNAL:  - Am J Physiol Cell Physiol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://ajpcell.physiology.org/contents-by-date.0.shtml 

      ●● Cita: Am J Physiol Cell Physiol: <> 2004 Mar;286(3):C480-1.

      ●● Enlace al texto completo (gratuito o de pago) 1152/ajpcell.00526.2003

AUTORES / AUTHORS:  - Lucchesi PA  N. Ref:: 12

 

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[39]

TÍTULO / TITLE:  - CD3-specific antibody-induced active tolerance: from bench to bedside.

REVISTA / JOURNAL:  - Nat Rev Immunol 2003 Feb;3(2):123-32.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri1000

AUTORES / AUTHORS:  - Chatenoud L

INSTITUCIÓN / INSTITUTION:  - Centre de l’Association Claude Bernard sur les Maladies Autoimmunes and Hopital Necker Enfants Malades IRNEM, 161 Rue de Sevres, 75015 Paris, France. chatenoud@necker.fr

RESUMEN / SUMMARY:  - Although they were used initially as non-specific immunosuppressants in transplantation, CD3-specific monoclonal antibodies have elicited renewed interest owing to their capacity to induce immune tolerance. In mouse models of autoimmune diabetes, CD3-specific antibodies induce stable disease remission by restoring tolerance to pancreatic beta-cells. This phenomenon was extended recently to the clinic—preservation of beta-cell function in recently diagnosed patients with diabetes was achieved by short-term administration of a CD3-specific antibody. CD3-specific antibodies arrest ongoing disease by rapidly clearing pathogenic T cells from the target. Subsequently, they promote long-term T-cell-mediated active tolerance. Recent data indicate that transforming growth factor-beta-dependent CD4+CD25+ regulatory T cells might have a central role in this effect.  N. Ref:: 117

 

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[40]

TÍTULO / TITLE:  - Antigen presentation to naive CD4 T cells in the lymph node.

REVISTA / JOURNAL:  - Nat Immunol 2003 Aug;4(8):733-9.

      ●● Enlace al texto completo (gratuito o de pago) 1038/ni957

AUTORES / AUTHORS:  - Itano AA; Jenkins MK

INSTITUCIÓN / INSTITUTION:  - Department of Microbiology and the Center for Immunology, University of Minnesota, MMC 334, 420 Delaware St. SE, Minneapolis, Minnesota 55455, USA.

RESUMEN / SUMMARY:  - Although the presentation of peptide-major histocompatibility complex class II (pMHC class II) complexes to CD4 T cells has been studied extensively in vitro, knowledge of this process in vivo is limited. Unlike the in vitro situation, antigen presentation in vivo takes place within a complex microenvironment in which the movements of antigens, antigen-presenting cells (APCs) and T cells are governed by anatomic constraints. Here we review developments in the areas of lymph node architecture, APC subsets and T cell activation that have shed light on how antigen presentation occurs in the lymph nodes.  N. Ref:: 88

 

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[41]

TÍTULO / TITLE:  - Effects of MHC class I on HIV/SIV disease in primates.

REVISTA / JOURNAL:  - AIDS 2002;16 Suppl 4:S105-14.

AUTORES / AUTHORS:  - Carrington M; Bontrop RE

INSTITUCIÓN / INSTITUTION:  - Basic Research Program, SAIC Frederick, National Cancer Institute, Frederick, MD 21702, USA. carringt@ncifcrf.gov

RESUMEN / SUMMARY:  - Data indicate that resistance to HIV-1 disease involves an array of contrasting HLA genotypic effects that are subtle, but significant, particularly when these genetic effects are considered as a whole. Numerous reports attributing a role for HLA genotype in AIDS outcomes have been reported, and a few of these have been affirmed in multiple studies. Functional studies of immune cell recognition have provided clues to the underlying mechanisms behind some of the strongest HLA associations, suggesting the means by which relative resistance or susceptibility to the virus may occur. SIV infection in non-human primates has served as an invaluable model for understanding AIDS pathogenesis (in rhesus monkeys) and viral resistance (in chimpanzee). The effect of rhesus MHC class I molecules on the evolution of SIV has been convincingly described [19], and a recent study in humans has suggested that selection pressure conferred by HLA molecules is responsible for specific genetic variation in HIV-1 [114]. HIV-1 may eventually have conspicuous evolutionary effects on HLA and other AIDS restriction genes, a prolonged process that could have occurred in chimpanzee [92].To prevent such an outcome, it will be necessary to approach the disease from many perspectives, andapply comprehensively the knowledge gained to the successful control of the virus.  N. Ref:: 114

 

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[42]

TÍTULO / TITLE:  - IL-6: a magic potion for liver transplantation?

REVISTA / JOURNAL:  - Gastroenterology 2003 Jul;125(1):256-9.

AUTORES / AUTHORS:  - Selzner M; Graf R; Clavien PA  N. Ref:: 42

 

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[43]

TÍTULO / TITLE:  - Neuroimmunophilins: novel neuroprotective and neuroregenerative targets.

REVISTA / JOURNAL:  - Ann Neurol 2001 Jul;50(1):6-16.

AUTORES / AUTHORS:  - Guo X; Dillman JF 3rd; Dawson VL; Dawson TM

INSTITUCIÓN / INSTITUTION:  - Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

RESUMEN / SUMMARY:  - Cyclosporin A (CsA) and FK506 (tacrolimus) are immunosuppresants that are widely used in organ transplantation. CsA is an 11-member cyclic peptide, whereas FK506 is a macrolide antibiotic. Recently, these powerful and useful compounds have become of great interest to neuroscientists for their unique neuroprotective and neuroregenerative effects. These drugs and nonimmunosuppressive analogs protect neurons from the effects of glutamate excitotoxicity, focal ischemia, and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic cell death. They also stimulate functional recovery of neurons in a variety of neurologic injury paradigms. These drugs exert their effects via immunophilins, the protein receptors for these agents. The immunophilin ligands show particular promise as a novel class of neuroprotective and neuroregenerative agents that have the potential to treat a variety of neurologic disorders.  N. Ref:: 102

 

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[44]

TÍTULO / TITLE:  - The TCR triggering puzzle.

REVISTA / JOURNAL:  - Immunity 2001 Jun;14(6):665-8.

AUTORES / AUTHORS:  - van der Merwe PA

INSTITUCIÓN / INSTITUTION:  - Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. anton.vandermerwe@path.ox.ac.uk  N. Ref:: 28

 

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[45]

TÍTULO / TITLE:  - Gene therapy progress and prospects: gene therapy in organ transplantation.

REVISTA / JOURNAL:  - Gene Ther 2003 Apr;10(8):605-11.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.gt.3302020

AUTORES / AUTHORS:  - Bagley J; Iacomini J

INSTITUCIÓN / INSTITUTION:  - Transplantation Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA.

RESUMEN / SUMMARY:  - One major complication facing organ transplant recipients is the requirement for life-long systemic immunosuppression to prevent rejection, which is associated with an increased incidence of malignancy and susceptibility to opportunistic infections. Gene therapy has the potential to eliminate problems associated with immunosuppression by allowing the production of immunomodulatory proteins in the donor grafts resulting in local rather than systemic immunosuppression. Alternatively, gene therapy approaches could eliminate the requirement for general immunosuppression by allowing the induction of donor-specific tolerance. Gene therapy interventions may also be able to prevent graft damage owing to nonimmune-mediated graft loss or injury and prevent chronic rejection. This review will focus on recent progress in preventing transplant rejection by gene therapy.  N. Ref:: 47

 

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[46]

TÍTULO / TITLE:  - Making sense of mass destruction: quantitating MHC class I antigen presentation.

REVISTA / JOURNAL:  - Nat Rev Immunol 2003 Dec;3(12):952-61.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri1250

AUTORES / AUTHORS:  - Yewdell JW; Reits E; Neefjes J

INSTITUCIÓN / INSTITUTION:  - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0440, USA.  N. Ref:: 92

 

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[47]

TÍTULO / TITLE:  - The HFE Cys282Tyr mutation as a necessary but not sufficient cause of clinical hereditary hemochromatosis.

REVISTA / JOURNAL:  - Blood. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.bloodjournal.org/ 

      ●● Cita: Blood: <> 2003 May 1;101(9):3347-50.

      ●● Enlace al texto completo (gratuito o de pago) 1182/blood-2002-06-1747

AUTORES / AUTHORS:  - Beutler E

INSTITUCIÓN / INSTITUTION:  - Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA. beutler@scripps.edu  N. Ref:: 47

 

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[48]

TÍTULO / TITLE:  - Molecular aspects of iron absorption and HFE expression.

REVISTA / JOURNAL:  - Gastroenterology 2001 Dec;121(6):1489-96.

AUTORES / AUTHORS:  - Parkkila S; Niemela O; Britton RS; Fleming RE; Waheed A; Bacon BR; Sly WS

INSTITUCIÓN / INSTITUTION:  - Department of Anatomy and Cell Biology, University of Oulu, Oulu, Finland.

RESUMEN / SUMMARY:  - Hereditary hemochromatosis, a disease of iron overload, occurs in about 1 in 200-400 Caucasians. The gene mutated in this disorder is termed HFE. The product of this gene, HFE protein, is homologous to major histocompatibility complex class I proteins, but HFE does not present peptides to T cells. Based on recent structural, biochemical, and cell biological studies, transferrin receptor (TfR) is a ligand for HFE. This association directly links HFE protein to the TfR-mediated regulation of iron homeostasis. Although evidence is accumulating that binding of HFE to TfR is critical for the effects of HFE, the final pieces in the HFE puzzle have not been established. This review focuses on recent advances in HFE research and presents a hypothetical model of HFE function.  N. Ref:: 69

 

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[49]

TÍTULO / TITLE:  - Mhc-guided processing: binding of large antigen fragments.

REVISTA / JOURNAL:  - Nat Rev Immunol 2003 Aug;3(8):621-9.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri1149

AUTORES / AUTHORS:  - Sercarz EE; Maverakis E

INSTITUCIÓN / INSTITUTION:  - Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA. esercarz@tpims.org

RESUMEN / SUMMARY:  - Ever since the emergence of models for the processing and presentation of antigenic determinants by MHC class II molecules, the main view has been that proteins are unfolded, enzymatically cleaved into peptide lengths of about 12-25 amino acids and then loaded onto MHC class II molecules. There is, however, an alternative model stating that partially intact unfolding antigens are first bound by MHC class II molecules and then trimmed to fragments of a smaller size while remaining bound to the MHC class II molecule. In this analysis, we make the case that a considerable portion of the elutable peptide cargo belongs to this latter class.  N. Ref:: 61

 

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[50]

TÍTULO / TITLE:  - Specialization in tolerance: innate CD(4+)CD(25+) versus acquired TR1 and TH3 regulatory T cells.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 15;77(1 Suppl):S12-5.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000106471.23410.32

AUTORES / AUTHORS:  - Cottrez F; Groux H

INSTITUCIÓN / INSTITUTION:  - Institut national de la sante et de la recherche medicale, Hopital de l’Archet, Nice, France.

RESUMEN / SUMMARY:  - The regulation of immune responses to self-antigens is a complex process that involves maintaining self-tolerance while retaining the capacity to mount robust immune responses against invading microorganisms. Over the past few years, many new insights into this process have been gained, leading to the reemergence of the idea that regulatory T cells (Treg) are key players in immune regulation. These insights have raised fundamental questions concerning the definition of a Treg and what exactly constitutes T-cell-mediated suppression, identification of the signals and the cellular environment that promote the development and differentiation of these cells, and which signals maintain the homeostasis of the immune system. Thus far, the different models where Treg have been characterized cannot fully account for CD(4+)CD(25+) T cells. In this article, the authors propose the coexistence of two specialized types of CD(4+) Treg-innate and acquired-that differ in terms of their development, specificity, mechanisms, and sites of action.  N. Ref:: 33

 

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[51]

TÍTULO / TITLE:  - Interventions for mucous membrane pemphigoid and epidermolysis bullosa acquisita.

REVISTA / JOURNAL:  - Cochrane Database Syst Rev 2003;(1):CD004056.

AUTORES / AUTHORS:  - Kirtschig G; Murrell D; Wojnarowska F; Khumalo N

INSTITUCIÓN / INSTITUTION:  - Dermatology, Vrije Universiteit Medisch Centrum, PO Box 7057, Amsterdam, Netherlands, 1007 MB. G.Kirtschig@vumc.nl

RESUMEN / SUMMARY:  - BACKGROUND: Mucous membrane pemphigoid and epidermolysis bullosa acquisita are acquired autoimmune blistering diseases of the skin. Although they are rare, both can result in scarring of mucous membranes, which may lead to blindness and life threatening respiratory complications. OBJECTIVES: To assess the effects of treatments for mucous membrane pemphigoid and epidermolysis bullosa acquisita. SEARCH STRATEGY: Randomised Controlled Trials (RCTs) of patients with MMP or EBA were identified from MEDLINE and EMBASE from their inception to March 2000. The Cochrane Skin Group Specialised Register and the Cochrane Controlled Trials Register (CCTR) were last examined in February 2002. The bibliographies from identified studies were searched. The author who has conducted clinical trials in the field was contacted to identify unpublished trials. SELECTION CRITERIA: RCTs involving participants of any ages, and with a diagnosis confirmed by immunofluorescence. Where no RCTs were located, studies with other designs were considered. DATA COLLECTION AND ANALYSIS: Data were extracted from all included studies using a defined electronic data extraction protocol. Two reviewers evaluated the studies in terms of the inclusion criteria. The data from identified RCTs was extracted independently by three reviewers and subsequently checked for discrepancies. Any disagreements were resolved by discussion with each other and the fourth reviewer. Meta-analysis was not appropriate due to a lack of data. MAIN RESULTS: We found two small RCTs of MMP, both conducted in patients with severe eye involvement. The same author conducted both trials. In the first trial cyclophosphamide was superior to prednisone after six months of treatment; all 12 patients responded well to cyclophosphamide versus a good response in only five of 12 patients treated with prednisone (relative risk 2.40, 95% confidence interval 1.23 to 4.69). In the second trial all 20 patients treated with cyclophosphamide responded well to it after three months of treatment, but only 14 of 20 patients responded to the treatment with dapsone (relative risk 1.4, 95% confidence interval 1.07 to 1.90). We were not able to identify a RCT of therapeutic interventions in EBA. Thirty reports of uncontrolled studies of treatment for MMP involving five or more patients and 11 reports of treatment for EBA involving two or more patients were found, but were difficult to interpret. REVIEWER’S CONCLUSIONS: There is limited evidence (from two small trials) that severe ocular mucous membrane pemphigoid responds best to treatment with cyclophosphamide combined with corticosteroids, and that mild to moderate disease in most patients seems effectively suppressed by treatment with dapsone. It is difficult to make any treatment recommendations for EBA in the absence of reliable evidence sources.  N. Ref:: 59

 

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[52]

TÍTULO / TITLE:  - Genetically modified immunocompetent cells in HIV infection.

REVISTA / JOURNAL:  - Gene Ther 2001 Nov;8(21):1593-600.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.gt.3301569

AUTORES / AUTHORS:  - Palu G; Li Pira G; Gennari F; Fenoglio D; Parolin C; Manca F

INSTITUCIÓN / INSTITUTION:  - Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Italy.

RESUMEN / SUMMARY:  - Even in the era of highly active antiretroviral therapy (HAART), gene therapy (GT) can remain a promising approach for suppressing HIV infection, especially if complemented with other forms of pharmacological and immunological intervention. A large number of vectors and targets have been studied. Here we discuss the potential of genetically treated, antigen-specific immunocompetent cells for adoptive autologous immunotherapy of HIV infection. Cellular therapies with gene-modified CD8 and CD4 lymphocytes are aimed at reconstituting the antigen-specific repertoires that may be deranged as a consequence of HIV infection. Even if complete eradication of HIV from the reservoirs cannot be achieved, reconstitution of cellular immunity specific for opportunistic pathogens and for HIV itself is a desirable option to control progression of HIV infection and AIDS pathogenesis better.  N. Ref:: 103

 

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[53]

TÍTULO / TITLE:  - Gorillas with spondyloarthropathies express an MHC class I molecule with only limited sequence similarity to HLA-B27 that binds peptides with arginine at P2.

REVISTA / JOURNAL:  - J Immunol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jimmunol.org/ 

      ●● Cita: J. of Immunology: <> 2001 Mar 1;166(5):3334-44.

AUTORES / AUTHORS:  - Urvater JA; Hickman H; Dzuris JL; Prilliman K; Allen TM; Schwartz KJ; Lorentzen D; Shufflebotham C; Collins EJ; Neiffer DL; Raphael B; Hildebrand W; Sette A; Watkins DI

INSTITUCIÓN / INSTITUTION:  - Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.

RESUMEN / SUMMARY:  - The human MHC class I gene, HLA-B27, is a strong risk factor for susceptibility to a group of disorders termed spondyloarthropathies (SpAs). HLA-B27-transgenic rodents develop SpAs, implicating HLA-B27 in the etiology of these disorders. Several nonhuman primates, including gorillas, develop signs of SpAs indistinguishable from clinical signs of humans with SpAs. To determine whether SpAs in gorillas have a similar HLA-B27-related etiology, we analyzed the MHC class I molecules expressed in four affected gorillas. Gogo-B01, isolated from three of the animals, has only limited similarity to HLA-B27 at the end of the alpha1 domain. It differs by several residues in the B pocket, including differences at positions 45 and 67. However, the molecular model of Gogo-B*0101 is consistent with a requirement for positively charged residues at the second amino acid of peptides bound by the MHC class I molecule. Indeed, the peptide binding motif and sequence of individual ligands eluted from Gogo-B*0101 demonstrate that, like HLA-B27, this gorilla MHC class I molecule binds peptides with arginine at the second amino acid position of peptides bound by the MHC class I molecule. Furthermore, live cell binding assays show that Gogo-B*0101 can bind HLA-B27 ligands. Therefore, although most gorillas that develop SpAs express an MHC class I molecule with striking differences to HLA-B27, this molecule binds peptides similar to those bound by HLA-B27.  N. Ref:: 61

 

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[54]

TÍTULO / TITLE:  - Clinical consequences of iron overload in hemochromatosis homozygotes.

REVISTA / JOURNAL:  - Blood. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.bloodjournal.org/ 

      ●● Cita: Blood: <> 2003 May 1;101(9):3351-3; discussion 3354-8.

      ●● Enlace al texto completo (gratuito o de pago) 1182/blood-2002-11-3453

AUTORES / AUTHORS:  - Ajioka RS; Kushner JP

INSTITUCIÓN / INSTITUTION:  - Division of Hematology, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.  N. Ref:: 58

 

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[55]

TÍTULO / TITLE:  - Insulin/IGF and target of rapamycin signaling: a TOR de force in growth control.

REVISTA / JOURNAL:  - Trends Cell Biol 2003 Feb;13(2):79-85.

AUTORES / AUTHORS:  - Oldham S; Hafen E

INSTITUCIÓN / INSTITUTION:  - The Burnham Institute, La Jolla, CA 92037, USA.

RESUMEN / SUMMARY:  - ‘They come in all sizes.’ Apart from its origin and use in the clothing industry, this saying reflects the fact that the size of organisms spans an enormous range. Whether destined to be large or small, species grow in an organized fashion to reach their final specified size. For growth to proceed, food must be metabolized to liberate energy in the form of adenosine triphosphate (ATP) and protein building blocks in the form of amino acids. One major orchestrator of this complex growth process in diverse metazoan species is the insulin/insulin-like growth factor (IGF) system. This review summarizes current studies primarily from Drosophila regarding the function of the insulin/IGF system in the control of growth.  N. Ref:: 75

 

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[56]

TÍTULO / TITLE:  - Review article: medical treatment of moderate to severe Crohn’s disease.

REVISTA / JOURNAL:  - Aliment Pharmacol Ther 2003 Jun;17 Suppl 2:23-30.

AUTORES / AUTHORS:  - Scribano M; Prantera C

INSTITUCIÓN / INSTITUTION:  - Division of Gastroenterology, Azienda Ospedaliera S.Camillo-Forlanini, Rome, Italy.

RESUMEN / SUMMARY:  - The treatment for patients with Crohn’s disease of moderate to severe activity includes traditional drugs, such as corticosteroids, the primary therapy for these forms of disease, able to induce the remission of symptoms in a high percentage of patients. Because of the side-effects produced by systemic steroids, a new glucocorticoid derivative, budesonide, which acts locally in the mucosa, has recently been introduced with positive results. On the assumption that intestinal bacteria play a role in the causing Crohn’s disease symptoms, antibiotics are often used in the treatment of active phases, as an alternative to or in association with steroids. The most widely employed antibiotics are metronidazole and ciprofloxacin. Immunosuppressors, such as azathioprine and 6-mercaptopurine, are useful for the treatment of chronic active disease and for maintaining remission, but they have only a marginal role in the therapy of an acute flare-up of Crohn’s disease. Methotrexate acts more rapidly and its use in patients with active disease resistant to standard therapy is of interest. The discovery of biological agents represents a new era in the management of patients. To date, infliximab is the more extensively studied biological therapy in the treatment of Crohn’s disease and clinical studies have demonstrated its efficacy in inducing remission of refractory disease.  N. Ref:: 59

 

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[57]

TÍTULO / TITLE:  - Sarcoidosis.

REVISTA / JOURNAL:  - Lancet 2003 Mar 29;361(9363):1111-8.

AUTORES / AUTHORS:  - Baughman RP; Lower EE; du Bois RM

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine, University of Cincinnati, Cincinnati Medical Center, Cincinnati, OH 45267-0565, USA. bob.baughman@uc.edu

RESUMEN / SUMMARY:  - There have been several new insights into the cause and treatment of sarcoidosis. Studies of genetic variation have shown that specific genetic polymorphisms are associated with increased risk of disease or affect disease presentation. These polymorphisms include variation of MHC and cytokines such as tumour necrosis factor (TNF). Not all investigators have come to the same conclusion, suggesting an interaction of various factors, including the patient’s ethnic origin. Treatment of sarcoidosis varies considerably. Patients with symptomatic disease for more than 2-5 years have been of particular interest. Corticosteroids remain the standard of care in such cases, but immunosuppressive drugs have proved steroid-sparing in many patients. New agents, including pentoxifylline, thalidomide, and infliximab have proved useful in selected cases. The effectiveness of these agents seems to lie in their ability to block TNF, especially in the treatment of chronic disease.  N. Ref:: 117

 

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[58]

TÍTULO / TITLE:  - The tolerant recipient: looking great in someone else’s genes.

REVISTA / JOURNAL:  - J Clin Invest. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.jci.org/ 

      ●● Cita: J Clinical Investigation: <> 2001 Jan;107(1):33-4.

AUTORES / AUTHORS:  - Rosengard BR; Turka LA

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, University of Pennsylvania, Philadelphia, Pennsylvania, USA.  N. Ref:: 18

 

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[59]

TÍTULO / TITLE:  - Human CD(4+)CD(25+) regulatory T cells and infectious tolerance.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 15;77(1 Suppl):S23-5.

AUTORES / AUTHORS:  - Stassen M; Schmitt E; Jonuleit H

INSTITUCIÓN / INSTITUTION:  - Institute of Immunology, Johannes Gutenberg-University, Mainz, Germany. michael.stassen@gmx.de.

RESUMEN / SUMMARY:  - Control of autoaggressive T cells by regulatory T cells (Treg) is essential to ensuring peripheral tolerance. Several subsets of CD(4+) T cells with suppressive properties have been described, including induced T helper (Th) type 3 and T regulatory (Tr) type 1 cells and naturally occurring CD(4+)CD(25+) Treg. CD(4+)CD(25+) Treg suppress the response of conventional T cells in a cell contact-dependent manner, whereas Th3 and Tr1 cells produce immunosuppressive cytokines. Two subsets of human CD(4+)CD(25+) Treg, characterized by expression of the integrins alpha4beta7 or alpha4beta1, are able to convey suppressive capacity to conventional CD(4+) T cells, thereby generating Th suppressor cells (Th(sup)). One outstanding feature is the generation of Th(sup) with distinct properties. alpha4beta7 Treg induce Tr1-like interleukin (IL)-10-producing Th(sup), whereas alpha4beta1 Treg induce Th3-like Th(sup), which produce transforming growth factor (TGF)-beta. Thus, our findings reconcile contradictory results clearly demonstrating that suppression is contact dependent in vitro but mediated by soluble factors (IL-10 and TGF-beta) in vivo.  N. Ref:: 21

 

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[60]

TÍTULO / TITLE:  - Glucocorticoids and invasive fungal infections.

REVISTA / JOURNAL:  - Lancet 2003 Nov 29;362(9398):1828-38.

AUTORES / AUTHORS:  - Lionakis MS; Kontoyiannis DP

INSTITUCIÓN / INSTITUTION:  - Department of Infectious Diseases, Infection Control and Employee Health, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

RESUMEN / SUMMARY:  - Since the 1990s, opportunistic fungal infections have emerged as a substantial cause of morbidity and mortality in profoundly immunocompromised patients. Hypercortisolaemic patients, both those with endogenous Cushing’s syndrome and, much more frequently, those receiving exogenous glucocorticoid therapy, are especially at risk of such infections. This vulnerability is attributed to the complex dysregulation of immunity caused by glucocorticoids. We critically review the spectrum and presentation of invasive fungal infections that arise in the setting of hypercortisolism, and the ways in which glucocorticoids contribute to their pathogenesis. A better knowledge of the interplay between glucocorticoid-induced immunosuppression and invasive fungal infections should assist in earlier recognition and treatment of such infections. Efforts to decrease the intensity of glucocorticoid therapy should help to improve outcomes of opportunistic fungal infections.  N. Ref:: 135

 

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[61]

TÍTULO / TITLE:  - Cell survival and clinical outcome following intrastriatal transplantation in Parkinson disease.

REVISTA / JOURNAL:  - J Neuropathol Exp Neurol 2001 Aug;60(8):741-52.

AUTORES / AUTHORS:  - Hagell P; Brundin P

INSTITUCIÓN / INSTITUTION:  - Department of Clinical Neuroscience, University Hospital, Lund University, Sweden.

RESUMEN / SUMMARY:  - Intrastriatal transplantation of embryonic dopaminergic neurons is currently explored as a restorative cell therapy for Parkinson disease (PD). Clinical results have varied, probably due to differences in transplantation methodology and patient selection. In this review, we assess clinical trials and autopsy findings in grafted PD patients and suggest that a minimum number of surviving dopaminergic neurons is required for a favorable outcome. Restoration of [18F]-fluorodopa uptake in the putamen to about 50% of the normal mean seems necessary for moderate to marked clinical benefit to occur. Some studies indicate that this may require mesencephalic tissue from 3-5 human embryos implanted into each hemisphere. The volume, density and pattern of fiber outgrowth and reinnervation, as well as functional integration and dopamine release. are postulated as additional important factors for an optimal clinical outcome. For neural transplantation to become a feasible therapeutic alternative in PD, graft survival must be increased and the need for multiple donors of human embryonic tissue substantially decreased or alternate sources of donor tissue developed. Donor cells derived from alternative sources should demonstrate features comparable to those associated with successful implantation of human embryonic tissue before clinical trials are considered.  N. Ref:: 62

 

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[62]

TÍTULO / TITLE:  - Anatomical basis of tolerance and immunity to intestinal antigens.

REVISTA / JOURNAL:  - Nat Rev Immunol 2003 Apr;3(4):331-41.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri1057

AUTORES / AUTHORS:  - Mowat AM

INSTITUCIÓN / INSTITUTION:  - Department of Immunology and Bacteriology, Western Infirmary, Glasgow G11 6NT, UK. a.m.mowat@clinmed.gla.ac.uk

RESUMEN / SUMMARY:  - The intestinal immune system has to discriminate between harmful and beneficial antigens. Although strong protective immunity is essential to prevent invasion by pathogens, equivalent responses against dietary proteins or commensal bacteria can lead to chronic disease. These responses are normally prevented by a complex interplay of regulatory mechanisms. This article reviews the unique aspects of the local microenvironment of the intestinal immune system and discuss how these promote the development of regulatory responses that ensure the maintenance of homeostasis in the gut.  N. Ref:: 99

 

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[63]

TÍTULO / TITLE:  - Scaffolding of antigen receptors for immunogenic versus tolerogenic signaling.

REVISTA / JOURNAL:  - Nat Immunol 2003 Nov;4(11):1057-64.

      ●● Enlace al texto completo (gratuito o de pago) 1038/ni1001

AUTORES / AUTHORS:  - Jun JE; Goodnow CC

INSTITUCIÓN / INSTITUTION:  - Australian Cancer Research Foundation Genetics Laboratory and Medical Genome Centre, John Curtin School of Medical Research, Australian National University, Canberra ACT 2601, Australia.

RESUMEN / SUMMARY:  - Lymphocyte antigen receptors are responsible for inducing the opposite responses of immunity or tolerance. How the correct polarity of antigen receptor signaling is encoded has been an enduring enigma. Here we summarize recent advances defining key scaffolding molecules, CARMA1 (also known as CARD11) and the Cbl family of ubiquitin ligases, required for either immunogenic or tolerogenic signaling by antigen receptors. These scaffolding proteins may determine the polarity of response to antigen by promoting assembly around antigen receptors of competing multiprotein signal complexes: immunosomes versus tolerosomes. Each of the factors that influence immunogenicity or tolerogenicity—stage of lymphocyte differentiation, concurrent engagement of inhibitory or costimulatory receptors, extent of receptor crosslinking, and prior antigen experience—may be integrated in lymphocytes through their capacity to influence the probability of assembling immunosomes versus tolerosomes.  N. Ref:: 111

 

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[64]

TÍTULO / TITLE:  - Effect of dexamethasone on beta2-adrenergic desensitization in airway smooth muscle: role of the ARG19 polymorphism.

REVISTA / JOURNAL:  - Chest. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.chestjournal.org/ 

      ●● Cita: Chest: <> 2003 Mar;123(3 Suppl):368S-9S.

AUTORES / AUTHORS:  - Moore PE; Calder MM; Silverman ES; Panettieri RA Jr; Shore SA

INSTITUCIÓN / INSTITUTION:  - Departments of Pediatrics and Pharmacology (Dr. Moore and Mr. Calder), Vanderbilt University School of Medicine, Nashville, TN 37232-2586, USA.  N. Ref:: 1

 

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[65]

TÍTULO / TITLE:  - Antiadhesion molecule therapy in inflammatory bowel disease.

REVISTA / JOURNAL:  - Inflamm Bowel Dis 2002 Jul;8(4):291-300.

AUTORES / AUTHORS:  - van Assche G; Rutgeerts P

INSTITUCIÓN / INSTITUTION:  - Division of Gastroenterology, University Hospital Leuven, Belgium. gert.vanassche@uz.kuleuven.ac.be

RESUMEN / SUMMARY:  - Adhesion molecules regulate the influx of leukocytes in normal and inflamed gut. Some of these molecules such as MadCAM-1 are specific for the gastrointestinal endothelium, but in inflammatory bowel diseases most of the adhesion factors are up-regulated. Adhesion molecules also are involved in local lymphocyte stimulation and antigen presentation within the intestinal mucosa. Recently, therapeutic compounds directed against trafficking of lymphocytes toward the gut mucosa have been designed, and are being developed as a novel class of drugs in the treatment of Crohn’s disease (CD) and ulcerative colitis. This review deals with the immunological aspects of leukocyte trafficking focused on gut homing of T cells. Secondly, the changes in adhesion molecules and T-cell trafficking during intestinal inflammation are discussed. Finally, we review the clinical data that have been gathered in trials of biological therapies directed against adhesion molecules. Both antiintercellular adhesion molecule-1 (ICAM-1) and anti-alpha4 integrin strategies are being developed. Trials with the anti-ICAM-1 antisense oligonucleotide, ISIS-2302, in steroid-refractory CD have provided conflicting efficacy data. The anti-alpha4 integrin antibodies natalizumab (Antegren) and LDP-02 are in phase III and phase II trials, respectively. In the near future, these novel biological agents may prove valuable therapeutic tools in the management of refractory IBD.  N. Ref:: 56

 

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[66]

TÍTULO / TITLE:  - New agents in acute myeloid leukemia and other myeloid disorders.

REVISTA / JOURNAL:  - Cancer 2004 Feb 1;100(3):441-54.

      ●● Enlace al texto completo (gratuito o de pago) 1002/cncr.11935

AUTORES / AUTHORS:  - Ravandi F; Kantarjian H; Giles F; Cortes J

INSTITUCIÓN / INSTITUTION:  - Department of Leukemia, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA. fravandi@mdanderson.org

RESUMEN / SUMMARY:  - Over the past several decades, improvements in chemotherapeutic agents and supportive care have resulted in significant progress in treating patients with acute myeloid leukemia (AML). More recently, advances in understanding the biology of AML have resulted in the identification of new therapeutic targets. The success of all-trans-retinoic acid in acute promyelocytic leukemia and of imatinib mesylate in chronic myeloid leukemia have demonstrated that targeted therapy may be more effective and less toxic when well defined targets are available. At the same time, understanding mechanisms of drug resistance and means to overcome them has led to modification of some of the existing cytotoxic agents. Rational design and conduct of clinical trials is necessary to ensure that the full potential of these new agents is realized.  N. Ref:: 140

 

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[67]

TÍTULO / TITLE:  - Effects of glatiramer acetate on relapse rate and accumulated disability in multiple sclerosis: meta-analysis of three double-blind, randomized, placebo-controlled clinical trials.

REVISTA / JOURNAL:  - Mult Scler 2003 Aug;9(4):349-55.

AUTORES / AUTHORS:  - Boneschi FM; Rovaris M; Johnson KP; Miller A; Wolinsky JS; Ladkani D; Shifroni G; Comi G; Filippi M

INSTITUCIÓN / INSTITUTION:  - Department of Neuroscience, Scientific Institute, University H San Raffaele, Milan, Italy.

RESUMEN / SUMMARY:  - Three randomized, double-blind, placebo-controlled trials have shown that glatiramer acetate (GA) is effective in reducing relapse rate in patients with relapsing-remitting (RR) multiple sclerosis (MS). Using raw data pooled from 540 patients, we performed a meta-analysis of these three trials, to investigate whether the extent of GA efficacy varies according to disease-related variables at study entry. Three regression models were developed to assess the efficacy of GA on the annualized relapse rate (primary outcome measure), on the total number of on-trial relapses and on the time to first relapse. We also explored the efficacy of GA on accumulated disability and the potential role of baseline clinical variables as predictors of relapse-rate variables and treatment efficacy. The mean adjusted annualized relapse rate on study was 1.14 in the pooled placebo-treated subjects and 0.82 in the pooled GA group (P = 0.004), indicating an average reduction in annualized relapse rate of 28%. About a one third reduction of the total number of on-trial relapses was also observed in patients receiving GA (P < 0.0001), who had a median time to the first relapse of 322 days versus a median time to the first relapse of 219 days seen in those receiving placebo (P = 0.01). A beneficial effect on accumulated disability was also found (risk ratio of 0.6; 95%; CI = 0.4-0.9; P = 0.02). The drug assignment (P = 0.004), baseline EDSS score (P = 0.02) and number of relapses during the two years prior to study entry (P = 0.002) were significant predictors of on-trial annualized relapse rate. No other demographic or clinical variable at baseline significantly influenced the treatment effect. This meta-analysis reaffirms the effectiveness of GA in reducing relapse rate and disability accumulation in RRMS, at a magnitude comparable to that of other available immunomodulating treatments. It also suggests that GA efficacy is not significantly influenced by the patients’ clinical characteristics at the time of treatment initiation.

 

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[68]

TÍTULO / TITLE:  - Virus evasion of MHC class I molecule presentation.

REVISTA / JOURNAL:  - J Immunol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jimmunol.org/ 

      ●● Cita: J. of Immunology: <> 2003 Nov 1;171(9):4473-8.

AUTORES / AUTHORS:  - Petersen JL; Morris CR; Solheim JC

INSTITUCIÓN / INSTITUTION:  - Eppley Institute for Research in Cancer and Allied Diseases, Omaha, NE 68198-6805, USA.  N. Ref:: 97

 

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[69]

TÍTULO / TITLE:  - HLA complex genes in type 1 diabetes and other autoimmune diseases. Which genes are involved?

REVISTA / JOURNAL:  - Trends Genet 2001 Feb;17(2):93-100.

AUTORES / AUTHORS:  - Undlien DE; Lie BA; Thorsby E

INSTITUCIÓN / INSTITUTION:  - Institute of Immunology, The National Hospital and University of Oslo, N-0027, Oslo, Norway. d.e.undlien@rh.uio.no

RESUMEN / SUMMARY:  - The predisposition to develop a majority of autoimmune diseases is associated with specific genes within the human leukocyte antigen (HLA) complex. However, it is frequently difficult to determine which of the many genes of the HLA complex are directly involved in the disease process. The main reasons for these difficulties are the complexity of associations where several HLA complex genes might be involved, and the strong linkage disequilibrium that exists between the genes in this complex. The latter phenomenon leads to secondary disease associations, or what has been called ‘hitchhiking polymorphisms’. Here, we give an overview of the complexity of HLA associations in autoimmune disease, focusing on type 1 diabetes and trying to answer the question: how many and which HLA genes are directly involved?  N. Ref:: 40

 

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[70]

TÍTULO / TITLE:  - Quality control of MHC class I maturation.

REVISTA / JOURNAL:  - Faseb J. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.fasebj.org/ 

      ●● Cita: FASEB Journal: <> 2004 Jan;18(1):31-8.

      ●● Enlace al texto completo (gratuito o de pago) 1096/fj.03-0846rev

AUTORES / AUTHORS:  - Paulsson KM; Wang P

INSTITUCIÓN / INSTITUTION:  - Rayne Institute, Centre for Molecular Medicine, Department of Medicine, University College of London, 5 University St., London WC1E 6JJ, UK. k.paulsson@ucl.ac.uk

RESUMEN / SUMMARY:  - Assembly of MHC class I molecules in the ER is regulated by the so-called loading complex (LC). This multiprotein complex is of definite importance for class I maturation, but its exact organization and order of assembly are not known. Evidence implies that the quality of peptides loaded onto class I molecules is controlled at multiple stages during MHC class I assembly. We recently found that tapasin, an important component of the LC, interacts with COPI-coated vesicles. Biochemical studies suggested that the tapa-sin-COPI interaction regulates the retrograde transport of immature MHC class I molecules from the Golgi network back to the ER. Also other findings now propose that in addition to the peptide-loading control, the quality control of MHC class I antigen presentation includes the restriction of export of suboptimally loaded MHC class I molecules to the cell surface. In this review, we use recent studies of tapasin to examine the efficiency of TAP, the LC constitution, ER quality control of class I assembly, and peptide optimization. The concepts of MHC class I recycling and ER retention are also discussed.  N. Ref:: 73

 

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[71]

TÍTULO / TITLE:  - The transplantation of hematopoietic stem cells after non-myeloablative conditioning: a cellular therapeutic approach to hematologic and genetic diseases.

REVISTA / JOURNAL:  - Immunol Res 2003;28(1):13-24.

AUTORES / AUTHORS:  - Maris M; Storb R

INSTITUCIÓN / INSTITUTION:  - Fred Hutchinson Cancer Research Center, and University of Washington, Seattle, WA, USA. mmaris@fhcrc.org

RESUMEN / SUMMARY:  - Originally, allogeneic hematopoietic stem cell transplantation (HSCT) was viewed as a form of rescue from the marrow lethal effects of high doses of chemo-radiotherapy used to both eradicate malignancy and to provide sufficient immunosuppression to ensure allogeneic engraftment. Clear evidence of a therapeutic graft-versus-tumor (GVT) effect mediated by allogeneic effector cells (T cells) has prompted the exploration of HSCT regimens that rely solely upon host immunosuppression (non-myeloablative) to facilitate allogeneic donor engraftment. The engrafted donor effector cells are then used to accomplish the task of eradicating host malignant cells. The non-myeloblative regimen developed in Seattle uses 2 Gy total body irradiation (TBI) before transplant followed by postgrafting cyclosporine (CSP) and mycophenolate mofetil (MMF). This regimen resulted in initial mixed donor-host chimerism in all patients with hematologic malignancies and genetic disorders who received HLA-matched sibling allografts. The 17% incidence of graft rejection was reduced to 3% with the addition of fludarabine, 30 mg/m2/day on d -4, -3, and -2. The non-myeloablative combination of fludarabine/TBI has also been successful at achieving high engraftment rates in recipients of 10 of 10 HLA antigen matched unrelated donor HSCTs in patients with hematologic malignancies. By reducing acute toxicities relative to conventional HSCT, most patients have received their pre- and post-HSCT therapy almost exclusively as outpatients. Acute and chronic GVHD occur after non-myeloablative HSCT, but the incidence and severity appear less compared to conventional HSCT. As in conventional transplants, immune dysregulation from GVHD and its treatment and delayed reconstitution of immune function continue to present risks to patients who have otherwise undergone successful non-myeloablative HSCT. Cellular therapeutic effects have been observed after non-myeloablative HSCT such as correction of inherited genetic disorders, and eradication of hematologic malignant diseases and renal cell carcinoma via GVT responses.  N. Ref:: 52

 

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[72]

TÍTULO / TITLE:  - Sublingual immunotherapy for allergic rhinitis.

REVISTA / JOURNAL:  - Cochrane Database Syst Rev 2003;(2):CD002893.

AUTORES / AUTHORS:  - Wilson DR; Torres LI; Durham SR

INSTITUCIÓN / INSTITUTION:  - Upper Respiratory Medicine, Imperial College School of Medicine at the National Heart & Lung Institute, Dovehouse Street, London, UK, SW3 6LR. duncw 99@yahoo.co.uk

RESUMEN / SUMMARY:  - BACKGROUND: Allergic rhinitis is a common condition which, at its most severe, can significantly impair quality of life despite optimal treatment with antihistamines and topical nasal corticosteroids. Allergen injection immunotherapy significantly reduces symptoms and medication requirements in allergic rhinitis but its use is limited by the possibility of severe systemic reactions. There has therefore been considerable interest in alternative routes for delivery of allergen immunotherapy, particularly the sublingual route. OBJECTIVES: To evaluate the efficacy of sublingual immunotherapy (SLIT), compared with placebo, for reductions in symptoms and medication requirements. SEARCH STRATEGY: The Cochrane Controlled Trials Register, MEDLINE (1966-2002), EMBASE (1974-2002) and Scisearch were searched, up to September 2002, using the terms (Rhin* OR hay fever) AND (immunotherap* OR desensiti*ation) AND (sublingual). SELECTION CRITERIA: All studies identified by the searches were assessed by the reviewers to identify randomised controlled trials involving participants with symptoms of allergic rhinitis and proven allergen sensitivity, treated with SLIT or corresponding placebo. DATA COLLECTION AND ANALYSIS: Data from identified studies were abstracted onto a standard extraction sheet and subsequently entered into RevMan 4.1. Analysis was performed by the method of Standardised Mean Differences (SMD) using a random effects model. P values <0.05 were considered statistically significant. Subgroup analyses were performed according to the type of allergen administered, the age of participants and the duration of treatment. MAIN RESULTS: Twenty two trials involving 979 patients were included. There were 6 trials of SLIT for House Dust Mite allergy, 5 for Grass Pollen, 5 for Parietaria, 2 for Olive and one each for, Ragweed, Cat, Tree and Cupressus. Four studies enrolled exclusively children. Seventeen studies administered the allergen by sublingual drops subsequently swallowed, 3 by drops subsequently spat out and 2 by sublingual tablets. Eight studies involved treatment for less than 6 months, 10 studies for 6-12 months and 4 studies for greater than 12 months. All included studies were double-blind placebo-controlled trials of parallel group design. Concealment of treatment allocation was considered adequate in all studies and the use of identical placebo preparations was almost universal. There was significant heterogeneity, most likely due to widely differing scoring systems between studies, for most comparisons. Overall there was a significant reduction in both symptoms (SMD -0.34, 95% confidence interval -0.69 to -0.15; p=0.002) and medication requirements (SMD -0.43 [-0.63, -0.23]; p=0.00003) following immunotherapy. Subgroup analyses failed to identify a disproportionate benefit of treatment according to the allergen administered. There was no significant reduction in symptoms and medication scores in those studies involving only children but total numbers of participants were small, casting doubt on the validity of the conclusion. Increasing duration of treatment does not clearly increase efficacy. The total dose of allergen administered may be important but insufficient data were available to analyse this factor. REVIEWER’S CONCLUSIONS: SLIT is a safe treatment which significantly reduces symptoms and medication requirements in allergic rhinitis. The size of this benefit compared to that of other available therapies, particularly injection immunotherapy, is not clear, having been assessed directly in very few studies. Further research is required concentrating on optimising allergen dosage and patient selection.  N. Ref:: 41

 

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[73]

TÍTULO / TITLE:  - DR, DQ, and you: MHC alleles and autoimmunity.

REVISTA / JOURNAL:  - J Clin Invest. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.jci.org/ 

      ●● Cita: J Clinical Investigation: <> 2001 Apr;107(7):795-6.

AUTORES / AUTHORS:  - Sonderstrup G; McDevitt HO

INSTITUCIÓN / INSTITUTION:  - Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305-5124, USA. gretes@stanford.edu  N. Ref:: 12

 

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[74]

TÍTULO / TITLE:  - Potential roles of protein oxidation and the immunoproteasome in MHC class I antigen presentation: the ‘PrOxI’ hypothesis.

REVISTA / JOURNAL:  - Arch Biochem Biophys 2004 Mar 1;423(1):88-96.

      ●● Enlace al texto completo (gratuito o de pago) 1016/j.abb.2003.12.001

AUTORES / AUTHORS:  - Teoh CY; Davies KJ

INSTITUCIÓN / INSTITUTION:  - Ethel Percy Andrus Gerontology Center and Division of Molecular and Computational Biology, The University of Southern California, Los Angeles, CA 90089-0191, USA.

RESUMEN / SUMMARY:  - The major histocompatibility complex (MHC) class I (MHC-I) antigen presentation system is responsible for the cell-surface presentation of self-proteins and intracellular viral proteins. This pathway is important in screening between self, and non-self or infected cells. In this pathway, proteins are partially degraded to peptides in the cytosol and targeted to the cell surface bound to an MHC-I receptor protein. At the cell surface, T cells bypass cells displaying self-peptides but destroy others displaying foreign antigens. Cells contain several isoforms of the proteasome, but it is thought that the immunoproteasome is the major form involved in generating peptides for the MHC-I pathway. How all intracellular proteins are targeted for MHC-I processing is unclear. Oxidative stress is experienced by all cells, and all proteins are exposed to oxidation. We propose that oxidative modification makes proteins susceptible to degradation by the immunoproteasome. This could be called the protein oxidation and immunoproteasome or ‘PrOxI’ hypothesis of MHC-I antigen processing. Protein oxidation may, thus, be a universal mechanism for peptide generation and presentation in the MHC-I pathway.  N. Ref:: 123

 

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[75]

TÍTULO / TITLE:  - Current directions in hemochromatosis research: towards an understanding of the role of iron overload and the HFE gene mutations in the development of clinical disease.

REVISTA / JOURNAL:  - Nutr Rev 2003 Jan;61(1):38-42.

AUTORES / AUTHORS:  - Neff LM

INSTITUCIÓN / INSTITUTION:  - Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, New England Medical Center, Boston, MA, USA.

RESUMEN / SUMMARY:  - Since the discovery of a candidate gene (HFE) thought to be involved in the development of hereditary hemochromatosis, there has been much interest in the potential use of genetic testing as a screening tool for the disease in the general population. However, a recent study suggests that less than 1% of subjects who are homozygous for the gene mutations will go on to develop the full-blown disease of hereditary hemochromatosis, historically termed “bronzed diabetes.” The study also suggests that homozygotes have no higher risk of mortality or of any clinically significant morbidity than normal control subjects. This conclusion contradicts earlier findings that linked iron overload and HFE mutations to a number of devastating diseases, including cardiovascular disease, diabetes, and cancer.  N. Ref:: 15

 

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[76]

TÍTULO / TITLE:  - Endothelial expression of MHC class II molecules in autoimmune disease.

REVISTA / JOURNAL:  - Curr Pharm Des 2004;10(2):129-43.

AUTORES / AUTHORS:  - Turesson C

INSTITUCIÓN / INSTITUTION:  - Department of Rheumatology, Malmo University Hospital, Malmo, Sweden. turesson.carl@mayo.edu

RESUMEN / SUMMARY:  - Major histocompatibility complex (MHC) class II molecules are up-regulated on endothelial cells in human allografts, and are thought to be involved in graft rejection. The MHC class II subtypes HLA-DR, DQ and DP regulate T cell dependent immune responses, and aberrant expression could be important in autoimmunity. Increased endothelial MHC class II expression has been demonstrated in several autoimmune diseases, including myocarditis with dilated cardiomyopathy, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Recent data suggest that there is an association between endothelial expression of MHC class II molecules and diffuse endothelial dysfunction, which may be part of the explanation of the increased risk of cardiovascular disease in patients with RA, SLE and other chronic inflammatory conditions. MHC class II transcription is in part genetically determined. Cytokine induced up-regulation of MHC class II molecules can be inhibited in vitro by antioxidants and different drugs, such as cyclosporin and statins. Research on the development of new treatments for systemic autoimmune diseases and cardiovascular disease should include evaluation of effects on endothelial activation, including MHC class II expression. This review also discusses the genetic basis of MHC class II expression and its implications for understanding MHC genotype associations with autoimmune diseases. Recent studies of interactions between endothelial cells and T cells are reviewed. Such interactions could be of major importance in the pathogenesis of autoimmune and vascular diseases.  N. Ref:: 217

 

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[77]

TÍTULO / TITLE:  - Valacyclovir provides optimum acyclovir exposure for prevention of cytomegalovirus and related outcomes after organ transplantation.

REVISTA / JOURNAL:  - J Infect Dis. Acceso gratuito al texto completo a partir de los 2 años de la publicación;  - http://www.journals.uchicago.edu/ 

      ●● Cita: J. of Infectious Diseases: <> 2002 Oct 15;186 Suppl 1:S110-5.

AUTORES / AUTHORS:  - Fiddian P; Sabin CA; Griffiths PD

INSTITUCIÓN / INSTITUTION:  - Royal Free and University College Medical School (Royal Free Campus), London NW3 2PF, United Kingdom. paul.fiddian@which.net

RESUMEN / SUMMARY:  - A meta-analysis of 12 randomized trials (1574 patients) examined herpesvirus and related outcomes following organ transplantation over a range of acyclovir exposures (including valacyclovir). Overall, cytomegalovirus (CMV) infection (odds ratio [OR], 0.44; 95% confidence interval [CI], 0.34-0.57; P<.001), CMV disease (OR, 0.41; 95% CI, 0.31-0.54; P<.001), death (OR, 0.60; 95% CI, 0.40-0.90; P=.01), opportunistic infection (OR, 0.70; 95% CI, 0.53-0.91; P=.009), acute graft rejection (OR, 0.67; 95% CI, 0.52-0.86; P<.001), herpes simplex virus disease (OR, 0.17; 95% CI, 0.12-0.24; P<.001), and varicella-zoster virus disease (OR, 0.06; 95% CI, 0.01-0.25; P<.001) were significantly reduced. Increased acyclovir exposure influenced more end points: Maximum efficacy resulted from valacyclovir (8 g/day). Increasing acyclovir exposure to that achieved with valacyclovir extends benefits of prophylaxis to include impact on graft rejection and opportunistic infections.

 

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[78]

TÍTULO / TITLE:  - Dendritic cells transduced with viral interleukin 10 or Fas ligand: no evidence for induction of allotolerance in vivo.

REVISTA / JOURNAL:  - Transplantation 2002 Jan 15;73(1 Suppl):S27-30.

AUTORES / AUTHORS:  - Buonocore S; Van Meirvenne S; Demoor FX; Paulart F; Thielemans K; Goldman M; Flamand V

INSTITUCIÓN / INSTITUTION:  - 2 Laboratory of Physiology, Medical School of Vrije Universiteit Brussel.

RESUMEN / SUMMARY:  - Dendritic cells (DC) are the most potent presenters of alloantigens and therefore are responsible for the induction of allograft rejection. Genetic modifications of DC allowing the expression of a tolerogenic molecule may render them immunosuppressive. We transduced bone marrow-derived DC with recombinant MFG retrovirus encoding either viral interleukin (vIL)-10 or Fas ligand (FasL) to induce transplantation tolerance. Up to 10 ng/ml of bioactive vIL-10 was produced by DC after transfer of the corresponding gene. Although the inhibitory properties of vIL-10-transduced DC were revealed in vitro in a mixed lymphocyte culture, no clear down-regulation of the allogeneic response was observed in vivo after single or multiple injections of those DC overexpressing vIL-10. When we transduced wild-type bone marrow-derived DC with recombinant MFG retrovirus encoding murine FasL, cells quickly died, probably because of suicidal or fratricidal Fas-dependent death. Indeed, only DC from Fas-deficient lpr mice survived to FasL gene transfer. Those FasL-transduced lpr DC exhibited a strong cytotoxic activity against Fas-positive targets in vitro. DC overexpressing FasL did not behave as immunosuppressive DC in vivo. The subcutaneous injection of FasL+ lpr DC in MHC class II-disparate mice hyperactivated the allospecific proliferation of T cells in the draining lymph nodes compared with mice treated with control-transduced DC. These results argue against the development of FasL+ DC or vIL-10-secreting DC as immunosuppressive tools in vivo. The alternative pathways of T-cell activation triggered by these genetically modified DC need to be investigated.  N. Ref:: 20

 

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[79]

TÍTULO / TITLE:  - Treatment of idiopathic nephrosis by immunophillin modulation.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2003 Aug;18 Suppl 6:vi79-86.

AUTORES / AUTHORS:  - Meyrier A

INSTITUCIÓN / INSTITUTION:  - Service de Nephrologie, Hopital Europeen Georges Pompidou, 20 rue Leblanc, F-75015 Paris, France. alain.meyrier@brs.ap-hop-paris.fr

RESUMEN / SUMMARY:  - Until 1985, glucocorticoids and cytotoxic drugs were the only treatments available for idiopathic nephrotic syndrome (nephrosis), that is, minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS). Trials of cyclosporine (CsA) treatment of nephrosis, the rationale of which was based on pathophysiologic considerations, have shown that this immunophillin modulator is effective in inducing and maintaining remission in patients suffering from idiopathic nephrotic syndrome. It appears that the best results, in the order of 80% remission rate, are obtained in steroid-sensitive cases, essentially MCD, and that in steroid-resistant FSGS the drug obtains remission in no more than 20% of the cases. Addition of glucocorticoids increases the success rate to approximately 30% of cases. Renal toxicity is proportional to previous impairment of renal function, primary renal disease (FSGS vs MCD) dosage >5.5 mg/kg/day and duration of treatment. The better bioavailability of the new formulation of CsA (Neoral), implies that the former dosage recommendations be reconsidered for distinctly lower figures. Repeat renal biopsy after 1 year of continuous CsA treatment is advisable, as stable serum creatinine levels may be falsely reassuring. CsA dependency is the rule during the first year of treatment. However, in some 25% of cases stable remission may be maintained after slow tapering off following 3-4 years of treatment. Other immunophillin modulators have been tried in the treatment of idiopathic nephrotic syndrome. Despite few preliminary reports indicating some success of tacrolimus the effects of this drug do not seem convincingly superior to CsA in terms of remission rate, toxicity and dependency. Rapamycin has not been tried in the treatment of nephrosis. Anecdotal cases of de novo FSGS induced by rapamycin in transplanted patients might indicate that this drug is in fact contraindicated in the treatment of nephrosis.  N. Ref:: 36

 

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[80]

TÍTULO / TITLE:  - In vitro generation of IL-10-producing regulatory CD4+ T cells is induced by immunosuppressive drugs and inhibited by Th1- and Th2-inducing cytokines.

REVISTA / JOURNAL:  - Immunol Lett 2003 Jan 22;85(2):135-9.

AUTORES / AUTHORS:  - O’Garra A; Barrat FJ

INSTITUCIÓN / INSTITUTION:  - Division of Immunoregulation, The National Institute for Medical Research (NIMR), The Ridgeway, Mill Hill, NW7 1AA, London, UK.  N. Ref:: 40

 

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[81]

TÍTULO / TITLE:  - Treating human autoimmune disease by depleting B cells.

REVISTA / JOURNAL:  - Ann Rheum Dis 2002 Oct;61(10):863-6.

AUTORES / AUTHORS:  - Looney RJ

INSTITUCIÓN / INSTITUTION:  - University of Rochester, Rochester, New York 14642, USA. John_looney@URMC.Rochester.edu  N. Ref:: 40

 

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[82]

TÍTULO / TITLE:  - The future of antigen-specific immunotherapy of allergy.

REVISTA / JOURNAL:  - Nat Rev Immunol 2002 Jun;2(6):446-53.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri824

AUTORES / AUTHORS:  - Valenta R

INSTITUCIÓN / INSTITUTION:  - Department of Pathophysiology, University of Vienna Medical School, Vienna General Hospital-AKH, Australia. Rudolf.valenta@akh-wein.ac.at

RESUMEN / SUMMARY:  - More than 25% of the population in industrialized countries suffers from immunoglobulin-E-mediated allergies. The antigen-specific immunotherapy that is in use at present involves the administration of allergen extracts to patients with the aim to cure allergic symptoms. However, the risk of therapy-induced side effects limits its broad application. Recent work indicates that the epitope complexity of natural allergen extracts can be recreated using recombinant allergens, and hypoallergenic derivatives of these can be engineered to increase treatment safety. It is proposed that these modified molecules will improve the current practice of specific immunotherapy and form a basis for prophylactic vaccination.  N. Ref:: 120

 

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[83]

TÍTULO / TITLE:  - Indoleamine 2,3-dioxygenase-expressing antigen-presenting cells and peripheral T-cell tolerance: another piece to the atopic puzzle?

REVISTA / JOURNAL:  - J Allergy Clin Immunol 2003 Nov;112(5):854-60.

      ●● Enlace al texto completo (gratuito o de pago) 1016/S0091

AUTORES / AUTHORS:  - von Bubnoff D; Hanau D; Wenzel J; Takikawa O; Hall B; Koch S; Bieber T

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Friedrich-Wilhelms University, Bonn, Germany.

RESUMEN / SUMMARY:  - There is growing evidence that dendritic cells, the major antigen-presenting cells and T-cell activators, have a broad effect on peripheral T-cell tolerance and regulation of immunity. Very recently, a new feature of regulatory antigen-presenting cells was observed. Certain dendritic cells, monocytes, and macrophages express the enzyme indoleamine 2,3-dioxygenase, and thus because of enhanced degradation of the essential amino acid tryptophan, they modulate T-cell activity in specific local tissue environments. In this review we discuss the various and apparently disparate effects of indoleamine 2,3-dioxygenase induction in cells of the immune system. We place current knowledge about this mechanism in the context of atopy. We introduce the hypothesis that tryptophan degradation might add to the ability to control and downregulate allergen-specific T-cell responses in atopic individuals.  N. Ref:: 44

 

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[84]

TÍTULO / TITLE:  - Cross-presentation in viral immunity and self-tolerance.

REVISTA / JOURNAL:  - Nat Rev Immunol 2001 Nov;1(2):126-34.

      ●● Enlace al texto completo (gratuito o de pago) 1038/35100512

AUTORES / AUTHORS:  - Heath WR; Carbone FR

INSTITUCIÓN / INSTITUTION:  - Immunology Division, The Walter and Eliza Hall Institute, Melbourne Hospital, Parkville, Victoria, Australia. heath@wehi.edu.au

RESUMEN / SUMMARY:  - T lymphocytes recognize peptide antigens presented by class I and class II molecules encoded by the major histocompatibility complex (MHC). Classical antigen-presentation studies showed that MHC class I molecules present peptides derived from proteins synthesized within the cell, whereas MHC class II molecules present exogenous proteins captured from the environment. Emerging evidence indicates, however, that dendritic cells have a specialized capacity to process exogenous antigens into the MHC class I pathway. This function, known as cross-presentation, provides the immune system with an important mechanism for generating immunity to viruses and tolerance to self.  N. Ref:: 83

 

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[85]

TÍTULO / TITLE:  - Scedosporium prolificans osteomyelitis in an immunocompetent child treated with voriconazole and caspofungin, as well as locally applied polyhexamethylene biguanide.

REVISTA / JOURNAL:  - J Clin Microbiol. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://jcm.asm.org/ 

      ●● Cita: J. Clinical Microbiology: <> 2003 Aug;41(8):3981-5.

AUTORES / AUTHORS:  - Steinbach WJ; Schell WA; Miller JL; Perfect JR

INSTITUCIÓN / INSTITUTION:  - Department of Pediatrics, Duke University, Durham, North Carolina 27710, USA. stein022@mc.duke.edu

RESUMEN / SUMMARY:  - Scedosporium species are increasingly isolated from immunocompromised and immunocompetent patients. Unfortunately, Scedosporium infections are generally resistant to amphotericin B, and Scedosporium prolificans strains are particularly resistant to the antifungal agents now in use. We report here on an immunocompetent child with S. prolificans-associated osteomyelitis successfully treated with debridement, local irrigation with polyhexamethylene biguanide, and the systemic administration of voriconazole and caspofungin despite poor in vitro activity of voriconazole alone against the isolate. We also review the treatments and outcomes of 28 reported cases of osteomyelitis or septic arthritis caused by Scedosporium species in immunocompetent patients.  N. Ref:: 62

 

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[86]

TÍTULO / TITLE:  - To DRiP or not to DRiP: generating peptide ligands for MHC class I molecules from biosynthesized proteins.

REVISTA / JOURNAL:  - Mol Immunol 2002 Oct;39(3-4):139-46.

AUTORES / AUTHORS:  - Yewdell J

INSTITUCIÓN / INSTITUTION:  - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Room 211 Bldg 4, 4 Center Drive, Bethesda, MD 20892-0440, USA. jyewdell@nih.gov  N. Ref:: 75

 

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[87]

TÍTULO / TITLE:  - Renal transplantation: can we reduce calcineurin inhibitor/stop steroids? Evidence based on protocol biopsy findings.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2003 Mar;14(3):755-66.

AUTORES / AUTHORS:  - Gotti E; Perico N; Perna A; Gaspari F; Cattaneo D; Caruso R; Ferrari S; Stucchi N; Marchetti G; Abbate M; Remuzzi G

INSTITUCIÓN / INSTITUTION:  - Department of Medicine and Transplantation, Ospedali Riuniti di Bergamo, Mario Negri Institute for Pharmacological Research, Italy.

RESUMEN / SUMMARY:  - How to combine antirejection drugs and which is the optimal dose of steroids and calcineurin inhibitors beyond the first year after kidney transplantation to maintain adequate immunosuppression without major side effects are far from clear. Kidney transplant patients on steroid, cyclosporine (CsA), and azathioprine were randomized to per-protocol biopsy (n = 30) or no-biopsy (n = 29) 1 to 2 yr posttransplant. Steroid or CsA were discontinued or reduced on the basis of biopsy to establish effects on drug-related complications, acute rejection, and graft function over 3 yr of follow-up. Serum creatinine, GFR (plasma clearance of iohexol), RPF (renal clearance of p-aminohippurate), CsA pharmacokinetics, and adverse events were monitored yearly. At the end, patients underwent a second biopsy. Per-protocol biopsy histology revealed no lesions (n = 5, steroid withdrawal), CsA nephropathy (n = 13, CsA discontinuation/reduction), or chronic rejection (n = 12, standard therapy). Reducing the drug regimen led to overall fewer side effects related to immunosuppression as compared with standard therapy or no-biopsy. Steroids were safely stopped with no acute rejection or graft loss. Complete CsA discontinuation was associated with acute rejection in the first four patients. Lowering CsA to low target CsA trough (30 to 70 ng/ml) never led to acute rejection or major renal function deterioration. Biopsy patients on conventional regimen had no acute rejection, one graft loss, no significant change in GFR, and significant RPF decline. No-biopsy controls: no acute rejection, one graft loss, significant decline of GFR and RPF. By serial biopsy analysis, severe lesions did not develop in patients with steroid discontinuation in contrast to patients on standard therapy over follow-up. CsA reduction did not adversely affect histology. Per-protocol biopsy more than 1 yr after kidney transplantation is a safe procedure to guide change of drug regimen and to lower the risk of major side effects.

 

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[88]

TÍTULO / TITLE:  - Clinical protocol. Purging of autologous stem cell sources with bcl-x(s) adenovirus for women undergoing high-dose chemotherapy for stage IV breast carcinoma.

REVISTA / JOURNAL:  - Hum Gene Ther 2001 Nov 1;12(16):2023-5.

AUTORES / AUTHORS:  - Ayash LJ; Clarke M; Adams P; Ferrara J; Ratanatharathorn V; Reynolds C; Roessler B; Silver S; Strawderman M; Uberti J; Wicha M

RESUMEN / SUMMARY:  - High-dose chemotherapy (HDCT) and autologous bone marrow transplantation (BMT) is frequently used to treat patients with metastatic cancer including breast cancer and neuroblastoma. However, the bone marrow of such patients is often contaminated with tumor cells. Recently, we have found that a recombinant adenovirus vector that contains a bcl-x, minigene (a dominant negative inhibitor of the bcl-2 family), called the bcl-x(s) adenovirus, is lethal to cancer cells derived from epithelial tissues, but not to normal human hematopoietic cells. To determine the mechanism, by which this virus spares normal hematopoietic cells, we isolated normal mouse hematopoietic stem cells and infected them with an adenovirus that contains a beta-galactosidase minigene. Such cells do not express beta-galactosidase, indicating that hematopoietic stem cells do not express transgene encoded by adenovirus vectors based upon the RSV-AD5 vector system. When breast cancer cells mixed with hematopoietic cells were infected with the bcl-x(s) adenovirus, cancer cells were selectively killed by the suicide adenoviruses. Hematopoietic cells exposed to the suicide vectors were able to reconstitute the bone marrow of mice exposed to lethal doses of y-irradiation. These studies suggest that adenovirus suicide vectors may provide a simple and effective method to selectively eliminate cancer cells derived from epithelial tissue that contaminate bone marrow to be used for autologous BMT. We therefore propose to initiate a phase I clinical trial to test the safety of this virus in women with breast cancer undergoing high does chemotherapy and autologous BMT.

 

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[89]

TÍTULO / TITLE:  - Regulation of gene expression in lymphocytes and antigen-presenting cells by measles virus: consequences for immunomodulation.

REVISTA / JOURNAL:  - J Mol Med 2002 Feb;80(2):73-85. Epub 2001 Nov 15.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00109-001-0299-x

AUTORES / AUTHORS:  - Schneider-Schaulies S; Bieback K; Avota E; Klagge I; ter Meulen V

INSTITUCIÓN / INSTITUTION:  - Institute for Virology and Immunobiology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany. s-s-s@vim.uni-wuerzburg.de

RESUMEN / SUMMARY:  - Acute measles, a well known disease usually contracted during early childhood, is still the major cause of vaccine-preventable infant deaths worldwide. There are about 40 million cases of acute measles per year, with more than one million cases of infant death as a consequence of measles. These are mainly due to opportunistic infections which develop on the basis of a generalized suppression of the cellular immunity in the course and after the acute disease. Lymphopenia, a general proliferative unresponsiveness of T cells ex vivo and cytokine imbalance, are considered as major hallmarks of measles virus (MV) induced immunosuppression. These findings are compatible with modulation of T cell responses by viral interference with professional antigen-presenting cells such as dendritic cells or direct effects on T cells by suppression of survival or proliferation signals. In vitro, MV interaction causes a variety of effects on dendritic cells, including maturation and loss of their allostimulatory functions. Whether there is an additional impact on the quality of T cell responses is unknown as yet. It is clear, however, that surface interaction of lymphocytes with the MV glycoprotein complex is necessary and sufficient to induce a state of proliferative unresponsiveness in T cells. This surface contact mediated signal essentially interferes with the propagation of the interleukin 2 receptor signal by blocking the activation of the protein kinase B, also called Akt kinase, both in vitro and after experimental infection.  N. Ref:: 122

 

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[90]

TÍTULO / TITLE:  - Mammalian target of rapamycin inhibition as therapy for hematologic malignancies.

REVISTA / JOURNAL:  - Cancer 2004 Feb 15;100(4):657-66.

      ●● Enlace al texto completo (gratuito o de pago) 1002/cncr.20026

AUTORES / AUTHORS:  - Panwalkar A; Verstovsek S; Giles FJ

INSTITUCIÓN / INSTITUTION:  - Section of Developmental Therapeutics, Department of Leukemia, University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA.

RESUMEN / SUMMARY:  - The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway, which mediates cell survival and proliferation. mTOR regulates essential signal-transduction pathways, is involved in the coupling of growth stimuli with cell cycle progression, and initiates mRNA translation in response to favorable nutrient environments. mTOR is involved in regulating many aspects of cell growth, including membrane traffic, protein degradation, protein kinase C signaling, ribosome biogenesis, and transcription. Because mTOR activates both the 40S ribosomal protein S6 kinase (p70s6k) and the eukaryotic initiation factor 4E-binding protein 1, its inhibitors cause G1-phase cell cycle arrest. Inhibitors of mTOR also prevent cyclin dependent kinase (CDK) activation, inhibit retinoblastoma protein phosphorylation, and accelerate the turnover of cyclin D1, leading to a deficiency of active CDK4/cyclin D1 complexes, all of which may help cause G1-phase arrest. It is known that the phosphatase and tensin homologue tumor suppressor gene (PTEN) plays a major role in embryonic development, cell migration, and apoptosis. Malignancies with PTEN mutations, which are associated with constitutive activation of the PI3K/Akt pathway, are relatively resistant to apoptosis and may be particularly sensitive to mTOR inhibitors. Rapamycin analogs with relatively favorable pharmaceutical properties, including CCI-779, RAD001, and AP23573, are under investigation in patients with hematologic malignancies.  N. Ref:: 116

 

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[91]

TÍTULO / TITLE:  - Cholesteryl ester transfer protein facilitates the movement of water-insoluble drugs between lipoproteins: a novel biological function for a well-characterized lipid transfer protein.

REVISTA / JOURNAL:  - Biochem Pharmacol 2002 Dec 15;64(12):1669-75.

AUTORES / AUTHORS:  - Kwong M; Wasan KM

INSTITUCIÓN / INSTITUTION:  - Division of Pharmaceutics and Biopharmaceutics, Faculty of Pharmaceutical Sciences, The University of British Columbia, 2146 East Mall Avenue, Vancouver, BC, Canada V6T 1Z3.

RESUMEN / SUMMARY:  - This review article addresses the recently discovered finding that cholesteryl ester transfer protein (CETP) can facilitate the transfer of water-insoluble drugs between different lipoprotein subclasses. This protein, which is often referred to as lipid transfer protein I (LTP I), is involved in the lipid regulation of lipoproteins. It is responsible for the facilitated transfer of core lipoprotein lipids, cholesteryl ester and triglycerides, and approximately one-third of the coat lipoprotein lipid, phosphatidylcholine, between different plasma lipoproteins. The human body appears to recognize exogenous water-insoluble drugs as lipid-like particles, which suggests that these compounds may interact with lipoproteins just like endogenous plasma lipids, and thus their transfer between lipoproteins may be facilitated by plasma CETP. Patients with a variety of diseases (i.e. diabetes, cancer, AIDS) often exhibit hypo- and/or hypercholesterolemia and triglyceridemia, commonly referred to as dyslipidemias, which result in changes in their plasma lipoprotein-lipid composition and concentration. The interaction of water-insoluble drugs with these dyslipidemic lipoproteins may be responsible for the differences seen in the pharmacokinetics and pharmacodynamics of the drug within different diseased patient populations. It is possible that these differences may be linked to the ability of CETP to transfer these compounds from one lipoprotein to another. This review examines the current understanding of the relationship between CETP activity and the lipoprotein distribution of a number of compounds (e.g. amphotericin B and cyclosporine A). It further suggests that additional research will expand our understanding of the role of CETP to explain other functions in lipophilic drug distribution and metabolism.  N. Ref:: 45

 

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[92]

TÍTULO / TITLE:  - Heme oxygenase in liver transplantation: heme catabolism and metabolites in the search of function.

REVISTA / JOURNAL:  - Hepatology 2003 Aug;38(2):286-8.

      ●● Enlace al texto completo (gratuito o de pago) 1053/jhep.2003.50360

AUTORES / AUTHORS:  - Bauer M  N. Ref:: 31

 

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[93]

TÍTULO / TITLE:  - Clinical development of mammalian target of rapamycin inhibitors.

REVISTA / JOURNAL:  - Hematol Oncol Clin North Am 2002 Oct;16(5):1101-14.

AUTORES / AUTHORS:  - Dancey JE

INSTITUCIÓN / INSTITUTION:  - Cancer Treatment Evaluation Program, Division of Cancer Treatment and Diagnosis, Investigational Drug Branch/CTEP/DCTD/NCI, 6130 Executive Boulevard, EPN 7131, Rockville, MD 20854, USA. danceyj@ctep.nci.nih.gov

RESUMEN / SUMMARY:  - Rapamycin and CCI-779 have significant in vitro and in vivo anti-proliferative activity against a broad range of human tumor cell lines, justifying the clinical evaluation of this class of agent in cancer patients. Preliminary results from phase I studies of CCI-779 suggest that the agent is well tolerated and has anti-tumor activity. The challenge to investigators is to efficiently determine what role this class of agent will play in the treatment of cancer patients.  N. Ref:: 69

 

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[94]

TÍTULO / TITLE:  - Regulatory T cells in kidney transplant recipients: active players but to what extent?

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2003 Jun;14(6):1706-8.

AUTORES / AUTHORS:  - Zhai Y; Kupiec-Weglinski JW  N. Ref:: 20

 

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[95]

TÍTULO / TITLE:  - The bare lymphocyte syndrome and the regulation of MHC expression.

REVISTA / JOURNAL:  - Annu Rev Immunol 2001;19:331-73.

      ●● Enlace al texto completo (gratuito o de pago) 1146/annurev.immunol.19.1.331

AUTORES / AUTHORS:  - Reith W; Mach B

INSTITUCIÓN / INSTITUTION:  - Jeantet Laboratory of Molecular Genetics, Department of Genetics and Microbiology, University of Geneva Medical School, 1 rue Michel-Servet, Geneva 4, 1211 Switzerland. Walter.Reith@medecine.unige.ch

RESUMEN / SUMMARY:  - The bare lymphocyte syndrome (BLS) is a hereditary immunodeficiency resulting from the absence of major histocompatibility complex class II (MHCII) expression. Considering the central role of MHCII molecules in the development and activation of CD4(+) T cells, it is not surprising that the immune system of the patients is severely impaired. BLS is the prototype of a “disease of gene regulation.” The affected genes encode RFXANK, RFX5, RFXAP, and CIITA, four regulatory factors that are highly specific and essential for MHCII genes. The first three are subunits of RFX, a trimeric complex that binds to all MHCII promoters. CIITA is a non-DNA-binding coactivator that functions as the master control factor for MHCII expression. The study of RFX and CIITA has made major contributions to our comprehension of the molecular mechanisms controlling MHCII genes and has made this system into a textbook model for the regulation of gene expression.  N. Ref:: 183

 

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[96]

TÍTULO / TITLE:  - Prediction of an HLA-DR-binding peptide derived from Wilms’ tumour 1 protein and demonstration of in vitro immunogenicity of WT1(124-138)-pulsed dendritic cells generated according to an optimised protocol.

REVISTA / JOURNAL:  - Cancer Immunol Immunother 2002 Jul;51(5):271-81. Epub 2002 Apr 26.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00262-002-0278-2

AUTORES / AUTHORS:  - Knights AJ; Zaniou A; Rees RC; Pawelec G; Muller L

INSTITUCIÓN / INSTITUTION:  - University of Tubingen, Section for Transplantation Immunology and Immunohaematology, Second Department of Internal Medicine, Zentrum fur Medizinische Forschung ZMF, Waldhornlestrasse 22, 72072 Tubingen, Germany.

RESUMEN / SUMMARY:  - The Wilms’ tumour 1 (WT1) protein is over-expressed in several types of cancer including leukaemias and might therefore constitute a novel target for immunotherapy. Recently, human leucocyte antigen (HLA) class I-binding WT1 peptides have been identified and shown to stimulate CD8(+) T cells in vitro. For maximal CD8 cell efficacy, CD4(+) helper T cells responding to major histocompatibility complex (MHC) class II-binding epitopes are required. Here, we report that scanning the WT1 protein sequence using an evidence-based predictive computer algorithm (SYFPEITHI) yielded a peptide WT1(124-138) predicted to bind the HLA-DRB1*0401 molecule with high affinity. Moreover, synthetic WT1(124-138)-peptide-pulsed dendritic cells (DC), generated according to a protocol optimised in the present study, sensitised T cells in vitro to proliferate and secrete interferon-gamma (IFN-gamma) when rechallenged with specific peptide-pulsed DC, but not with peripheral blood mononuclear cells (PBMC). These results suggest that the WT1 protein may yield epitopes immunogenic to CD4 as well as CD8 T cells, and therefore constitute a novel potential target for specific immunotherapy.

 

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[97]

TÍTULO / TITLE:  - Induction of T cell alertness by bacterial colonization of intestinal epithelium.

REVISTA / JOURNAL:  - Proc Natl Acad Sci U S A. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.pnas.org/ 

      ●● Cita: Proc Natl Acad Sci USA (PNAS): <> 2002 Mar 5;99(5):2584-6.

      ●● Enlace al texto completo (gratuito o de pago) 1073/pnas.062058399

AUTORES / AUTHORS:  - Spies T

INSTITUCIÓN / INSTITUTION:  - Fred Hutchinson Cancer Research Center, Clinical Research Division, 1100 Fairview Avenue North, Seattle, WA 98109, USA. tspies@fhcrc.org  N. Ref:: 32

 

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[98]

TÍTULO / TITLE:  - Interleukin 2 receptor antagonists for kidney transplant recipients.

REVISTA / JOURNAL:  - Cochrane Database Syst Rev 2004;1:CD003897.

      ●● Enlace al texto completo (gratuito o de pago) 1002/14651858.CD003897.pub2

AUTORES / AUTHORS:  - Webster A; Playford E; Higgins G; Chapman J; Craig J

INSTITUCIÓN / INSTITUTION:  - Centre for Kidney Research, The Children’s Hospital at Westmead, Locked Bag 4001, Westmead, NSW, AUSTRALIA, 2145.

RESUMEN / SUMMARY:  - BACKGROUND: Interleukin 2 receptor antagonists (IL2Ra) are used as induction therapy for prophylaxis against acute rejection in kidney transplant recipients. Use of IL2Ra has increased steadily, with 38% of new kidney transplant recipients in the United States, and 23% in Australasia receiving IL2Ra in 2002. OBJECTIVES: This study aims to systematically identify and summarise the effects of using an IL2Ra, as an addition to standard therapy, or as an alternative to other antibody therapy. SEARCH STRATEGY: The Cochrane Renal Group’s specialised register (June 2003), the Cochrane Controlled Trials Register (in The Cochrane Library issue 3, 2002), MEDLINE (1966-November 2002) and EMBASE (1980-November 2002). Reference lists and abstracts of conference proceedings and scientific meetings were hand-searched from 1998-2003. Trial groups, authors of included reports and drug manufacturers were contacted. SELECTION CRITERIA: Randomised controlled trials (RCTs) in all languages comparing IL2Ra to placebo, no treatment, other IL2Ra or other antibody therapy. DATA COLLECTION AND ANALYSIS: Data was extracted and quality assessed independently by two reviewers, with differences resolved by discussion. Dichotomous outcomes are reported as relative risk (RR) with 95% confidence intervals (CI). MAIN RESULTS: One hundred and seventeen reports from 38 trials involving 4893 participants were included. Where IL2Ra were compared with placebo (17 trials; 2786 patients), graft loss was not significantly different at one (RR 0.83, 95% CI 0.66 to 1.04) or three years (RR 0.88, 95% CI 0.64 to 1.22). Acute rejection (AR) was significantly reduced at six months (RR 0.66, 95% CI 0.59 to 0.74) and at one year (RR 0.67, 95% CI 0.60 to 0.75). At one year, cytomegalovirus (CMV) infection (RR 0.82, 95% CI 0.65 to 1.03) and malignancy (RR 0.67, 95% CI 0.33 to 1.36) were not significantly different. Where IL2Ra were compared with other antibody therapy no significant differences in treatment effects were demonstrated, but adverse effects strongly favoured IL2Ra. REVIEWER’S CONCLUSIONS: Given a 40% risk of rejection, seven patients would need treatment with IL2Ra to prevent one patient having rejection, with no definite improvement in graft or patient survival. There is no apparent difference between basiliximab and daclizumab. IL2Ra are as effective as other antibody therapies and with significantly fewer side effects

 

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[99]

TÍTULO / TITLE:  - Treatment of nephrotic syndrome in children and controlled trials.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2003 Aug;18 Suppl 6:vi75-8.

AUTORES / AUTHORS:  - Filler G

INSTITUCIÓN / INSTITUTION:  - Department of Paediatrics, Division of Nephrology, Children’s Hospital of Eastern Ontario, University of Ottawa, Canada. filler@cheo.on.ca

RESUMEN / SUMMARY:  - AIM: To determine the sequential therapy of childhood nephrotic syndrome (NS) with presumed minimal change nephropathy using the evidence from clinical trials. METHODS: Meta-analysis of 22 randomized controlled trials was performed, using frequency of relapse and side effects of therapeutic regimes. RESULTS: A meta-analysis of seven trials comparing duration of therapy for initial onset showed that duration of at least 3 months significantly reduced the risk of relapse at 12-24 months (relative risk 0.73; 95% confidence interval 0.60-0.89) without an increase in adverse events. Five trials were performed for steroid treatment of relapse. Deflazacort reduced relapses during therapy, but is not generally available. No difference was observed when comparing single and divided dosing of prednisone. Frequency of relapses could not be influenced by duration of relapse therapy. Alternate day therapy was more effective than intermittent use of prednisone. Two studies out of five on cyclophosphamide or chlorambucil showed consistently that alkylating agents should be used before cyclosporine as alternative therapy to steroids. CONCLUSIONS: Children with initial onset of NS should be treated with prednisone at a dose of 60 mg/m(2)/day for 6 weeks, followed by a dose of 40 mg/m(2)/48 h for at least another 6 weeks. If steroid toxicity for treatment of relapsing NS requires alternative treatment, cyclophosphamide (2 mg/kg/day for at least 8 weeks) remains the drug of choice with a curative potential. If children still relapse after alkylating agents, levamisole may serve as an alternative only for frequent relapsing NS, whereas steroid-dependent NS should be treated with cyclosporine.

 

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[100]

TÍTULO / TITLE:  - Interventions for bullous pemphigoid.

REVISTA / JOURNAL:  - Cochrane Database Syst Rev 2003;(3):CD002292.

      ●● Enlace al texto completo (gratuito o de pago) 1002/14651858.CD002292

AUTORES / AUTHORS:  - Khumalo N; Kirtschig G; Middleton P; Hollis S; Wojnarowska F; Murrell D

INSTITUCIÓN / INSTITUTION:  - Dermatology Department, Groote Schuur Hospital, Cape Town, South Africa, Anzio Road, Observatory, Cape Town, Western Cape, South Africa.

RESUMEN / SUMMARY:  - BACKGROUND: Bullous pemphigoid is the most common autoimmune bullous disease in the West. Oral steroids are considered the standard treatment. OBJECTIVES: To assess the effects of treatments for bullous pemphigoid. SEARCH STRATEGY: We searched the Skin Group Specialised Register, the Cochrane Central Register of Controlled Trials, MEDLINE, EMBASE to March 2003 and bibliographies from identified studies. SELECTION CRITERIA: Randomised controlled trials of treatments for patients with immunofluorescence confirmed bullous pemphigoid. DATA COLLECTION AND ANALYSIS: Two reviewers evaluated the studies in terms of the inclusion criteria, five extracted data independently; disagreements were resolved by discussion. Statistical pooling of the data was inappropriate because of heterogeneity of treatments. MAIN RESULTS: We found seven randomised controlled trials with a total of 634 patients. All studies involved different comparisons, none included a placebo group.Different doses, different formulations of corticosteroids and the addition of azathioprine failed to show significant differences in measures of disease control. However, patients who took azathioprine were able to almost halve the amount of prednisone required for disease control. Plasma exchange plus prednisone achieved significantly better disease control than prednisone alone; this favourable effect was not apparent in another study. The latter study also compared plasma exchange or azathioprine plus prednisone, but failed to show significant differences for disease control or mortality, although total adverse events at six months almost reached statistical significance in favour of plasma exchange plus prednisone. Comparing tetracycline plus nicotinamide with prednisolone, no significant difference for disease response was shown. A very potent topical corticosteroid was compared to oral prednisone in patients with moderate and extensive disease. In patients with extensive disease, the topical steroid group showed significantly better survival and disease control, and less severe complications, while no significant differences for these outcomes were seen in patients with moderate disease.Most of the reported deaths were in patients taking high doses of oral corticosteroids. REVIEWER’S CONCLUSIONS: Very potent topical steroids are effective and safe treatments for bullous pemphigoid; their use in extensive disease may be limited by side effects and practical factors.Starting doses of prednisolone greater than 0.75 mg/kg/day do not seem to give additional benefit, lower doses may be adequate for disease control; this could reduce the incidence and severity of adverse reactions.The effectiveness of the addition of plasma exchange or azathioprine to corticosteroids has not been established.Combination treatment with tetracycline and nicotinamide may be useful; this needs further validation.  N. Ref:: 38

 

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[101]

TÍTULO / TITLE:  - Pumping iron: the strange partnership of the hemochromatosis protein, a class I MHC homolog, with the transferrin receptor.

REVISTA / JOURNAL:  - Traffic 2001 Mar;2(3):167-74.

AUTORES / AUTHORS:  - Enns CA

INSTITUCIÓN / INSTITUTION:  - Department of Cell and Developmental Biology, L-215, Oregon Health Sciences University, Portland, OR 97201, USA. ennsca@ohsu.edu

RESUMEN / SUMMARY:  - People suffering from hereditary hemochromatosis (HH) can not regulate the uptake of iron properly and gradually accumulate iron in their body over their lifetime. The protein involved in HH, HFE, has been recently identified as a class I major histocompatibility complex (MHC) homolog. The wild-type HFE associates and co-traffics with the transferrin receptor (TfR). The mutation responsible for 83% of HH (C260Y) results in the failure of HFE to form a critical disulfide bond, bind beta2 microglobulin, bind TfR, and traffic to the cell surface. In non-polarized cells, the partnership of HFE and TfR results in decreased iron uptake into cells. The mechanism whereby a class I MHC homolog modifies the function of a membrane receptor and how this dynamic complex of molecules regulates iron transport across intestinal epithelial cells is the subject of this review.  N. Ref:: 66

 

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[102]

TÍTULO / TITLE:  - Nutritional pharmacology in surgical patients.

REVISTA / JOURNAL:  - Am J Surg 2002 Apr;183(4):349-52.

AUTORES / AUTHORS:  - Alexander JW

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Cincinnati, Ohio 45267-2558, USA. jwesley.alexander@uc.edu

RESUMEN / SUMMARY:  - The use of pharmaconutrition for supportive care of surgical patients is now well established, but the field is still in its infancy. Complex pharmaconutrient formulas containing arginine, glutamine, and n-3 fatty acids have been proven to shorten hospital stay, decrease the incidence of infection, and reduce hospital costs in selected groups of patients. The effects are greatest in those patients with severe trauma including burn injury, those undergoing major surgical procedures, especially when malnourished, and those who are critically ill ICU patients including patients with existing infection. The complex interaction of pharmaconutrients and other pharmacologic agents are just now beginning to be investigated.  N. Ref:: 22

 

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[103]

TÍTULO / TITLE:  - IL-10 and its related cytokines for treatment of inflammatory bowel disease.

REVISTA / JOURNAL:  - World J Gastroenterol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.wjgnet.com/1007-9327/wj.htm 

      ●● Cita: World Journal of Gastroenterology: <> 2004 Mar 1;10(5):620-5.

AUTORES / AUTHORS:  - Li MC; He SH

INSTITUCIÓN / INSTITUTION:  - Allergy and Inflammation Research Institute, Shantou University Medical College, 22 Xin Ling Road, Shantou 515041, Guangdong Province, China.

RESUMEN / SUMMARY:  - Inflammatory bowel diseases (IBDs), including Crohn’s disease and ulcerative colitis are chronic inflammatory disorders of gastrointestinal tract. Although the etiology is incompletely understood, initiation and aggravation of the inflammatory process seem to be due to a massive local mucosal immune response. Interleukin-10 (IL-10) is a regulatory cytokine which inhibits both antigen presentation and subsequent pro-inflammatory cytokine release, and it is proposed as a potent anti-inflammatory biological therapy in chronic IBD. Many methods of IL-10 as a treatment for IBD have been published. The new strategies of IL-10 treatment, including recombinant IL-10, the use of genetically modified bacteria, gelatine microsphere containing IL-10, adenoviral vectors encoding IL-10 and combining regulatory T cells are discussed in this review. The advantages and disadvantages of these IL-10 therapies are summarized. Although most results of recombinant IL-10 therapies are disappointing in clinical testing because of lacking efficacy or side effects, therapeutic strategies utilizing gene therapy may enhance mucosal delivery and increase therapeutic response. Novel IL-10-related cytokines, including IL-19, IL-20, IL-22, IL-24, IL-26, IL-28 and IL-29, are involved in regulation of inflammatory and immune responses. The use of IL-10 and IL-10-related cytokines will provide new insights into cell-based and gene-based treatment against IBD in near future.  N. Ref:: 54

 

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[104]

TÍTULO / TITLE:  - Potential role of major histocompatibility complex class II peptides in regulatory tolerance to vascularized grafts.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 15;77(1 Suppl):S35-7.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000106472.91343.8D

AUTORES / AUTHORS:  - LeGuern C

INSTITUCIÓN / INSTITUTION:  - Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA. leguern@helix.mgh.harvard.edu

RESUMEN / SUMMARY:  - The inactivation of persisting T lymphocytes reactive to self- and non-self-antigens is a major arm of operational immune tolerance in mammals. Silencing of such T cells proceeds mostly by means of suppression, a process that is mediated by regulatory T-cell subsets and especially by CD4(+)CD(25high) regulatory T cells (Treg). Although Treg activation and ensuing suppressive activity appear to be major histocompatibility complex class II dependent, the fine specificity of Treg T-cell receptors has not yet been elucidated. Recent data from the author’s laboratory on a class II gene therapy induction of tolerance to allogeneic kidney grafts suggest that class II peptides are involved as generic signals for Treg activation. A brief compilation of results that would support this hypothesis is discussed in the present article.  N. Ref:: 31

 

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[105]

TÍTULO / TITLE:  - Natural killer cell receptors: new biology and insights into the graft-versus-leukemia effect.

REVISTA / JOURNAL:  - Blood. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.bloodjournal.org/ 

      ●● Cita: Blood: <> 2002 Sep 15;100(6):1935-47.

      ●● Enlace al texto completo (gratuito o de pago) 1182/blood-2002-02-0350

AUTORES / AUTHORS:  - Farag SS; Fehniger TA; Ruggeri L; Velardi A; Caligiuri MA

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine, Division of Hematology/Oncology, The Ohio State University, A433A Starling Loving Hall, 320 W Tenth Avenue, Columbus, OH 43210, USA. farag-1@medctr.osu.edu

RESUMEN / SUMMARY:  - Natural killer (NK) cells have held great promise for the immunotherapy of cancer for more than 3 decades. However, to date only modest clinical success has been achieved manipulating the NK cell compartment in patients with malignant disease. Progress in the field of NK cell receptors has revolutionized our concept of how NK cells selectively recognize and lyse tumor and virally infected cells while sparing normal cells. Major families of cell surface receptors that inhibit and activate NK cells to lyse target cells have been characterized, including killer cell immunoglobulinlike receptors (KIRs), C-type lectins, and natural cytotoxicity receptors (NCRs). Further, identification of NK receptor ligands and their expression on normal and transformed cells completes the information needed to begin development of rational clinical approaches to manipulating receptor/ligand interactions for clinical benefit. Indeed, clinical data suggest that mismatch of NK receptors and ligands during allogeneic bone marrow transplantation may be used to prevent leukemia relapse. Here, we review how NK cell receptors control natural cytotoxicity and novel approaches to manipulating NK receptor-ligand interactions for the potential benefit of patients with cancer.  N. Ref:: 134

 

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[106]

TÍTULO / TITLE:  - Donor chimerism and stem cell function in a murine congenic transplantation model after low-dose radiation conditioning: effects of a retroviral-mediated gene transfer protocol and implications for gene therapy.

REVISTA / JOURNAL:  - Exp Hematol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.medicinedirect.com/journal 

      ●● Cita: Experimental Hematology: <> 2002 Nov;30(11):1324-32.

AUTORES / AUTHORS:  - Goebel WS; Yoder MC; Pech NK; Dinauer MC

INSTITUCIÓN / INSTITUTION:  - Herman B. Wells Center for Pediatric Research and Department of Pediatrics, Hematology/Oncology, James Whitcomb Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, IN, USA.

RESUMEN / SUMMARY:  - OBJECTIVE: We investigated low-dose radiation conditioning for the transplantation of retrovirus-transduced cells in a C57Bl6/J murine model. MATERIALS AND METHODS: The effect of low-dose radiation on stem cell function was investigated using a competitive repopulation assay. Stem cell function of marrow cells that underwent a retroviral-mediated gene transfer (RMGT) protocol was examined by this assay, and donor chimerism of these cells when transplanted into 160-cGy conditioned syngeneic hosts was compared to fresh marrow. RESULTS: Irradiation with 300 or 160 cGy substantially decreased stem cell function as measured by competitive repopulation. Animals conditioned with 160 cGy and transplanted with 20 x 10(6) fresh marrow cells permitted donor cell engraftment of 53.6% +/- 11.4% 6 months after transplant compared to 100% donor cell engraftment after 1100 cGy irradiation. Lymphoid and myeloid engraftment did not significantly differ from total engraftment in submyeloablated hosts. When transplanted into lethally irradiated hosts, the competitive repopulating activity of marrow treated with a single dose of 5-fluorouracil followed by ex vivo culture according to a standard RMGT protocol was equal to 5-fluorouracil-only treated marrow. However, cells treated with 5-fluorouracil or 5-fluorouracil plus ex vivo culture for RMGT repopulated less well than fresh marrow cells in 160 cGy conditioned hosts. CONCLUSIONS: Low-dose irradiation decreases host stem cell function, allowing engraftment of both fresh and RMGT protocol-treated marrow, although the engraftment of 5-fluorouracil-treated cells was reduced at least two-fold, and 5-fluorouracil plus RMGT protocol-treated cells at least three-fold, compared to fresh marrow. Modification of current RMGT protocols may be important for optimizing engraftment under these conditions.

 

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[107]

TÍTULO / TITLE:  - T-cell activation through the antigen receptor. Part 2: role of signaling cascades in T-cell differentiation, anergy, immune senescence, and development of immunotherapy.

REVISTA / JOURNAL:  - J Allergy Clin Immunol 2002 Jun;109(6):901-15.

AUTORES / AUTHORS:  - Nel AE; Slaughter N

INSTITUCIÓN / INSTITUTION:  - Division of Clinical Immunology/Allergy, Department of Medicine, UCLA School of Medicine, University of California, Los Angeles 90095-1680, USA.

RESUMEN / SUMMARY:  - Part 2 of this review on cellular activation by the T-cell antigen receptor (TCR) will highlight how TCR signaling pathways are adapted to achieve specific biologic outcomes, including different states of T-cell differentiation and the induction of T-cell tolerance. We will also explore how treatment with altered peptide ligands affects TCR signaling to change T-cell differentiation or to induce an anergy state. These changes are accomplished through alteration of protein tyrosine kinase activity, the stoichiometry of phosphorylation of immunoreceptor tyrosine-based activation motifs, intracellular free ionized calcium flux, mitogen-activated protein kinase activity, and transcriptional activation of key cytokine promoters. The CTLA-4 plays an important role in the induction and maintenance of anergy. The second theme will highlight how altered TCR signal transduction, including changes in the compartmentalization of signaling components at the TCR synapse, contributes to decreased T-cell activation during immune senescence. Finally, we will illustrate how the molecular details of TCR activation can be used to modify the function of the immune system. This includes a description of the mechanism of action of altered peptide ligands, CTLA-4Ig, and pharmacologic inhibitors of mitogen-activated protein kinases, nuclear factor kappaB, and protein kinase C cascades.  N. Ref:: 128

 

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[108]

TÍTULO / TITLE:  - HLA in coeliac disease: unravelling the complex genetics of a complex disorder.

REVISTA / JOURNAL:  - Tissue Antigens 2003 Feb;61(2):105-17.

AUTORES / AUTHORS:  - Louka AS; Sollid LM

INSTITUCIÓN / INSTITUTION:  - Institute of Immunology, Rikshospitalet, University of Oslo, Norway.

RESUMEN / SUMMARY:  - Coeliac disease (gluten sensitive enteropathy) is a common, polygenic and multifactorial disorder that serves as a pioneering model for the study of inflammatory disease. A major environmental factor is known (ingested gluten from wheat), and there is unprecedented genetic and functional evidence pinpointing HLA-DQA1*05-DQB1*02 ( DQ2) and DQA1*03-DQB1*0302 ( DQ8) in disease predisposition. We discuss the current state of play in coeliac disease genetics, focussing particularly on the HLA complex. Emerging evidence suggests that additional HLA risk loci exert weak effects, independent of DQA1*05-DQB1*02, on the B8-DR3-DQ2 haplotype. There is also good evidence from linkage studies of disease gene(s) on chromosome 5q. We discuss the role and implications of linkage disequilibrium and haplotype blocks in complex disease gene mapping. We briefly address findings from studies of animal models for chronic inflammatory disease, and consider roles for both common genes associated with multiple inflammatory diseases, and genes unique to coeliac disease. The coeliac genetics research community has established a sound foundation for the identification of additional disease genes in the not-too-distant future. Functional studies will play a critical role, and coeliac disease has a promising future in this respect. Coeliac disease continues to function as a model disorder, facilitating the development and implementation of complex disease gene mapping strategies.  N. Ref:: 59

 

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[109]

TÍTULO / TITLE:  - HLA-DPB1 and chronic beryllium disease: a HuGE review.

REVISTA / JOURNAL:  - Am J Epidemiol 2003 Mar 1;157(5):388-98.

AUTORES / AUTHORS:  - McCanlies EC; Kreiss K; Andrew M; Weston A

INSTITUCIÓN / INSTITUTION:  - Biostatistics and Epidemiology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA. eim4@cdc.gov

RESUMEN / SUMMARY:  - The human leukocyte antigen (HLA) complex is a series of genes located on chromosome 6 that are important in normal immune function. Susceptibility to chronic beryllium disease, a granulomatous lung disease that appears in workers exposed to beryllium, is modified by genetic variants of the HLA-DP subregion. Evaluation of HLA-DPB1 sequence motifs in current and former beryllium workers implicated a glutamic acid residue at position 69 (HLA-DPB1(Glu69)) in chronic beryllium disease. This finding has since been extended to specific HLA-DPB1(Glu69) alleles. Specific job tasks have also been implicated in degree of risk, and in this paper the authors explore gene-environment interaction. The utility of this genetic information for prospective, current, and former beryllium workers must be weighed against the potential for employment and insurance discrimination. Continued research in the beryllium-exposed population will be important for improving personal risk assessment and identifying high-risk genes associated with disease progression.  N. Ref:: 79

 

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[110]

TÍTULO / TITLE:  - Donor-specific tolerance in fully major histocompatibility major histocompatibility complex-mismatched limb allograft transplants under an anti-alphabeta T-cell receptor monoclonal antibody and cyclosporine A protocol.

REVISTA / JOURNAL:  - Transplantation 2003 Dec 27;76(12):1662-8.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000105343.49626.6F

AUTORES / AUTHORS:  - Siemionow MZ; Izycki DM; Zielinski M

INSTITUCIÓN / INSTITUTION:  - Department of Plastic Surgery, Cleveland Clinic Foundation, A60, 9500 Euclid Avenue, Cleveland, OH 44195, USA. siemiom@ccf.org

RESUMEN / SUMMARY:  - BACKGROUND: Recent studies have demonstrated that treatment with alphabeta-T-cell receptor (TCR) monoclonal antibody and cyclosporine A (CsA) can extend survival in composite tissue allografts (CTA). The purpose of this study was to induce tolerance in fully major histocompatibility complex (MHC)-mismatched rat limb allografts under 7 days of a combined alphabeta-TCR-CsA protocol. METHODS: The authors performed 30 hind-limb allotransplantations across the MHC barrier between Brown Norway donors (BN; RT1n) and Lewis recipients (LEW; RT1l). Isograft and allograft controls received no treatment. The experimental groups received monotherapy of alphabeta-TCR and CsA or a combination of alphabeta-TCR and CsA for 7 days only. Donor-specific tolerance and immunocompetence were determined by standard skin grafting in vivo and mixed lymphocyte reaction (MLR) in vitro. The efficacy of immunosuppressive therapy and the level of donor-specific chimerism were determined by flow cytometry. RESULTS: Long-term survival (>350 days) was achieved in allograft recipients (n=6) under the 7-day protocol of combined alphabeta-TCR-CsA. Donor-specific tolerance and immunocompetence of long-term chimeras were confirmed by acceptance of skin grafts from the donors and rejection of the third-party alloantigens (AxC Irish). At day 120, MLR demonstrated unresponsiveness to the host and donor antigens but strong reactivity against third-party alloantigens. Flow cytometry confirmed the high efficacy of immunosuppressive treatment and the development of donor-specific chimerism (7.6% of CD4+-RT1n+ cells, 1.3% of CD8+-RT1n+ cells, and 16.5% of CD45RA+-RT1n+ cells) in the periphery of tolerated recipients. CONCLUSIONS: Combined therapy of alphabeta-TCR-CsA for 7 days resulted in tolerance induction in fully MHC-mismatched rat hind-limb allografts. Tolerance was directly associated with stable, donor-specific chimerism.

 

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[111]

TÍTULO / TITLE:  - Perioperative single-dose glucocorticoid administration: pathophysiologic effects and clinical implications.

REVISTA / JOURNAL:  - J Am Coll Surg 2002 Nov;195(5):694-712.

AUTORES / AUTHORS:  - Holte K; Kehlet H

INSTITUCIÓN / INSTITUTION:  - Department of Surgical Gastroenterology, Hvidovre University Hospital, Denmark.  N. Ref:: 138

 

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[112]

TÍTULO / TITLE:  - The genetics of autoimmune endocrine disease.

REVISTA / JOURNAL:  - Clin Endocrinol (Oxf) 2003 Jul;59(1):1-11.

AUTORES / AUTHORS:  - Tait KF; Gough SC

INSTITUCIÓN / INSTITUTION:  - Division of Medical Sciences, University of Birmingham, Edgbaston and Birmingham Heartlands Hospital, Bordesley Green East, Birmingham, UK.

RESUMEN / SUMMARY:  - The common autoimmune endocrinopathies result from an interaction between environmental factors and genetic predisposition. Several chromosomal gene regions have been shown to contribute to more than one disease, supporting the clinical observation that the autoimmune endocrine diseases cluster within individuals and families. Genetic studies have implicated the major histocompatability complex (MHC)-human leucocyte antigen (HLA) genes on chromosome 6p21, although this chromosomal region does not explain all of the genetic contribution to the various disorders. Non-MHC-HLA genes, including disease-specific loci, are beginning to be identified and the publication of the draft sequence of the human genome will undoubtedly expediate future discoveries. Combined with the establishment of large cohorts of subjects with disease and the development of technology capable of performing high-throughput genotyping, genetic studies are likely to impact on the future treatment and prevention of the common autoimmune endocrine diseases.  N. Ref:: 107

 

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[113]

TÍTULO / TITLE:  - Liposomal amphotericin B in the treatment of visceral leishmaniasis in immunocompetent patients.

REVISTA / JOURNAL:  - Fundam Clin Pharmacol 2003 Apr;17(2):183-8.

AUTORES / AUTHORS:  - Minodier P; Retornaz K; Horelt A; Garnier JM

INSTITUCIÓN / INSTITUTION:  - Pediatric Emergency Unit, CHU Nord, Chemin des Bourrelly, 13915 Marseille Cedex 20, France. philippe.minodier@ap-hm.fr

RESUMEN / SUMMARY:  - The leishmaniases are protozoan diseases caused by Leishmania parasites. The first-line treatment of its visceral forms is pentavalent antimony (meglumine antimoniate or sodium stibogluconate), but toxicity is frequent with this drug. Moreover antimony unresponsiveness is increasing in Leishmania infantum and L. donovani foci, both in immunocompetent and in immunosuppressed patients. Amphotericin B is a polyene macrolide antibiotic that binds to sterols in cell membranes. It is the most active antileishmanial agent in use. Its infusion-related and renal toxicity may be reduced by lipid-based delivery. Liposomal amphotericin B (AmBisome); Gilead Science, Paris, France) seems to be less toxic than other amphotericin B lipid formulations (Amphocil); Liposome Technology Inc., Menlo Park, CA, USA, Amphotec); Ben Venue Laboratories Inc., Bedford, OH, USA). Optimal drug regimens of AmBisome) vary from one geographical area to another. In the Mediterranean Basin, a total dose of 18 mg/kg (3 mg/kg on days 1-5 and 3 mg/kg on day 10) could be used as first-line treatment of visceral leishmaniasis in immunocompetent patients. In immunocompromised patients, especially those co-infected with HIV, relapses are frequent with AmBisome), as with other drugs.  N. Ref:: 55

 

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[114]

TÍTULO / TITLE:  - Hepatosplenic gamma/delta T-cell lymphoma in immunocompromised patients. Report of two cases and review of literature.

REVISTA / JOURNAL:  - Am J Clin Pathol 2001 Jul;116(1):41-50.

AUTORES / AUTHORS:  - Khan WA; Yu L; Eisenbrey AB; Crisan D; al Saadi A; Davis BH; Hankin RC; Mattson JC

INSTITUCIÓN / INSTITUTION:  - Clinical Pathology Department, William Beaumont Hospital, 3601 W 13 Mile Rd, Royal Oak, MI 48073, USA.

RESUMEN / SUMMARY:  - We describe 2 male patients in whom hepatosplenic gamma/delta T-cell lymphoma (HSTL) developed 6 and 10 years after renal transplantation. The onset was abrupt with systemic symptoms, cytopenia, and hepatosplenomegaly. The histologic examination of the spleen (case 1), liver, and bone marrow revealed sinusoidal infiltrates of markedly abnormal lymphocytes. The neoplastic cells in these cases were CD2+, CD3+, CD4-, CD5-, CD7+, CD8+, CD16+, CD56+, beta F1-negative, and TIA-1-negative. Both cases displayed clonal rearrangement of the T-cell receptor (TCR) delta gene and the TCR beta gene. The spleen in case 1 was positive for Epstein-Barr virus genome and showed TCR-gamma gene rearrangement by polymerase chain reaction. Isochromosome 7 [i(7)(q10)] was found in each case. Both patients died within 4 months of diagnosis. HSTL has been reported in only 5 renal transplant recipients. HSTL may be relatively more frequent in immunocompromised patients compared with the general population.  N. Ref:: 35

 

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[115]

TÍTULO / TITLE:  - Human plasmacytoid-derived dendritic cells and the induction of T-regulatory cells.

REVISTA / JOURNAL:  - Hum Immunol 2002 Dec;63(12):1149-55.

AUTORES / AUTHORS:  - Gilliet M; Liu YJ

INSTITUCIÓN / INSTITUTION:  - DNAX Research Institute, Palo Alto, California, USA. m.gilliet@usz.ch

RESUMEN / SUMMARY:  - Suppression by T-regulatory (Tr) cells is essential for the induction of T-cell tolerance and the prevention of autoimmune diseases, organ rejection, and graft-versus-host disease. Increasing attention has been devoted to understand the role of dendritic cells (DC) in the control of Tr-cell differentiation. Here we review the recent evidence that cluster designation (CD)40-ligand activated plasmacytoid-derived DCs (DC2) have the ability to induce primary Tr-cell differentiation. We propose that in addition to the regulatory functions of immature myeloid DC, Tr-cell induction by DC2 represents a nonredundant mechanism for the safeguard of peripheral T-cell tolerance. DC2 can be used as tool to drive potent antigen specific Tr-cell differentiation and expansion in vitro and in vivo.  N. Ref:: 51

 

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[116]

TÍTULO / TITLE:  - HFE, the MHC and hemochromatosis: paradigm for an extended function for MHC class I.

REVISTA / JOURNAL:  - Tissue Antigens 2003 Apr;61(4):263-75.

AUTORES / AUTHORS:  - Cardoso CS; de Sousa M

INSTITUCIÓN / INSTITUTION:  - Molecular Immunology, Institute for Molecular and Cell Biology, Oporto, Portugal Molecular Pathology and Immunology, Instituto de Ciencias Biomedicas Abel Salazar, OPorto, Portugal.

RESUMEN / SUMMARY:  - HFE was discovered as the hereditary hemochromatosis (HH) gene. It is located on chromosome 6 (6p21.3), 4Mb telomeric to the HLA-A locus, and its product has a structure similar to MHC class I molecules. HFE encodes two frequent mutations: C282Y and H63D. One of these (C282Y) is present in a large proportion of Caucasian HH patients. HFE has a tissue distribution compatible with a role in iron absorption (intestine), recycling (macrophages) and transport to the fetus (placenta).  N. Ref:: 97

 

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[117]

TÍTULO / TITLE:  - Cytokine-based immunointervention in the treatment of autoimmune diseases.

REVISTA / JOURNAL:  - Clin Exp Immunol 2003 May;132(2):185-92.

AUTORES / AUTHORS:  - Adorini L

INSTITUCIÓN / INSTITUTION:  - BioXell, Via Olgettina 58, 20132 Milan, Italy. luciano.adorini@bioxell.com  N. Ref:: 99

 

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[118]

TÍTULO / TITLE:  - Dimerizer-regulated gene expression.

REVISTA / JOURNAL:  - Curr Opin Biotechnol 2002 Oct;13(5):459-67.

AUTORES / AUTHORS:  - Pollock R; Clackson T

INSTITUCIÓN / INSTITUTION:  - ARIAD Gene Therapeutics, 26 Landsdowne Street, Cambridge, MA 02139, USA. roy.pollock@ariad.com

RESUMEN / SUMMARY:  - Control of gene expression using small molecules is a powerful research tool and has clinical utility in the context of regulated gene therapy. Use of chemical inducers of dimerization, or dimerizers, for this purpose has several advantages, including tight regulation, modularity to facilitate iterative improvements, and assembly from human proteins to minimize immune responses in clinical applications. Recent developments include the use of the rapamycin-based dimerizer system to regulate the expression of endogenous genes, the generation of new chemical dimerizers based on FK506, dexamethasone and methotrexate, and progress towards the clinical use of adeno-associated virus and adenovirus vectors regulated by rapamycin analogs.  N. Ref:: 40

 

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[119]

TÍTULO / TITLE:  - DNA methylation and expression of major histocompatibility complex class I and class II transactivator genes in human developmental tumor cells and in T cell malignancies.

REVISTA / JOURNAL:  - Clin Immunol 2003 Oct;109(1):46-52.

AUTORES / AUTHORS:  - van den Elsen PJ; Holling TM; van der Stoep N; Boss JM

INSTITUCIÓN / INSTITUTION:  - Division of Molecular Biology, Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands. pjvdelsen@lumc.nl

RESUMEN / SUMMARY:  - Major histocompatibility complex (MHC) class I and class II molecules play essential roles in the immune response by virtue of their ability to present peptides to T lymphocytes. Given their central role in adaptive immunity, the genes encoding these peptide-presenting molecules are regulated in a tight fashion to meet with local requirements for an adequate immune response. In contrast to MHC class I gene products, which are expressed on almost all nucleated cells, constitutive expression of MHC class II molecules is found in specialized antigen presenting cells of the immune system only. Transcription of both MHC class I and class II genes can be induced by immune regulators and upon cell activation. Transcription of MHC class I genes is mediated by a set of conserved cis acting regulatory elements in their promoters. Of these regulatory elements, MHC class II promoters share the SXY-module. Essential for activation of MHC class II promoters is the class II transactivator (CIITA), which acts through protein/protein interactions with regulatory factors bound to the SXY module. In this review, we discuss the role of DNA methylation in relation to altered expression of MHC class I and CIITA genes as observed in malignancies and in development.  N. Ref:: 68

 

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[120]

TÍTULO / TITLE:  - Alicaforsen. Isis Pharmaceuticals.

REVISTA / JOURNAL:  - Curr Opin Investig Drugs 2001 Oct;2(10):1401-6.

AUTORES / AUTHORS:  - Gewirtz AT; Sitaraman S

INSTITUCIÓN / INSTITUTION:  - Emory University School of Medicine, Department of Pathology and Laboratory Medicine, Atlanta, GA 30322, USA. agewirt@emory.edu

RESUMEN / SUMMARY:  - Alicaforsen (ISIS-2302) is an RNase H-dependent antisense inhibitor of the intercellular adhesion molecule ICAM-1 under development by Isis Pharmaceuticals, for the potential treatment of a variety of inflammatory disorders [175741]. As of April 1997 it was in phase III trials for Crohn’s disease (CD); however, the trial failed and, in December 1999, the company suspended development for this indication [352801]. In October 2000, the company re-initiated development in CD [384820] and new phase III trials had begin by May 2001 [409704]. In August 2000, phase II studies of alicaforsen in an enema formulation for ulcerative colitis and a topical formulation for psoriasis were ongoing [378715]. Development of the compound for the potential treatment of rheumatoid arthritis (RA) was discontinued in 1999 [347579]. By the end of 1998, alicaforsen was in phase II trials for kidney transplant rejection. At this time, these trials were expected to finish in mid-1999 [343460]. However, they were ongoing in September 1999, although no further development has been reported for this indication since that time [338672]. In February 1995, Isis Pharmaceuticals and Boehringer Ingelheim (BI) signed a collaborative agreement on cell adhesion inhibitors, including alicaforsen [174111]. By early 1999, Isis and BI were to decide on the next developmental step for alicaforsen following further analyses of its performance against CD [292915], [315439]. Their joint development agreement was terminated in 1999; Isis regained rights to the product and by September 1999 was in talks to license alicaforsen to another partner for CD [338672]. In June 2000, Cytogenix entered into a sponsored research agreement with Baylor College of Medicine at the Texas Medical Center Houston for the use of its ssDNA expression system for the development of antisense strategies directed against intercellular adhesion molecules for the purpose of reducing lung inflammation and injury in disease states and conditions [369677]. US-05514788, and other patents, cover antisense cell adhesion molecule inhibitors [212289], [234792].  N. Ref:: 45

 

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[121]

TÍTULO / TITLE:  - Accessory proteins that control the assembly of MHC molecules with peptides.

REVISTA / JOURNAL:  - Immunol Res 2001;23(2-3):205-14.

AUTORES / AUTHORS:  - Van Kaer L

INSTITUCIÓN / INSTITUTION:  - Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232-0295, USA. luc.vankaer@mcmail.vanderbilt.edu

RESUMEN / SUMMARY:  - The stable assembly of Major Histocompatibility Complex (MHC) molecules with peptides is controlled by a number of cofactors, including proteins with general housekeeping functions and proteins with dedicated functions in MHC assembly. Recent work in my laboratory has focused on two chaperones, tapasin (tpn) and DM, that play critical roles in the loading of peptides onto MHC class I and MHC class II molecules, respectively. Tapasin is a transmembrane protein that tethers empty class I molecules in the endoplasmic reticulum to the transporter associated with antigen processing. DM is a peptide exchange factor that binds with empty and peptide-loaded class II molecules in endosomal and lysosomal compartments. Although a number of different functions for tapasin and DM have been proposed, emerging evidence suggests that both of these chaperones retain unstable MHC molecules in peptide-loading compartments until they bind with high-affinity peptides. These cofactors therefore promote the surface expression of long-lived MHC-peptide complexes.  N. Ref:: 39

 

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[122]

TÍTULO / TITLE:  - The fission yeast TOR proteins and the rapamycin response: an unexpected tale.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2004;279:85-95.

AUTORES / AUTHORS:  - Weisman R

INSTITUCIÓN / INSTITUTION:  - Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel-Aviv University, 69978 Tel-Aviv, Israel. ronitt@post.tau.ac.il

RESUMEN / SUMMARY:  - The TOR proteins are known as key regulators of cell growth in response to nutritional and mitogenic signals and as targets for the immunosuppressive and anti-cancerous drug rapamycin. The fission yeast Schizosaccharomyces pombe has two TOR homologues, tor1+ and tor2+. Despite their structural similarity, these genes have distinct functions: tor1+ is required under starvation, extreme temperatures, and osmotic or oxidative stress conditions, whereas tor2+ is required under normal growth conditions. Surprisingly, rapamycin does not seem to inhibit the S. pombe TOR-related functions. Rapamycin specifically inhibits sexual development in S. pombe, and this seems to stem from direct inhibition of the S. pombe FKBP12 homologue. Why S. pombe cells are resistant to rapamycin during the growth phase is as yet unclear and awaits further analysis of the TOR-dependent signaling pathways.  N. Ref:: 27

 

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[123]

TÍTULO / TITLE:  - Current treatments for CIDP.

REVISTA / JOURNAL:  - Neurology 2003 Apr 1;60(8 Suppl 3):S16-22.

AUTORES / AUTHORS:  - Ropper AH

INSTITUCIÓN / INSTITUTION:  - Saint Elizabeth’s Medical Center/Tufts University School of Medicine, Boston, MA, USA. Allan_Ropper_MD@cchcs.org

RESUMEN / SUMMARY:  - This article reviews the efficacy and tolerability of currently available therapies, including intravenous immunoglobulin (IVIg), corticosteroids, and plasma exchange (PE), for treatment of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). Data show that current therapies are effective in approximately two-thirds of patients. However, they fail to provide a durable clinical response. Furthermore, current treatments have several limitations that make them problematic for long-term therapy. IVIg dosing is required approximately every 2 to 8 weeks in most patients to maintain improvement. It is expensive, time-consuming to administer, and availability can be a problem. Furthermore, IVIg is a blood product that is associated with rare thromboembolic events. Corticosteroids have poor safety and tolerability profiles, and PE is invasive, time-consuming, expensive, and can be performed only at specialized centers. An alternative to single-agent therapy with current treatments is the use of combination therapy. Combination therapy may increase the duration of response, provide increased efficacy or independent efficacy in unresponsive patients, and reduce the need for standard therapies. Research is needed to find agents suitable for single and combination therapy in CIDP.  N. Ref:: 50

 

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[124]

TÍTULO / TITLE:  - Immunologic and genetic factors in type 1 diabetes.

REVISTA / JOURNAL:  - J Biol Chem. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jbc.org/ 

      ●● Cita: J. of Biological Chemistry: <> 2002 Nov 15;277(46):43545-8. Epub 2002 Sep 20.

      ●● Enlace al texto completo (gratuito o de pago) 1074/jbc.R200012200

AUTORES / AUTHORS:  - Notkins AL

INSTITUCIÓN / INSTITUTION:  - Experimental Medicine Section, Oral Infection and Immunity Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892-4322, USA. anotkins@mail.nih.gov  N. Ref:: 28

 

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[125]

TÍTULO / TITLE:  - Hematopoietic cell transplantation for inherited metabolic diseases: an overview of outcomes and practice guidelines.

REVISTA / JOURNAL:  - Bone Marrow Transplant 2003 Feb;31(4):229-39.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.bmt.1703839

AUTORES / AUTHORS:  - Peters C; Steward CG

INSTITUCIÓN / INSTITUTION:  - Department of Pediatrics, University of Minnesota School of Medicine, Minneapolis, 55455, USA.

RESUMEN / SUMMARY:  - For the past two decades, hematopoietic cell transplantation (HCT) has been used as effective therapy for selected inherited metabolic diseases (IMD) including Hurler (MPS IH) and Maroteaux-Lamy (MPS VI) syndromes, childhood-onset cerebral X-linked adrenoleukodystrophy (X-ALD), globoid-cell leukodystrophy (GLD), metachromatic leukodystrophy (MLD), alpha-mannosidosis, osteopetrosis, and others. Careful pre-HCT evaluation is critical and coordinated, multidisciplinary follow-up is essential in this field of transplantation. The primary goals of HCT for these disorders have been to promote long-term survival with donor-derived engraftment and to optimize the quality of life. Guidelines for HCT and monitoring are provided; a brief overview of long-term results is also presented.  N. Ref:: 131

 

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[126]

TÍTULO / TITLE:  - Composite tissue allotransplantation in chimeric hosts part II. A clinically relevant protocol to induce tolerance in a rat model.

REVISTA / JOURNAL:  - Transplantation 2003 Dec 15;76(11):1548-55.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000085288.12571.65

AUTORES / AUTHORS:  - Prabhune KA; Gorantla VS; Perez-Abadia G; Francois CG; Vossen M; Laurentin-Perez LA; Breidenbach WC; Wang GG; Anderson GL; Pidwell DJ; Barker JH; Maldonado C

INSTITUCIÓN / INSTITUTION:  - Division of Plastic and Reconstructive Surgery, University of Louisville, Louisville, Kentucky, USA.

RESUMEN / SUMMARY:  - BACKGROUND: We and others have shown that mixed allogeneic chimerism induces donor-specific tolerance to composite tissue allografts across major histocompatibility complex barriers without the need for immunosuppression. However, a delay period between bone marrow transplantation and limb allotransplantation is required, making such protocols impractical for clinical application. This study eliminates this delay period in a rat hind limb allotransplantation model by performing mixed allogeneic chimerism induction and transplantation “simultaneously.” METHODS: Group 1 included controls in which naive Wistar Furth (WF) hosts received ACI hind limbs. Group 2 included (ACI-->WF) chimeras that received limbs from third-party donors (Fisher), and group 3 included chimeras that received irradiated (1,050 cGy) ACI limbs. In group 4, WF hosts conditioned with 950 cGy received irradiated (1,050 cGy) ACI limbs followed by infusion of 100 x 10(6) ACI T-cell-depleted bone marrow cells and immunotherapy (tacrolimus and mycophenolate mofetil) for 28 days. Group 5 animals received the same treatment as group 4 animals without immunotherapy. RESULTS: The rats in groups 1 and 2 rejected their limbs within 10 days. Only one rat in group 4 survived to the end of the study. Groups 3 and 5 demonstrated long-term limb survival without rejection or graft-versus-host disease. High levels of donor chimerism (>80%) were achieved and maintained throughout the study. Mixed lymphocyte reaction assays in both groups revealed donor-specific hyporesponsiveness with vigorous third-party reactivity. CONCLUSIONS: This study demonstrated that infusion of donor bone marrow cells into conditioned hosts immediately after limb transplantation results in stable mixed chimerism, robust tolerance, and reliable limb allograft survival.

 

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[127]

TÍTULO / TITLE:  - Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol.

REVISTA / JOURNAL:  - Diabetes. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://diabetes.diabetesjournals.org/ 

      ●● Cita: Diabetes: <> 2001 Apr;50(4):710-9.

AUTORES / AUTHORS:  - Ryan EA; Lakey JR; Rajotte RV; Korbutt GS; Kin T; Imes S; Rabinovitch A; Elliott JF; Bigam D; Kneteman NM; Warnock GL; Larsen I; Shapiro AM

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, Surgical Medical Research Institute, University of Alberta, Edmonton, Canada. edmond.ryan@ualberta.ca

RESUMEN / SUMMARY:  - Islet transplantation offers the prospect of good glycemic control without major surgical risks. After our initial report of successful islet transplantation, we now provide further data on 12 type 1 diabetic patients with brittle diabetes or problems with hypoglycemia previous to 1 November 2000. Details of metabolic control, acute complications associated with islet transplantation, and long-term complications related to immunosuppression therapy and diabetes were noted. Insulin secretion, both acute and over 30 min, was determined after intravenous glucose tolerance tests (IVGTTs). The median follow-up was 10.2 months (CI 6.5-17.4), and the longest was 20 months. Glucose control was stable, with pretransplant fasting and meal tolerance-stimulated glucose levels of 12.5+/-1.9 and 20.0+/-2.7 mmol/l, respectively, but decreased significantly, with posttransplant levels of 6.3+/-0.3 and 7.5+/-0.6 mmol/l, respectively (P < 0.006). All patients have sustained insulin production, as evidenced by the most current baseline C-peptide levels 0.66+/-0.06 nmol/l, increasing to 1.29+/-0.25 nmol/l 90 min after the meal-tolerance test. The mean HbA1c level decreased from 8.3+/-0.5% to the current level of 5.8+/-0.1% (P < 0.001). Presently, four patients have normal glucose tolerance, five have impaired glucose tolerance, and three have post-islet transplant diabetes (two of whom need oral hypoglycemic agents and low-dose insulin (<10 U/day). Three patients had a temporary increase in their liver-function tests. One patient had a thrombosis of a peripheral branch of the right portal vein, and two of the early patients had bleeding from the hepatic needle puncture site; but these technical problems were resolved. Two patients had transient vitreous hemorrhages. The two patients with elevated creatinine levels pretransplant had a significant increase in serum creatinine in the long term, although the mean serum creatinine of the group was unchanged. The cholesterol increased in five patients, and lipid-lowering therapy was required for three patients. No patient has developed cytomegalovirus infection or disease, posttransplant lymphoproliferative disorder, malignancies, or serious infection to date. None of the patients have been sensitized to donor antigen. In 11 of the 12 patients, insulin independence was achieved after 9,000 islet equivalents (IEs) per kilogram were transplanted. The acute insulin response and the insulin area under the curve (AUC) after IVGTT were consistently maintained over time. The insulin AUC from the IVGTT correlated to the number of islets transplanted, but more closely correlated when the cold ischemia time was taken into consideration (r = 0.83, P < 0.001). Islet transplantation has successfully corrected labile type 1 diabetes and problems with hypoglycemia, and our results show persistent insulin secretion. After a minimum of 9,000 IEs per kilogram are provided, insulin independence is usually attained. An elevation of creatinine appears to be a contraindication to this immunosuppressive regimen. For the subjects who had labile type 1 diabetes that was difficult to control, the risk-to-benefit ratio is in favor of islet transplantation.

 

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[128]

- Castellano -

TÍTULO / TITLE:Terapia individualizada con azatioprina o 6-mercaptopurina mediante monitorizacion de la actividad de la tiopurina metiltransferasa (TPMT). Individualized therapy with azathioprine or 6-mercaptopurine by monitoring thiopurine methyl-transferase (TPMT) activity.

REVISTA / JOURNAL:  - Rev Clin Esp. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://db.doyma.es/ 

      ●● Cita: Revista Clínica Española: <> 2002 Oct;202(10):555-62.

AUTORES / AUTHORS:  - Gisbert JP; Gomollon F; Mate J; Pajares JM

INSTITUCIÓN / INSTITUTION:  - Servicio de Aparato Digestivo. Hospital Universitario de La Princesa. Madrid. España. gisbert@meditex.es  N. Ref:: 83

 

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[129]

TÍTULO / TITLE:  - Metabolic and autocrine regulation of the mammalian target of rapamycin by pancreatic beta-cells.

REVISTA / JOURNAL:  - Diabetes. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://diabetes.diabetesjournals.org/ 

      ●● Cita: Diabetes: <> 2002 Oct;51(10):2877-85.

AUTORES / AUTHORS:  - McDaniel ML; Marshall CA; Pappan KL; Kwon G

INSTITUCIÓN / INSTITUTION:  - Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. mcdaniel@pathology.wustl.edu

RESUMEN / SUMMARY:  - Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. mTOR-mediated phosphorylation of both the translational repressor eukaryotic initiation factor 4E binding protein-1 and p70 S6 kinase are early events that control the translation initiation process. Rapamycin, an inhibitor of mTOR, is a potent immunosuppressant due, in part, to its ability to interfere with T-cell activation at the level of translation, and it has gained a prominent role in preventing the development and progression of rejection in pancreatic islet transplant recipients. The characterization of the insulin signaling cascade that modulates mTOR in insulin-sensitive tissues has been a major focus of investigation. Recently, the ability of nutrients, in particular the branched-chain amino acid leucine, to activate mTOR independent of insulin by a process designated as nutrient signaling has been identified. The beta-cell expresses components of the insulin signaling cascade and utilizes the metabolism of nutrients to affect insulin secretion. These combined transduction processes make the beta-cell an unique cell to study metabolic and autocrine regulation of mTOR signaling. Our studies have described the ability of insulin and IGFs in concert with the nutrients leucine, glutamine, and glucose to modulate protein translation through mTOR in beta-cells. These findings suggest that mitochondria-derived factors, ATP in particular, may be responsible for nutrient signaling. The significance of these findings is that the optimization of mitochondrial function is not only important for insulin secretion but may significantly impact the growth and proliferation of beta-cells through these mTOR signaling pathways.  N. Ref:: 51

 

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[130]

TÍTULO / TITLE:  - The Tubingen approach: identification, selection, and validation of tumor-associated HLA peptides for cancer therapy.

REVISTA / JOURNAL:  - Cancer Immunol Immunother 2004 Mar;53(3):187-95. Epub 2004 Jan 31.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00262-003-0480-x

AUTORES / AUTHORS:  - Singh-Jasuja H; Emmerich NP; Rammensee HG

INSTITUCIÓN / INSTITUTION:  - Department of Immunology, Institute for Cell Biology, University of Tubingen, Auf der Morgenstelle 15, 72076, Tubingen, Germany.

RESUMEN / SUMMARY:  - There is substantial need for molecularly defined tumor antigens to prime cytotoxic T cells in vivo for cancer immunotherapy, especially in the case of tumor entities for which only a few tumor antigens have been defined so far. In this review, we present the “Tubingen approach” to identify, select, and validate large numbers of MHC/HLA class I-associated peptides derived from tumor-associated antigens. Step 1 is the identification of naturally presented HLA-associated peptides directly from primary tumor cells. Step 2 is selection of tumor-associated peptides from step 1 by differential gene expression analysis and data mining. Step 3 is validation of selected candidates by monitoring in vivo T-cell responses in the context of patient-individualized immunizations. Our approach combines methods from genomics, proteomics, bioinformatics, and T-cell immunology. The aim is to develop effective immunotherapeutics consisting of multiple tumor-associated epitopes in order to induce a broad and specific immune response against cancer cells.  N. Ref:: 67

 

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[131]

TÍTULO / TITLE:  - Peptide register shifting within the MHC groove: theory becomes reality.

REVISTA / JOURNAL:  - Mol Immunol 2004 Feb;40(14-15):1033-9.

      ●● Enlace al texto completo (gratuito o de pago) 1016/j.molimm.2003.11.016

AUTORES / AUTHORS:  - Bankovich AJ; Girvin AT; Moesta AK; Garcia KC

INSTITUCIÓN / INSTITUTION:  - Department of Microbiology & Immunology, Stanford University School of Medicine, Fairchild D319, Stanford, CA 94305-5124, USA.

RESUMEN / SUMMARY:  - Degeneracy in immune recognition is usually thought of in terms of the astonishing ability of the T cell receptor to recognize an enormously diverse array of peptides bound to major histocompatibility complex (MHC) molecules. However, in this essay we discuss an alternative aspect of degeneracy in T cell recognition: the notion that peptides can assume different “registers” in the groove of a single MHC molecule, as first suggested and demonstrated by Sercarz and co-workers (reviewed in [J. autoimmun. 16 (2001) 201]). There is now abundant evidence, derived from functional, biochemical and structural studies, that single peptides can assume alternative, unpredictable binding registers by frameshifting within the MHC groove [Nat. Immunol. 3 (2002) 175;; J. Exp. Med. 187 (1998) 1505; J. Mol. Biol. 304 (2000) 177; Biochemistry 38 (1999) 16663; J. Exp. Med. 197 (2003) 1391; Eur. J. Immunol. 19 (1989) 681]. Hence, register shifting adds an additional dimension to the concept of degeneracy. In fact, the possibility of register shifting multiplies the universe of peptide-MHC (pMHC) surfaces that a TCR must recognize by an unknown, perhaps enormous factor. Register shifting also has profound implication for autoimmunity: (1) as a mechanism to “mask” autoantigenic epitopes during thymic education [Immunol. Rev. 169 (1999) 147; Immunity 17 (2002) 83]; and (2) as a possible source for pMHC complexes capable of molecular mimicry.  N. Ref:: 45

 

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[132]

TÍTULO / TITLE:  - mTOR as a positive regulator of tumor cell responses to hypoxia.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2004;279:299-319.

AUTORES / AUTHORS:  - Abraham RT

INSTITUCIÓN / INSTITUTION:  - Program in Signal Transduction Research, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA. abraham@burnham.org

RESUMEN / SUMMARY:  - Rapamycin is a clinically approved immunosuppressive agent that has recently shown promising antitumor activities in human patients. In contrast to many conventional chemotherapeutic agents, rapamycin displays a remarkably high level of selectivity for certain types of tumors. The pharmacological activities of rapamycin are attributable to the functional inhibition of a single target protein, termed the mammalian target of rapamycin (mTOR). Because mTOR is widely expressed in both normal and transformed cells, variations in mTOR expression levels are likely not a primary determinant of tumor sensitivity to rapamycin. However, recent studies highlighted an intriguing link between cancer cell sensitivity to rapamycin and deregulated signaling through the phosphoinositide (PI) 3-kinase pathway. These findings have prompted a search for cancer-related responses that are jointly regulated by the PI 3-kinase signaling cascade and mTOR. The oxygen-regulated transcription factor, hypoxia-induced factor (HIF)-1, has emerged as a candidate target for both of these two highly interactive signaling proteins. Here we review evidence that mTOR functions as a positive regulator of HIF-1-dependent responses to hypoxic stress in human cancer cells.  N. Ref:: 71

 

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[133]

TÍTULO / TITLE:  - TGF-beta expression in protocol transplant liver biopsies: a comparative study between cyclosporine-A (CyA) and tacrolimus (FK 506) immunosuppression.

REVISTA / JOURNAL:  - Transplant Proc 2001 Feb-Mar;33(1-2):1378-80.

AUTORES / AUTHORS:  - Mohamed MA; Burt AD; Robertson H; Kirby JA; Talbot D

INSTITUCIÓN / INSTITUTION:  - Transplant Immunobiology Group, Department of Surgery, University of Newcastle, NE2 4HH, Newcastle Upon Tyne, UK.

 

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[134]

TÍTULO / TITLE:  - HLA class II tetramers: tools for direct analysis of antigen-specific CD4+ T cells.

REVISTA / JOURNAL:  - Arthritis Rheum 2002 Jan;46(1):5-12.

AUTORES / AUTHORS:  - Nepom GT; Buckner JH; Novak EJ; Reichstetter S; Reijonen H; Gebe J; Wang R; Swanson E; Kwok WW

INSTITUCIÓN / INSTITUTION:  - Virginia Mason Research Center and University of Washington School of Medicine, Seattle 98101-2795, USA. nepom@vmresearch.org

RESUMEN / SUMMARY:  - Immunotherapies for human autoimmune and immune-mediated diseases are proliferating rapidly, and with these changes comes the opportunity to monitor patients for immune responses to therapy based on early surrogate markers for clinical responses. Class II tetramers have the potential to serve as these sorts of markers for immune monitoring, and thereby assist with patient management, therapy selection, and improved outcomes. However, important issues of TCR avidity require resolution, because much is still unknown regarding location, quantitation, and characterization of the human T cell response. Opportunities for application of tetramer technologies in the near future will enable both clinical progress and the development of new insights into human CD4+ T cell biology in vivo.  N. Ref:: 39

 

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[135]

TÍTULO / TITLE:  - Alternative concepts of suicide gene therapy for graft-versus-host disease after adoptive immunotherapy.

REVISTA / JOURNAL:  - Acta Haematol 2003;110(2-3):132-8.

      ●● Enlace al texto completo (gratuito o de pago) 1159/000072462

AUTORES / AUTHORS:  - Kramm CM

INSTITUCIÓN / INSTITUTION:  - Department of Pediatric Hematology, Oncology, and Immunology, University Hospital Dusseldorf, Dusseldorf, Germany. kramm@uni-duesseldorf.de

RESUMEN / SUMMARY:  - T-cell suicide gene therapy represents a promising novel treatment strategy for graft-versus-host disease following adoptive immunotherapy after allogeneic hematopoietic stem cell transplantation. The clinical efficiency of this approach is still hampered by several obstacles including induction of alloresponses due to the use of immunogenic suicide and selection genes, genetic inactivation of suicide genes, and functional immunological impairment after retroviral transduction with extensive in vitro stimulation. New concepts as possible solutions to these limitations are discussed.  N. Ref:: 36

 

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[136]

TÍTULO / TITLE:  - Not such a dismal science: the economics of protein synthesis, folding, degradation and antigen processing.

REVISTA / JOURNAL:  - Trends Cell Biol 2001 Jul;11(7):294-7.

AUTORES / AUTHORS:  - Yewdell JW

INSTITUCIÓN / INSTITUTION:  - Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Disease, 20892-0440, Bethesda, MD, USA. jyewdell@nih.gov

RESUMEN / SUMMARY:  - There is a pronounced tendency among cell biologists to focus on qualitative aspects of cell physiology. The remarkable accomplishments of evolution in creating cells can only be fully appreciated, however, by combining this qualitative analysis with a quantitative assessment of cellular constituents and processes. Here, I consider the overall protein economy of cells as it relates to recent advances in understanding protein folding, ubiquitin-targeted proteasome-mediated degradation of proteins and the generation of peptide ligands for major histocompatibility complex (MHC) class I molecules.  N. Ref:: 29

 

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[137]

TÍTULO / TITLE:  - MHC class II deficiency: a disease of gene regulation.

REVISTA / JOURNAL:  - Medicine (Baltimore) 2001 Nov;80(6):405-18.

AUTORES / AUTHORS:  - Villard J; Masternak K; Lisowska-Grospierre B; Fischer A; Reith W

INSTITUCIÓN / INSTITUTION:  - Immunology and Transplant Unit, Division of Immunology and Allergology, Geneva University Hospital, Geneva, Switzerland. Jean.Villard@hcuge.ch  N. Ref:: 115

 

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[138]

TÍTULO / TITLE:  - Dendritic cells and second signal blockade: a step toward allograft tolerance?

REVISTA / JOURNAL:  - Transplantation 2002 Jan 15;73(1 Suppl):S1-2.

AUTORES / AUTHORS:  - Rifle G; Mousson C

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology-Intensive Care-Transplantation and UPRES EA563, Faculty of Medicine, University of Bourgogne, Dijon, France. gerard.rifle@chu-dijon.fr

RESUMEN / SUMMARY:  - The ultimate goal of clinical organ transplantation is to induce immunological tolerance to the allograft. Dendritic cells, the main antigen-presenting cells, are a complex system including numerous subsets, capable of inducing T-cell activation, and thus initiating an immune response, but also of inducing tolerance. In organ transplantation, the problem is even more complex because of the coexistence of dendritic cells from the donor, responsible for direct recognition of foreign antigens by T cells, and dendritic cells from the recipient, responsible for indirect antigen presentation to host T cells. Among the various methods of immunomanipulation aiming at inducing tolerance, blockade of the second signal pathway by monoclonal antibodies (anti-CD28, anti-B7, anti CD40, anti-CD40L, CTLA4-Ig) has yielded promising but incomplete results. Viral transfection of recipient immature dendritic cells encoding for immunosuppressive or apoptotic molecules, such as interleukin 10, transforming growth factor-beta, CTLA4-Ig, or Fas ligand, is also able to induce hyporesponsiveness. Another very promising method consists of associating donor cells (bone marrow cells, CD34+ cells, dendritic cells) and polyclonal or monoclonal antibodies (anti-CD4, anti-CD40L, CTLA4-Ig) to induce microchimerism and partial tolerance. Reflecting on these data would seem to be an interesting direction for future prospects.  N. Ref:: 24

 

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[139]

TÍTULO / TITLE:  - At the crossroads of cell biology and immunology: DRiPs and other sources of peptide ligands for MHC class I molecules.

REVISTA / JOURNAL:  - J Cell Sci. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://jcs.biologists.org/ 

      ●● Cita: J. of Cell Science: <> 2001 Mar;114(Pt 5):845-51.

AUTORES / AUTHORS:  - Yewdell JW; Schubert U; Bennink JR

INSTITUCIÓN / INSTITUTION:  - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-0440, USA.

RESUMEN / SUMMARY:  - CD8(+) T cells are a critical element of vertebrate immune responses to viruses and other intracellular parasites. They roam the body, monitoring cells for the presence of foreign peptides associated with MHC class I molecules of the major histocompatibility complex (MHC). Although it is clear that most of these peptides are generated through the action of proteasomes, the nature of the substrates degraded by proteasomes is an open question. Recent findings indicate that the major pool of substrates consists of a heterogeneous subset of proteins that are degraded within minutes of their synthesis. Evidence suggests that the fraction of newly synthesized proteins targeted for destruction is remarkably high - 30% or more, depending on cell type - possibly because they are defective in some way and cannot reach their intended conformation or location cellular in a time frame deemed appropriate by cells.  N. Ref:: 47

 

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[140]

TÍTULO / TITLE:  - CD30+ T-cell lymphoma in a patient with psoriasis treated with ciclosporin and infliximab.

REVISTA / JOURNAL:  - Br J Dermatol 2003 Jul;149(1):170-3.

AUTORES / AUTHORS:  - Mahe E; Descamps V; Grossin M; Fraitag S; Crickx B

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Bichat-Claude Bernard Hospital, 46 Rue Henri-Huchard, Paris Cedex 18, France. emmanuel.mahe@bch.ap-hop-paris.fr

RESUMEN / SUMMARY:  - There is a known relationship between the use of immunosuppressive therapies and the development of lymphoproliferative malignancies. These lymphomas are mainly B-cell nonHodgkin’s lymphomas associated with Epstein-Barr virus. Most cases concern classical immunosuppressive treatments including ciclosporin and methotrexate. A relationship between the new antitumour necrosis factor (TNF)-alpha agents and lymphoproliferative malignancies is debated. Patients with psoriasis on immunosuppressive therapies, mainly ciclosporin, are considered to have a low risk of developing lymphoid proliferation. We report a patient with erythrodermic psoriasis treated with ciclosporin and infliximab who developed a CD30+ T-cell lymphoma. This lymphoma regressed after stopping these treatments. In this case, the anti-TNF-alpha agent may have played a role in association with ciclosporin in the development of the lymphoproliferative disorder. Whereas the combination of anti-TNF-alpha therapies with methotrexate has been well studied, their combination with ciclosporin has been evaluated only in a few patients. Psoriatic patients who may require anti-TNF-alpha treatment have often been or will be treated with ciclosporin. The combination of ciclosporin and anti-TNF-alpha warrants further investigation.  N. Ref:: 17

 

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[141]

TÍTULO / TITLE:  - Efficacy of pneumococcal polysaccharide vaccine in immunocompetent adults: a meta-analysis of randomized trials.

REVISTA / JOURNAL:  - Vaccine 2001 Sep 14;19(32):4780-90.

AUTORES / AUTHORS:  - Cornu C; Yzebe D; Leophonte P; Gaillat J; Boissel JP; Cucherat M

INSTITUCIÓN / INSTITUTION:  - Service of Clinical Pharmacology, EA643, Lyon University Hospital, Faculte de Medicine RTH Laennec, BP 8071, 69376, Cedex 08, Lyon, France. catherine.cornu@upcl.univ-lyon1.fr

RESUMEN / SUMMARY:  - The use of pneumococcal polysaccharide vaccine (PPV) is low in some countries, maybe because of doubts regarding its efficacy. This meta-analysis aims at combining evidence from randomized trials of PPV assessing its efficacy in preventing Streptococcus pneumoniae related diseases in immunocompetent adults. In the fourteen trials totalling 48,837 patients retrieved, PPV prevents definite pneumococcal pneumonia by 71%, presumptive pneumococcal pneumonia by 40%, and mortality due to pneumonia by 32%, but not all-cause pneumonia or death. No preventive effect was seen in the subgroup of patients aged 55 years or more, possibly due to a lack of statistical power.

 

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[142]

TÍTULO / TITLE:  - The value of pulse cyclophosphamide in ANCA-associated vasculitis: meta-analysis and critical review.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2001 Oct;16(10):2018-27.

AUTORES / AUTHORS:  - de Groot K; Adu D; Savage CO

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Medical School Hannover, Germany. kirsten@de-grot.de

RESUMEN / SUMMARY:  - BACKGROUND: The study aimed at studying efficacy and adverse effects of pulse cyclophosphamide (pCyc) treatment and to compare it to continuous cyclophosphamide (cCyc) for induction of remission in ANCA-associated vasculitides from data in the published literature. METHODS: A Medline search identified 14 studies, containing more than five patients. From the 11 non-randomized studies, data on outcome following pCyc treatment were extracted. Results were given as fraction of the number of evaluable patients. A meta-analysis was performed on the three prospective, randomized controlled trials to compare outcomes concerning remission, relapses, infection, leucopenia, death and renal failure in patients treated with pCyc as opposed to cCyc. RESULTS: The 11 non-randomized studies comprised 202 patients receiving pCyc. Cyc pulses of 375-1000 mg/sqm/pulse were applied at weekly to monthly intervals with different concomitant prednisolone regimens and variable adjunctive therapy. Complete remission was achieved in 112/191, partial remission in 23/191 evaluable patients. Relapses occurred in 68/135 patients, 40/115 patients were non-responders. Leucopenia, infections, haemorrhagic cystitis, and deaths were rare. The meta-analysis, comprising 143 patients, showed that pCyc compared with cCyc treatment was significantly less likely to fail to induce remission (OR 0.29; 95% CI 0.12-0.73) and had a significantly lower risk of infection (OR 0.45; 95% CI 0.23-0.89) and leucopenia (OR 0.36; 95% CI 0.17-0.78). Relapses occurred slightly, although not statistically significantly, more often under pCyc treatment (OR 1.79; 95% CI 0.85-3.75). There were no differences in end-stage renal failure or deaths between the two regimens. CONCLUSIONS: The currently available, rather sparse data show that pCyc is less toxic than cCyc therapy and is an at least equally potent inductor of remission, but possibly at the expense of a higher relapse rate. The existing data do not give sufficient information on outcomes as time to remission and relapse, irreversible damage or quality of life without which a treatment regimen cannot satisfactorily be evaluated today. A large prospective randomized controlled trial is needed to address these issues and their relative importance.

 

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[143]

TÍTULO / TITLE:  - Immune tolerance induction in patients with haemophilia A with inhibitors: a systematic review.

REVISTA / JOURNAL:  - Haemophilia 2003 Jul;9(4):436-63.

AUTORES / AUTHORS:  - Wight J; Paisley S; Knight C

INSTITUCIÓN / INSTITUTION:  - ScHARR, University of Sheffield, Sheffield, S1 4DA, UK. j.p.wight@sheffield.ac.uk

RESUMEN / SUMMARY:  - In some patients with haemophilia A, therapeutically administered factor VIII (FVIII) comes to stimulate the production of antibodies (inhibitors) which react with FVIII to render it ineffective. As a result, FVIII cannot be used prophylactically and patients become liable to recurrent bleeds. There are two elements to the management of patients with inhibitors: the treatment of bleeding episodes, and attempts to abolish inhibitor production through the induction of immune tolerance. This paper reports a systematic review of the best available evidence of clinical effectiveness in relation to immune tolerance induction (ITI) in patients with haemophilia A with inhibitors. Owing to the lack of randomized controlled trials on this topic, broad inclusion criteria with regard to study design were applied in order to assess the best available evidence for each intervention. As a result of the clinical and methodological heterogeneity of the evidence, it was not appropriate to pool data across studies; instead, data were synthesized using tabulation and qualitative narrative assessment. The International Registry provides the most reliable estimate of the proportion of successful cases of ITI [48.7%, 95% confidence interval (CI) 42.6-52.7%]. The duration of effect is unclear, but relapses appear to be infrequent. The International Registry shows a rate of relapse of 15% at 15 years. The comparative effectiveness of different protocols is uncertain, as no trials have been undertaken which compare them directly. However, the evidence suggests that the Bonn protocol may be more effective than the Malmo or low-dose protocols. There is no good evidence that immunosuppressive drug regimens are effective.  N. Ref:: 54

 

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[144]

TÍTULO / TITLE:  - Elucidating TOR signaling and rapamycin action: lessons from Saccharomyces cerevisiae.

REVISTA / JOURNAL:  - Microbiol Mol Biol Rev. - Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://mmbr.asm.org/ 

      ●● Cita: Microbiology & Molecular Biology Reviews: <> 2002 Dec;66(4):579-91, table of contents.

AUTORES / AUTHORS:  - Crespo JL; Hall MN

INSTITUCIÓN / INSTITUTION:  - Division of Biochemistry, Biozentrum, University of Basel, CH-4056 Basel, Switzerland.

RESUMEN / SUMMARY:  - TOR (target of rapamycin) is a phosphatidylinositol kinase-related protein kinase that controls cell growth in response to nutrients. Rapamycin is an immunosuppressive and anticancer drug that acts by inhibiting TOR. The modes of action of TOR and rapamycin are remarkably conserved from S. cerevisiae to humans. The current understanding of TOR and rapamycin is derived largely from studies with S. cerevisiae. In this review, we discuss the contributions made by S. cerevisiae to understanding rapamycin action and TOR function.  N. Ref:: 171

 

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[145]

TÍTULO / TITLE:  - Structures of calcineurin and its complexes with immunophilins-immunosuppressants.

REVISTA / JOURNAL:  - Biochem Biophys Res Commun 2003 Nov 28;311(4):1095-102.

AUTORES / AUTHORS:  - Ke H; Huai Q

INSTITUCIÓN / INSTITUTION:  - Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, The University of North Carolina, Chapel Hill, NC 27599-7260, USA. hke@med.unc.edu

RESUMEN / SUMMARY:  - Calcineurin (CN) is a Ca(2+)/calmodulin-dependent serine/threonine protein phosphatase and is involved in many physiological processes such as T-cell activation and cardiac hypertrophy. The crystal structures of CN and its complexes with FKBP12-FK506 and cyclophilin-cyclosporin showed that the two structurally unrelated immunophilins-immunosuppressants bind to a common composite surface made up of the residues from both catalytic subunit and regulatory subunit of CN. The recognition of the immunophilins and immunosuppressive drugs is achieved by common but few distinct CN residues. However, the binding pattern of FKBP12-FK506 such as hydrogen bonding is significantly different from that of CyPA-CsA. This common but distinct recognition may indicate capacity of the composition surface for binding of other inhibitory proteins. The recognition site and the active site are adjacent and form an “L” shaped cleft. This implies that the immunophilin recognition site may also serve as a recognition site to define the narrow substrate specificity of calcineurin.  N. Ref:: 61

 

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[146]

TÍTULO / TITLE:  - Tryptophan catabolism and regulation of adaptive immunity.

REVISTA / JOURNAL:  - J Immunol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jimmunol.org/ 

      ●● Cita: J. of Immunology: <> 2003 Jun 15;170(12):5809-13.

AUTORES / AUTHORS:  - Mellor AL; Munn DH

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, Medical College of Georgia, Augusta GA 30912, USA.  N. Ref:: 40

 

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[147]

TÍTULO / TITLE:  - Interventions for mucous membrane pemphigoid/cicatricial pemphigoid and epidermolysis bullosa acquisita: a systematic literature review.

REVISTA / JOURNAL:  - Arch Dermatol 2002 Mar;138(3):380-4.

AUTORES / AUTHORS:  - Kirtschig G; Murrell D; Wojnarowska F; Khumalo N

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Oxford Radcliffe Hospital, England. G.Kirtschig@vumc.nl

RESUMEN / SUMMARY:  - OBJECTIVE: To identify and critically evaluate evidence from randomized controlled trials for the efficacy of treatments for mucous membrane pemphigoid (MMP)/cicatricial pemphigoid (CP) and epidermolysis bullosa acquisita (EBA). SEARCH STRATEGY: Review of MEDLINE from 1966 through March 2000, EMBASE from 1980 through March 2000, and the Cochrane Controlled Trials Register (February 28, 2001) to identify randomized controlled trials for the efficacy of treatments in MMP/CP and EBA. SELECTION CRITERIA: All randomized controlled trials of therapeutic interventions that included patients with MMP/CP or EBA confirmed by immunofluorescence study findings. All age groups were included. RESULTS: We found 2 small randomized controlled trials of MMP/CP, both conducted in patients with severe eye involvement. We were not able to identify a randomized controlled trial of therapeutic interventions in EBA. CONCLUSIONS: There is evidence from 2 small trials that severe ocular CP responds best to treatment with cyclophosphamide, and mild to moderate disease seems effectively suppressed by treatment with dapsone. No treatment recommendations can be made for EBA because to our knowledge no randomized controlled trials are published. Even though systemic corticosteroids are regarded as the gold standard in the treatment of MMP/CP and EBA, there is poor evidence from the literature that they are the best treatment for these diseases.  N. Ref:: 50

 

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[148]

TÍTULO / TITLE:  - Multidrug resistance reversal agents.

REVISTA / JOURNAL:  - J Med Chem 2003 Nov 6;46(23):4805-17.

      ●● Enlace al texto completo (gratuito o de pago) 1021/jm030183a

AUTORES / AUTHORS:  - Robert J; Jarry C

INSTITUCIÓN / INSTITUTION:  - Institut Bergonie, 229, Cours de l’Argonne, 33076 Bordeaux Cedex, France. robert@bergonie.org  N. Ref:: 151

 

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[149]

TÍTULO / TITLE:  - Structure and function of major histocompatibility complex (MHC) class I specific receptors expressed on human natural killer (NK) cells.

REVISTA / JOURNAL:  - Mol Immunol 2002 Feb;38(9):637-60.

AUTORES / AUTHORS:  - Borrego F; Kabat J; Kim DK; Lieto L; Maasho K; Pena J; Solana R; Coligan JE

INSTITUCIÓN / INSTITUTION:  - Receptor Cell Biology Section, Laboratory of Allergic Diseases, NIAID, NIH, Twinbrook II, Room 205, 12441 Parklawn Dr., Rockville, MD 20852, USA.

RESUMEN / SUMMARY:  - Natural killer (NK) cells express receptors that are specific for MHC class I molecules. These receptors play a crucial role in regulating the lytic and cytokine expression capabilities of NK cells. In humans, three distinct families of genes have been defined that encode for receptors of HLA class I molecules. The first family identified consists of type I transmembrane molecules belonging to the immunoglobulin (Ig) superfamily and are called killer cell Ig-like receptors (KIR). A second group of receptors belonging to the Ig superfamily, named ILT (for immunoglobulin like transcripts), has more recently been described. ILTs are expressed mainly on B, T and myeloid cells, but some members of this group are also expressed on NK cells. They are also referred to as LIRs (for leukocyte Ig-like receptor) and MIRs (for macrophage Ig-like receptor). The ligands for the KIR and some of the ILT receptors include classical (class Ia) HLA class I molecules, as well as the nonclassical (class Ib) HLA-G molecule. The third family of HLA class I receptors are C-type lectin family members and are composed of heterodimers of CD94 covalently associated with a member of the NKG2 family of molecules. The ligand for most members is the nonclassical class I molecule HLA-E. NKG2D, a member of the NKG2 family, is expressed as a homodimer, along with the adaptor molecule DAP10. The ligands of NKG2D include the human class I like molecules MICA and MICB, and the recently described ULBPs. Each of these three families of receptors has individual members that can recognize identical or similar ligands yet signal for activation or inhibition of cellular functions. This dichotomy correlates with particular structural features present in the transmembrane and intracytoplasmic portions of these molecules.In this review we will discuss the molecular structure, specificity, cellular expression patterns, and function of these HLA class I receptors, as well as the chromosomal location and genetic organization.  N. Ref:: 224

 

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[150]

TÍTULO / TITLE:  - Prevention by dietary (n-6) polyunsaturated phosphatidylcholines of intrahepatic cholestasis induced by cyclosporine A in animals.

REVISTA / JOURNAL:  - Life Sci 2003 Jun 13;73(4):381-92.

AUTORES / AUTHORS:  - Chanussot F; Benkoel L

INSTITUCIÓN / INSTITUTION:  - INSERM U. 476, Faculte de Medecine, 27 bd Jean Moulin, 13385 Marseille cedex 05, France. Francoise.Chanussot@medecine.univ-mrs.fr

RESUMEN / SUMMARY:  - Previous findings showed that dietary (n-6) polyunsaturated phosphatidylcholines (vegetable lecithin) could efficiently prevent intrahepatic cholestasis induced by cyclosporine A in rats. Mechanistic studies showed that expressions in rat liver of Na(+), K(+)-ATPase, Ca(2+), Mg(2+)-ATPase and F-actin were both decreased by drug administration and both enhanced by (n-6) lecithin enriched diet. There is a possible direct effect of phosphatidylcholines, vectors of polyunsaturated fatty acids provided by the metabolism of the dietary lecithin, on the aforesaid hepatic parameters. Such modulations by drug and diet result in reversed modifications of membrane composition and fluidity. Final outcome is decreased and enhanced bile lipid secretion by cyclosporine and vegetable lecithin enriched diet respectively. Moreover, we advance the hypothesis of a bypass process including a separate and functional actin-independent way for the non micellar and phospholipid-dependent secretion of bile lipids. The relationships between the ATPases, the microfilament components such as F-actin and the different transporters still remain to be clarified. Furthermore, one can speculate on beneficial effects in humans of diets enriched in vegetable lecithins that might prevent cholestasis induced by cyclosporine A.  N. Ref:: 75

 

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[151]

TÍTULO / TITLE:  - Oxidized LDL autoantibodies, endothelial dysfunction, and transplant-associated arteriosclerosis.

REVISTA / JOURNAL:  - Arterioscler Thromb Vasc Biol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://atvb.ahajournals.org/ 

      ●● Cita: Arteriosclerosis & Thrombosis :  A J. Vasc Biol: <> 2002 Dec 1;22(12):1950-1.

AUTORES / AUTHORS:  - Alexander RW  N. Ref:: 19

 

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[152]

TÍTULO / TITLE:  - Alloantibody and xenoantibody cross-reactivity in transplantation.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 15;77(1):1-5.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000105116.74032.63

AUTORES / AUTHORS:  - Cooper DK; Tseng YL; Saidman SL

INSTITUCIÓN / INSTITUTION:  - Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA. David.Cooper@tbrc.mgh.harvard.edu

RESUMEN / SUMMARY:  - The recent availability of pigs homozygous for alpha1,3-galactosyltransferase gene knockout, and improved immunosuppressive regimens that prevent an elicited antibody response, are expected to contribute to significantly increased survival of pig organs transplanted into primates, bringing clinical trials of xenotransplantation closer. Patients highly sensitized to human leukocyte antigens, who may be precluded from obtaining a human donor organ, would be one group that might benefit from xenotransplantation. However, there have been few studies on whether there is cross-reactivity of anti-human leukocyte antigen antibodies with pig antigens. What data there are suggest that such cross-reactivity exists and that this may be detrimental to the outcome after transplantation of a pig organ. Neither is it known whether sensitization after a pig xenograft would preclude subsequent allotransplantation, although the data available suggest that this will not be the case. Further investigation on allo- and xenoantibody cross-reactivity is required.  N. Ref:: 44

 

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[153]

TÍTULO / TITLE:  - Shared epitopes and rheumatoid arthritis: disease associations in Greece and meta-analysis of Mediterranean European populations.

REVISTA / JOURNAL:  - Semin Arthritis Rheum 2002 Jun;31(6):361-70.

AUTORES / AUTHORS:  - Ioannidis JP; Tarassi K; Papadopoulos IA; Voulgari PV; Boki KA; Papasteriades CA; Drosos AA

INSTITUCIÓN / INSTITUTION:  - Clinical and Molecular Epidemiology Unit, Department of Hygiene and Epidemiology and the Division of Rheumatology, University of Ioannina School of Medicine, Ioannina, Greece.

RESUMEN / SUMMARY:  - OBJECTIVES: To assess the strength of the associations between HLA shared epitopes (SE) and rheumatoid arthritis (RA) susceptibility, articular disease severity, and extra-articular features in Mediterranean European populations. METHODS: One hundred and seventy-four Greek RA patients and 103 controls were evaluated. Data were then included in a meta-analysis of 9 studies of Mediterranean European populations (959 RA patients and 1,405 controls). RESULTS: In our study population, SE alleles were significantly more common in RA patients than in controls (odds ratio [OR], 2.5; 95% confidence interval [CI], 1.4-4.3). Larsen radiologic score was predicted by SE and disease duration. SE did not increase the risk of any extra-articular manifestation. The meta-analysis showed a pooled OR of 3.7 (95% CI, 2.6-5.2) for susceptibility to RA conferred by SE (OR, 3.4 v 3.9 in Greek v non-Greek populations). CONCLUSIONS: SE determine articular destruction without increasing the risk of extra-articular manifestations. The immunogenetic associations of RA susceptibility are consistent, but their strength may depend on the SE prevalence in different ethnic groups.

 

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[154]

TÍTULO / TITLE:  - Heat shock proteins, anti-heat shock protein reactivity and allograft rejection.

REVISTA / JOURNAL:  - Transplantation 2001 Jun 15;71(11):1503-7.

AUTORES / AUTHORS:  - Pockley AG

INSTITUCIÓN / INSTITUTION:  - Division of Clinical Sciences (NGH), Clinical Sciences Centre, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK.

RESUMEN / SUMMARY:  - Heat shock proteins are families of highly conserved immunodominant molecules, reactivity to which has been implicated in the pathogenesis of a number of autoimmune and vascular disease states. However, heat shock proteins are cytoprotective, and in clinical and experimental arthritis, anti-heat shock protein reactivity can down modulate immune responses via a self-Hsp reactive, Th2-type mechanism. Despite a number of studies associating heat shock protein expression and anti-heat shock protein reactivity with allograft rejection, the balance between protective and damaging effects and the precise influence of these responses on graft outcome is unclear. This article reviews current knowledge surrounding heat shock proteins, autoimmunity, and allograft rejection and presents a perspective on the potential influence of these proteins and the stress response on allograft outcome.  N. Ref:: 90

 

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[155]

TÍTULO / TITLE:  - Infliximab as disease-modifying therapy.

REVISTA / JOURNAL:  - Eur J Gastroenterol Hepatol 2003 Mar;15(3):233-7.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.meg.0000049995.68425.94

AUTORES / AUTHORS:  - D’Haens GR

INSTITUCIÓN / INSTITUTION:  - Department of Gastroenterology, University Hospital Gasthuisberg, Leuven, Belgium. geert.dhaens@uz.kuleuven.ac.be

RESUMEN / SUMMARY:  - Recent insights in the pathophysiology of Crohn’s disease have revealed that tumour necrosis factor (TNF) plays a pivotal role in mucosal inflammation. Infliximab is a chimeric anti-TNF monoclonal antibody with potent anti-inflammatory effects, probably based on apoptosis of inflammatory cells. Numerous controlled trials have demonstrated efficacy in both active and fistulating Crohn’s disease. Appropriate indications for using infliximab and growing experience with safety aspects have made this treatment a highly valuable tool in the management of Crohn’s disease.  N. Ref:: 31

 

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[156]

TÍTULO / TITLE:  - Prevention of and treatment for hepatitis B virus infection after liver transplantation in the nucleoside analogues era.

REVISTA / JOURNAL:  - Am J Transplant 2003 Mar;3(3):250-8.

AUTORES / AUTHORS:  - Papatheodoridis GV; Sevastianos V; Burroughs AK

INSTITUCIÓN / INSTITUTION:  - 2nd Academic Department of Medicine, Hippokration General Hospital, Athens, Greece. gpapath@cc.uoa.gr

RESUMEN / SUMMARY:  - Post-transplant prophylaxis with hepatitis B immune globulin (HBIG) has significantly reduced hepatitis B virus (HBV) recurrence rates, but it is rather ineffective in patients with pretransplant viremia. Moreover, long-term HBIG administration is very expensive and may be associated with emergence of escape HBV mutants. Lamivudine has been widely used in the management of HBV transplant patients. Pretransplant lamivudine lowers HBV viremia, decreasing the risk of post-transplant HBV recurrence, but to try and minimize development of resistant HBV strains, it should start within the last 6 months of the anticipated transplantation timing. Preemptive post-transplant lamivudine monotherapy is associated with progressively increasing HBV recurrence rates, but combined therapy with lamivudine and HBIG at relatively low dosage is currently the most effective approach in this setting, even in HBV-DNA-positive patients, who also receive lamivudine in the pretransplant period. The most frequent therapy for post-transplant HBV recurrence is lamivudine, but the increasing resistance rates represent a rather challenging problem. Adefovir dipivoxil and entecavir are currently the most promising agents for lamivudine-resistant HBV strains. All these advances in anti-HBV therapy have made HBV liver disease an indication for liver transplantation irrespective of viral replication status, a complete turn around from 10 years ago.  N. Ref:: 93

 

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[157]

TÍTULO / TITLE:  - Treatment of the bleeding inhibitor patient.

REVISTA / JOURNAL:  - Semin Thromb Hemost 2003 Feb;29(1):77-86.

      ●● Enlace al texto completo (gratuito o de pago) 1055/s-2003-37972

AUTORES / AUTHORS:  - Astermark J

INSTITUCIÓN / INSTITUTION:  - Department of Coagulation Disorders, Malmo University Hospital, Malmo, Sweden. Jan.astermark@medforsk.mas.lu.se

RESUMEN / SUMMARY:  - The development of inhibitory antibodies to factor (F) VIII and FIX continues to be a major challenge in the treatment of patients with hemophilia. In patients with low-responding inhibitors, it is usually possible to saturate the inhibitor with the deficient factor and to achieve hemostasis, but in patients with high-responding inhibitors, two major tasks have to be considered. One is how to treat the acute bleedings and the other is how to permanently eliminate the immune response, in other words, to induce tolerance. There are several hemostatic agents available for bleeding patients with high-responding inhibitors. Nonactivated and activated prothrombin complex concentrates (PCCs) have been used for almost 30 years, and since the beginning of the 1980s, porcine FVIII has also been used. In more recent years, recombinant FVIIa has been added to the therapeutic armamentarium and has been shown to control hemostasis in most patients. Immunoadsorption may temporarily reduce the inhibitor, enabling replacement therapy for several days. Available data on these alternative regimens will be discussed with a focus on the mechanisms of action, pharmacokinetics, safety, monitoring, and clinical experience.  N. Ref:: 76

 

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[158]

TÍTULO / TITLE:  - Use of the A(2A) adenosine receptor as a physiological immunosuppressor and to engineer inflammation in vivo.

REVISTA / JOURNAL:  - Biochem Pharmacol 2003 Feb 15;65(4):493-501.

AUTORES / AUTHORS:  - Sitkovsky MV

INSTITUCIÓN / INSTITUTION:  - Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1892, USA. mvsitkov@helix.nih.gov

RESUMEN / SUMMARY:  - Inflammation must be inhibited in order to treat, e.g., sepsis or autoimmune diseases or must be selectively enhanced to improve, for example, immunotherapies of tumors or the development of vaccines. Predictable enhancement of inflammation depends upon the knowledge of the “natural” pathways by which it is down-regulated in vivo. Extracellular adenosine and A(2A) adenosine (purinergic) receptors were identified recently as anti-inflammatory signals and as sensors of excessive inflammatory tissue damage, respectively (Ohta A and Sitkovsky M, Nature 2001;414:916-20). These molecules may function as an important part of a physiological “metabolic switch” mechanism, whereby the inflammatory stimuli-produced local tissue damage and hypoxia cause adenosine accumulation and signaling through cyclic AMP-elevating A(2A) adenosine receptors in a delayed negative feedback manner. Patterns of A(2A) receptor expression are activation- and differentiation-dependent, thereby allowing for the “acquisition” of an immunosuppressive “OFF button” and creation of a time-window for immunomodulation. Identification of A(2A) adenosine receptors as “natural” brakes of inflammation provided a useful framework for understanding how tissues regulate inflammation and how to enhance or decrease (engineer) inflammation by targeting this endogenous anti-inflammatory pathway. These findings point to the need of more detailed testing of anti-inflammatory agonists of A(2A) receptors and create a previously unrecognized strategy to enhance inflammation and targeted tissue damage by using antagonists of A(2A) receptors. It is important to further identify the contributions of different types of immune cells at different stages of the inflammatory processes in different tissues to enable the “tailored” treatments with drugs that modulate the signaling through A(2A) purinergic receptors.  N. Ref:: 113

 

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[159]

TÍTULO / TITLE:  - Mechanisms of corticosteroid resistance in rheumatoid arthritis: a putative role for the corticosteroid receptor beta isoform.

REVISTA / JOURNAL:  - Ann N Y Acad Sci 2002 Jun;966:39-48.

AUTORES / AUTHORS:  - Chikanza IC

INSTITUCIÓN / INSTITUTION:  - Bone and Joint Research Unit, John Vane Building, St. Bartholomew’s and Royal London School of Medicine, Charterhouse Square, London, United Kingdom. i.c.chikanza@gmul.uk

RESUMEN / SUMMARY:  - Corticosteroids (CSs) have potent immunosuppressive effects and are commonly used to treat a range of immunological and inflammatory diseases such as rheumatoid arthritis (RA). These effects are mediated by the ability of CSs to modulate gene expression. CSs act by binding to the CS receptor (CR), which exists as alpha and beta isoforms. Only CRalpha binds CS. CRbeta functions as an endogenous inhibitor of CS and is expressed in several tissues. The CS/CRalpha complex binds to the glucocorticosteroid response element in the nucleus and also interferes with AP-1 and NF-kappaB binding. Thus, CSs inhibit the transcription of AP-1 and NF-kappaB inducible genes, such as interleukin (IL)-2, IL-6, IL-8, IL-1beta, and tumor necrosis factor (TNF) alpha, as well as T-cell proliferation. In clinical practice, a proportion of RA patients do not respond adequately to CS therapy. On this basis, RA patients can be divided on clinical grounds and on the ability of CSs to inhibit concanavalin A (conA)-induced peripheral blood T-cell proliferation in vitro into CS-sensitive (SS) and CS-resistant (SR) subgroups. The in vitro defined SS and SR subgroups correlate with the clinical responses to CS therapy. The mechanisms of the SR in RA patients remain unknown but may include the following: dysregulation of CRalpha function, alterations in the intracellular signaling mechanisms and/or utilization of various other cellular activation pathways, perturbations of the cytokine milieu, and inhibition of lipocortin. In SR subjects, CSs fail to significantly inhibit conA-induced IL-2 and IL-4 secretion and LPS-induced IL-8, IL-1beta secretion in vitro. CS therapy fails to reduce the circulating levels of IL-8, IL-1beta, and TNFalpha in SR RA patients. Peripheral blood mononuclear cells (PBMCs) from SR significantly overexpress activated NF-kappaB and IkappaBalpha. In vitro CSs fail to significantly inhibit conA-induced NF-kappaB activation in PBMCs from SR RA patients. Our preliminary observations show enhanced CRbeta expression by PBMCs from SR RA patients. It is most likely that other molecular mechanisms such as enhanced AP-1 expression are involved, and we currently are investigating such possibilities.  N. Ref:: 60

 

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[160]

TÍTULO / TITLE:  - The role of monoclonal antibodies in stem cell transplantation.

REVISTA / JOURNAL:  - Semin Oncol 2004 Feb;31(1):83-9.

AUTORES / AUTHORS:  - Wasil T; Rai KR; Mehrotra B

INSTITUCIÓN / INSTITUTION:  - Long Island Jewish Medical Center, Division of Hematology-Oncology, New Hyde Park, NY 11040, USA.

RESUMEN / SUMMARY:  - Monoclonal antibodies directed at the lymphoid antigens have become established treatments for hematological malignancies either alone or in combination with chemotherapy. However, their incorporation in the transplant setting remains investigational. This review focuses on the currently available data for in vitro and in vivo purging with these antibodies as well as their role in modulating graft-versus-host disease (GVHD).  N. Ref:: 44

 

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[161]

TÍTULO / TITLE:  - Weakness of respiratory and skeletal muscles after a short course of steroids in patients with acute lung rejection.

REVISTA / JOURNAL:  - Eur Respir J 2002 Aug;20(2):497-9.

AUTORES / AUTHORS:  - Nava S; Fracchia C; Callegari G; Ambrosino N; Barbarito N; Felicetti G

INSTITUCIÓN / INSTITUTION:  - Respiratory Unit, Istituto Scientifico di Montescano, Pavia, Italy. snava@fsm.it

RESUMEN / SUMMARY:  - There have been occasional reports of acute respiratory and skeletal muscle weakness in intensive care unit patients treated with massive doses of corticosteroids. However, in this setting the concomitant use of other drugs may have influenced the finding. In this study the effects of 5 days of treatment with high doses of steroids in consecutive patients with acute lung rejection after transplantation were systematically evaluated. Maximal inspiratory pressure during phrenic nerve stimulation and peak torque of isokinetic contraction of the quadriceps and hamstring muscles were measured objectively. Compared to the pretreatment condition, approximately 45% of patients showed acute generalised muscle weakness that recovered after approximately 2 months. This demonstrates muscle weakness induced by steroids within patients.  N. Ref:: 6

 

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[162]

TÍTULO / TITLE:  - Calcineurin inhibitor-free CD28 blockade-based protocol protects allogeneic islets in nonhuman primates.

REVISTA / JOURNAL:  - Diabetes. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://diabetes.diabetesjournals.org/ 

      ●● Cita: Diabetes: <> 2002 Feb;51(2):265-70.

AUTORES / AUTHORS:  - Adams AB; Shirasugi N; Durham MM; Strobert E; Anderson D; Rees P; Cowan S; Xu H; Blinder Y; Cheung M; Hollenbaugh D; Kenyon NS; Pearson TC; Larsen CP

INSTITUCIÓN / INSTITUTION:  - Emory Transplant Center, Department of Surgery, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

RESUMEN / SUMMARY:  - Recent success using a steroid-free immunosuppressive regimen has renewed enthusiasm for the use of islet transplantation to treat diabetes. Toxicities associated with the continued use of a calcineurin inhibitor may limit the wide-spread application of this therapy. Biological agents that block key T-cell costimulatory signals, in particular the CD28 pathway, have demonstrated extraordinary promise in animal models. LEA29Y (BMS-224818), a mutant CTLA4-Ig molecule with increased binding activity, was evaluated for its potential to replace tacrolimus and protect allogeneic islets in a preclinical primate model. Animals received either the base immunosuppression regimen (rapamycin and anti-IL-2R monoclonal antibody [mAb]) or the base immunosuppression and LEA29Y. Animals receiving the LEA29Y/rapamycin/anti-IL-2R regimen (n = 5) had significantly prolonged islet allograft survival (204, 190, 216, 56, and >220 days). In contrast, those animals receiving the base regimen alone (n = 2) quickly rejected the transplanted islets at 1 week (both at 7 days). The LEA29Y-based regimen prevented the priming of anti-donor T- and B-cell responses, as detected by interferon-gamma enzyme-linked immunospot and allo-antibody production, respectively. The results of this study suggest that LEA29Y is a potent immunosuppressant that can effectively prevent rejection in a steroid-free immunosuppressive protocol and produce marked prolongation of islet allograft survival in a preclinical model.

 

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[163]

TÍTULO / TITLE:  - Nutrient signaling through TOR kinases controls gene expression and cellular differentiation in fungi.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2004;279:53-72.

AUTORES / AUTHORS:  - Rohde JR; Cardenas ME

INSTITUCIÓN / INSTITUTION:  - Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.

RESUMEN / SUMMARY:  - The TOR kinases were first identified in Saccharomyces cerevisiae as the targets of the immunosuppressive drug rapamycin. Subsequent studies employing rapamycin as a tool in yeast have given us insight into the structure and function of the TOR kinases, as well as the biological role of the TOR signaling program in transmitting nutrient signals to promote cell growth. One of the major advances from this area has been in defining an unexpected role for TOR signaling in the regulation of transcription. The identification of target genes subject to regulation by TOR has provided a platform for the dissection of the signaling events downstream of the TOR kinases. Studies aimed at understanding TOR-regulated transcription have begun to shed light on how TOR signaling cooperates with other signaling programs. In addition, the TOR pathway regulates the developmental program of pseudohyphal differentiation in concert with highly conserved MAP kinase and PKA signaling programs. Remarkably, rapamycin also blocks filamentation in a number of important human and plant pathogens and the mechanism of rapamycin action is conserved in Candida albicans and Cryptococcus neoformans. The antimicrobial properties of less immunosuppressive analogs of rapamycin hold promise for the development of an effective antifungal therapy.  N. Ref:: 65

 

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[164]

TÍTULO / TITLE:  - Signaling pathways involved in translational control of protein synthesis in skeletal muscle by leucine.

REVISTA / JOURNAL:  - J Nutr. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.nutrition.org/ 

      ●● Cita: Journal of Nutrition: <> 2001 Mar;131(3):856S-860S.

AUTORES / AUTHORS:  - Anthony JC; Anthony TG; Kimball SR; Jefferson LS

INSTITUCIÓN / INSTITUTION:  - Department of Cellular and Molecular Physiology, P.O. Box 850, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

RESUMEN / SUMMARY:  - Numerous reports established that in skeletal muscle the indispensable branched-chain amino acid leucine is unique in its ability to initiate signal transduction pathways that modulate translation initiation. Oral administration of leucine stimulates protein synthesis in association with hyperphosphorylation of the translational repressor, eukaryotic initiation factor (eIF) 4E binding protein 1 (4E-BP1), resulting in enhanced availability of the mRNA cap-binding protein eIF4E, for binding eIF4G and forming the active eIF4F complex. In addition, leucine enhances phosphorylation of the 70-kDa ribosomal protein S6 kinase (S6K1). These results suggest that leucine upregulates protein synthesis in skeletal muscle by enhancing both the activity and synthesis of proteins involved in mRNA translation. The stimulatory effects of leucine on translation initiation are mediated in part through the protein kinase mammalian target of rapamycin (mTOR), where both insulin signaling and leucine signaling converge to promote a maximal response.  N. Ref:: 34

 

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[165]

TÍTULO / TITLE:  - The HCMV gene products US2 and US11 target MHC class I molecules for degradation in the cytosol.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2002;269:37-55.

AUTORES / AUTHORS:  - van der Wal FJ; Kikkert M; Wiertz E

INSTITUCIÓN / INSTITUTION:  - Department of Medical Microbiology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.

RESUMEN / SUMMARY:  - Over millions of years of coevolution with their hosts, viruses have developed highly effective strategies to elude the host immune system. The degradation of major histocompatibility complex (MHC) class I heavy chains by human cytomegalovirus (HCMV) is an example of this. Two HCMV proteins, US2 and US11, target newly synthesized MHC class I heavy chains for destruction via a pathway that involves ubiquitin-dependent retrograde transport, or “dislocation”, of the heavy chains from the ER to the cytosol, where the proteins are degraded by proteasomes. In this review, US2- and US11-mediated degradation of MHC class I heavy chains is discussed in relation to data concerning the degradation of other ER luminal proteins. A new, unified model for translocon-facilitated dislocation and degradation of MHC class I heavy chains is presented.  N. Ref:: 115

 

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[166]

TÍTULO / TITLE:  - Beta-herpesvirus challenges in the transplant recipient.

REVISTA / JOURNAL:  - J Infect Dis. Acceso gratuito al texto completo a partir de los 2 años de la publicación;  - http://www.journals.uchicago.edu/ 

      ●● Cita: J. of Infectious Diseases: <> 2002 Oct 15;186 Suppl 1:S99-S109.

AUTORES / AUTHORS:  - Ljungman P

INSTITUCIÓN / INSTITUTION:  - Karolinska Institutet, SE-14186 Stockholm, Sweden. per.ljungman@medhs.ki.se

RESUMEN / SUMMARY:  - Cytomegalovirus (CMV) has major consequences after allogeneic stem cell and solid organ transplantation. CMV may cause significant morbidity and mortality, and monitoring to detect reactivation to reduce disease or management of end organ disease is associated with increased resource utilization. Two other members of the beta-herpesvirus family, human herpesvirus (HHV) type 6 and HHV-7, are increasingly recognized as important pathogens in transplant recipients, either by direct infection (e.g., encephalitis, hepatitis, or pneumonitis) or via interaction with CMV. In addition to direct effects of CMV infection, such indirect effects as an increased risk for bacterial and fungal infections or impaired graft acceptance and function are important research topics. Diagnosis and treatment of CMV infection is currently more advanced than for HHV-6 and HHV-7.  N. Ref:: 109

 

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[167]

TÍTULO / TITLE:  - A benefit-risk assessment of basiliximab in renal transplantation.

REVISTA / JOURNAL:  - Drug Saf. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.csmwm.org/ 

      ●● Cita: Drug Safety: <> 2004;27(2):91-106.

AUTORES / AUTHORS:  - Boggi U; Danesi R; Vistoli F; Del Chiaro M; Signori S; Marchetti P; Del Tacca M; Mosca F

INSTITUCIÓN / INSTITUTION:  - Division of General Surgery and Transplants, Department of Oncology, Transplants and Advanced Technologies in Medicine, University of Pisa, Pisa, Italy. uboggi@med.unipi.it

RESUMEN / SUMMARY:  - Interleukin-2 (IL-2) and its receptor (IL-2R) play a central role in T lymphocyte activation and immune response after transplantation. Research on the biology of IL-2R allowed the identification of key signal transduction pathways involved in the generation of proliferative and antiapoptotic signals in T cells. The alpha-chain of the IL-2R is a specific peptide against which monoclonal antibodies have been raised, with the aim of blunting the immune response by means of inhibiting proliferation and inducing apoptosis in primed lymphocytes. Indeed, basiliximab, one of such antibodies, has proved to be effective in reducing the episodes of acute rejection after kidney and pancreas transplantation. The use of basiliximab was associated with a significant reduction in the incidence of any treated rejection episodes after kidney transplantation in the two major randomised studies (placebo 52.2% vs basiliximab 34.2% at 6 months, European study; placebo 54.9% vs basiliximab 37.6% at 1 year, US trial). Basiliximab and equine antithymocyte globulin (ATG) administration resulted in a similar rate of biopsy-proven acute rejection at 6 months (19% for both) and at 12 months (19% and 20%, respectively). The use of basiliximab appears not to be associated with an increased incidence of adverse events as compared with placebo in immunosuppressive regimens, including calcineurin inhibitors, mycophenolate mofetil or azathioprine and corticosteroids, and its safety profile is superior to ATG. Moreover, a similar occurrence of infections is noted in selected studies (65.5% after basiliximab vs 65.7% of controls), including cytomegalovirus infection (17.3% vs 14.5%), and cytokine-release syndrome is not observed. Finally, economic analysis demonstrated lower costs of overall treatment in patients treated with basiliximab. Therefore, the use of basiliximab entails a very low risk, allows safe reduction of corticosteroid dosage and reduces the short- and mid-term rejection rates. However, the improvement in the long-term survival of kidney grafts in patients treated according to modern immunosuppressive protocols is still to be demonstrated. These conclusions are based on a systematic review of the scientific literature, indexed on Medline database, concerning the mechanism of action, therapeutic activity, safety and pharmacoeconomic evaluation of basiliximab in renal transplantation.  N. Ref:: 62

 

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[168]

TÍTULO / TITLE:  - Cardiac allograft vasculopathy and dysregulation of the NO synthase pathway.

REVISTA / JOURNAL:  - Arterioscler Thromb Vasc Biol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://atvb.ahajournals.org/ 

      ●● Cita: Arteriosclerosis & Thrombosis :  A J. Vasc Biol: <> 2003 Apr 1;23(4):567-75. Epub 2003 Mar 20.

      ●● Enlace al texto completo (gratuito o de pago) 1161/01.ATV.0000067060.31369.F9

AUTORES / AUTHORS:  - Weis M; Cooke JP

INSTITUCIÓN / INSTITUTION:  - Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, Calif 94305-5406, USA.

RESUMEN / SUMMARY:  - Cardiac allograft vasculopathy is the most aggressive form of atherosclerosis in humans and is the leading cause of death after the first year of heart transplantation. Endothelial dysfunction is a major contributing factor to the acceleration of coronary vascular disease in these individuals. A reflection of this endothelial dysfunction is the severe impairment in endothelium-dependent vasodilation that occurs early after transplantation. The etiology of this allograft endothelial alteration is multifactorial and may include preexisting atherosclerosis of the graft vessels, reperfusion injury during transplantation, denervation, disruption of the lymphatic system, and acute and chronic immune injury, as well as traditional risk factors for coronary artery disease (hyperlipidemia, diabetes, hypertension, or hyperhomocysteinemia) and pathogens, such as cytomegalovirus. The alteration in endothelial function affects vasomotor tone of the coronary arteries. Evidence indicates that there may be an impairment of endothelial production and/or activity of NO. Because NO is a potent vasodilator, its deficiency would explain the abnormal vasomotor tone in these individuals. In addition, because NO inhibits key processes in vascular inflammation and atherosclerosis, its absence may contribute to the acceleration of transplant vascular disease. Recent studies from our group and others have shed light on the mechanisms of endothelial dysfunction and its importance in cardiac allograft vasculopathy. In addition, the alteration in endothelial function contributes to vascular inflammation and progression of the disease.  N. Ref:: 148

 

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[169]

TÍTULO / TITLE:  - The effect of hematopoietic growth factors on the risk of graft-vs-host disease after allogeneic hematopoietic stem cell transplantation: a meta-analysis.

REVISTA / JOURNAL:  - Bone Marrow Transplant 2003 Oct;32(8):771-5.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.bmt.1704228

AUTORES / AUTHORS:  - Ho VT; Mirza NQ; Junco Dd D; Okamura T; Przepiorka D

INSTITUCIÓN / INSTITUTION:  - Baylor College of Medicine, Center for Cell and Gene Therapy, Houston, TX, USA.

RESUMEN / SUMMARY:  - The effect of hematopoietic growth factors on neutrophil recovery after allogeneic transplantation is well-recognized. Recent laboratory studies demonstrated that these cytokines may also modify T-cell and dendritic cell function, but whether the effect is strong enough to alter the risk of GVHD is unclear. We performed a meta-analysis to determine the effect of G-CSF or GM-CSF on the risk of nonhematopoietic outcomes after allogeneic transplantation. A search of the literature from 1986 to present yielded 18 publications in which data were provided for cohorts receiving growth factor vs either placebo or no therapy. These included nine prospective randomized studies, eight retrospective cohort studies, and one case-control study comprising a total of 1198 patients. The publication types were heterogeneous with regard to demographic and treatment characteristics, although within publications, comparative groups were generally balanced. The pooled risk ratio estimates with use of growth factor was 1.08 (95% CI 0.87-1.33, P=0.48) for grades 2-4 acute GVHD, 1.22 (95% CI 0.80-1.86, P=0.99) for grades 3-4 acute GVHD, and 1.02 (95% CI 0.82-1.26, P=0.87) for chronic GVHD. This analysis did not detect a significant change in the risk of acute or chronic GVHD after allogeneic hematopoietic stem cell transplantation when hematopoietic growth factors were used to shorten the initial period of neutropenia.

 

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[170]

TÍTULO / TITLE:  - Self major histocompatibility complex class-II-specific regulatory CD4 T cells prevent both Th1- and Th2-mediated autoimmune diseases in the rat.

REVISTA / JOURNAL:  - Microbes Infect 2001 Sep;3(11):955-60.

AUTORES / AUTHORS:  - Pelletier L; Savignac M; Xystrakis E; Duplan V; Druet P; Abdelhadi S

INSTITUCIÓN / INSTITUTION:  - Inserm U28, Hopital Purpan, place du D Baylac, 31059, Toulouse, France. Lucette.Pelletier@purpan.inserm.fr

RESUMEN / SUMMARY:  - It is clear that functional heterogeneity of T cells may be explained by differential cytokine production. The aim of this paper was to review evidence for regulatory cells, generated after HgCl(2)-exposure. They differ from classical Th1 and Th2 cells, produce transforming growth factor-beta and interleukin-10 and exert their regulatory functions in a Th1/Th2-unrestricted fashion.  N. Ref:: 46

 

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[171]

TÍTULO / TITLE:  - Role of leucine in the regulation of mTOR by amino acids: revelations from structure-activity studies.

REVISTA / JOURNAL:  - J Nutr. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.nutrition.org/ 

      ●● Cita: Journal of Nutrition: <> 2001 Mar;131(3):861S-865S.

AUTORES / AUTHORS:  - Lynch CJ

INSTITUCIÓN / INSTITUTION:  - Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA. clynch@psu.edu

RESUMEN / SUMMARY:  - In this study an overview is presented of the mTOR signaling pathway and its regulation by amino acids, particularly L-leucine. Our laboratory is studying amino acid regulation of mTOR in adipocytes. Potential roles for mTOR in adipocytes that were previously posited include hypertrophic growth, leptin secretion, protein synthesis and adipose tissue morphogenesis. A current area of interest in the field is how amino acids regulate mTOR and which amino acids are regulatory. Revelations concerning mechanism and recognition are emerging from different laboratories that examined the structural requirements for stimulation and inhibition of the mTOR signaling pathway by leucine and amino acid analogs. In adipocytes and some other cell types, leucine appears to be the main regulatory amino acid. However, this is not uniformly the case. In those cells where mTOR is regulated by several amino acids, there is evidence that the mechanism of mTOR activation may be different from cells where mainly leucine is regulatory. Furthermore, in tissues where leucine regulates mTOR, the possible existence of different tissue-specific leucine recognition sites may be indicated.  N. Ref:: 47

 

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[172]

TÍTULO / TITLE:  - Drug-eluting stents and glycoprotein IIb/IIIa inhibitors: combination therapy for the future.

REVISTA / JOURNAL:  - Am Heart J 2003 Oct;146(4 Suppl):S13-7.

      ●● Enlace al texto completo (gratuito o de pago) 1016/j.ahj.2003.09.004

AUTORES / AUTHORS:  - Leon MB; Bakhai A

RESUMEN / SUMMARY:  - BACKGROUND: Although coronary stenting has improved the results of coronary interventions compared to coronary angioplasty alone, in-stent restenosis remains a significant limitation of this procedure. Drug-eluting stents with or without glycoprotein IIb/IIIa inhibitor therapy represent an additional advance in the evolution of this strategy. METHODS: We review the currently available trials comparing studies of non-drug-eluting and drug-eluting stents using sirolimus and paclitaxel agents and their derivatives. RESULTS: Ten studies are available that compare drug-eluting to traditional non-drug-eluting stents. A variety of antiplatelet regimes have been used. The majority of these studies are in the process of being published. No head-to-head studies comparing different drug-eluting stents are available. CONCLUSIONS: Drug-eluting stents using sirolimus and paclitaxel in combination with enhanced antiplatelet strategies represent an important advantage over non-drug-eluting stents for the reduction of in-stent restenosis. The rate at which drug-eluting stents are adapted into widespread practice depends heavily on whether they are safe, efficacious, and cost-effective in various clinical settings.  N. Ref:: 28

 

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[173]

TÍTULO / TITLE:  - Targeted therapy of solid malignancies via HLA class II antigens: a new biotherapeutic approach?

REVISTA / JOURNAL:  - Oncogene 2003 Sep 29;22(42):6564-9.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.onc.1206960

AUTORES / AUTHORS:  - Altomonte M; Fonsatti E; Visintin A; Maio M

INSTITUCIÓN / INSTITUTION:  - Cancer Bioimmunotherapy Unit, Department of Medical Oncology, Centro di Riferimento Oncologico, Istituto di Ricovero e Cura a Carattere Scientifico, via Pedemontana Occ. le, 12, Aviano 33081, Italy. maltomonte@cro.it

RESUMEN / SUMMARY:  - Intracellular signals, delivered in professional antigen-presenting cells following the engagement of major histocompatibility complex (MHC) class II molecules, activate a variety of cellular functions that also contribute to efficient antigen presentation. As far as human malignancies, the signaling ability of human leukocyte antigens (HLA) class II molecules is a rather well-characterized event in hematologic tumors; in contrast, very limited evidences are available in solid neoplasias of different histotypes that may constitutively express HLA class II antigens. Among solid malignancies, a significant proportion of human cutaneous melanomas have been shown to express HLA class II molecules, and cutaneous melanoma undoubtedly represents a ‘model disease’ to investigate tumor immunobiology, to unveil the molecular basis underlying the interactions between neoplastic cells and host’s immune system, and ultimately to set up new bio-immunotherapeutic approaches. Upcoming preclinical evidences unveil a signaling potential of HLA-DR antigens expressed on melanoma cells, and suggest for the clinical implication of HLA class II molecules as novel therapeutic targets. Therefore, in this review, we will focus on the emerging role of HLA class II antigens as intracellular signal transducing elements in neoplastic cells of the melanocytic lineage, emphasizing their foreseeable role in targeted therapy of human melanoma and potentially of HLA class II antigens-positive tumors of different histology.  N. Ref:: 94

 

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[174]

TÍTULO / TITLE:  - Cellular engineering of HSV-tk transduced, expanded T lymphocytes for graft-versus-host disease management.

REVISTA / JOURNAL:  - Acta Haematol 2003;110(2-3):121-31.

      ●● Enlace al texto completo (gratuito o de pago) 1159/000072461

AUTORES / AUTHORS:  - Burger SR; Kadidlo DM; Basso L; Bostrom N; Orchard PJ

INSTITUCIÓN / INSTITUTION:  - Advanced Cell and Gene Therapy, Chapel Hill, NC 27516, USA. sburger@ac-gt.com

RESUMEN / SUMMARY:  - Engineering donor T lymphocytes with inducible ‘suicide genes’, such as herpes simplex virus thymidine kinase, has potential to improve safety and efficacy in allogeneic transplantation by facilitating management of graft-versus-host disease. Elective administration of a relatively nontoxic pro-drug would induce in vivo negative selection of engineered lymphocytes specifically, sparing other donor hematopoietic cells. The engineered cells must retain immunologic function, and undergo negative selection in response to clinically attainable plasma concentrations of pro-drug. The cell engineering process itself, typically involving activation, transduction, ex vivo expansion, and selection, must produce clinically useful numbers of genetically modified cells at high purity. We discuss development of a cellular engineering manufacturing process that yields transduced, expanded T lymphocytes meeting these requirements.  N. Ref:: 37

 

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[175]

TÍTULO / TITLE:  - Hereditary hemochromatosis: perspectives of public health, medical genetics, and primary care.

REVISTA / JOURNAL:  - Genet Med 2003 Jan-Feb;5(1):1-8.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.GIM.0000047946.94270.34

AUTORES / AUTHORS:  - Imperatore G; Pinsky LE; Motulsky A; Reyes M; Bradley LA; Burke W

INSTITUCIÓN / INSTITUTION:  - National Canter for Chronic Disease Prevention and Health Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA.

RESUMEN / SUMMARY:  - Hereditary hemochromatosis (HHC) is a condition characterized by excess iron in body tissues, resulting in complications such as cirrhosis, cardiomyopathy, diabetes, and arthritis. These complications usually manifest during adulthood. Two methods of screening for the detection of early stage of HHC are available: serum iron measures and molecular testing to detect mutations in the gene. These phenotypic and genotypic screening tests are of particular interest because a simple treatment-periodic phlebotomy-can be used to prevent iron accumulation and clinical complications. HHC might represent the first adult-onset genetic disorder for which universal population-based screening would be appropriate. Therefore, HHC has been proposed as a paradigm for the introduction of adult genetic diseases into clinical and public health practice. However, universal screening for HHC has not been recommended because of the uncertainty about the natural history of the iron overload or HHC and, in particular, uncertainty about the prevalence of asymptomatic iron overload and the likelihood that it will progress to clinical complications. If universal screening is not appropriate based on current data, what other measures might reduce the disease burden of iron overload? New studies provide more systematic information about the penetrance of the C282Y mutation and shed further light on the natural history of the disorder. The authors review these data and consider their implications for public health, medical genetics, and primary care.  N. Ref:: 64

 

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[176]

TÍTULO / TITLE:  - B-cell activation and lymphoma in patients with HIV.

REVISTA / JOURNAL:  - Curr Opin Oncol 2002 Sep;14(5):528-32.

AUTORES / AUTHORS:  - Martinez-Maza O; Breen EC

INSTITUCIÓN / INSTITUTION:  - Department of Microbiology, David Geffen School of Medicine, University of California-Los Angeles, 90095, USA. omartinez@mednet.ucla.edu

RESUMEN / SUMMARY:  - The risk of developing non-Hodgkin lymphoma (AIDS lymphoma) is greatly increased in HIV infection. Disruption of immune function by HIV infection may contribute to lymphomagenesis by inducing (1) loss of immunoregulation of Epstein-Barr virus-infected B cells [immunoblastic and central nervous system (CNS) lymphoma] caused by loss of T-cell function, and (2) chronic B-cell hyperactivation enhancing the generation of genetic lesions (c- :immunoglobulin gene translocation, -6 overexpression) associated with some forms of AIDS lymphoma (Burkitt lymphoma-like small noncleaved cell lymphoma and large noncleaved cell lymphoma). Also, the overproduction of B-cell-stimulatory cytokines (interleukin 10 and 6) has the potential to contribute to tumor development by supporting the growth and viability of nascent lymphoma cell clones. Therefore, HIV infection-associated B-cell hyperactivation, including direct activation of B cells by various mechanisms, and chronic overproduction of B-cell-stimulatory cytokines have the potential to contribute to the development and growth of AIDS lymphoma. Several recent reports are discussed in this review, including recent work relevant to understanding the potential of a virus-encoded cytokine-like molecule, HHV8 vIL6, to induce B-cell hyperactivation in HIV-infected people, work pointing to the potential role of a chemokine (stromal cell-derived factor 1) in lymphomagenesis, and studies on phenotypic changes in circulating B cells in HIV infection.  N. Ref:: 38

 

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[177]

TÍTULO / TITLE:  - New therapies for ankylosing spondylitis: etanercept, thalidomide, and pamidronate.

REVISTA / JOURNAL:  - Rheum Dis Clin North Am 2003 Aug;29(3):481-94, viii.

AUTORES / AUTHORS:  - Davis JC Jr; Huang F; Maksymowych W

INSTITUCIÓN / INSTITUTION:  - Division of Rheumatology, Department of Medicine, University of California-San Francisco, 533 Parnassus Avenue, Box 0633, San Francisco, CA 94143, USA. jdavis@medicine.ucsf.edu

RESUMEN / SUMMARY:  - Ankylosing spondylitis (AS) is the most common of a group of diseases called seronegative spondyloarthropathies. This group of diseases shares common demographic, clinical, and genetic features. This article reviews the rationale, clinical efficacy, and safety reports of etanercept, thalidomide, and pamidronate in the treatment of patients who have AS.  N. Ref:: 48

 

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[178]

TÍTULO / TITLE:  - Signal transduction via MHC class I molecules in endothelial and smooth muscle cells.

REVISTA / JOURNAL:  - Crit Rev Immunol 2003;23(1-2):109-28.

AUTORES / AUTHORS:  - Reed EF

INSTITUCIÓN / INSTITUTION:  - UCLA Immunogenetics Center, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA. ereed@mednet.ucla.edu

RESUMEN / SUMMARY:  - MHC class I molecules have long been recognized for their ability to stimulate intracellular signals in T and B lymphocytes. More recently, it has become clear that MHC class I molecules can also initiate intracellular signals in endothelial and smooth muscle cells, which synergize with growth factor receptors to elicit cell proliferation. This review describes our current knowledge of class I-mediated signaling pathways in human endothelial and smooth muscle cells. The role of the class I signaling pathway in modulating cell growth and the clinical significance of this pathway in chronic allograft rejection are discussed.  N. Ref:: 190

 

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[179]

TÍTULO / TITLE:  - Targeting proximal T cell receptor signaling in transplantation.

REVISTA / JOURNAL:  - Transplantation 2003 Jun 27;75(12):1921-7.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000070168.42915.47

AUTORES / AUTHORS:  - Hamawy MM

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, University of Wisconsin, Madison, 53792, USA. hamawy@surgery.wisc.edu

RESUMEN / SUMMARY:  - In the past two decades, an immense amount of information has been generated on the mechanism of T cell receptor (TCR) signaling (also called signal 1). This overview describes the major signalling pathways in the TCR signal transduction cascade and focuses on proximal events in TCR signaling. The review also discusses some of the strategies that target proximal TCR signaling, which are used for preventing graft rejection.  N. Ref:: 61

 

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[180]

TÍTULO / TITLE:  - Alemtuzumab (Campath-1H) for treatment of lymphoid malignancies in the age of nonmyeloablative conditioning?

REVISTA / JOURNAL:  - Bone Marrow Transplant 2002 Dec;30(12):797-804.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.bmt.1703733

AUTORES / AUTHORS:  - Hale G; Slavin S; Goldman JM; Mackinnon S; Giralt S; Waldmann H

INSTITUCIÓN / INSTITUTION:  - Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.

RESUMEN / SUMMARY:  - The anti-CD52 (Campath-1) monoclonal antibodies (Mabs) have a substantial history of use for controlling graft-versus-host disease in allogeneic bone marrow transplantation. Now, with the availability of a humanised form, alemtuzumab (Campath-1H), and the demonstration that this agent can reduce the tumour burden in B-CLL, a new niche may be found - as a potentially curative agent in which its tumour purging ability in vivo combines with its role as a conditioning agent in nonmyeloablative transplantation. Review of the literature shows that alemtuzumab has unique advantages as a method of depleting malignant lymphocytes, including those in patients resistant to conventional chemotherapy. Alemtuzumab can also be used in BMT for depletion of normal T and B lymphocytes of both the recipient and donor for prevention of graft rejection and GVHD. It allows good stem cell recovery with resultant rapid engraftment, has a low risk of EBV-triggered secondary malignancy and does not interfere with blood stem cell mobilisation. As a method of eliminating the malignant clone in B-CLL, alemtuzumab has shown remarkable efficacy in heavily pre-treated patients, a number of whom have progressed to autologous or allogeneic transplantation. Efficacy data are shown within the context of other transplantation data for B-CLL. These results indicate that the combination of tumour-depleting and immunosuppressive properties of alemtuzumab should be explored, with the hope of providing improved treatment options for elderly patients with advanced B-CLL or indolent lymphoma whose prognosis is too poor currently to allow treatment with traditional regimens of high-dose myeloablative chemotherapy.  N. Ref:: 74

 

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[181]

TÍTULO / TITLE:  - Modulation of T cell immunity by TCR/pMHC dwell time and activating/inhibitory receptor pairs on the antigen-presenting cell.

REVISTA / JOURNAL:  - Curr Pharm Des 2003;9(3):233-44.

AUTORES / AUTHORS:  - Kalergis AM

INSTITUCIÓN / INSTITUTION:  - Department of Molecular Genetics and Immunology, The Rockefeller University. Departmento de Genetica Molecular y Microbiologia, Pontificia Universidad Catolica de Chile. kalergi@rockefeller.edu

RESUMEN / SUMMARY:  - The molecular interactions occurring at the interface between the antigen presenting cell (APC) and the T lymphocyte play an important role in the immune surveillance against infectious agents and tumors, as well as in autoimmunity and transplant rejection. The significance of the APC-T cell interaction in immunity is underscored by the observation that deficiencies in the function of either one of these two cell types cause extreme susceptibility to infections and tumor growth. Furthermore, a disregulated APC-T cell interaction can initiate autoimmunity. Thus, antigen recognition by T cells must be tightly regulated in order to ensure protection against pathogens and tumors, avoiding activation of self-reactive T cells. Efficient T cell activation requires two simultaneous signals provided by the APC: Antigen (or signal 1) and co-stimulation (or signal 2). The specificity of antigen recognition by T cells (signal 1) is controlled exclusively by the T cell receptor (TCR), an extremely diverse heterodimeric protein composed of disulfide-bonded alpha and beta chains. While it is clear that the TCR recognizes antigens as small peptides bound to molecules of the Major Histocompatibility Complex (MHC), the molecular explanation for the specificity of antigen recognition by the betaalphaTCR is just beginning to be elucidated. In this review are described some of the advances made in the understanding of the molecular interactions that define the antigen-specificity of the TCR, and the current models for T cell activation by antigen on APCs are discussed.  N. Ref:: 128

 

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[182]

TÍTULO / TITLE:  - Monoclonal antibodies for the prevention and treatment of graft-versus-host disease.

REVISTA / JOURNAL:  - Semin Oncol 2003 Aug;30(4):509-19.

AUTORES / AUTHORS:  - Bruner RJ; Farag SS

INSTITUCIÓN / INSTITUTION:  - Bone Marrow Transplantation Program, The Ohio State University Comprehensive Cancer Center, Columbus, USA.

RESUMEN / SUMMARY:  - Acute and chronic graft-versus-host disease (GvHD) remain major obstacles to successful allogeneic hematopoietic stem cell transplantation, contributing substantially to morbidity and non-relapse mortality. Better understanding of the immunopathophysiology of GvHD has identified a number of targets for intervention. Among newly developed agents suitable for the prevention and treatment of GvHD, monoclonal antibodies hold much promise. Monoclonal antibodies currently available, such as infliximab and anti-interferon-gamma (anti-IFN-gamma), are capable of blocking of the action of initiating and effector cytokines. Antibodies directed against activated T cells, including daclizumab, visilizumab and ABX-CBL, may offer more specificity than the more broadly acting pan-T-cell-depleting agents. Finally, the clinical investigation of antibodies to adhesion molecules (such as LFA-1), or distal effector mechanisms (such as FasL) may offer another level of specificity. Many of these monoclonal antibodies have already undergone clinical testing. Campath-1H has been used for the prevention of acute GvHD with success. Daclizumab, infliximab, visilizumab, and ABX-CBL have shown promising activity in steroid-resistant acute GvHD in early clinical testing. This review summarizes current experience with monoclonal antibodies in the management of acute and chronic GvHD. Over the next decade, however, the challenge will be to define the relative place of these antibodies in the therapeutic armamentarium for GvHD and their impact on long-term survival.  N. Ref:: 101

 

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[183]

TÍTULO / TITLE:  - Genetic predisposition to infectious pathogens: a review of less familiar variants.

REVISTA / JOURNAL:  - Pediatr Infect Dis J 2003 May;22(5):457-61.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.inf.0000068205.82627.55

AUTORES / AUTHORS:  - Somech R; Amariglio N; Spirer Z; Rechavi G

INSTITUCIÓN / INSTITUTION:  - Pediatric Hemato-Oncology, Chaim Sheba Medical Center, Tel Hashomer, Israel.

RESUMEN / SUMMARY:  - The susceptibility and clinical manifestations of infectious diseases in human populations are influenced by a variety of factors, among them host genetics. Obvious examples for the effect of host genetics on predisposition to unique infections are the primary immunodeficiency diseases. Minor gene variants that influence the host immune system are much more common. The iceberg model can be used to illustrate the epidemiology of immunodeficiency states. Accordingly only a few individuals have known and severe recognized primary immunodeficiencies, whereas many more patients have mild immunodeficiencies that may remain undiagnosed and are predisposed to a unique infectious disease. We review some of the less common variants that influence the host defense and predispose to certain infectious agents or change their outcome.  N. Ref:: 43

 

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[184]

TÍTULO / TITLE:  - Tissue factor and coronary artery disease.

REVISTA / JOURNAL:  - Cardiovasc Res 2002 Feb 1;53(2):313-25.

AUTORES / AUTHORS:  - Moons AH; Levi M; Peters RJ

INSTITUCIÓN / INSTITUTION:  - Department of Cardiology, Academic Medical Center, Room F3-236, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.

RESUMEN / SUMMARY:  - Plaque disruption with superimposed thrombosis is the main cause of acute coronary events such as acute myocardial infarction and unstable angina. Among other factors, tissue factor seems to play an important role determining plaque thrombogenicity. Tissue factor is a potent initiator of the coagulation cascade situated within the vessel wall and is highly exposed to the blood after plaque rupture. Several mediators involved in the process of atherosclerotic plaque formation are capable of inducing tissue factor expression in cells such as monocytes, macrophages and endothelial cells, which under normal conditions do not express tissue factor or to a limited extent only. The increased expression of tissue factor is not limited to the plaque but is also found in circulating monocytes in patients with acute coronary syndromes. In addition, studies have shown an important contribution of tissue factor in the pathogenesis of thrombosis and restenosis after balloon angioplasty. Recent basic studies focus on the therapeutic inhibition of tissue factor. Specific and non-specific inhibitors of tissue factor or the tissue factor/factor VIIa complex have been developed or identified, and have been tested in experimental studies. Clinical studies are currently being initiated. In this review, we present the current knowledge on the role of tissue factor in atherosclerosis, arterial intervention and potential pharmacological approaches, with focus on acute coronary syndromes.  N. Ref:: 162

 

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[185]

TÍTULO / TITLE:  - HFE gene and hereditary hemochromatosis: a HuGE review. Human Genome Epidemiology.

REVISTA / JOURNAL:  - Am J Epidemiol 2001 Aug 1;154(3):193-206.

AUTORES / AUTHORS:  - Hanson EH; Imperatore G; Burke W

INSTITUCIÓN / INSTITUTION:  - United States Air Force School of Aerospace Medicine, Brooks Air Force Base, San Antonio, TX, USA. erichansonmdmph@yahoo.com

RESUMEN / SUMMARY:  - Hereditary hemochromatosis (HHC) is an autosomal recessive disorder of iron metabolism characterized by increased iron absorption and deposition in the liver, pancreas, heart, joints, and pituitary gland. Without treatment, death may occur from cirrhosis, primary liver cancer, diabetes, or cardiomyopathy. In 1996, HFE, the gene for HHC, was mapped on the short arm of chromosome 6 (6p21.3). Two of the 37 allelic variants of HFE described to date (C282Y and H63D) are significantly correlated with HHC. Homozygosity for the C282Y mutation was found in 52-100% of previous studies on clinically diagnosed probands. In this review, 5% of HHC probands were found to be compound heterozygotes (C282Y/H63D), and 1.5% were homozygous for the H63D mutation; 3.6% were C282Y heterozygotes, and 5.2% were H63D heterozygotes. In 7% of cases, C282Y and H63D mutations were not present. In the general population, the frequency of the C282Y/C282Y genotype is 0.4%. C282Y heterozygosity ranges from 9.2% in Europeans to nil in Asian, Indian subcontinent, African/Middle Eastern, and Australasian populations. The H63D carrier frequency is 22% in European populations. Accurate data on the penetrance of the different HFE genotypes are not available. Extrapolating from limited clinical observations in screening studies, an estimated 40--70% of persons with the C282Y homozygous genotype will develop clinical evidence of iron overload. A smaller proportion will die from complications of iron overload. To date, population screening for HHC is not recommended because of uncertainties about optimal screening strategies, optimal care for susceptible persons, laboratory standardization, and the potential for stigmatization or discrimination.  N. Ref:: 103

 

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[186]

TÍTULO / TITLE:  - Ethical considerations on preimplantation genetic diagnosis for HLA typing to match a future child as a donor of haematopoietic stem cells to a sibling.

REVISTA / JOURNAL:  - Hum Reprod 2002 Mar;17(3):534-8.

AUTORES / AUTHORS:  - Pennings G; Schots R; Liebaers I

INSTITUCIÓN / INSTITUTION:  - Department of Philosophy, Lok. 5 C 442, Academic Hospital, Free University Brussels, Pleinlaan 2, B-1050 Brussels, Belgium. gpenning@vub.ac.be

RESUMEN / SUMMARY:  - Recently, several requests were made by couples with an affected child who wanted preimplantation genetic diagnosis (PGD) to select embryos in the hope of conceiving an HLA identical donor sibling. This article considers the ethical arguments for and against the application of PGD for this goal. Only embryos HLA matched with an existing sibling in need of a compatible donor of haematopoietic stem cells would be transferred. The main arguments are the instrumentalization of the child, the best-interests standard, the postnatal test for acceptability and the experience of the donor child. It is argued that conceiving a child to save a child is a morally defensible decision on the condition that the operation that will be performed on the future child is acceptable to perform on an existing child. The instrumentalization of the donor child does not demonstrate disrespect for its autonomy or its intrinsic worth.  N. Ref:: 29

 

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[187]

TÍTULO / TITLE:  - Host and viral genetics and risk of cervical cancer: a review.

REVISTA / JOURNAL:  - Virus Res 2002 Nov;89(2):229-40.

AUTORES / AUTHORS:  - Hildesheim A; Wang SS

INSTITUCIÓN / INSTITUTION:  - Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd, Room 7062, EPS/MSC# 7234, Rockville, MD 20852, USA. hildesha@exchange.nih.gov

RESUMEN / SUMMARY:  - Infection with human papillomaviruses (HPV) is known to play a central role in the development of cervical cancer. Both host and viral genetic factors have been postulated to be important determinants of risk of HPV progression to neoplasia among infected individuals. In this report, we review epidemiological studies that have evaluated the role in cervical cancer pathogenesis of genetic variation in human leukocyte antigen (HLA) genes and in the HPV genome itself. A protective effect of HLA Class II DRB1*13/DBQ1*0603 alleles is the most consistent HLA finding in the published literature. A consistent association between HPV16 non-European variants and risk of disease is also evident from published work. These findings are discussed. Gaps in our understanding and future research needs are also discussed.  N. Ref:: 90

 

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[188]

TÍTULO / TITLE:  - Preventing saphenous vein graft failure: does gene therapy have a role?

REVISTA / JOURNAL:  - Ann Thorac Surg 2003 Sep;76(3):959-66.

AUTORES / AUTHORS:  - Akowuah EF; Sheridan PJ; Cooper GJ; Newman C

INSTITUCIÓN / INSTITUTION:  - Cardiovascular Research Group, The University of Sheffield, Sheffield, United Kingdom. akowuah@yahoo.com

RESUMEN / SUMMARY:  - Gene therapy potentially allows local delivery and expression of cytokines, growth factors, and other mediators. In spite of increasing knowledge of the human genome, applications in clinical practice are only just beginning. The main limitations of effective clinical gene therapy are safety and low transfection efficiency. Saphenous vein grafts permit the transfection of the conduit ex vivo. This allows a variety of transfection techniques to be used, enhancing the transfection efficiency while limiting the risk of systemic complications. This review examines the potential mechanisms of gene delivery and genetic targets that may be applied to saphenous vein graft failure.  N. Ref:: 68

 

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[189]

- Castellano -

TÍTULO / TITLE:Las inmunofilinas: agentes neuroprotectores y promotores de la regeneracion neural. Immunophilins: neuroprotective agents and promoters of neural regeneration.

REVISTA / JOURNAL:  - Neurologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.stmeditores.com/Revistas/ffasciculo.php?Mw==&MTk=&MjUy 

      ●● Cita: Neurologia: <> 2002 Apr;17(4):200-13.

AUTORES / AUTHORS:  - Udina E; Navarro X

INSTITUCIÓN / INSTITUTION:  - Grupo de Neuroplasticidad y Regeneracion, Departamento de Biologia Celular, Fisiologia e Inmunologia, Universitat Autonoma de Barcelona, Bellaterra, España.

RESUMEN / SUMMARY:  - Immunophilins are a family of proteins mainly known because they act as receptors of the immunosuppressant drugs cyclosporin A (CsA) and FK506. Immunophilins serve several general functions, including regulation of mitochondrial permeability, modulation of ion channels stability and acting as chaperones for a variety of proteins. However, immunophilins are also present at high density in the nervous system. CsA, FK506 and other derivatives inhibit the function of immunophilins and, through bloking or activating several intracellular pathways, it has been shown that they exert neuroprotective effects in different experimental models of ischemia, Parkinson’s disease and excitotoxic insults. Moreover, FK506 also has neuroregenerative effects, by enhancing the axonal regeneration rate after lesions of the peripheral nervous system. The development of new agents that selectively bind to immunophilins opens new interesting perspectives for the therapy of degenerative diseases and injuries of the nervous system.  N. Ref:: 100

 

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[190]

TÍTULO / TITLE:  - Protein phosphatase 2A on track for nutrient-induced signalling in yeast.

REVISTA / JOURNAL:  - Mol Microbiol. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.blackwell-synergy.com/ 

      ●● Cita: Molecular Microbiology: <> 2002 Feb;43(4):835-42.

AUTORES / AUTHORS:  - Zabrocki P; Van Hoof C; Goris J; Thevelein JM; Winderickx J; Wera S

INSTITUCIÓN / INSTITUTION:  - Laboratorium voor Moleculaire Celbiologie, K.U.Leuven, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium.

RESUMEN / SUMMARY:  - Early studies identified two bona fide protein phosphatase 2A (PP2A)-encoding genes in Saccharomyces cerevisiae, designated PPH21 and PPH22. In addition, three PP2A-related phosphatases, encoded by PPH3, SIT4 and PPG1, have been identified. All share as much as 86% sequence similarity at the amino acid level. This review will focus primarily on Pph21 and Pph22, but some aspects of Sit4 regulation will also be discussed. Whereas a role for PP2A in yeast morphology and cell cycle has been readily recognized, uncovering its function in yeast signal transduction is a more recent breakthrough. Via their interaction with phosphorylated Tap42, PP2A and Sit4 play a pivotal role in target of rapamycin (TOR) signalling. PPH22 overexpression mimics overactive cAMP-PKA (protein kinase A) signalling and PP2A and Sit4 might represent ceramide signalling targets. The methylation of its catalytic subunit stabilizes the heterotrimeric form of PP2A and might counteract TOR signalling. We will show how these new elements could lead us to understand the role and regulation of PP2A in nutrient-induced signalling in baker’s yeast.  N. Ref:: 41

 

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[191]

TÍTULO / TITLE:  - Acremonium strictum pulmonary infection in a leukemic patient successfully treated with posaconazole after failure of amphotericin B.

REVISTA / JOURNAL:  - Eur J Clin Microbiol Infect Dis 2002 Nov;21(11):814-7. Epub 2002 Oct 31.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s10096-002-0828-8

AUTORES / AUTHORS:  - Herbrecht R; Letscher-Bru V; Fohrer C; Campos F; Natarajan-Ame S; Zamfir A; Waller J

INSTITUCIÓN / INSTITUTION:  - Departement d’Hematologie et d’Oncologie, Hopital de Hautepierre, Avenue Moliere, 67098 Strasbourg, France. raoul.herbrecht@chru-strasbourg.fr

RESUMEN / SUMMARY:  - A severely neutropenic patient with chronic lymphocytic leukemia developed a diffuse bilateral pulmonary infection while receiving a therapeutic daily dosage of intravenous amphotericin B for Candida glabrata esophagitis. Computed tomography of the chest showed numerous lung nodules, ground glass areas and a pleural effusion. Biopsy of one nodule demonstrated hyaline septate hyphae. Multiple sputum cultures grew Acremonium strictum. Increasing the dose of amphotericin B and the addition of itraconazole did not resolve the infection. Change of treatment to posaconazole given orally at 200 mg four times/d resulted in progressive improvement leading finally to cure after 24 weeks of therapy. Treatment with posaconazole was clinically and biologically well tolerated.  N. Ref:: 15

 

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[192]

TÍTULO / TITLE:  - mTOR as a target for cancer therapy.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2004;279:339-59.

AUTORES / AUTHORS:  - Houghton PJ; Huang S

INSTITUCIÓN / INSTITUTION:  - Department of Molecular Pharmacology, St. Jude Children’s Research Hospital, 332 N. Lauderdale, Memphis, TN 38105-2794, USA. peter.houghton@stjude.org

RESUMEN / SUMMARY:  - The target of rapamycin, mTOR, acts as a sensor for mitogenic stimuli, such as insulin-like growth factors and cellular nutritional status, regulating cellular growth and division. As many tumors are driven by autocrine or paracrine growth through the type-I insulin-like growth factor receptor, mTOR is potentially an attractive target for molecular-targeted treatment. Further, a rationale for anticipating tumor-selective activity based on transforming events frequently identified in malignant disease is becoming established.  N. Ref:: 73

 

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[193]

TÍTULO / TITLE:  - Hepatitis B core antibody-positive grafts: recipient’s risk.

REVISTA / JOURNAL:  - Transplantation 2003 Feb 15;75(3 Suppl):S49-53.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000047006.96782.64

AUTORES / AUTHORS:  - de Villa VH; Chen YS; Chen CL

INSTITUCIÓN / INSTITUTION:  - Liver Transplant Program, Department of Surgery, Chang Gung University, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Kaohsiung, Taiwan.

RESUMEN / SUMMARY:  - The transmission of hepatitis B virus infection through hepatitis B core antibody (anti-HBc)-positive liver grafts in hepatitis B surface antigen (HBsAg)-negative recipients has been established. The mandatory use of immunosuppression in transplant patients favors reactivation of latent virus that may be present in grafts from HBsAg-negative anti-HBc-positive donors. With the persistent organ donor scarcity, the use of these grafts cannot be avoided, especially in urgent cases and in areas where the prevalence of the hepatitis B virus is high, as in Asia. The recognition of posttransplant de novo hepatitis B from core antibody-positive liver donors has, therefore, led to modifications in graft allocation policies and the introduction of strategies for prophylaxis. The risk of developing this type of new-onset hepatitis B virus infection in liver transplant recipients and the various approaches to minimize this risk are reviewed. The peculiar implications of using core antibody-positive grafts in the context of living donor liver transplantation in Asia are discussed.  N. Ref:: 30

 

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[194]

TÍTULO / TITLE:  - Commentary. Major breakthrough in the HLA-G debate: occurrence of pregnancy in human depends on the HLA-G status of preimplantation embryos.

REVISTA / JOURNAL:  - Eur J Immunol 2002 Feb;32(2):309-10.

      ●● Enlace al texto completo (gratuito o de pago) 1002/1521-4141(200202)32:2<309::AID-IMMU309>3.0.CO;2-H [pii]

AUTORES / AUTHORS:  - Bouteiller PL

INSTITUCIÓN / INSTITUTION:  - INSERM U395, CHU Purpan, BP 3028, 31024 Toulouse Cedex 3, France. phil.lb@toulouse.inserm.fr  N. Ref:: 16

 

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[195]

TÍTULO / TITLE:  - Serine-threonine protein phosphatase inhibitors: development of potential therapeutic strategies.

REVISTA / JOURNAL:  - J Med Chem 2002 Mar 14;45(6):1151-75.

AUTORES / AUTHORS:  - McCluskey A; Sim AT; Sakoff JA

INSTITUCIÓN / INSTITUTION:  - School of Biological & Chemical Science, Medicinal Chemistry Group, The University of Newcastle, Callaghan, NSW 2308, Australia. amcclusk@mail.newcastle.edu.au  N. Ref:: 329

 

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[196]

TÍTULO / TITLE:  - Filgrastim treatment of acute myelogenous leukemia (M7) relapse after allogeneic peripheral stem cell transplantation resulting in both graft-versus-leukemia effect with cytogenetic remission and chronic graft-versus-host disease manifesting as polyserositis and subsequent bronchiolitis obliterans with organizing pneumonia.

REVISTA / JOURNAL:  - Int J Hematol 2002 Nov;76(4):360-4.

AUTORES / AUTHORS:  - Law L; Tuscano J; Wun T; Ahlberg K; Richman C

INSTITUCIÓN / INSTITUTION:  - Division of Hematology/Oncology, Department of Internal Medicine, University of California, Davis, California, USA.

RESUMEN / SUMMARY:  - Filgrastim (granulocyte colony-stimulating factor) has recently been reported to successfully treat patients with leukemic relapse after allogeneic peripheral stem cell transplantation (PSCT). However, the majority of the patients who responded also developed graft-versus-host disease (GVHD). Polyserositis as a manifestation of GVHD is a rare phenomenon. We report the first case of polyserositis following the use of filgrastim to treat a patient with acute myelogenous leukemia (M7), who had relapsed after an initially successful allogeneic PSCT. The polyserositis manifested with effusions and was initially controlled with high doses of steroids and pericardial stripping; however, after a quiescent period the patient eventually developed bronchiolitis obliterans with organizing pneumonia that required additional immunosuppressive therapy. We review the literature on GVHD-associated polyserositis and offer potential explanations for its pathogenesis.  N. Ref:: 20

 

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[197]

TÍTULO / TITLE:  - Inflammatory myopathies: clinical, diagnostic and therapeutic aspects.

REVISTA / JOURNAL:  - Muscle Nerve 2003 Apr;27(4):407-25.

      ●● Enlace al texto completo (gratuito o de pago) 1002/mus.10313

AUTORES / AUTHORS:  - Mastaglia FL; Garlepp MJ; Phillips BA; Zilko PJ

INSTITUCIÓN / INSTITUTION:  - Centre for Neuromuscular and Neurological Disorders, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Australia. flmast@cyllene.uwa.edu.au

RESUMEN / SUMMARY:  - The three major forms of immune-mediated inflammatory myopathy are dermatomyositis (DM), polymyositis (PM), and inclusion-body myositis (IBM). They each have distinctive clinical and histopathologic features that allow the clinician to reach a specific diagnosis in most cases. Magnetic resonance imaging is sometimes helpful, particularly if the diagnosis of IBM is suspected but has not been formally evaluated. Myositis-specific antibodies are not helpful diagnostically but may be of prognostic value; most antibodies have low sensitivity. Muscle biopsy is mandatory to confirm the diagnosis of an inflammatory myopathy and to allow unusual varieties such as eosinophilic, granulomatous, and parasitic myositis, and macrophagic myofasciitis, to be recognized. The treatment of the inflammatory myopathies remains largely empirical and relies upon the use of corticosteroids, immunosuppressive agents, and intravenous immunoglobulin, all of which have nonselective effects on the immune system. Further controlled clinical trials are required to evaluate the relative efficacy of the available therapeutic modalities particularly in combinations, and of newer immunosuppressive agents (mycophenolate mofetil and tacrolimus) and cytokine-based therapies for the treatment of resistant cases of DM, PM, and IBM. Improved understanding of the molecular mechanisms of muscle injury in the inflammatory myopathies should lead to the development of more specific forms of immunotherapy for these conditions.  N. Ref:: 256

 

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[198]

TÍTULO / TITLE:  - MHC class I antigen processing regulated by cytosolic proteolysis-short cuts that alter peptide generation.

REVISTA / JOURNAL:  - Mol Immunol 2002 Oct;39(3-4):171-9.

AUTORES / AUTHORS:  - Kessler BM; Glas R; Ploegh HL

INSTITUCIÓN / INSTITUTION:  - Department of Pathology, Harvard Medical School, Room 137, Building D2, 200 Longwood Avenue, Boston, MA 02115, USA.

RESUMEN / SUMMARY:  - Cytotoxic T lymphocyte (CTL)-mediated immune responses rely on the efficiency of MHC class I ligand generation and presentation by antigen presenting cells (APCs). Whereas the abnormal expression of MHC molecules and transporters associated with antigen processing (TAPs) are commonly discussed as factors that modulate antigen presentation, much less is known about possible regulatory mechanisms at the level of proteolysis responsible for the generation of antigenic peptides. The ubiquitin-proteasome system is recognized as the major component responsible for this process in the cytosol and its activity can be regulated by cytokines, such as IFN-gamma. However, new evidence suggests the involvement of other proteases that can contribute to cytosolic proteolysis and therefore, to the quality and quantity of antigen production. Here, we review recent findings on an increasing number of proteolytic enzymes linked to antigen presentation, and we discuss how regulation of cytosolic protease activities might have implications for immune escape mechanisms that could be used by tumor cells and pathogens.  N. Ref:: 99

 

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[199]

TÍTULO / TITLE:  - MHC class I receptors on natural killer cells: on with the old and in with the new.

REVISTA / JOURNAL:  - Clin Sci (Lond) 2003 Aug;105(2):127-40.

      ●● Enlace al texto completo (gratuito o de pago) 1042/CS20030095

AUTORES / AUTHORS:  - Khakoo SI; Brooks CR

INSTITUCIÓN / INSTITUTION:  - Division of Infection Inflammation and Repair, School of Medicine, University of Southampton, Mailpoint 811, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK. sik@soton.ac.uk

RESUMEN / SUMMARY:  - The functions of natural killer (NK) cells are controlled by an abundance of activating and inhibitory receptors. Many of these interact with MHC class I molecules. The MHC system also interacts with cytotoxic T-lymphocytes and has been shown to comprise a rapidly evolving family of molecules. This challenges the functional relationship of NK cell receptors with their ligands. Although individual receptors have become subject to species-specific expansions over evolutionary time, the main themes of the NK cell interaction with MHC class I have been preserved. This review details the interaction of NK cell receptors with MHC class I and discusses their unexpectedly rapid evolution.  N. Ref:: 122

 

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[200]

TÍTULO / TITLE:  - Mitochondrial permeability transition in acute neurodegeneration.

REVISTA / JOURNAL:  - Biochimie 2002 Feb-Mar;84(2-3):241-50.

AUTORES / AUTHORS:  - Friberg H; Wieloch T

INSTITUCIÓN / INSTITUTION:  - Laboratory for Experimental Brain Research, Wallenberg Neuroscience Center, BMC A13, 221 84 Lund, Sweden.

RESUMEN / SUMMARY:  - Acute neurodegeneration in man is encountered during and following stroke, transient cardiac arrest, brain trauma, insulin-induced hypoglycemia and status epilepticus. All these severe clinical conditions are characterized by neuronal calcium overload, aberrant cell signaling, generation of free radicals and elevation of cellular free fatty acids, conditions that favor activation of the mitochondrial permeability transition pore (mtPTP). Cyclosporin A (CsA) and its analog N-methyl-valine-4-cyclosporin A (MeValCsA) are potent blockers of the mtPTP and protect against neuronal death following excitotoxicity and oxygen glucose deprivation. Also, CsA and MeValCsA diminish cell death following cerebral ischemia, trauma, and hypoglycemia. Here we present data that strongly imply the mtPT in acute neurodegeneration in vivo. Compounds that readily pass the blood-brain-barrier (BBB) and block the mtPT may be neuroprotective in stroke.  N. Ref:: 100

 

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