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Revisiones-Clínica-Etiología & Patología *** Reviews-Clinical-Etiology & Pathology

 

AGENTES INMUNOSUPRESORES *** IMMUNOSUPPRESSIVE COMPOUNDS

(Conceptos / Keywords: Immunosuppressive comp; Muromonab-cd3; Sirolimus; Tacrolimus; Cyclosporine; Mycophenolic acid; Antilymphocyte serum; Immunosuppressive comp. used in oncology, etc).

 

Enero / January 2001 --- Marzo / March 2004

 

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Enlace / Link

 

[1]

TÍTULO / TITLE:  - Integration of growth factor and nutrient signaling: implications for cancer biology.

REVISTA / JOURNAL:  - Mol Cell 2003 Aug;12(2):271-80.

AUTORES / AUTHORS:  - Shamji AF; Nghiem P; Schreiber SL

INSTITUCIÓN / INSTITUTION:  - Harvard Biophysics Program, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.

RESUMEN / SUMMARY:  - Signaling networks that promote cell growth are frequently dysregulated in cancer. One regulatory network, which converges on effectors such as 4EBP1 and S6K1, leads to growth by promoting protein synthesis. Here, we discuss how this network is regulated by both extracellular signals, such as growth factors, and intracellular signals, such as nutrients. We discuss how mutations amplifying either type of signal can lead to tumor formation. In particular, we focus on the recent discovery that a tumor suppressor complex whose function is lost in tuberous sclerosis patients regulates the nutrient signal carried by the critical signaling protein TOR to the effectors 4EBP1 and S6K1. Finally, we describe how the small molecule rapamycin, which inhibits TOR and thereby the activation of these effectors, could be useful to treat tumors that have become dependent upon this pathway for growth.  N. Ref:: 80

 

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[2]

TÍTULO / TITLE:  - Dendritic cells: emerging pharmacological targets of immunosuppressive drugs.

REVISTA / JOURNAL:  - Nat Rev Immunol 2004 Jan;4(1):24-34.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri1256

AUTORES / AUTHORS:  - Hackstein H; Thomson AW

INSTITUCIÓN / INSTITUTION:  - Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig University Giessen, Langhansstr. 7, D-35392 Giessen, Germany. holger.hackstein@immunologie.med.uni-giessen.de

RESUMEN / SUMMARY:  - Immunosuppressive drugs have revolutionized organ transplantation and improved the therapeutic management of autoimmune diseases. The development of immunosuppressive drugs and understanding of their action traditionally has been focused on lymphocytes, but recent evidence indicates that these agents interfere with immune responses at the earliest stage, targeting key functions of dendritic cells (DCs). Here, we review our present understanding of how classical and new immunosuppressive agents interfere with DC development and function. This knowledge might provide a rational basis for the selection of immunosuppressive drugs in different clinical settings and for the generation of tolerogenic DCs in the laboratory.  N. Ref:: 116

 

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[3]

- Castellano -

TÍTULO / TITLE:La ruta de senalizacion CA++/calcineurina/NFAT en activacion endotelial y angiogenesis: efectos de la ciclosporina A. CA++/ calcineurin/NFAT signaling in endothelial activation and angiogenesis: effects od cyclosporin A .

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2003;23 Suppl 3:44-8.

AUTORES / AUTHORS:  - Quesada AJ; Redondo JM

INSTITUCIÓN / INSTITUTION:  - Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid y Centro Nacional de Investigaciones Cardiovasculares (CNIC), Sinesio Delgado, 4 28049 Cantoblanco, Madrid. jmredondo@cbm.uam.es  N. Ref:: 31

 

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[4]

TÍTULO / TITLE:  - Interleukin-2 receptor monoclonal antibodies in renal transplantation: meta-analysis of randomised trials.

REVISTA / JOURNAL:  - British Medical J (BMJ). Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://bmj.com/search.dtl 

      ●● Cita: British Medical J. (BMJ): <> 2003 Apr 12;326(7393):789.

      ●● Enlace al texto completo (gratuito o de pago) 1136/bmj.326.7393.789

AUTORES / AUTHORS:  - Adu D; Cockwell P; Ives NJ; Shaw J; Wheatley K

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Queen Elizabeth Hospital, Birmingham, B15 2TH. dwomoa.adu@uhb.nhs.uk

RESUMEN / SUMMARY:  - OBJECTIVE: To study the effect of interleukin-2 receptor monoclonal antibodies on acute rejection episodes, graft loss, deaths, and rate of infection and malignancy in patients with renal transplants. DESIGN: Meta-analysis of published data. DATA SOURCES: Medline, Embase, and Cochrane library for years 1996-2003 plus search of medical editors’ trial amnesty and contact with manufacturers of the antibodies. SELECTION OF STUDIES: Randomised controlled trials comparing interleukin-2 receptor antibodies with placebo or no additional treatment in patients with renal transplants receiving ciclosporin based immunosuppression. RESULTS: Eight randomised controlled trials involving 1871 patients met the selection criteria (although only 1858 patients were analysed). Interleukin-2 receptor antibodies significantly reduced the risk of acute rejection (odds ratio 0.51, 95% confidence interval 0.42 to 0.63). There were no significant differences in the rate of graft loss (0.78, 0.58 to 1.04), mortality (0.75, 0.46 to 1.23), overall incidence of infections (0.97, 0.77 to 1.24), incidence of cytomegalovirus infections (0.81, 0.62 to 1.04), or risk of malignancies at one year (0.82, 0.39 to 1.70). The different antibodies had a similar sized effect on acute rejection (test for heterogeneity P=0.7): anti-Tac (0.37, 0.16 to 0.89), BT563 (0.37, 0.1 to 1.38), basiliximab (0.56, 0.44 to 0.72), and daclizumab (0.46, 0.32 to 0.67). The reduction in acute rejections was similar for all ciclosporin based immunosuppression regimens (test for heterogeneity P=1.0). CONCLUSIONS: Adding interleukin-2 receptor antibodies to ciclosporin based immunosuppression reduces episodes of acute rejection at six months by 49%. There is no evidence of an increased risk of infective complications. Longer follow up studies are needed to confirm whether interleukin-2 receptor antibodies improve long term graft and patient survival.

 

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[5]

TÍTULO / TITLE:  - Novel therapeutic molecular targets for prostate cancer: the mTOR signaling pathway and epidermal growth factor receptor.

REVISTA / JOURNAL:  - J Urol 2004 Feb;171(2 Pt 2):S41-3; discussion S44.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.ju.0000108100.53239.b7

AUTORES / AUTHORS:  - Tolcher AW

INSTITUCIÓN / INSTITUTION:  - Director Clinical Research, Institute for Drug Development Cancer Therapy and Research Center, San Antonio, Texas, USA.

RESUMEN / SUMMARY:  - PURPOSE: The scientific rationale and existing evidence for the use of novel molecular targets in the chemoprevention of cancer are reviewed, with special attention to prostate cancer. MATERIALS AND METHODS: A search for relevant literature on basic science and clinical trials was conducted using PubMed/MEDLINE. RESULTS: The emergence of molecularly targeted therapies for advanced malignancies creates an important opportunity to examine these agents for the chemoprevention of prostate cancer. Two critical targets in the proliferation and malignant transformation of normal cells, the PI3/Akt signal transduction pathway and the epidermal growth factor receptor, are currently the focus of several novel investigational therapies that are in late stage phase II and phase III studies. CONCLUSIONS: Research to date supports consideration of these novel molecular targets as future agents in the chemoprevention of prostate cancer.  N. Ref:: 28

 

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[6]

TÍTULO / TITLE:  - A randomized long-term trial of tacrolimus/sirolimus versus tacrolimus/mycophenolate mofetil versus cyclosporine (NEORAL)/sirolimus in renal transplantation. II. Survival, function, and protocol compliance at 1 year.

REVISTA / JOURNAL:  - Transplantation 2004 Jan 27;77(2):252-8.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000101495.22734.07

AUTORES / AUTHORS:  - Ciancio G; Burke GW; Gaynor JJ; Mattiazzi A; Roth D; Kupin W; Nicolas M; Ruiz P; Rosen A; Miller J

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Division of Transplantation, University of Miami School of Medicine, Miami, FL 33101, USA. gciancio@med.miami.edu

RESUMEN / SUMMARY:  - BACKGROUND: In an attempt to reduce chronic calcineurin inhibitor induced allograft nephropathy in first cadaver and human leukocyte antigen non-identical living-donor renal transplantation, sirolimus (Siro) or mycophenolate mofetil (MMF) was tested as adjunctive therapy, with planned dose reductions of tacrolimus (Tacro) over the first year postoperatively. Adjunctive Siro therapy with a similar dose reduction algorithm for Neoral (Neo) was included for comparison. METHODS: The detailed dose reduction plan (Tacro and Siro, group A; Tacro and MMF, group B; Neo and Siro, group C) is described in our companion report in this issue of Transplantation. The present report documents function, patient and graft survival, protocol compliance, and adverse events. RESULTS: As mentioned (in companion report), group demographics were similar. The present study shows no significant differences in 1-year patient and graft survival but does show a trend that points to more difficulties in group C by way of a rising slope of serum creatinine concentration (P=0.02) and decreasing creatinine clearance (P=0.04). There were more patients who discontinued the protocol plan in group C. Thus far, no posttransplant lymphomas have appeared, and infectious complications have not differed among the groups. However, a greater percentage of patients in group C were placed on antihyperlipidemia therapy, with an (unexpected) trend toward a higher incidence of posttransplant diabetes mellitus in this group. Group A required fewer, and group B the fewest, antihyperlipidemia therapeutic interventions (P<0.00001). CONCLUSIONS: This 1-year interim analysis of a long-term, prospective, randomized renal-transplant study indicates that decreasing maintenance dosage of Tacro with adjunctive Siro or MMF appears to point to improved long-term function, with reasonably few adverse events.

 

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[7]

TÍTULO / TITLE:  - The target of rapamycin (TOR) proteins.

REVISTA / JOURNAL:  - Proc Natl Acad Sci U S A. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.pnas.org/ 

      ●● Cita: Proc Natl Acad Sci USA (PNAS): <> 2001 Jun 19;98(13):7037-44.

      ●● Enlace al texto completo (gratuito o de pago) 1073/pnas.121145898

AUTORES / AUTHORS:  - Raught B; Gingras AC; Sonenberg N

INSTITUCIÓN / INSTITUTION:  - Department of Biochemistry and McGill Cancer Centre, McGill University, 3655 Promenade Sir-William-Osler, Montreal, QC H3G 1Y6 Canada.

RESUMEN / SUMMARY:  - Rapamycin potently inhibits downstream signaling from the target of rapamycin (TOR) proteins. These evolutionarily conserved protein kinases coordinate the balance between protein synthesis and protein degradation in response to nutrient quality and quantity. The TOR proteins regulate (i) the initiation and elongation phases of translation, (ii) ribosome biosynthesis, (iii) amino acid import, (iv) the transcription of numerous enzymes involved in multiple metabolic pathways, and (v) autophagy. Intriguingly, recent studies have also suggested that TOR signaling plays a critical role in brain development, learning, and memory formation.  N. Ref:: 132

 

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[8]

TÍTULO / TITLE:  - Rapamycin plays a new role as differentiator of vascular smooth muscle phenotype. focus on “The mTOR/p70 S6K1 pathway regulates vascular smooth muscle differentiation”.

REVISTA / JOURNAL:  - Am J Physiol Cell Physiol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://ajpcell.physiology.org/contents-by-date.0.shtml 

      ●● Cita: Am J Physiol Cell Physiol: <> 2004 Mar;286(3):C480-1.

      ●● Enlace al texto completo (gratuito o de pago) 1152/ajpcell.00526.2003

AUTORES / AUTHORS:  - Lucchesi PA  N. Ref:: 12

 

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[9]

TÍTULO / TITLE:  - CD3-specific antibody-induced active tolerance: from bench to bedside.

REVISTA / JOURNAL:  - Nat Rev Immunol 2003 Feb;3(2):123-32.

      ●● Enlace al texto completo (gratuito o de pago) 1038/nri1000

AUTORES / AUTHORS:  - Chatenoud L

INSTITUCIÓN / INSTITUTION:  - Centre de l’Association Claude Bernard sur les Maladies Autoimmunes and Hopital Necker Enfants Malades IRNEM, 161 Rue de Sevres, 75015 Paris, France. chatenoud@necker.fr

RESUMEN / SUMMARY:  - Although they were used initially as non-specific immunosuppressants in transplantation, CD3-specific monoclonal antibodies have elicited renewed interest owing to their capacity to induce immune tolerance. In mouse models of autoimmune diabetes, CD3-specific antibodies induce stable disease remission by restoring tolerance to pancreatic beta-cells. This phenomenon was extended recently to the clinic—preservation of beta-cell function in recently diagnosed patients with diabetes was achieved by short-term administration of a CD3-specific antibody. CD3-specific antibodies arrest ongoing disease by rapidly clearing pathogenic T cells from the target. Subsequently, they promote long-term T-cell-mediated active tolerance. Recent data indicate that transforming growth factor-beta-dependent CD4+CD25+ regulatory T cells might have a central role in this effect.  N. Ref:: 117

 

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[10]

TÍTULO / TITLE:  - Neuroimmunophilins: novel neuroprotective and neuroregenerative targets.

REVISTA / JOURNAL:  - Ann Neurol 2001 Jul;50(1):6-16.

AUTORES / AUTHORS:  - Guo X; Dillman JF 3rd; Dawson VL; Dawson TM

INSTITUCIÓN / INSTITUTION:  - Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

RESUMEN / SUMMARY:  - Cyclosporin A (CsA) and FK506 (tacrolimus) are immunosuppresants that are widely used in organ transplantation. CsA is an 11-member cyclic peptide, whereas FK506 is a macrolide antibiotic. Recently, these powerful and useful compounds have become of great interest to neuroscientists for their unique neuroprotective and neuroregenerative effects. These drugs and nonimmunosuppressive analogs protect neurons from the effects of glutamate excitotoxicity, focal ischemia, and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic cell death. They also stimulate functional recovery of neurons in a variety of neurologic injury paradigms. These drugs exert their effects via immunophilins, the protein receptors for these agents. The immunophilin ligands show particular promise as a novel class of neuroprotective and neuroregenerative agents that have the potential to treat a variety of neurologic disorders.  N. Ref:: 102

 

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[11]

TÍTULO / TITLE:  - Preliminary guidelines for diagnosing and treating tuberculosis in patients with rheumatoid arthritis in immunosuppressive trials or being treated with biological agents.

REVISTA / JOURNAL:  - Ann Rheum Dis 2002 Nov;61 Suppl 2:ii62-3.

AUTORES / AUTHORS:  - Furst DE; Cush J; Kaufmann S; Siegel J; Kurth R

INSTITUCIÓN / INSTITUTION:  - UCLA Medical School, Los Angeles, USA Presbyterian Hospital, Dallas, USA.

 

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[12]

TÍTULO / TITLE:  - New agents in acute myeloid leukemia and other myeloid disorders.

REVISTA / JOURNAL:  - Cancer 2004 Feb 1;100(3):441-54.

      ●● Enlace al texto completo (gratuito o de pago) 1002/cncr.11935

AUTORES / AUTHORS:  - Ravandi F; Kantarjian H; Giles F; Cortes J

INSTITUCIÓN / INSTITUTION:  - Department of Leukemia, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA. fravandi@mdanderson.org

RESUMEN / SUMMARY:  - Over the past several decades, improvements in chemotherapeutic agents and supportive care have resulted in significant progress in treating patients with acute myeloid leukemia (AML). More recently, advances in understanding the biology of AML have resulted in the identification of new therapeutic targets. The success of all-trans-retinoic acid in acute promyelocytic leukemia and of imatinib mesylate in chronic myeloid leukemia have demonstrated that targeted therapy may be more effective and less toxic when well defined targets are available. At the same time, understanding mechanisms of drug resistance and means to overcome them has led to modification of some of the existing cytotoxic agents. Rational design and conduct of clinical trials is necessary to ensure that the full potential of these new agents is realized.  N. Ref:: 140

 

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[13]

TÍTULO / TITLE:  - The transplantation of hematopoietic stem cells after non-myeloablative conditioning: a cellular therapeutic approach to hematologic and genetic diseases.

REVISTA / JOURNAL:  - Immunol Res 2003;28(1):13-24.

AUTORES / AUTHORS:  - Maris M; Storb R

INSTITUCIÓN / INSTITUTION:  - Fred Hutchinson Cancer Research Center, and University of Washington, Seattle, WA, USA. mmaris@fhcrc.org

RESUMEN / SUMMARY:  - Originally, allogeneic hematopoietic stem cell transplantation (HSCT) was viewed as a form of rescue from the marrow lethal effects of high doses of chemo-radiotherapy used to both eradicate malignancy and to provide sufficient immunosuppression to ensure allogeneic engraftment. Clear evidence of a therapeutic graft-versus-tumor (GVT) effect mediated by allogeneic effector cells (T cells) has prompted the exploration of HSCT regimens that rely solely upon host immunosuppression (non-myeloablative) to facilitate allogeneic donor engraftment. The engrafted donor effector cells are then used to accomplish the task of eradicating host malignant cells. The non-myeloblative regimen developed in Seattle uses 2 Gy total body irradiation (TBI) before transplant followed by postgrafting cyclosporine (CSP) and mycophenolate mofetil (MMF). This regimen resulted in initial mixed donor-host chimerism in all patients with hematologic malignancies and genetic disorders who received HLA-matched sibling allografts. The 17% incidence of graft rejection was reduced to 3% with the addition of fludarabine, 30 mg/m2/day on d -4, -3, and -2. The non-myeloablative combination of fludarabine/TBI has also been successful at achieving high engraftment rates in recipients of 10 of 10 HLA antigen matched unrelated donor HSCTs in patients with hematologic malignancies. By reducing acute toxicities relative to conventional HSCT, most patients have received their pre- and post-HSCT therapy almost exclusively as outpatients. Acute and chronic GVHD occur after non-myeloablative HSCT, but the incidence and severity appear less compared to conventional HSCT. As in conventional transplants, immune dysregulation from GVHD and its treatment and delayed reconstitution of immune function continue to present risks to patients who have otherwise undergone successful non-myeloablative HSCT. Cellular therapeutic effects have been observed after non-myeloablative HSCT such as correction of inherited genetic disorders, and eradication of hematologic malignant diseases and renal cell carcinoma via GVT responses.  N. Ref:: 52

 

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[14]

TÍTULO / TITLE:  - Drug immunosuppression therapy for adult heart transplantation. Part 2: clinical applications and results.

REVISTA / JOURNAL:  - Ann Thorac Surg 2004 Jan;77(1):363-71.

AUTORES / AUTHORS:  - Mueller XM

INSTITUCIÓN / INSTITUTION:  - Department of Cardiovascular Surgery, Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Quebec, Canada. xavier.mueller@usherbrooke.ca

RESUMEN / SUMMARY:  - This review describes the clinical application of classical immunosuppressive drugs as well as that of more recent drugs. All current immunosuppressive drugs target T-cell activation, and cytokine production and clonal expansion, or both. Immunosuppressive protocols can be broadly divided into induction therapy, maintenance immunosuppression, and treatment of acute rejection episodes.  N. Ref:: 82

 

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[15]

TÍTULO / TITLE:  - Treatment of idiopathic nephrosis by immunophillin modulation.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2003 Aug;18 Suppl 6:vi79-86.

AUTORES / AUTHORS:  - Meyrier A

INSTITUCIÓN / INSTITUTION:  - Service de Nephrologie, Hopital Europeen Georges Pompidou, 20 rue Leblanc, F-75015 Paris, France. alain.meyrier@brs.ap-hop-paris.fr

RESUMEN / SUMMARY:  - Until 1985, glucocorticoids and cytotoxic drugs were the only treatments available for idiopathic nephrotic syndrome (nephrosis), that is, minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS). Trials of cyclosporine (CsA) treatment of nephrosis, the rationale of which was based on pathophysiologic considerations, have shown that this immunophillin modulator is effective in inducing and maintaining remission in patients suffering from idiopathic nephrotic syndrome. It appears that the best results, in the order of 80% remission rate, are obtained in steroid-sensitive cases, essentially MCD, and that in steroid-resistant FSGS the drug obtains remission in no more than 20% of the cases. Addition of glucocorticoids increases the success rate to approximately 30% of cases. Renal toxicity is proportional to previous impairment of renal function, primary renal disease (FSGS vs MCD) dosage >5.5 mg/kg/day and duration of treatment. The better bioavailability of the new formulation of CsA (Neoral), implies that the former dosage recommendations be reconsidered for distinctly lower figures. Repeat renal biopsy after 1 year of continuous CsA treatment is advisable, as stable serum creatinine levels may be falsely reassuring. CsA dependency is the rule during the first year of treatment. However, in some 25% of cases stable remission may be maintained after slow tapering off following 3-4 years of treatment. Other immunophillin modulators have been tried in the treatment of idiopathic nephrotic syndrome. Despite few preliminary reports indicating some success of tacrolimus the effects of this drug do not seem convincingly superior to CsA in terms of remission rate, toxicity and dependency. Rapamycin has not been tried in the treatment of nephrosis. Anecdotal cases of de novo FSGS induced by rapamycin in transplanted patients might indicate that this drug is in fact contraindicated in the treatment of nephrosis.  N. Ref:: 36

 

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[16]

TÍTULO / TITLE:  - In vitro generation of IL-10-producing regulatory CD4+ T cells is induced by immunosuppressive drugs and inhibited by Th1- and Th2-inducing cytokines.

REVISTA / JOURNAL:  - Immunol Lett 2003 Jan 22;85(2):135-9.

AUTORES / AUTHORS:  - O’Garra A; Barrat FJ

INSTITUCIÓN / INSTITUTION:  - Division of Immunoregulation, The National Institute for Medical Research (NIMR), The Ridgeway, Mill Hill, NW7 1AA, London, UK.  N. Ref:: 40

 

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[17]

TÍTULO / TITLE:  - Risk for myopathy with statin therapy in high-risk patients.

REVISTA / JOURNAL:  - Arch Intern Med 2003 Mar 10;163(5):553-64.

AUTORES / AUTHORS:  - Ballantyne CM; Corsini A; Davidson MH; Holdaas H; Jacobson TA; Leitersdorf E; Marz W; Reckless JP; Stein EA

INSTITUCIÓN / INSTITUTION:  - Center for Cardiovascular Disease Prevention, Baylor College of Medicine, 6565 Fannin, Mail Station A601, Suite A656, Houston, TX 77030, USA. cmb@bcm.tmc.edu

RESUMEN / SUMMARY:  - Emerging data suggest that the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) offer important benefits for the large population of individuals at high risk for coronary heart disease. This population encompasses a sizable portion of individuals who are also at high risk for drug-drug interactions due to their need for multiple medications. In general, statins are associated with a very small risk for myopathy (which may progress to fatal or nonfatal rhabdomyolysis); however, the potential for drug-drug interactions is known to increase this risk in specific high-risk groups. The incidence of myopathy associated with statin therapy is dose related and is increased when statins are used in combination with agents that share common metabolic pathways. Of particular concern is the potential for interactions with other lipid-lowering agents such as fibrates and niacin (nicotinic acid), which may be used in patients with mixed lipidemia, and with immunosuppressive agents, such as cyclosporine, which are commonly used in patients after transplantation. Clinicians should be alert to the potential for drug-drug interactions to minimize the risk of myopathy during long-term statin therapy in patients at high risk for coronary heart disease.  N. Ref:: 128

 

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[18]

TÍTULO / TITLE:  - Renal transplantation: can we reduce calcineurin inhibitor/stop steroids? Evidence based on protocol biopsy findings.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2003 Mar;14(3):755-66.

AUTORES / AUTHORS:  - Gotti E; Perico N; Perna A; Gaspari F; Cattaneo D; Caruso R; Ferrari S; Stucchi N; Marchetti G; Abbate M; Remuzzi G

INSTITUCIÓN / INSTITUTION:  - Department of Medicine and Transplantation, Ospedali Riuniti di Bergamo, Mario Negri Institute for Pharmacological Research, Italy.

RESUMEN / SUMMARY:  - How to combine antirejection drugs and which is the optimal dose of steroids and calcineurin inhibitors beyond the first year after kidney transplantation to maintain adequate immunosuppression without major side effects are far from clear. Kidney transplant patients on steroid, cyclosporine (CsA), and azathioprine were randomized to per-protocol biopsy (n = 30) or no-biopsy (n = 29) 1 to 2 yr posttransplant. Steroid or CsA were discontinued or reduced on the basis of biopsy to establish effects on drug-related complications, acute rejection, and graft function over 3 yr of follow-up. Serum creatinine, GFR (plasma clearance of iohexol), RPF (renal clearance of p-aminohippurate), CsA pharmacokinetics, and adverse events were monitored yearly. At the end, patients underwent a second biopsy. Per-protocol biopsy histology revealed no lesions (n = 5, steroid withdrawal), CsA nephropathy (n = 13, CsA discontinuation/reduction), or chronic rejection (n = 12, standard therapy). Reducing the drug regimen led to overall fewer side effects related to immunosuppression as compared with standard therapy or no-biopsy. Steroids were safely stopped with no acute rejection or graft loss. Complete CsA discontinuation was associated with acute rejection in the first four patients. Lowering CsA to low target CsA trough (30 to 70 ng/ml) never led to acute rejection or major renal function deterioration. Biopsy patients on conventional regimen had no acute rejection, one graft loss, no significant change in GFR, and significant RPF decline. No-biopsy controls: no acute rejection, one graft loss, significant decline of GFR and RPF. By serial biopsy analysis, severe lesions did not develop in patients with steroid discontinuation in contrast to patients on standard therapy over follow-up. CsA reduction did not adversely affect histology. Per-protocol biopsy more than 1 yr after kidney transplantation is a safe procedure to guide change of drug regimen and to lower the risk of major side effects.

 

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[19]

TÍTULO / TITLE:  - Drug immunosuppression therapy for adult heart transplantation. Part 1: immune response to allograft and mechanism of action of immunosuppressants.

REVISTA / JOURNAL:  - Ann Thorac Surg 2004 Jan;77(1):354-62.

AUTORES / AUTHORS:  - Mueller XM

INSTITUCIÓN / INSTITUTION:  - Department of Cardiovascular Surgery, Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Quebec, Canada. xavier.mueller@usherbrooke.ca

RESUMEN / SUMMARY:  - In the early days of transplantation, immunosuppression therapy was rather broad and nonspecific, mainly using high-dose corticosteroids and azathioprine. Thereafter we progressively narrowed the target of immunosuppressive strategy starting with polyclonal antibodies. The introduction of cyclosporine, OKT3, and tacrolimus further narrowed the target on the T-cell pathways. More recently mycophenolate mofetil progressively took the place of azathioprine with its higher lymphocyte specificity and sirolimus and interleukin-2 receptor antibodies were introduced. In this field in constant movement the aim is to find a drug or a regimen that provides optimal immunosuppression therapy with minimal side effects, in other words to find the right balance between overimmunosuppression and underimmunosuppression therapy. This review is divided into two parts. The first part will provide a basic understanding of the immunologic response to allograft and explain how conventional and recently introduced immunosuppressive agents work. The second part will describe the clinical application of immunosuppressive drugs to provide practical information for those in charge of heart transplant recipients.  N. Ref:: 68

 

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[20]

TÍTULO / TITLE:  - Longstanding obliterative panarteritis in Kawasaki disease: lack of cyclosporin A effect.

REVISTA / JOURNAL:  - Pediatrics 2003 Oct;112(4):986-92.

AUTORES / AUTHORS:  - Kuijpers TW; Biezeveld M; Achterhuis A; Kuipers I; Lam J; Hack CE; Becker AE; van der Wal AC

INSTITUCIÓN / INSTITUTION:  - Emma Children’s Hospital, Academic Medical Center, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands. t.w.kuijpers@amc.uva.nl

RESUMEN / SUMMARY:  - Kawasaki disease is a childhood vasculitis of medium-sized vessels, affecting the coronary arteries in particular. We have treated a therapy-resistant child who met all diagnostic criteria for Kawasaki disease. After the boy was given intravenous immunoglobulins and salicylates, as well as several courses of pulsed methylprednisolone, disease recurred and coronary artery lesions became progressively detectable. Cyclosporin A was started and seemed clinically effective. In contrast to the positive effect on inflammatory parameters, ie, C-reactive protein and white blood cell counts, a novel plasma marker for cytotoxicity (granzyme B) remained elevated. Coronary disease progressed to fatal obstruction and myocardial infarction. Echocardiography, electrocardiograms, and myocardial creatine phosphokinase did not predict impending death. At autopsy an obliterative panarteritis was observed resulting from massive fibrointimal proliferation, affecting the aorta and several large and medium-sized arteries. Immunophenotypic analysis of the inflammatory infiltrates in arteries revealed mainly granzyme-positive cytotoxic T cells and macrophages in the intima and media, as well as nodular aggregates of T cells, B cells, and plasma cells in the adventitia of affected arteries. These findings further endorse the role of specific cellular and humoral immunity in Kawasaki disease. Unremitting coronary arteritis and excessive smooth muscle hyperplasia resulted in coronary occlusion despite the use of cyclosporin A.  N. Ref:: 37

 

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[21]

TÍTULO / TITLE:  - Mammalian target of rapamycin inhibition as therapy for hematologic malignancies.

REVISTA / JOURNAL:  - Cancer 2004 Feb 15;100(4):657-66.

      ●● Enlace al texto completo (gratuito o de pago) 1002/cncr.20026

AUTORES / AUTHORS:  - Panwalkar A; Verstovsek S; Giles FJ

INSTITUCIÓN / INSTITUTION:  - Section of Developmental Therapeutics, Department of Leukemia, University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA.

RESUMEN / SUMMARY:  - The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway, which mediates cell survival and proliferation. mTOR regulates essential signal-transduction pathways, is involved in the coupling of growth stimuli with cell cycle progression, and initiates mRNA translation in response to favorable nutrient environments. mTOR is involved in regulating many aspects of cell growth, including membrane traffic, protein degradation, protein kinase C signaling, ribosome biogenesis, and transcription. Because mTOR activates both the 40S ribosomal protein S6 kinase (p70s6k) and the eukaryotic initiation factor 4E-binding protein 1, its inhibitors cause G1-phase cell cycle arrest. Inhibitors of mTOR also prevent cyclin dependent kinase (CDK) activation, inhibit retinoblastoma protein phosphorylation, and accelerate the turnover of cyclin D1, leading to a deficiency of active CDK4/cyclin D1 complexes, all of which may help cause G1-phase arrest. It is known that the phosphatase and tensin homologue tumor suppressor gene (PTEN) plays a major role in embryonic development, cell migration, and apoptosis. Malignancies with PTEN mutations, which are associated with constitutive activation of the PI3K/Akt pathway, are relatively resistant to apoptosis and may be particularly sensitive to mTOR inhibitors. Rapamycin analogs with relatively favorable pharmaceutical properties, including CCI-779, RAD001, and AP23573, are under investigation in patients with hematologic malignancies.  N. Ref:: 116

 

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[22]

TÍTULO / TITLE:  - Cholesteryl ester transfer protein facilitates the movement of water-insoluble drugs between lipoproteins: a novel biological function for a well-characterized lipid transfer protein.

REVISTA / JOURNAL:  - Biochem Pharmacol 2002 Dec 15;64(12):1669-75.

AUTORES / AUTHORS:  - Kwong M; Wasan KM

INSTITUCIÓN / INSTITUTION:  - Division of Pharmaceutics and Biopharmaceutics, Faculty of Pharmaceutical Sciences, The University of British Columbia, 2146 East Mall Avenue, Vancouver, BC, Canada V6T 1Z3.

RESUMEN / SUMMARY:  - This review article addresses the recently discovered finding that cholesteryl ester transfer protein (CETP) can facilitate the transfer of water-insoluble drugs between different lipoprotein subclasses. This protein, which is often referred to as lipid transfer protein I (LTP I), is involved in the lipid regulation of lipoproteins. It is responsible for the facilitated transfer of core lipoprotein lipids, cholesteryl ester and triglycerides, and approximately one-third of the coat lipoprotein lipid, phosphatidylcholine, between different plasma lipoproteins. The human body appears to recognize exogenous water-insoluble drugs as lipid-like particles, which suggests that these compounds may interact with lipoproteins just like endogenous plasma lipids, and thus their transfer between lipoproteins may be facilitated by plasma CETP. Patients with a variety of diseases (i.e. diabetes, cancer, AIDS) often exhibit hypo- and/or hypercholesterolemia and triglyceridemia, commonly referred to as dyslipidemias, which result in changes in their plasma lipoprotein-lipid composition and concentration. The interaction of water-insoluble drugs with these dyslipidemic lipoproteins may be responsible for the differences seen in the pharmacokinetics and pharmacodynamics of the drug within different diseased patient populations. It is possible that these differences may be linked to the ability of CETP to transfer these compounds from one lipoprotein to another. This review examines the current understanding of the relationship between CETP activity and the lipoprotein distribution of a number of compounds (e.g. amphotericin B and cyclosporine A). It further suggests that additional research will expand our understanding of the role of CETP to explain other functions in lipophilic drug distribution and metabolism.  N. Ref:: 45

 

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[23]

TÍTULO / TITLE:  - Advances in transplantation tolerance.

REVISTA / JOURNAL:  - Lancet 2001 Jun 16;357(9272):1959-63.

AUTORES / AUTHORS:  - Yu X; Carpenter P; Anasetti C

INSTITUCIÓN / INSTITUTION:  - Human Immunogenetics Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

RESUMEN / SUMMARY:  - Immunosuppressive drugs developed in the past two decades have improved the short-term survival of organ allografts, but tolerance has not been achieved and almost all transplant recipients continue to require drugs throughout life. Graft rejection arises from the cognate interaction of T cells with antigen-presenting cells, the recognition of alloantigen through the T-cell receptor, and the delivery of accessory stimulation signals. Once activated by the specific antigen, replicating T cells die if they are re-exposed to the same antigen. Since depletion of antigen-activated T cells is one critical mechanism of transplantation tolerance, drugs such as ciclosporin that interfere with activation-induced T-cell death could inhibit tolerance, whereas drugs such as mycophenolate mofetil, that induce the death of activated T cells, could facilitate tolerance. Other tolerance mechanisms depend on inactivation rather than elimination of allograft reactive T cells. When antigen recognition occurs without costimulation through the CD28 and CD154 accessory receptors, or in absence of cell division, T cells become unresponsive. Thus, inhibitors of CD28 and CD154, and inhibition of T-cell division by rapamycin promotes transplantation tolerance.  N. Ref:: 54

 

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[24]

TÍTULO / TITLE:  - Clinical development of mammalian target of rapamycin inhibitors.

REVISTA / JOURNAL:  - Hematol Oncol Clin North Am 2002 Oct;16(5):1101-14.

AUTORES / AUTHORS:  - Dancey JE

INSTITUCIÓN / INSTITUTION:  - Cancer Treatment Evaluation Program, Division of Cancer Treatment and Diagnosis, Investigational Drug Branch/CTEP/DCTD/NCI, 6130 Executive Boulevard, EPN 7131, Rockville, MD 20854, USA. danceyj@ctep.nci.nih.gov

RESUMEN / SUMMARY:  - Rapamycin and CCI-779 have significant in vitro and in vivo anti-proliferative activity against a broad range of human tumor cell lines, justifying the clinical evaluation of this class of agent in cancer patients. Preliminary results from phase I studies of CCI-779 suggest that the agent is well tolerated and has anti-tumor activity. The challenge to investigators is to efficiently determine what role this class of agent will play in the treatment of cancer patients.  N. Ref:: 69

 

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[25]

TÍTULO / TITLE:  - Effects of immunosuppressive drugs on dendritic cells and tolerance induction.

REVISTA / JOURNAL:  - Transplantation 2003 May 15;75(9 Suppl):37S-42S.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000067950.90241.1D

AUTORES / AUTHORS:  - Lagaraine C; Lebranchu Y

INSTITUCIÓN / INSTITUTION:  - EA 3249, Cellules hematopoietiques, hemostase et greffe, Laboratoire d’immunologie, Faculte de medecine, Tours, France.

RESUMEN / SUMMARY:  - Dendritic cells, the most effective antigen-presenting cells for priming naive T cells and initiating immune responses, are also able to induce tolerance. This balance between immunity and tolerance depends on the functional stage of dendritic cells (DC). Activation of naive T cells by immature DC can induce tolerance. It is therefore of interest to summarize the effects of immunosuppressive agents on DC maturation and functions. In contrast to glucocorticosteroids, mycophenolate mofetil, and vitamin D(3) analogs, calcineurin inhibitors do not seem to inhibit DC maturation in in vitro culture systems. However, these molecules all appear to interfere with DC functions.  N. Ref:: 44

 

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[26]

TÍTULO / TITLE:  - Donor-specific tolerance in fully major histocompatibility major histocompatibility complex-mismatched limb allograft transplants under an anti-alphabeta T-cell receptor monoclonal antibody and cyclosporine A protocol.

REVISTA / JOURNAL:  - Transplantation 2003 Dec 27;76(12):1662-8.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000105343.49626.6F

AUTORES / AUTHORS:  - Siemionow MZ; Izycki DM; Zielinski M

INSTITUCIÓN / INSTITUTION:  - Department of Plastic Surgery, Cleveland Clinic Foundation, A60, 9500 Euclid Avenue, Cleveland, OH 44195, USA. siemiom@ccf.org

RESUMEN / SUMMARY:  - BACKGROUND: Recent studies have demonstrated that treatment with alphabeta-T-cell receptor (TCR) monoclonal antibody and cyclosporine A (CsA) can extend survival in composite tissue allografts (CTA). The purpose of this study was to induce tolerance in fully major histocompatibility complex (MHC)-mismatched rat limb allografts under 7 days of a combined alphabeta-TCR-CsA protocol. METHODS: The authors performed 30 hind-limb allotransplantations across the MHC barrier between Brown Norway donors (BN; RT1n) and Lewis recipients (LEW; RT1l). Isograft and allograft controls received no treatment. The experimental groups received monotherapy of alphabeta-TCR and CsA or a combination of alphabeta-TCR and CsA for 7 days only. Donor-specific tolerance and immunocompetence were determined by standard skin grafting in vivo and mixed lymphocyte reaction (MLR) in vitro. The efficacy of immunosuppressive therapy and the level of donor-specific chimerism were determined by flow cytometry. RESULTS: Long-term survival (>350 days) was achieved in allograft recipients (n=6) under the 7-day protocol of combined alphabeta-TCR-CsA. Donor-specific tolerance and immunocompetence of long-term chimeras were confirmed by acceptance of skin grafts from the donors and rejection of the third-party alloantigens (AxC Irish). At day 120, MLR demonstrated unresponsiveness to the host and donor antigens but strong reactivity against third-party alloantigens. Flow cytometry confirmed the high efficacy of immunosuppressive treatment and the development of donor-specific chimerism (7.6% of CD4+-RT1n+ cells, 1.3% of CD8+-RT1n+ cells, and 16.5% of CD45RA+-RT1n+ cells) in the periphery of tolerated recipients. CONCLUSIONS: Combined therapy of alphabeta-TCR-CsA for 7 days resulted in tolerance induction in fully MHC-mismatched rat hind-limb allografts. Tolerance was directly associated with stable, donor-specific chimerism.

 

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[27]

TÍTULO / TITLE:  - Dimerizer-regulated gene expression.

REVISTA / JOURNAL:  - Curr Opin Biotechnol 2002 Oct;13(5):459-67.

AUTORES / AUTHORS:  - Pollock R; Clackson T

INSTITUCIÓN / INSTITUTION:  - ARIAD Gene Therapeutics, 26 Landsdowne Street, Cambridge, MA 02139, USA. roy.pollock@ariad.com

RESUMEN / SUMMARY:  - Control of gene expression using small molecules is a powerful research tool and has clinical utility in the context of regulated gene therapy. Use of chemical inducers of dimerization, or dimerizers, for this purpose has several advantages, including tight regulation, modularity to facilitate iterative improvements, and assembly from human proteins to minimize immune responses in clinical applications. Recent developments include the use of the rapamycin-based dimerizer system to regulate the expression of endogenous genes, the generation of new chemical dimerizers based on FK506, dexamethasone and methotrexate, and progress towards the clinical use of adeno-associated virus and adenovirus vectors regulated by rapamycin analogs.  N. Ref:: 40

 

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[28]

TÍTULO / TITLE:  - Posttransplantation diabetes: a systematic review of the literature.

REVISTA / JOURNAL:  - Diabetes Care. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://care.diabetesjournals.org/ 

      ●● Cita: Diabetes Care: <> 2002 Mar;25(3):583-92.

AUTORES / AUTHORS:  - Montori VM; Basu A; Erwin PJ; Velosa JA; Gabriel SE; Kudva YC

INSTITUCIÓN / INSTITUTION:  - Division of Endocrinology, Diabetes, Metabolism, Nutrition, and Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.

RESUMEN / SUMMARY:  - OBJECTIVES: To systematically review the incidence of posttransplantation diabetes (PTD), risk factors for its development, prognostic implications, and optimal management. RESEARCH DESIGN AND METHODS: We searched databases (MEDLINE, EMBASE, the Cochrane Library, and others) from inception to September 2000, reviewed bibliographies in reports retrieved, contacted transplantation experts, and reviewed specialty journals. Two reviewers independently determined report inclusion (original studies, in all languages, of PTD in adults with no history of diabetes before transplantation), assessed study methods, and extracted data using a standardized form. Meta-regression was used to explain between-study differences in incidence. RESULTS: Nineteen studies with 3,611 patients were included. The 12-month cumulative incidence of PTD is lower (<10% in most studies) than it was 3 decades ago. The type of immunosuppression explained 74% of the variability in incidence (P = 0.0004). Risk factors were patient age, nonwhite ethnicity, glucocorticoid treatment for rejection, and immunosuppression with high-dose cyclosporine and tacrolimus. PTD was associated with decreased graft and patient survival in earlier studies; later studies showed improved outcomes. Randomized trials of treatment regimens have not been conducted. CONCLUSIONS: Physicians should consider modification of immunosuppressive regimens to decrease the risk of PTD in high-risk transplant recipients. Randomized trials are needed to evaluate the use of oral glucose-lowering agents in transplant recipients, paying particular attention to interactions with immunosuppressive drugs.  N. Ref:: 79

 

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[29]

TÍTULO / TITLE:  - Calcineurin inhibition and cardiac hypertrophy: a matter of balance.

REVISTA / JOURNAL:  - Proc Natl Acad Sci U S A. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.pnas.org/ 

      ●● Cita: Proc Natl Acad Sci USA (PNAS): <> 2001 Mar 13;98(6):2947-9.

      ●● Enlace al texto completo (gratuito o de pago) 1073/pnas.051033698

AUTORES / AUTHORS:  - Leinwand LA

INSTITUCIÓN / INSTITUTION:  - Department of Molecular, Cellular, and Developmental Biology, Porter Addition, Room A3B40, University of Colorado, Boulder, CO 80309-0347, USA. leinwand@stripe.colorado.edu  N. Ref:: 18

 

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[30]

TÍTULO / TITLE:  - The fission yeast TOR proteins and the rapamycin response: an unexpected tale.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2004;279:85-95.

AUTORES / AUTHORS:  - Weisman R

INSTITUCIÓN / INSTITUTION:  - Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel-Aviv University, 69978 Tel-Aviv, Israel. ronitt@post.tau.ac.il

RESUMEN / SUMMARY:  - The TOR proteins are known as key regulators of cell growth in response to nutritional and mitogenic signals and as targets for the immunosuppressive and anti-cancerous drug rapamycin. The fission yeast Schizosaccharomyces pombe has two TOR homologues, tor1+ and tor2+. Despite their structural similarity, these genes have distinct functions: tor1+ is required under starvation, extreme temperatures, and osmotic or oxidative stress conditions, whereas tor2+ is required under normal growth conditions. Surprisingly, rapamycin does not seem to inhibit the S. pombe TOR-related functions. Rapamycin specifically inhibits sexual development in S. pombe, and this seems to stem from direct inhibition of the S. pombe FKBP12 homologue. Why S. pombe cells are resistant to rapamycin during the growth phase is as yet unclear and awaits further analysis of the TOR-dependent signaling pathways.  N. Ref:: 27

 

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[31]

TÍTULO / TITLE:  - Renal function as a predictor of long-term graft survival in renal transplant patients.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2003 May;18 Suppl 1:i3-6.

AUTORES / AUTHORS:  - First MR

INSTITUCIÓN / INSTITUTION:  - Research and Development, Fujisawa Healthcare, Inc., Deerfield, IL 60015, USA. roy_first@fujisawa.com

RESUMEN / SUMMARY:  - Acute rejection is a major risk factor for kidney graft failure. However, as acute rejection has been progressively reduced by recent immunosuppressive regimens, other risk factors are becoming increasingly important. Evidence is accumulating that early renal function predicts long-term outcome. A recent registry survey of more than 100 000 kidney transplants found that 6- and 12-month serum creatinine levels, as well as the change between 6 and 12 months, are strongly associated with long-term graft survival. A survey of paediatric renal transplant recipients showed that poor creatinine clearance (<50 ml/min) as early as 30 days post-transplant predicted an annual rate of graft loss of 13% compared with <3% in patients with 30-day clearance >50 ml/min. This association between early renal function and long-term outcome was confirmed in multicentre studies. Renal transplant recipients (n=572) with 6-month serum creatinine levels >1.5 mg/dl suffered 3-year graft loss of 19.3% compared with only 8.5% in patients with levels <1.6 mg/dl (P<0.001). Significantly fewer patients receiving tacrolimus had 12-month serum creatinine levels >1.5 mg/dl compared with cyclosporin (42 versus 54%, P<0.05). Interestingly, a single-centre study (n=436) found that while glomerular filtration rate (GFR) at 6 months post-transplant had remained stable over the last decade, the rate of loss of renal function had decreased. A lower rate of GFR loss was associated with absence of rejection, use of mycophenolate mofetil rather than azathioprine and use of tacrolimus rather than cyclosporin (P<0.01). In conclusion, early measures of renal function allow identification of those patients at highest risk of graft failure and provide an invaluable tool for improving outcomes by tailored immunosuppression. The choice of such immunosuppression should be guided not only by its ability to prevent rejection, but also by its impact on renal function.  N. Ref:: 11

 

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[32]

TÍTULO / TITLE:  - Mycophenolate mofetil for the prevention and treatment of graft-versus-host disease following stem cell transplantation: preliminary findings.

REVISTA / JOURNAL:  - Bone Marrow Transplant 2001 Jun;27(12):1255-62.

AUTORES / AUTHORS:  - Vogelsang GB; Arai S

INSTITUCIÓN / INSTITUTION:  - Department of Oncology, Johns Hopkins Oncology Center, Baltimore, MD 21287-8943, USA.

RESUMEN / SUMMARY:  - The therapeutic benefits of allogeneic stem cell transplantation in patients with hematologic disorders are limited by the significant morbidity and mortality of graft-versus-host disease (GVHD). Current agents for the prevention and treatment of GVHD have limited efficacy and often result in toxic side-effects. Mycophenolate mofetil (MMF) is a new immunosuppressant with a selective mechanism of action. When employed following solid organ transplantation, MMF reduces the incidence and severity of acute rejection episodes. By selectively targeting activated lymphocytes, the active metabolite of MMF, mycophenolic acid (MPA), appears to augment the actions of standard immunosuppressant agents without adding overlapping toxicities. Studies of combination regimens that include MMF report that this agent permits a dose reduction of cyclosporine, tacrolimus, or corticosteroid, without increasing the incidence of acute rejection in solid organ transplants. Reports on the efficacy of MMF following stem cell transplantation in animal studies were mixed. However, the use of a non-myeloablative conditioning regimen with a post-graft immunosuppressive regimen of MMF and cyclosporine was able to sustain stable mixed chimeras in 60% to 80% of dogs who received hematopoietic grafts from DLA-identical littermates. MMF has demonstrated activity in preliminary clinical trials for GVHD prophylaxis, and treatment of acute or chronic GVHD. Larger clinical trials are warranted to determine the optimum dose and route of MMF administration for GVHD, as well as the comparative safety and efficacy of MMF-containing regimens.  N. Ref:: 36

 

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[33]

TÍTULO / TITLE:  - Composite tissue allotransplantation in chimeric hosts part II. A clinically relevant protocol to induce tolerance in a rat model.

REVISTA / JOURNAL:  - Transplantation 2003 Dec 15;76(11):1548-55.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000085288.12571.65

AUTORES / AUTHORS:  - Prabhune KA; Gorantla VS; Perez-Abadia G; Francois CG; Vossen M; Laurentin-Perez LA; Breidenbach WC; Wang GG; Anderson GL; Pidwell DJ; Barker JH; Maldonado C

INSTITUCIÓN / INSTITUTION:  - Division of Plastic and Reconstructive Surgery, University of Louisville, Louisville, Kentucky, USA.

RESUMEN / SUMMARY:  - BACKGROUND: We and others have shown that mixed allogeneic chimerism induces donor-specific tolerance to composite tissue allografts across major histocompatibility complex barriers without the need for immunosuppression. However, a delay period between bone marrow transplantation and limb allotransplantation is required, making such protocols impractical for clinical application. This study eliminates this delay period in a rat hind limb allotransplantation model by performing mixed allogeneic chimerism induction and transplantation “simultaneously.” METHODS: Group 1 included controls in which naive Wistar Furth (WF) hosts received ACI hind limbs. Group 2 included (ACI-->WF) chimeras that received limbs from third-party donors (Fisher), and group 3 included chimeras that received irradiated (1,050 cGy) ACI limbs. In group 4, WF hosts conditioned with 950 cGy received irradiated (1,050 cGy) ACI limbs followed by infusion of 100 x 10(6) ACI T-cell-depleted bone marrow cells and immunotherapy (tacrolimus and mycophenolate mofetil) for 28 days. Group 5 animals received the same treatment as group 4 animals without immunotherapy. RESULTS: The rats in groups 1 and 2 rejected their limbs within 10 days. Only one rat in group 4 survived to the end of the study. Groups 3 and 5 demonstrated long-term limb survival without rejection or graft-versus-host disease. High levels of donor chimerism (>80%) were achieved and maintained throughout the study. Mixed lymphocyte reaction assays in both groups revealed donor-specific hyporesponsiveness with vigorous third-party reactivity. CONCLUSIONS: This study demonstrated that infusion of donor bone marrow cells into conditioned hosts immediately after limb transplantation results in stable mixed chimerism, robust tolerance, and reliable limb allograft survival.

 

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[34]

TÍTULO / TITLE:  - Tacrolimus ointment for the treatment of atopic dermatitis: clinical and pharmacologic effects.

REVISTA / JOURNAL:  - Allergy Asthma Proc 2002 May-Jun;23(3):191-7.

AUTORES / AUTHORS:  - Rico MJ; Lawrence I

INSTITUCIÓN / INSTITUTION:  - Fujisawa Healthcare, Inc, 3 Pookway North Deerfeild, IL 60022, USA.

RESUMEN / SUMMARY:  - The topical immunomodulator tacrolimus ointment has been shown to be safe and effective in the treatment of atopic dermatitis in clinical trials involving over 16,000 patients. Clinical trial results focusing on tacrolimus’ safety and efficacy are summarized. Minimal systemic absorption results from topical application in patients with atopic dermatitis. Although the exact mechanism of action of tacrolimus ointment in atopic dermatitis is unknown, tacrolimus is known to inhibit up-regulation of cytokine production following T cell activation and to decrease Fc epsilon RI expression on dendritic antigen-presenting cells in skin. Additional mechanisms of action of tacrolimus relevant in the pathogenesis of inflammatory skin disorders are discussed.  N. Ref:: 27

 

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[35]

TÍTULO / TITLE:  - mTOR as a positive regulator of tumor cell responses to hypoxia.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2004;279:299-319.

AUTORES / AUTHORS:  - Abraham RT

INSTITUCIÓN / INSTITUTION:  - Program in Signal Transduction Research, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA. abraham@burnham.org

RESUMEN / SUMMARY:  - Rapamycin is a clinically approved immunosuppressive agent that has recently shown promising antitumor activities in human patients. In contrast to many conventional chemotherapeutic agents, rapamycin displays a remarkably high level of selectivity for certain types of tumors. The pharmacological activities of rapamycin are attributable to the functional inhibition of a single target protein, termed the mammalian target of rapamycin (mTOR). Because mTOR is widely expressed in both normal and transformed cells, variations in mTOR expression levels are likely not a primary determinant of tumor sensitivity to rapamycin. However, recent studies highlighted an intriguing link between cancer cell sensitivity to rapamycin and deregulated signaling through the phosphoinositide (PI) 3-kinase pathway. These findings have prompted a search for cancer-related responses that are jointly regulated by the PI 3-kinase signaling cascade and mTOR. The oxygen-regulated transcription factor, hypoxia-induced factor (HIF)-1, has emerged as a candidate target for both of these two highly interactive signaling proteins. Here we review evidence that mTOR functions as a positive regulator of HIF-1-dependent responses to hypoxic stress in human cancer cells.  N. Ref:: 71

 

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[36]

TÍTULO / TITLE:  - TGF-beta expression in protocol transplant liver biopsies: a comparative study between cyclosporine-A (CyA) and tacrolimus (FK 506) immunosuppression.

REVISTA / JOURNAL:  - Transplant Proc 2001 Feb-Mar;33(1-2):1378-80.

AUTORES / AUTHORS:  - Mohamed MA; Burt AD; Robertson H; Kirby JA; Talbot D

INSTITUCIÓN / INSTITUTION:  - Transplant Immunobiology Group, Department of Surgery, University of Newcastle, NE2 4HH, Newcastle Upon Tyne, UK.

 

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[37]

TÍTULO / TITLE:  - Review article: does the use of immunosuppressive therapy in inflammatory bowel disease increase the risk of developing lymphoma?

REVISTA / JOURNAL:  - Aliment Pharmacol Ther 2001 Dec;15(12):1843-9.

AUTORES / AUTHORS:  - Bebb JR; Logan RP

INSTITUCIÓN / INSTITUTION:  - Division of Gastroenterology, University Hospital, Nottingham, UK.

RESUMEN / SUMMARY:  - Recent case reports have raised concerns regarding the risks of non-Hodgkin’s lymphoma in patients with inflammatory bowel disease treated with immunosuppressive agents. This evidence-based review examines this issue from data derived from the use of immunosuppression in other conditions (and inflammatory bowel disease). We conclude that, in transplant (cardiac and renal) recipients, immunosuppression increases the risk of non-Hodgkin’s lymphoma. For non-transplant patients (with psoriasis and rheumatoid arthritis), debate remains as to whether the observed increase in the incidence of non-Hodgkin’s lymphoma is due to drug or disease. For inflammatory bowel disease per se, population studies show no significant increase in the risk of non-Hodgkin’s lymphoma, with a relative risk of 1.3 (95% confidence interval, 0.9-1.7) compared to expected rates, and several studies of immuno- suppression in inflammatory bowel disease do not appear to confirm a significant rate of lymphoma incidence. Reported cases of lymphoma from single centres should be viewed with caution as evidence of increased risk. If any association exists, it is likely to be of minimal clinical significance compared to the established and more frequent risks of myelosuppression and infection, and is unlikely to outweigh the benefit of immunosuppression in inflammatory bowel disease.  N. Ref:: 43

 

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[38]

TÍTULO / TITLE:  - CD30+ T-cell lymphoma in a patient with psoriasis treated with ciclosporin and infliximab.

REVISTA / JOURNAL:  - Br J Dermatol 2003 Jul;149(1):170-3.

AUTORES / AUTHORS:  - Mahe E; Descamps V; Grossin M; Fraitag S; Crickx B

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Bichat-Claude Bernard Hospital, 46 Rue Henri-Huchard, Paris Cedex 18, France. emmanuel.mahe@bch.ap-hop-paris.fr

RESUMEN / SUMMARY:  - There is a known relationship between the use of immunosuppressive therapies and the development of lymphoproliferative malignancies. These lymphomas are mainly B-cell nonHodgkin’s lymphomas associated with Epstein-Barr virus. Most cases concern classical immunosuppressive treatments including ciclosporin and methotrexate. A relationship between the new antitumour necrosis factor (TNF)-alpha agents and lymphoproliferative malignancies is debated. Patients with psoriasis on immunosuppressive therapies, mainly ciclosporin, are considered to have a low risk of developing lymphoid proliferation. We report a patient with erythrodermic psoriasis treated with ciclosporin and infliximab who developed a CD30+ T-cell lymphoma. This lymphoma regressed after stopping these treatments. In this case, the anti-TNF-alpha agent may have played a role in association with ciclosporin in the development of the lymphoproliferative disorder. Whereas the combination of anti-TNF-alpha therapies with methotrexate has been well studied, their combination with ciclosporin has been evaluated only in a few patients. Psoriatic patients who may require anti-TNF-alpha treatment have often been or will be treated with ciclosporin. The combination of ciclosporin and anti-TNF-alpha warrants further investigation.  N. Ref:: 17

 

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[39]

TÍTULO / TITLE:  - European best practice guidelines for renal transplantation. Section IV: Long-term management of the transplant recipient. IV.5.8. Cardiovascular risks. Immunosuppressive therapy.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2002;17 Suppl 4:30-1.

RESUMEN / SUMMARY:  - GUIDELINE: Immunosuppressive therapies, especially corticosteroids and anticalcineurin inhibitors; contribute to the prevalence of cardiovascular risk factors, such as arterial hypertension, hyperlipidaemia and hyperglycaemia, and this effect is dose dependent. Reduction of the dose, withdrawal and/or switching to another drug could be useful to control these risk factors.

 

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[40]

TÍTULO / TITLE:  - Cyclosporin trough levels: is monitoring necessary during short-term treatment in psoriasis? A systematic review and clinical data on trough levels.

REVISTA / JOURNAL:  - Br J Dermatol 2002 Jul;147(1):122-9.

AUTORES / AUTHORS:  - Heydendael VM; Spuls PI; Ten Berge IJ; Opmeer BC; Bos JD; de Rie MA

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Academic Medical Center, University of Amsterdam, PO Box 22660, the Netherlands.

RESUMEN / SUMMARY:  - BACKGROUND: Cyclosporin is an effective treatment for severe plaque psoriasis. Unfortunately, its use may be limited by time- and dose-related nephrotoxicity. Serum trough levels may be useful for monitoring the risk of nephrotoxicity. OBJECTIVES: To determine whether monitoring of trough levels is necessary in psoriasis patients undergoing short-term treatment with cyclosporin. METHODS: A computerized and manual literature search identified studies on adults with plaque-type psoriasis treated with cyclosporin < or = 5 mg kg-1 daily, in which trough levels were measured in whole blood. Number of patients, treatment duration, formulation and dosage, renal function tests and trough levels were extracted. The association between renal function and trough levels was investigated. Additionally, in a randomized controlled trial on cyclosporin vs. methotrexate in moderate to severe psoriasis, cyclosporin trough levels were measured frequently in 20 patients during 12 weeks of treatment. The Pearson correlation coefficient between serum creatinine and cyclosporin trough levels was calculated. RESULTS: Fifty-six articles were found concerning cyclosporin trough level measurements in psoriasis patients, of which eight were analysed. Many studies were excluded due to inappropriate cyclosporin dosages used. As data were heterogeneous and lacked various key parameters, a correlation study and a meta-analysis could not be performed. Instead, a quantitative description of the literature was given. No high mean trough levels or elevations of serum creatinine were described. In our clinical study, all the mean trough levels in 17 patients treated with cyclosporin 3 mg kg-1 daily were within the therapeutic range (< 200 ng mL-1). Elevated trough levels were found in two of three patients treated with cyclosporin 3-5 mg kg-1 daily. No signs of renal dysfunction were seen. CONCLUSIONS: The literature does not provide a definitive answer on whether monitoring cyclosporin trough levels in patients with psoriasis should be standard practice. Our own data show no need for cyclosporin trough level monitoring during short-term treatment with cyclosporin 3 mg kg-1 daily. However, when cyclosporin doses are > 3 mg kg-1 daily, monitoring may be indicated.  N. Ref:: 32

 

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[41]

TÍTULO / TITLE:  - Elucidating TOR signaling and rapamycin action: lessons from Saccharomyces cerevisiae.

REVISTA / JOURNAL:  - Microbiol Mol Biol Rev. - Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://mmbr.asm.org/ 

      ●● Cita: Microbiology & Molecular Biology Reviews: <> 2002 Dec;66(4):579-91, table of contents.

AUTORES / AUTHORS:  - Crespo JL; Hall MN

INSTITUCIÓN / INSTITUTION:  - Division of Biochemistry, Biozentrum, University of Basel, CH-4056 Basel, Switzerland.

RESUMEN / SUMMARY:  - TOR (target of rapamycin) is a phosphatidylinositol kinase-related protein kinase that controls cell growth in response to nutrients. Rapamycin is an immunosuppressive and anticancer drug that acts by inhibiting TOR. The modes of action of TOR and rapamycin are remarkably conserved from S. cerevisiae to humans. The current understanding of TOR and rapamycin is derived largely from studies with S. cerevisiae. In this review, we discuss the contributions made by S. cerevisiae to understanding rapamycin action and TOR function.  N. Ref:: 171

 

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[42]

TÍTULO / TITLE:  - Structures of calcineurin and its complexes with immunophilins-immunosuppressants.

REVISTA / JOURNAL:  - Biochem Biophys Res Commun 2003 Nov 28;311(4):1095-102.

AUTORES / AUTHORS:  - Ke H; Huai Q

INSTITUCIÓN / INSTITUTION:  - Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, The University of North Carolina, Chapel Hill, NC 27599-7260, USA. hke@med.unc.edu

RESUMEN / SUMMARY:  - Calcineurin (CN) is a Ca(2+)/calmodulin-dependent serine/threonine protein phosphatase and is involved in many physiological processes such as T-cell activation and cardiac hypertrophy. The crystal structures of CN and its complexes with FKBP12-FK506 and cyclophilin-cyclosporin showed that the two structurally unrelated immunophilins-immunosuppressants bind to a common composite surface made up of the residues from both catalytic subunit and regulatory subunit of CN. The recognition of the immunophilins and immunosuppressive drugs is achieved by common but few distinct CN residues. However, the binding pattern of FKBP12-FK506 such as hydrogen bonding is significantly different from that of CyPA-CsA. This common but distinct recognition may indicate capacity of the composition surface for binding of other inhibitory proteins. The recognition site and the active site are adjacent and form an “L” shaped cleft. This implies that the immunophilin recognition site may also serve as a recognition site to define the narrow substrate specificity of calcineurin.  N. Ref:: 61

 

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[43]

TÍTULO / TITLE:  - European best practice guidelines for renal transplantation. Section IV: Long-term management of the transplant recipient. IV.3.2. Long-term immunosuppression. Therapy conversion.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2002;17 Suppl 4:20-1.

RESUMEN / SUMMARY:  - GUIDELINE: Conversion of immunosuppressive drug therapy is recommended to avoid or reduce drug-specific adverse effects, and is generally safe for long-term graft outcome.

 

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[44]

TÍTULO / TITLE:  - Multidrug resistance reversal agents.

REVISTA / JOURNAL:  - J Med Chem 2003 Nov 6;46(23):4805-17.

      ●● Enlace al texto completo (gratuito o de pago) 1021/jm030183a

AUTORES / AUTHORS:  - Robert J; Jarry C

INSTITUCIÓN / INSTITUTION:  - Institut Bergonie, 229, Cours de l’Argonne, 33076 Bordeaux Cedex, France. robert@bergonie.org  N. Ref:: 151

 

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[45]

TÍTULO / TITLE:  - Prospects for treatment of paraquat-induced lung fibrosis with immunosuppressive drugs and the need for better prediction of outcome: a systematic review.

REVISTA / JOURNAL:  - Qjm. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://qjmed.oupjournals.org/ 

      ●● Cita: QJM: <> 2003 Nov;96(11):809-24.

AUTORES / AUTHORS:  - Eddleston M; Wilks MF; Buckley NA

INSTITUCIÓN / INSTITUTION:  - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, UK. eddlestonm@eureka.lk

RESUMEN / SUMMARY:  - BACKGROUND: Acute paraquat self-poisoning is a significant problem in parts of Asia, the Pacific and the Caribbean. Ingestion of large amounts of paraquat results in rapid death, but smaller doses often cause a delayed lung fibrosis that is usually fatal. Anti-neutrophil (‘immunosuppressive’) treatment has been recommended to prevent lung fibrosis, but there is no consensus on efficacy. Aim: To review the evidence for the use of immunosuppression in paraquat poisoning, and to identify validated prognostic systems that would allow the use of data from historical control studies and the future identification of patients who might benefit from immunosuppression. DESIGN:Systematic review. METHODS: We searched PubMed, Embase and Cochrane databases for ‘paraquat’ together with ‘poisoning’ or ‘overdose’. We cross-checked references and contacted experts, and searched on [www.google.com] and [www.yahoo.com] using ‘paraquat’, ‘cyclophosphamide’, ‘methylprednisolone’ and ‘prognosis’. RESULTS: We found ten clinical studies of immunosuppression in paraquat poisoning. One was a randomized controlled trial (RCT). Seven used historical controls only; the other two were small (n = 1 and n = 4). Mortality in controls and patients varied markedly between studies. Three of the seven non-RCT controlled studies measured plasma paraquat; analysis using Proudfoot’s or Hart’s nomograms did not suggest that immunosuppression increased survival in these studies. Of 16 prognostic systems for paraquat poisoning, none has been independently validated in a large cohort. DISCUSSION: The authors of the RCT have performed valuable and difficult research, but their results are hypothesis-forming rather than conclusive; elsewhere, the use of historical controls is problematic. In the absence of a validated prognostic marker, a large RCT of immunosuppression using death as the primary outcome is required. This RCT should also prospectively test and validate the available prognostic methods, so that future patients can be selected for this and other therapies on admission.  N. Ref:: 57

 

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[46]

TÍTULO / TITLE:  - Prevention by dietary (n-6) polyunsaturated phosphatidylcholines of intrahepatic cholestasis induced by cyclosporine A in animals.

REVISTA / JOURNAL:  - Life Sci 2003 Jun 13;73(4):381-92.

AUTORES / AUTHORS:  - Chanussot F; Benkoel L

INSTITUCIÓN / INSTITUTION:  - INSERM U. 476, Faculte de Medecine, 27 bd Jean Moulin, 13385 Marseille cedex 05, France. Francoise.Chanussot@medecine.univ-mrs.fr

RESUMEN / SUMMARY:  - Previous findings showed that dietary (n-6) polyunsaturated phosphatidylcholines (vegetable lecithin) could efficiently prevent intrahepatic cholestasis induced by cyclosporine A in rats. Mechanistic studies showed that expressions in rat liver of Na(+), K(+)-ATPase, Ca(2+), Mg(2+)-ATPase and F-actin were both decreased by drug administration and both enhanced by (n-6) lecithin enriched diet. There is a possible direct effect of phosphatidylcholines, vectors of polyunsaturated fatty acids provided by the metabolism of the dietary lecithin, on the aforesaid hepatic parameters. Such modulations by drug and diet result in reversed modifications of membrane composition and fluidity. Final outcome is decreased and enhanced bile lipid secretion by cyclosporine and vegetable lecithin enriched diet respectively. Moreover, we advance the hypothesis of a bypass process including a separate and functional actin-independent way for the non micellar and phospholipid-dependent secretion of bile lipids. The relationships between the ATPases, the microfilament components such as F-actin and the different transporters still remain to be clarified. Furthermore, one can speculate on beneficial effects in humans of diets enriched in vegetable lecithins that might prevent cholestasis induced by cyclosporine A.  N. Ref:: 75

 

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[47]

TÍTULO / TITLE:  - Review article: the risk of lymphoma associated with inflammatory bowel disease and immunosuppressive treatment.

REVISTA / JOURNAL:  - Aliment Pharmacol Ther 2001 Aug;15(8):1101-8.

AUTORES / AUTHORS:  - Aithal GP; Mansfield JC

INSTITUCIÓN / INSTITUTION:  - Department of Gastroenterology, University of Newcastle, Newcastle upon Tyne, UK.

RESUMEN / SUMMARY:  - Lymphoma complicating inflammatory bowel disease is well described. Whether the risk of lymphoma is increased by immunosuppressive treatment with azathioprine, 6-mercaptopurine or infliximab is a common concern among patients and physicians considering using these agents. This review aims to quantify the lymphoma risk in inflammatory bowel disease and the added risk attributable to these treatments. The evidence from published cases is that lymphomas occur at sites of active inflammatory bowel disease more often than expected for this to be a chance association. Studies on inflammatory bowel disease populations are conflicting, with some follow-up studies from large inflammatory bowel disease clinics showing an increase in lymphoma incidence, while other population-based studies show little or no increase in risk of lymphoma. A small increase in lymphoma risk in inflammatory bowel disease, perhaps 2-3-fold, may be compatible with both sets of data. Studies of the risks associated with immuno- suppression are less satisfactory, with smaller numbers of patients and relatively short follow-up. The available evidence would support a further increase in lymphoma risk associated with immunosuppressive treatment in inflammatory bowel disease of around fivefold compared to no immunosuppressive use, and tenfold compared to the general population. The risks appear to be less than that associated with renal and hepatic transplant-related immunosuppression. Infliximab treatment is still too new to make a full assessment of its long-term safety, but post-marketing surveillance currently suggests that lymphoma risk may not be any greater than that associated with azathioprine and 6-mercaptopurine. Population-wide surveillance for lymphoma in inflammatory bowel disease would be required to narrow the confidence intervals on these estimates of lymphoma risk in inflammatory bowel disease and immunosuppressive treatment.  N. Ref:: 54

 

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[48]

TÍTULO / TITLE:  - Patient management by Neoral C(2) monitoring: an international consensus statement.

REVISTA / JOURNAL:  - Transplantation 2002 May 15;73(9 Suppl):S12-8.

AUTORES / AUTHORS:  - Levy G; Thervet E; Lake J; Uchida K

INSTITUCIÓN / INSTITUTION:  - Multiorgan Transplant Program, Toronto General Hospital, 621 University Avenue, 10NU-116, Toronto, Ontario M5G 2C4, Canada.  N. Ref:: 36

 

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[49]

TÍTULO / TITLE:  - Calcineurin inhibitor-free CD28 blockade-based protocol protects allogeneic islets in nonhuman primates.

REVISTA / JOURNAL:  - Diabetes. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://diabetes.diabetesjournals.org/ 

      ●● Cita: Diabetes: <> 2002 Feb;51(2):265-70.

AUTORES / AUTHORS:  - Adams AB; Shirasugi N; Durham MM; Strobert E; Anderson D; Rees P; Cowan S; Xu H; Blinder Y; Cheung M; Hollenbaugh D; Kenyon NS; Pearson TC; Larsen CP

INSTITUCIÓN / INSTITUTION:  - Emory Transplant Center, Department of Surgery, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

RESUMEN / SUMMARY:  - Recent success using a steroid-free immunosuppressive regimen has renewed enthusiasm for the use of islet transplantation to treat diabetes. Toxicities associated with the continued use of a calcineurin inhibitor may limit the wide-spread application of this therapy. Biological agents that block key T-cell costimulatory signals, in particular the CD28 pathway, have demonstrated extraordinary promise in animal models. LEA29Y (BMS-224818), a mutant CTLA4-Ig molecule with increased binding activity, was evaluated for its potential to replace tacrolimus and protect allogeneic islets in a preclinical primate model. Animals received either the base immunosuppression regimen (rapamycin and anti-IL-2R monoclonal antibody [mAb]) or the base immunosuppression and LEA29Y. Animals receiving the LEA29Y/rapamycin/anti-IL-2R regimen (n = 5) had significantly prolonged islet allograft survival (204, 190, 216, 56, and >220 days). In contrast, those animals receiving the base regimen alone (n = 2) quickly rejected the transplanted islets at 1 week (both at 7 days). The LEA29Y-based regimen prevented the priming of anti-donor T- and B-cell responses, as detected by interferon-gamma enzyme-linked immunospot and allo-antibody production, respectively. The results of this study suggest that LEA29Y is a potent immunosuppressant that can effectively prevent rejection in a steroid-free immunosuppressive protocol and produce marked prolongation of islet allograft survival in a preclinical model.

 

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[50]

TÍTULO / TITLE:  - A benefit-risk assessment of basiliximab in renal transplantation.

REVISTA / JOURNAL:  - Drug Saf. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.csmwm.org/ 

      ●● Cita: Drug Safety: <> 2004;27(2):91-106.

AUTORES / AUTHORS:  - Boggi U; Danesi R; Vistoli F; Del Chiaro M; Signori S; Marchetti P; Del Tacca M; Mosca F

INSTITUCIÓN / INSTITUTION:  - Division of General Surgery and Transplants, Department of Oncology, Transplants and Advanced Technologies in Medicine, University of Pisa, Pisa, Italy. uboggi@med.unipi.it

RESUMEN / SUMMARY:  - Interleukin-2 (IL-2) and its receptor (IL-2R) play a central role in T lymphocyte activation and immune response after transplantation. Research on the biology of IL-2R allowed the identification of key signal transduction pathways involved in the generation of proliferative and antiapoptotic signals in T cells. The alpha-chain of the IL-2R is a specific peptide against which monoclonal antibodies have been raised, with the aim of blunting the immune response by means of inhibiting proliferation and inducing apoptosis in primed lymphocytes. Indeed, basiliximab, one of such antibodies, has proved to be effective in reducing the episodes of acute rejection after kidney and pancreas transplantation. The use of basiliximab was associated with a significant reduction in the incidence of any treated rejection episodes after kidney transplantation in the two major randomised studies (placebo 52.2% vs basiliximab 34.2% at 6 months, European study; placebo 54.9% vs basiliximab 37.6% at 1 year, US trial). Basiliximab and equine antithymocyte globulin (ATG) administration resulted in a similar rate of biopsy-proven acute rejection at 6 months (19% for both) and at 12 months (19% and 20%, respectively). The use of basiliximab appears not to be associated with an increased incidence of adverse events as compared with placebo in immunosuppressive regimens, including calcineurin inhibitors, mycophenolate mofetil or azathioprine and corticosteroids, and its safety profile is superior to ATG. Moreover, a similar occurrence of infections is noted in selected studies (65.5% after basiliximab vs 65.7% of controls), including cytomegalovirus infection (17.3% vs 14.5%), and cytokine-release syndrome is not observed. Finally, economic analysis demonstrated lower costs of overall treatment in patients treated with basiliximab. Therefore, the use of basiliximab entails a very low risk, allows safe reduction of corticosteroid dosage and reduces the short- and mid-term rejection rates. However, the improvement in the long-term survival of kidney grafts in patients treated according to modern immunosuppressive protocols is still to be demonstrated. These conclusions are based on a systematic review of the scientific literature, indexed on Medline database, concerning the mechanism of action, therapeutic activity, safety and pharmacoeconomic evaluation of basiliximab in renal transplantation.  N. Ref:: 62

 

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[51]

TÍTULO / TITLE:  - New immunosuppressive agent: expectations and controversies.

REVISTA / JOURNAL:  - Transplantation 2003 Mar 27;75(6):741-2.

AUTORES / AUTHORS:  - Alsina J; Grinyo JM

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Bellvitge Hospital, Barcelona, España.  N. Ref:: 5

 

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[52]

TÍTULO / TITLE:  - Role of leucine in the regulation of mTOR by amino acids: revelations from structure-activity studies.

REVISTA / JOURNAL:  - J Nutr. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.nutrition.org/ 

      ●● Cita: Journal of Nutrition: <> 2001 Mar;131(3):861S-865S.

AUTORES / AUTHORS:  - Lynch CJ

INSTITUCIÓN / INSTITUTION:  - Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA. clynch@psu.edu

RESUMEN / SUMMARY:  - In this study an overview is presented of the mTOR signaling pathway and its regulation by amino acids, particularly L-leucine. Our laboratory is studying amino acid regulation of mTOR in adipocytes. Potential roles for mTOR in adipocytes that were previously posited include hypertrophic growth, leptin secretion, protein synthesis and adipose tissue morphogenesis. A current area of interest in the field is how amino acids regulate mTOR and which amino acids are regulatory. Revelations concerning mechanism and recognition are emerging from different laboratories that examined the structural requirements for stimulation and inhibition of the mTOR signaling pathway by leucine and amino acid analogs. In adipocytes and some other cell types, leucine appears to be the main regulatory amino acid. However, this is not uniformly the case. In those cells where mTOR is regulated by several amino acids, there is evidence that the mechanism of mTOR activation may be different from cells where mainly leucine is regulatory. Furthermore, in tissues where leucine regulates mTOR, the possible existence of different tissue-specific leucine recognition sites may be indicated.  N. Ref:: 47

 

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[53]

TÍTULO / TITLE:  - Drug-eluting stents and glycoprotein IIb/IIIa inhibitors: combination therapy for the future.

REVISTA / JOURNAL:  - Am Heart J 2003 Oct;146(4 Suppl):S13-7.

      ●● Enlace al texto completo (gratuito o de pago) 1016/j.ahj.2003.09.004

AUTORES / AUTHORS:  - Leon MB; Bakhai A

RESUMEN / SUMMARY:  - BACKGROUND: Although coronary stenting has improved the results of coronary interventions compared to coronary angioplasty alone, in-stent restenosis remains a significant limitation of this procedure. Drug-eluting stents with or without glycoprotein IIb/IIIa inhibitor therapy represent an additional advance in the evolution of this strategy. METHODS: We review the currently available trials comparing studies of non-drug-eluting and drug-eluting stents using sirolimus and paclitaxel agents and their derivatives. RESULTS: Ten studies are available that compare drug-eluting to traditional non-drug-eluting stents. A variety of antiplatelet regimes have been used. The majority of these studies are in the process of being published. No head-to-head studies comparing different drug-eluting stents are available. CONCLUSIONS: Drug-eluting stents using sirolimus and paclitaxel in combination with enhanced antiplatelet strategies represent an important advantage over non-drug-eluting stents for the reduction of in-stent restenosis. The rate at which drug-eluting stents are adapted into widespread practice depends heavily on whether they are safe, efficacious, and cost-effective in various clinical settings.  N. Ref:: 28

 

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[54]

TÍTULO / TITLE:  - Tissue factor and coronary artery disease.

REVISTA / JOURNAL:  - Cardiovasc Res 2002 Feb 1;53(2):313-25.

AUTORES / AUTHORS:  - Moons AH; Levi M; Peters RJ

INSTITUCIÓN / INSTITUTION:  - Department of Cardiology, Academic Medical Center, Room F3-236, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.

RESUMEN / SUMMARY:  - Plaque disruption with superimposed thrombosis is the main cause of acute coronary events such as acute myocardial infarction and unstable angina. Among other factors, tissue factor seems to play an important role determining plaque thrombogenicity. Tissue factor is a potent initiator of the coagulation cascade situated within the vessel wall and is highly exposed to the blood after plaque rupture. Several mediators involved in the process of atherosclerotic plaque formation are capable of inducing tissue factor expression in cells such as monocytes, macrophages and endothelial cells, which under normal conditions do not express tissue factor or to a limited extent only. The increased expression of tissue factor is not limited to the plaque but is also found in circulating monocytes in patients with acute coronary syndromes. In addition, studies have shown an important contribution of tissue factor in the pathogenesis of thrombosis and restenosis after balloon angioplasty. Recent basic studies focus on the therapeutic inhibition of tissue factor. Specific and non-specific inhibitors of tissue factor or the tissue factor/factor VIIa complex have been developed or identified, and have been tested in experimental studies. Clinical studies are currently being initiated. In this review, we present the current knowledge on the role of tissue factor in atherosclerosis, arterial intervention and potential pharmacological approaches, with focus on acute coronary syndromes.  N. Ref:: 162

 

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[55]

- Castellano -

TÍTULO / TITLE:Las inmunofilinas: agentes neuroprotectores y promotores de la regeneracion neural. Immunophilins: neuroprotective agents and promoters of neural regeneration.

REVISTA / JOURNAL:  - Neurologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.stmeditores.com/Revistas/ffasciculo.php?Mw==&MTk=&MjUy 

      ●● Cita: Neurologia: <> 2002 Apr;17(4):200-13.

AUTORES / AUTHORS:  - Udina E; Navarro X

INSTITUCIÓN / INSTITUTION:  - Grupo de Neuroplasticidad y Regeneracion, Departamento de Biologia Celular, Fisiologia e Inmunologia, Universitat Autonoma de Barcelona, Bellaterra, España.

RESUMEN / SUMMARY:  - Immunophilins are a family of proteins mainly known because they act as receptors of the immunosuppressant drugs cyclosporin A (CsA) and FK506. Immunophilins serve several general functions, including regulation of mitochondrial permeability, modulation of ion channels stability and acting as chaperones for a variety of proteins. However, immunophilins are also present at high density in the nervous system. CsA, FK506 and other derivatives inhibit the function of immunophilins and, through bloking or activating several intracellular pathways, it has been shown that they exert neuroprotective effects in different experimental models of ischemia, Parkinson’s disease and excitotoxic insults. Moreover, FK506 also has neuroregenerative effects, by enhancing the axonal regeneration rate after lesions of the peripheral nervous system. The development of new agents that selectively bind to immunophilins opens new interesting perspectives for the therapy of degenerative diseases and injuries of the nervous system.  N. Ref:: 100

 

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[56]

TÍTULO / TITLE:  - Immunoablation followed or not by hematopoietic stem cells as an intense therapy for severe autoimmune diseases. New perspectives, new problems.

REVISTA / JOURNAL:  - Haematologica. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://db.doyma.es/ 

      ●● Cita: Haematologica: <> 2001 Apr;86(4):337-45.

AUTORES / AUTHORS:  - Marmont AM  N. Ref:: 127

 

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[57]

TÍTULO / TITLE:  - mTOR as a target for cancer therapy.

REVISTA / JOURNAL:  - Curr Top Microbiol Immunol 2004;279:339-59.

AUTORES / AUTHORS:  - Houghton PJ; Huang S

INSTITUCIÓN / INSTITUTION:  - Department of Molecular Pharmacology, St. Jude Children’s Research Hospital, 332 N. Lauderdale, Memphis, TN 38105-2794, USA. peter.houghton@stjude.org

RESUMEN / SUMMARY:  - The target of rapamycin, mTOR, acts as a sensor for mitogenic stimuli, such as insulin-like growth factors and cellular nutritional status, regulating cellular growth and division. As many tumors are driven by autocrine or paracrine growth through the type-I insulin-like growth factor receptor, mTOR is potentially an attractive target for molecular-targeted treatment. Further, a rationale for anticipating tumor-selective activity based on transforming events frequently identified in malignant disease is becoming established.  N. Ref:: 73

 

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[58]

TÍTULO / TITLE:  - Serine-threonine protein phosphatase inhibitors: development of potential therapeutic strategies.

REVISTA / JOURNAL:  - J Med Chem 2002 Mar 14;45(6):1151-75.

AUTORES / AUTHORS:  - McCluskey A; Sim AT; Sakoff JA

INSTITUCIÓN / INSTITUTION:  - School of Biological & Chemical Science, Medicinal Chemistry Group, The University of Newcastle, Callaghan, NSW 2308, Australia. amcclusk@mail.newcastle.edu.au  N. Ref:: 329

 

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[59]

TÍTULO / TITLE:  - Inflammatory myopathies: clinical, diagnostic and therapeutic aspects.

REVISTA / JOURNAL:  - Muscle Nerve 2003 Apr;27(4):407-25.

      ●● Enlace al texto completo (gratuito o de pago) 1002/mus.10313

AUTORES / AUTHORS:  - Mastaglia FL; Garlepp MJ; Phillips BA; Zilko PJ

INSTITUCIÓN / INSTITUTION:  - Centre for Neuromuscular and Neurological Disorders, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Australia. flmast@cyllene.uwa.edu.au

RESUMEN / SUMMARY:  - The three major forms of immune-mediated inflammatory myopathy are dermatomyositis (DM), polymyositis (PM), and inclusion-body myositis (IBM). They each have distinctive clinical and histopathologic features that allow the clinician to reach a specific diagnosis in most cases. Magnetic resonance imaging is sometimes helpful, particularly if the diagnosis of IBM is suspected but has not been formally evaluated. Myositis-specific antibodies are not helpful diagnostically but may be of prognostic value; most antibodies have low sensitivity. Muscle biopsy is mandatory to confirm the diagnosis of an inflammatory myopathy and to allow unusual varieties such as eosinophilic, granulomatous, and parasitic myositis, and macrophagic myofasciitis, to be recognized. The treatment of the inflammatory myopathies remains largely empirical and relies upon the use of corticosteroids, immunosuppressive agents, and intravenous immunoglobulin, all of which have nonselective effects on the immune system. Further controlled clinical trials are required to evaluate the relative efficacy of the available therapeutic modalities particularly in combinations, and of newer immunosuppressive agents (mycophenolate mofetil and tacrolimus) and cytokine-based therapies for the treatment of resistant cases of DM, PM, and IBM. Improved understanding of the molecular mechanisms of muscle injury in the inflammatory myopathies should lead to the development of more specific forms of immunotherapy for these conditions.  N. Ref:: 256

 

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[60]

TÍTULO / TITLE:  - Mitochondrial permeability transition in acute neurodegeneration.

REVISTA / JOURNAL:  - Biochimie 2002 Feb-Mar;84(2-3):241-50.

AUTORES / AUTHORS:  - Friberg H; Wieloch T

INSTITUCIÓN / INSTITUTION:  - Laboratory for Experimental Brain Research, Wallenberg Neuroscience Center, BMC A13, 221 84 Lund, Sweden.

RESUMEN / SUMMARY:  - Acute neurodegeneration in man is encountered during and following stroke, transient cardiac arrest, brain trauma, insulin-induced hypoglycemia and status epilepticus. All these severe clinical conditions are characterized by neuronal calcium overload, aberrant cell signaling, generation of free radicals and elevation of cellular free fatty acids, conditions that favor activation of the mitochondrial permeability transition pore (mtPTP). Cyclosporin A (CsA) and its analog N-methyl-valine-4-cyclosporin A (MeValCsA) are potent blockers of the mtPTP and protect against neuronal death following excitotoxicity and oxygen glucose deprivation. Also, CsA and MeValCsA diminish cell death following cerebral ischemia, trauma, and hypoglycemia. Here we present data that strongly imply the mtPT in acute neurodegeneration in vivo. Compounds that readily pass the blood-brain-barrier (BBB) and block the mtPT may be neuroprotective in stroke.  N. Ref:: 100

 

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[61]

TÍTULO / TITLE:  - How should the immunosuppressive regimen be managed in patients with established chronic allograft failure?

REVISTA / JOURNAL:  - Kidney Int Suppl 2002 May;(80):68-72.

AUTORES / AUTHORS:  - Danovitch GM

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, UCLA School of Medicine, USA. gdanovitch@mednet.ucla.edu  N. Ref:: 25

 

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[62]

TÍTULO / TITLE:  - Calcineurin-free protocols with basiliximab induction allow patients included in “old to old” programs achieve standard kidney transplant function.

REVISTA / JOURNAL:  - Transplant Proc 2003 Jun;35(4):1326-7.

AUTORES / AUTHORS:  - Emparan C; Laukotter M; Wolters H; Dame C; Heidenreich S; Senninger N

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Division of Transplantation, Uniklinikum Munster, Munster, Germany. cemparan@teleline.es

RESUMEN / SUMMARY:  - INTRODUCTION: The EuroTransplant “old to old” program establishes that patients older than 60 years can receive offers of organs from donors older than 60 years. The compromised function of these organs makes it a priority to preserve their initial kidney function. HYPOTHESIS: Calcineurin-sparing protocols using anti-IL-2 receptor (IL-2R) antibody induction (Simulect) may benefit initial kidney function in these patients, as assessed by the rates of delayed graft function and of rejection during the first month after transplant. PATIENTS AND METHODS: A cohort of 15 consecutive elderly patients were prospectively compared with 30 cadaveric kidney transplants in younger recipients. Study patients were induced with Simulect (20 mg, 30 minutes before reperfusion and 4 days after transplantation) and steroids, delaying the introduction of CsA until the serum creatinine was below 3 mg/dL. The other cohort of patients were immunosuppressed with tacrolimus (trough 8 to 12), mycophenolats mofetil (MMF, 1 g/d), and an identical taper of steroids. The analysis compared donor and recipient ages, mean cold ischemic time, incidence of initial kidney function (diuresis in the first 24 h) serum creatinine levels, glomerular filtration rate (GFR), number of dialysis sessions, and rejection rate in the two groups. RESULTS: Except for the donor and recipient ages (72 vs 54 in donors, and 67 versus 52 years in recipients), no significant differences were observed between the groups among the rates of acute rejection (6.6% vs 13.2%), delayed graft function (13.2% required dialysis), or infection (6.6%). Within 1 month all 45 grafts showed primary function with equal creatinine levels (mean 1.65). CONCLUSIONS: Calcineurin-free protocols using IL-2 therapy as the initial suppression allow patients in the “old to old” ET program to display equal results to cadaveric kidney transplants with initial treatment with calcineurin antagonists.

 

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[63]

TÍTULO / TITLE:  - Measle virus-infected dendritic cells develop immunosuppressive and cytotoxic activities.

REVISTA / JOURNAL:  - Immunobiology 2001 Dec;204(5):629-38.

AUTORES / AUTHORS:  - Vidalain PO; Azocar O; Rabourdin-Combe C; Servet-Delprat C

INSTITUCIÓN / INSTITUTION:  - Immunobiologie Fondamentale et Clinique, CERVI-INSERM, Lyon, France. servet@cervi-lyon.inserm.fr

RESUMEN / SUMMARY:  - Measle virus (MV) infection induces a transient but profound immunosuppression characterized by a panlymphopenia which occasionally results in opportunistic infections responsible for a high rate of mortality in malnourished children. MV can encounter human dendritic cells (DC) in the respiratory mucosa or in the secondary lymphoid organs. After a brief presentation of DCs, we review progress in understanding the immunobiology of MV-infected DCs that could account for MV-induced immunosuppression. In addition, we develop the newly described TRAIL-mediated cytotoxic function of DCs that is turned on by MV infection, but also by interferons or double-stranded RNA (poly (I:C)). Finally, we propose a model where the measles-associated lymphopenia could be mediated by TRAIL and the measles-induced immunosuppression could be transiently prolonged by Fas-mediated destruction of DCs.  N. Ref:: 38

 

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[64]

TÍTULO / TITLE:  - Antiproliferative prostaglandins and the MRP/GS-X pump role in cancer immunosuppression and insight into new strategies in cancer gene therapy.

REVISTA / JOURNAL:  - Biochem Pharmacol 2001 Oct 1;62(7):811-9.

AUTORES / AUTHORS:  - Homem de Bittencourt PI Jr; Curi R

INSTITUCIÓN / INSTITUTION:  - Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite 500, 90050-170, Porto Alegre, RS, Brazil. pauloivo@vortex.ufrgs.br

RESUMEN / SUMMARY:  - A dramatic complication in late-stage cancer patients is host immunosuppression. Cyclopentenone prostaglandins (CP-PGs) overproduced in cancer may impair the function of the immune system. These agents, if produced at high concentrations, are powerful cytostatic and cytotoxic compounds that may arrest cell proliferation and immune response in cancer. Lymphoid tissues of tumor-bearing animals accumulate large amounts of CP-PGs, whereas the tumor tissue does not. This may be because cancer cells are able to overexpress multidrug resistance-associated protein (Mg(2+)-dependent vanadate-sensitive GS-conjugate export ATPase, MRP/GS-X pump), which extrudes CP-PGs to the extracellular space as glutathione S-conjugates. In contrast, MRP/GS-X pump activity is disproportionately low in lymphocytes. This led us to propose the transfection of lymphocytes with multidrug resistance-associated protein genes (MRP) for further autologous transfusion or direct in vivo delivery to lymphocytes by using adenovirus-retrovirus chimeras in order to restore immune system function in cancer, at least partially. We are currently evaluating MRP-transfected lymphocyte (MTL) therapy, using Walker 256 tumor-bearing rats as a model.  N. Ref:: 49

 

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[65]

TÍTULO / TITLE:  - Cytokine and anti-cytokine therapies for inflammatory bowel disease.

REVISTA / JOURNAL:  - Curr Pharm Des 2003;9(14):1107-13.

AUTORES / AUTHORS:  - Ogata H; Hibi T

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

RESUMEN / SUMMARY:  - Although the pathogenesis of inflammatory bowel disease (IBD) remains elusive, it appears that there is chronic activation of the immune and inflammatory cascade in genetically susceptible individuals. Current disease management guidelines have therefore focused on the use of anti-inflammatory agents, aminosalicylates and corticosteroids. These conventional therapies continue to be a first choice in the management of IBD. Immunomodulators, such as azathioprine, 6-mercaptopurine, methotrexate or cyclosporin, are demonstrating increasing importance against steroid-resistant and steroid-dependent patients. However, some patients are still refractory to these therapies. Recent advances in the understanding of the pathophysiological conditions of IBD have provided new immune system modulators as therapeutic tools. Other immunosuppressive agents including FK506 and thalidomide have expanded the choice of medical therapies available for certain subgroups of patients. Furthermore, biological therapies have begun to assume a prominent role. Studies with chimeric monoclonal anti-TNF-alpha antibody treatment have been reported with dramatic successes. However, observations in larger numbers of treated patients are needed to explicate fully the safety of or risks posed by this agent such as developing lymphoma, or other malignancies. Another anti-inflammatory cytokine-therapy includes anti anti-IL-6R, anti-IL-12 or toxin-conjugated anti IL-7R, recombinant cytokines (IL-10 or IL-11). Given the diversity of proinflammatory products under its control, NF-kappaB may be viewed as a master switch in lymphocytes and macrophages, regulating inflammation and immunity. Although some of them still need more confirmatory studies, those immune therapies will provide new insights into cell-based and gene-based treatment against IBD in near future.  N. Ref:: 46

 

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[66]

TÍTULO / TITLE:  - Introduction and overview: recent advances in the immunotherapy of inflammatory bowel disease.

REVISTA / JOURNAL:  - J Gastroenterol 2003 Mar;38 Suppl 15:36-42.

AUTORES / AUTHORS:  - Hibi T; Inoue N; Ogata H; Naganuma M

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine, Keio University School of Medicine, Center for the Research of Inflammatory Bowel Disease, Keio University School of Medicine, Tokyo, Japan.

RESUMEN / SUMMARY:  - Ulcerative colitis (UC) and Crohn’s disease (CD) comprise a series of inflammatory bowel disease (IBD) resulting from chronic upregulation of the mucosal immune system. Although the pathogenesis of IBD remains elusive, it appears that there is chronic activation of the immune and inflammatory cascade in genetically susceptible individuals. Current disease management guidelines have therefore focused on the use of antiinflammatory agents, aminosalicylates and corticosteroids. However, some patients are still refractory to these therapies. Recent advances in the understanding of the pathophysiological conditions of IBD have provided new immune system modulators as therapeutic tools. Cytapheresis has demonstrated effectiveness against UC and has practical use in Japan. Immunosuppressive agents including cyclosporin A and tacrolimus (FK506) have expanded the choice of medical therapies available for certain subgroups of patients. Furthermore, biological therapies have begun to assume a prominent role. Studies with chimeric monoclonal anti-TNF-alpha antibody treatment of CD have been reported with dramatic success. Another antiinflammatory cytokine therapy includes anti-IL-6 receptor, anti-IL-12, or toxin-conjugated anti-IL-7 receptor. Given the diversity of proinflammatory products under its control, NF-kappa B may be viewed as a master switch in lymphocytes and macrophages, regulating inflammation and immunity. In the murine 2,4,6-trinitrobenzen sulfonic acid (TNBS) colitis model, an antisense oligonucleotide to NF-kappa B p65 ameliorated inflammation even after induction of colitis. Recently, a clinical pilot trial of this agent demonstrated promising results. Accumulating evidence suggests that luminal bacterial flora is a requisite and central factor in the development of IBD. Probiotic therapies such as a nonpathogenic Escherichia coli strain have been well tolerated, but larger clinical trials are needed. In addition, novel therapeutic strategies targeting adhesion molecules and costimulatory molecules, or enhancing tissue repair, are under investigation. Although some still need more confirmatory studies, these immune therapies will provide new insights into cell-based and gene-based treatment against IBD in the near future.  N. Ref:: 36

 

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[67]

TÍTULO / TITLE:  - Potassium channels in T lymphocytes: toxins to therapeutic immunosuppressants.

REVISTA / JOURNAL:  - Toxicon 2001 Sep;39(9):1269-76.

AUTORES / AUTHORS:  - George Chandy K; Cahalan M; Pennington M; Norton RS; Wulff H; Gutman GA

INSTITUCIÓN / INSTITUTION:  - Department of Physiology and Biophysics, University of California Irvine, Room 291, John Irvine Smith Hall, Medical School, Irvine, CA92697, USA. gchandy@uci.edu  N. Ref:: 60

 

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[68]

TÍTULO / TITLE:  - Graft vascular function after transplantation of pancreatic islets.

REVISTA / JOURNAL:  - Diabetologia 2002 Jun;45(6):749-63. Epub 2002 May 15.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00125-002-0827-4

AUTORES / AUTHORS:  - Jansson L; Carlsson PO

INSTITUCIÓN / INSTITUTION:  - Department of Medical Cell Biology, Biomedical Center, Uppsala University, Box 571, 751 23 Uppsala, Sweden. Leif.Jansson@medcellbiol.uu.se

RESUMEN / SUMMARY:  - Endogenous pancreatic islets have a dense glomerular-like angioarchitecture, which ensures an optimal delivery of oxygen and nutrients to the islet cells, provides signals from other cells in the body and disposes secreted hormones. Transplantation of isolated islets means that their vascular connection is interrupted. The islet grafts therefore depend upon endothelial cells and microvessels originating in the implantation organ for derivation of a new vascular system. A re-establishment of islet blood-flow occurs within 7-14 days after transplantation, mainly through vascular sprouting. The newly formed blood vessels acquire the morphological characteristics of those in endogenous islets. In intraportally transplanted islets to the liver, the islets become revascularized almost exclusively from tributaries to the hepatic artery. Exocrine contamination of the transplanted islets could hamper the revascularization process, whereas neither cryopreservation nor immunosuppressive drugs like cyclosporin, prednisolon and RS-61443 have any essential effects on the angiogenesis. Investigators have noticed improvements in islet graft survival and function by means of basic fibroblast growth factor (bFGF), acidic FGF and endothelial cell growth factor exposure of the grafts. The functional properties of transplanted islets are largely unknown, but evidence from experimental islet transplantation suggests that both the blood perfusion and the tissue oxygen tension of the grafted islets are chronically decreased, indicating an insufficient vascular system. In order to achieve optimal condition for survival and function of transplanted beta cells, it is important to ascertain whether impairments in vascular function are present also after clinical islet transplantations as well.  N. Ref:: 181

 

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[69]

TÍTULO / TITLE:  - Targeted cancer therapy and immunosuppression using radiolabeled monoclonal antibodies.

REVISTA / JOURNAL:  - Semin Oncol 2004 Feb;31(1):68-82.

AUTORES / AUTHORS:  - Bethge WA; Sandmaier BM

INSTITUCIÓN / INSTITUTION:  - Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

RESUMEN / SUMMARY:  - Radioimmunotherapy (RIT) as a means to target radiation therapy to tumor cells or to specifically suppress host immunity specifically in the setting of allogeneic transplantation is a promising new strategy in the armory of today’s oncologist. Different approaches of RIT such as injection of a stable radioimmunoconjugate or the use of pretargeting are available. The choice of the radionuclide used for RIT depends on its radiation characteristics with respect to the malignancy or cells targeted. beta-Emitters with their lower energy and longer path length are more suitable for targeting bulky, solid tumors, whereas alpha-emitters with their high linear energy transfer and short path length are better suited to target cells or tumors of the hematologic system. Encouraging results have been obtained using these approaches treating patients with hematologic malignancies. While the results in solid tumors are somewhat less favorable, new strategies for patients with minimal residual disease (MRD), using adjuvant and locoregional treatment, are currently being investigated. In this report, we outline basic principles of RIT, give an overview of available radioimmunoconjugates and their clinical applications with special emphasis on their use in hematologic malignancies, including use in conditioning regimens for stem cell transplantation (SCT).  N. Ref:: 99

 

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[70]

TÍTULO / TITLE:  - TOR inhibitors and cardiac allograft vasculopathy: is inhibition of intimal thickening an adequate surrogate of benefit?

REVISTA / JOURNAL:  - J Heart Lung Transplant 2003 May;22(5):501-4.

AUTORES / AUTHORS:  - Mehra MR; Uber PA

INSTITUCIÓN / INSTITUTION:  - Cardiomyopathy and Heart Transplantation Center, Ochsner Clinic Foundation, New Orleans, Louisiana 70121, USA. mmehra@ochsner.org  N. Ref:: 30

 

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[71]

TÍTULO / TITLE:  - Hepatitis C virus infection and vasculitis: implications of antiviral and immunosuppressive therapies.

REVISTA / JOURNAL:  - Arthritis Rheum 2002 Mar;46(3):585-97.

      ●● Enlace al texto completo (gratuito o de pago) 1002/art.10107 [pii

      ●● Enlace al texto completo (gratuito o de pago) 1002/art.10107

AUTORES / AUTHORS:  - Vassilopoulos D; Calabrese LH

INSTITUCIÓN / INSTITUTION:  - Hippokration General Hospital, Athens University, Athens, Greece.  N. Ref:: 92

 

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[72]

TÍTULO / TITLE:  - Interactions between cyclosporin and lipid-lowering drugs: implications for organ transplant recipients.

REVISTA / JOURNAL:  - Drugs 2003;63(4):367-78.

AUTORES / AUTHORS:  - Asberg A

INSTITUCIÓN / INSTITUTION:  - Laboratory for Renal Physiology, Section of Nephrology, Medical Department, The National Hospital, Oslo, Norway. anderas@klinmed.uio.no

RESUMEN / SUMMARY:  - Dyslipidaemia is more frequent in solid organ transplant recipients than in the general population, primarily as a result of immunosuppressive drug treatment. Both cyclosporin and corticosteroids are associated with dyslipidaemic adverse effects. In order to reduce the overall cardiovascular risk in these patients, lipid-lowering drugs have become widely used, especially HMG-CoA reductase inhibitors (statins). Cyclosporin, as well as most statins (lovastatin, simvastatin, atorvastatin and pravastatin) are metabolised by cytochrome P450 (CYP)3A4, so a bilateral pharmacokinetic interaction between these drugs is theoretically possible. However, results from several studies show that statins do not induce increased systemic exposure of cyclosporin. A small (but not clinically relevant) reduction in systemic exposure of cyclosporin has actually been shown in many studies. Cyclosporin-treated patients on the other hand show several-fold higher systemic exposure of all statins, both those that are metabolised by CYP3A4 and fluvastatin (metabolised by CYP2C9). Therefore, the mechanism for this interaction does not seem to be solely caused by inhibition of CYP3A4 metabolism, but it is probably also a result of inhibition of statin-transport in the liver, at least in part. Other lipid-lowering drugs, such as fibric acid derivatives, bile acid sequestrants, probucol, fish oils and orlistat are also used in solid organ transplant recipients. Most of them do not interact with cyclosporin, but there are reports indicating that both probucol and orlistat may reduce cyclosporin bioavailablility to a clinically relevant degree. There is no information on possible interaction effects of cyclosporin on the pharmacokinetics of lipid-lowering drugs other than statins, but it is not likely that any clinical relevant interference exists with fish oil, orlistat, probucol or bile acid sequestrants.  N. Ref:: 71

 

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[73]

TÍTULO / TITLE:  - Fluorescence polarization detection for affinity capillary electrophoresis.

REVISTA / JOURNAL:  - Electrophoresis 2002 Mar;23(6):903-8.

      ●● Enlace al texto completo (gratuito o de pago) 1002/1522-2683(200203)23:6<903::AID-ELPS903>3.0.CO;2-2 [pii]

AUTORES / AUTHORS:  - Le XC; Wan QH; Lam MT

INSTITUCIÓN / INSTITUTION:  - Environmental Health Sciences Program, Department of Public Health Sciences, Faculty of Medicine, University of Alberta, Edmonton, Alberta T6G 2G3, Canada. xc.le@ualberta.ca

RESUMEN / SUMMARY:  - Affinity capillary electrophoresis (ACE) with laser-induced fluorescence polarization (LIFP) detection is described, with examples of affinity interaction studies. Because fluorescence polarization is sensitive to changes in the rotational motion arising from molecular association or dissociation, ACE-LIFP is capable of providing information on the formation of affinity complexes prior to or during CE separation. Unbound, small fluorescent probes generally have little fluorescence polarization because of rapid rotation of the molecule in solution. When the small fluorescent probe is bound to a larger affinity agent, such as an antibody, the fluorescence polarization (and anisotropy) increases due to slower motion of the much larger complex molecule in the solution. Fluorescence polarization results are obtained by simultaneously measuring fluorescence intensities of vertical and horizontal polarization planes. Applications of CE-LIFP to both strong and weak binding systems are discussed with antibody-antigen and DNA-protein binding as examples. For strong affinity binding, such as between cyclosporine and its antibody, complexes are formed prior to CE-LIFP analysis. For weaker binding, such as between single-stranded DNA and its binding protein, the single-stranded DNA binding protein is added to the CE separation buffer to enhance dynamic formation of affinity complexes. Both fluorescence polarization (and anisotropy) and mobility shift results are complementary and are useful for immunoassays and binding studies.  N. Ref:: 25

 

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[74]

TÍTULO / TITLE:  - Steroid-resistant kidney transplant rejection: diagnosis and treatment.

REVISTA / JOURNAL:  - J Am Soc Nephrol. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.jasn.org/ 

      ●● Cita: Journal of the American Society of Nephrology: <> 2001 Feb;12 Suppl 17:S48-52.

AUTORES / AUTHORS:  - Bock HA

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, Kantonsspital, Aarau, Switzerland. bock@ksa.ch

RESUMEN / SUMMARY:  - Decreases in transplant function may be attributable to a variety of conditions, including prerenal and postrenal failure, cyclosporin A (CsA) toxicity, polyoma nephritis, recurrent glomerulonephritis, and rejection. The diagnosis of rejection should therefore be made on the basis of a transplant biopsy of adequate size, before the initiation of any therapy. Pulse steroid treatment (three to five 0.25- to 1.0-g pulses of methylprednisolone, administered intravenously) is the usual first-line therapy and has a 60 to 70% success rate, although orally administered prednisone (0.25 g) may be just as efficacious. Even if reverted, any rejection should trigger an at least temporary increase in basal immunosuppression, consisting of an increase in CsA or tacrolimus target levels, the addition of steroids or an increase in their dosage, the addition of mycophenolate mofetil, or a switch from CsA to tacrolimus. The addition of rapamycin or its RAD derivative may fulfill the same purpose. Steroid resistance should not be assumed before the fifth day of pulse steroid treatment, although histologic features of vascular rejection may indicate the need for more aggressive treatment earlier. Steroid-resistant rejection is traditionally treated with poly- or monoclonal antilymphocytic antibodies, with success rates of 60 to 70%. Their potential benefit must be carefully balanced against the risks of infection and lymphoma. More recently, mycophenolate mofetil has been successfully used to treat steroid-resistant rejection, but only of the interstitial (cellular) type. Switching from CsA to tacrolimus for treating recurrent or antibody-resistant rejection is successful in approximately 60% of cases. Plasmapheresis and intravenously administered Ig have been used in some desperate cases, with surprising success. Because none of the available drugs has a significantly better profile of therapeutic versus adverse effects, the possible benefits of continued rejection therapy must be continuously balanced with the potential for serious, sometimes fatal, side effects.  N. Ref:: 35

 

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[75]

TÍTULO / TITLE:  - A novel pathway regulating the mammalian target of rapamycin (mTOR) signaling.

REVISTA / JOURNAL:  - Biochem Pharmacol 2002 Oct 1;64(7):1071-7.

AUTORES / AUTHORS:  - Chen J; Fang Y

INSTITUCIÓN / INSTITUTION:  - Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, 601 South Goodwin Avenue, B107, Urbana, IL 61801, USA. jiechen@uiuc.edu

RESUMEN / SUMMARY:  - Originally discovered as an anti-fungal agent, the bacterial macrolide rapamycin is a potent immunosuppressant and a promising anti-cancer drug. In complex with its cellular receptor, the FK506-binding protein (FKBP12), rapamycin binds and inhibits the function of the mammalian target of rapamycin (mTOR). By mediating amino acid sufficiency, mTOR governs signaling to translational regulation and other cellular functions by converging with the phosphatidylinositol 3-kinase (PI3K) pathway on downstream effectors. Whether mTOR receives mitogenic signals in addition to nutrient-sensing has been an unresolved issue, and the mechanism of action of rapamycin remained unknown. Our recent findings have revealed a novel link between mitogenic signals and mTOR via the lipid second messenger phosphatidic acid (PA), and suggested a role for mTOR in the integration of nutrient and mitogen signals. A molecular mechanism for rapamycin inhibition of mTOR signaling is proposed, in which a putative interaction between PA and mTOR is abolished by rapamycin binding. Collective evidence further implicates the regulation of the rapamycin-sensitive signaling circuitry by phospholipase D, and potentially by other upstream regulators such as the conventional protein kinase C, the Rho and ARF families of small G proteins, and calcium ions. As the mTOR pathway has been demonstrated to be an important anti-cancer target, the identification of new components and novel regulatory modes in mTOR signaling will facilitate the future development of diagnostic and therapeutic strategies.  N. Ref:: 67

 

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[76]

TÍTULO / TITLE:  - ATP-binding cassette transporters and calcineurin inhibitors: potential clinical implications.

REVISTA / JOURNAL:  - Transplant Proc 2001 May;33(3):2420-1.

AUTORES / AUTHORS:  - van Gelder T; Klupp J; Sawamoto T; Christians U; Morris RE

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine (T.vG.), University Hospital Rotterdam, Rotterdam, The Netherlands. vangelder@INW1.AZR.NL  N. Ref:: 17

 

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[77]

TÍTULO / TITLE:  - Recent developments in inflammatory bowel disease.

REVISTA / JOURNAL:  - Med Clin North Am 2002 Nov;86(6):1497-523.

AUTORES / AUTHORS:  - Su C; Lichtenstein GR

INSTITUCIÓN / INSTITUTION:  - Division of Gastroenterology, Department of Medicine, Hospital of the University of Pennsylvania, University of Pennsylvania School of Medicine, Third Floor Ravdin Building, 3400 Spruce Street, Philadelphia, PA 19104-4283, USA.

RESUMEN / SUMMARY:  - The evolving medical armamentarium holds promise for more precise and effective therapies for IBD. The experience with anti-TNF therapy, particularly infliximab, illustrates the potential efficacy of therapies targeted at specific mediators or pathways involved in the pathogenesis. Advances in molecular technology have enabled the development of novel and potentially effective targeted therapies. Equally important is the increasing scientific understanding of the pathogenesis of IBD, which will likely improve the ability to stratify disease and to select therapies based on genotypic, immunologic, and phenotypic profiles in the future.  N. Ref:: 191

 

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[78]

- Castellano -

TÍTULO / TITLE:Leucoencefalopatia posterior reversible en un paciente con linfoma no-Hodgkin tras tratamiento con CHOP. Reversible posterior leukoencephalopathy in a patient with non-Hodgkin’s lymphoma after treatment with CHOP.

REVISTA / JOURNAL:  - An Med Interna 2001 Nov;18(11):591-3.

AUTORES / AUTHORS:  - Gimenez-Mesa E; Martinez-Salio A; Porta-Etessam J; Berbel Garcia A; Cedena Romero T; Salama Bendoyan P

INSTITUCIÓN / INSTITUTION:  - Servicio de Neurologia, Hospital Universitario Doce de Octubre, Ctra de Andalucia km 5,400, 28041 Madrid.

RESUMEN / SUMMARY:  - Reversible posterior leukoencephalopathy syndrome is a newly characterised and increasingly recognized clinico-radiologic syndrome. Underlying conditions that reportedly trigger this syndrome include hypertensive encephalopathy, eclampsia, renal failure, and immunosuppressive drug therapy with cyclosporine, tacrolimus and interferon alpha. We describe a 51-year-old woman with non-Hodgkin’s lymphoma treated with conventional CHOP chemotherapy. Eight days after this treatment she developed severe headache, bilateral visual loss and focal seizures with secondary generalization. Neurologic examination showed confusion, cortical blindness, and left hemiparesis with hyperreflexia and sensory loss. A cranial T2-weighted magnetic resonance imaging revealed increased signal intensity in the occipital and frontal lobes in both hemispheres and right parietal lobe. A diagnosis of reversible posterior leukoencephalopathy was made. She presented a favourable outcome with conservative treatment with mannitol and phenytoin. A new cranial scanning showed nearly complete resolution of the abnormalities. To the best of our knowledge, this is the first case of reversible posterior leukoencephalopathy in a patient treated with standard-dose CHOP. In this patient, we confirm the theoretical pathophysiologic mechanisms suggested explaining how these drugs can cause the syndrome.  N. Ref:: 7

 

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[79]

TÍTULO / TITLE:  - FTY720: altered lymphocyte traffic results in allograft protection.

REVISTA / JOURNAL:  - Transplantation 2001 Sep 15;72(5):764-9.

AUTORES / AUTHORS:  - Brinkmann V; Pinschewer DD; Feng L; Chen S

INSTITUCIÓN / INSTITUTION:  - Novartis Pharma AG, Transplantation Research, WSJ-386.1.01, CH-4002 Basel, Switzerland.  N. Ref:: 52

 

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[80]

TÍTULO / TITLE:  - Neonatal toxic shock syndrome-like exanthematous disease (NTED).

REVISTA / JOURNAL:  - Pediatr Int 2003 Apr;45(2):233-7.

AUTORES / AUTHORS:  - Takahashi N

INSTITUCIÓN / INSTITUTION:  - Department of Pediatrics, Jichi Medical School, Tochigi-ken, Tokyo Women’s Medical University, Tokyo, Japan. naoto-t@jichi.ac.jp

RESUMEN / SUMMARY:  - The author and colleagues recently discovered an emerging neonatal infectious disease: neonatal toxic shock syndrome-like exanthematous disease (NTED), which is induced by the superantigen toxic shock syndrome toxin-1 (TSST-1), produced by methicillin-resistant Staphylococcus aureus (MRSA). The massively expanded Vbeta2+ T cells were rapidly deleted in the peripheral blood of patients with NTED. A marked depletion of Vbeta2+ T cells was also observed in the peripheral blood before the expansion of these T cells. Anergy is specifically induced in the TSST-1 reactive T cells of patients with NTED. Rapid recovery from NTED without complications is expected to be related to the induction of immunologic tolerance in neonatal patients. Anti-TSST-1 IgG antibody of maternal origin was found to play a protective role in preventing the development of NTED. The number of hospitals that have experience caring for patients with NTED has increased threefold in the past 5 years. Most MRSA isolates from neonatal intensive care units in Japan were found to be a single clone of coagulase type II and to possess TSST-1 and staphylococcal enterotoxin C genes. The timing and increased incidence of NTED suggest the emergence of a new MRSA clone. By recognizing that TSST-1 can induce NTED, healthcare providers may give increased attention to this disease in neonatal wards.  N. Ref:: 43

 

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[81]

TÍTULO / TITLE:  - Clinical validation studies of Neoral C(2) monitoring: a review.

REVISTA / JOURNAL:  - Transplantation 2002 May 15;73(9 Suppl):S3-11.

AUTORES / AUTHORS:  - Nashan B; Cole E; Levy G; Thervet E

INSTITUCIÓN / INSTITUTION:  - Klinik fur Viszeral und Transplantationschirurgie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.  N. Ref:: 34

 

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[82]

TÍTULO / TITLE:  - Alloimmunity and nonimmunologic risk factors in cardiac allograft vasculopathy.

REVISTA / JOURNAL:  - Eur Heart J 2003 Jul;24(13):1180-8.

AUTORES / AUTHORS:  - Vassalli G; Gallino A; Weis M; von Scheidt W; Kappenberger L; von Segesser LK; Goy JJ

INSTITUCIÓN / INSTITUTION:  - Division of Cardiology, University Hospital, Lausanne, Switzerland. gvassall@hospvd.ch

RESUMEN / SUMMARY:  - Graft vasculopathy is an accelerated form of coronary artery disease that occurs in transplanted hearts. Despite major advances in immunosuppression, the prevalence of the disease has remained substantially unchanged during the last two decades. According to the ‘response to injury’ paradigm, graft vasculopathy is the result of a continuous inflammatory response to tissue injury initiated by both alloantigen-dependent and independent stress responses. Experimental evidence suggests that these responses may become self-sustaining, as allograft re-transplantation into the donor strain at a later stage fails to prevent disease progression. Histological evidence of endothelitis and arteritis, in association with intima fibrosis and atherosclerosis, reflects the central role of alloimmunity and inflammation in the development of arterial lesions. Experimental results in gene-targeted mouse models indicate that cellular and humoral immune responses are both involved in the pathogenesis of graft vasculopathy. Circulating antibodies against donor endothelium are found in a significant number of patients, but their pathogenic role is still controversial. Alloantigen-independent factors include donor-transmitted coronary artery disease, surgical trauma, ischaemia-reperfusion injury, viral infections, hyperlipidaemia, hypertension, and glucose intolerance. Recent therapeutic advances include the use of novel immunosuppressive agents such as sirolimus (rapamycin), HMG-CoA reductase inhibitors, calcium channel blockers, and angiotensin converting enzyme inhibitors. Optimal treatment of cardiovascular risk factors remains of paramount importance.  N. Ref:: 100

 

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[83]

TÍTULO / TITLE:  - Basiliximab: a review of its use as induction therapy in renal transplantation.

REVISTA / JOURNAL:  - Drugs 2003;63(24):2803-35.

AUTORES / AUTHORS:  - Chapman TM; Keating GM

INSTITUCIÓN / INSTITUTION:  - Adis International Limited, Auckland, New Zealand. demail@adis.co.nz

RESUMEN / SUMMARY:  - Basiliximab (Simulect), a chimeric (human/murine) monoclonal antibody, is indicated for the prevention of acute organ rejection in adult and paediatric renal transplant recipients in combination with other immunosuppressive agents.Basiliximab significantly reduced acute rejection compared with placebo in renal transplant recipients receiving dual- (cyclosporin microemulsion and corticosteroids) or triple-immunotherapy (azathioprine- or mycophenolate mofetil-based); graft and patient survival rates at 12 months were similar. Significantly more basiliximab than placebo recipients were free from the combined endpoint of death, graft loss or acute rejection 3 years, but not 5 years, after transplantation.The incidence of adverse events was similar in basiliximab and placebo recipients, with no increase in the incidence of infection, including cytomegalovirus (CMV) infection. Malignancies or post-transplant lymphoproliferative disorders after treatment with basiliximab were rare, with a similar incidence to that seen with placebo at 12 months or 5 years post-transplantation. Rare cases of hypersensitivity reactions to basiliximab have been reported.The efficacy of basiliximab was similar to that of equine antithymocyte globulin (ATG) and daclizumab, and similar to or greater than that of muromonab CD3. Basiliximab was as effective as rabbit antithymocyte globulin (RATG) in patients at relatively low risk of acute rejection, but less effective in high-risk patients. Numerically or significantly fewer patients receiving basiliximab experienced adverse events considered to be related to the study drug than ATG or RATG recipients. The incidence of infection, including CMV infection, was similar with basiliximab and ATG or RATG.Basiliximab plus baseline immunosuppression resulted in no significant differences in acute rejection rates compared with baseline immunosuppression with or without ATG or antilymphocyte globulin in retrospective analyses conducted for small numbers of paediatric patients. Limited data from paediatric renal transplant recipients suggest a similar tolerability profile to that in adults. Basiliximab appears to allow the withdrawal of corticosteroids or the use of corticosteroid-free or calcineurin inhibitor-sparing regimens in renal transplant recipients.Basiliximab did not increase the overall costs of therapy in pharmacoeconomic studies.CONCLUSION: Basiliximab reduces acute rejection without increasing the incidence of adverse events, including infection and malignancy, in renal transplant recipients when combined with standard dual- or triple-immunotherapy. The overall incidence of death, graft loss or acute rejection was significantly reduced at 3 years; there was no significant difference for this endpoint 5 years after transplantation. Malignancy was not increased at 5 years. The overall efficacy, tolerability, ease of administration and cost effectiveness of basiliximab make it an attractive option for the prophylaxis of acute renal transplant rejection.  N. Ref:: 85

 

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[84]

TÍTULO / TITLE:  - Cyclosporine-associated hyperkalemia: report of four allogeneic blood stem-cell transplant cases.

REVISTA / JOURNAL:  - Transplantation 2003 Apr 15;75(7):1069-72.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000057241.69355.59

AUTORES / AUTHORS:  - Caliskan Y; Kalayoglu-Besisik S; Sargin D; Ecder T

INSTITUCIÓN / INSTITUTION:  - Bone Marrow Transplantation Unit, Department of Internal Medicine, Division of Hematology, Istanbul School of Medicine, CAPA 34 390, Istanbul, Turkey.

RESUMEN / SUMMARY:  - BACKGROUND: Nephrotoxicity is a well-known effect of cyclosporine (CsA) that causes a reduction in glomerular filtration rate through vasoconstriction of the afferent glomerular arterioles and may result in acute renal failure. Isolated CsA-induced hyperkalemia occurring through different mechanisms is also common. However, there are only a few “case reports” addressing this phenomenon in allogeneic bone marrow transplantation patients. In this report, we propose mechanisms and methods of managing CsA-associated hyperkalemia in allogeneic transplantation. METHODS: We report on four allogeneic blood stem- cell transplant cases and a review of the literature. RESULTS: Four adult leukemia patients underwent allogeneic peripheral blood stem cell transplantation and received CsA as a part of their graft-versus-host disease prophylaxis. The patients developed hyperkalemia, despite adequate kidney function. CsA seemed to be the only pharmaceutical agent to which this electrolyte abnormality could be attributed. Renal tubule dysfunction and secondary hypoaldosteronism seemed to be the reasons for CsA-associated hyperkalemia. CONCLUSION: This report of four cases demonstrates that CsA should be considered among the possible causes of hyperkalemia in bone marrow transplantation. There may be a need for urgent intervention depending on the severity of hyperkalemia. Monitoring of blood CsA level and dose adjustment are important for the prevention of this complication.  N. Ref:: 22

 

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[85]

TÍTULO / TITLE:  - Pharmacokinetics of tacrolimus-based combination therapies.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2003 May;18 Suppl 1:i12-5.

AUTORES / AUTHORS:  - Undre NA

INSTITUCIÓN / INSTITUTION:  - Fujisawa GmbH, Neumarkter Str. 61, D-81673 Munich, Germany. nas.undre@fujisawa.de

RESUMEN / SUMMARY:  - This paper reviews the pharmacokinetics of tacrolimus, with special reference to its combination with adjunctive immunosuppressants. Oral bioavailability of tacrolimus, which is variable between patients, averages approximately 25%. This is largely due to extrahepatic metabolism of tacrolimus in the gastrointestinal epithelium. Nevertheless, intra-patient variability is low, as evidenced by the small number of dose changes required to maintain patients within the recommended tacrolimus target levels. Tacrolimus is distributed extensively in the body with most partitioned outside the blood compartment. Concentrations of tacrolimus in blood are used as a surrogate marker of clinically relevant concentration of the drug at the site(s) of action. Convenient whole-blood sampling within a +/-2-h window around 12 h post-dose (C(min)) is highly predictive of systemic exposure to tacrolimus and is thus used to optimise therapy. Sampling at other time-points offers no advantage over C(min) monitoring. The interactions of tacrolimus with other immunosuppressive agents are well characterized. After cessation of concomitant corticosteroid treatment, exposure to tacrolimus increases by approximately 25%. In contrast, there is no pharmacokinetic interaction between mycophenolate mofetil (MMF) and tacrolimus. Therefore, systemic exposure to the active metabolite of MMF, mycophenolic acid, is higher with MMF-tacrolimus combination than with MMF-cyclosporin combination. Therefore, 1 g/day MMF may be an adequate maintenance dose in tacrolimus-based regimens. Co-administration of tacrolimus and sirolimus, while having no effect on exposure to sirolimus, results in reduced exposure to tacrolimus at sirolimus doses of 2 mg/day and above. In conclusion, tacrolimus levels should be monitored when sirolimus is co-administered at doses >2 mg/day and after cessation of corticosteroid treatment.  N. Ref:: 13

 

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[86]

- Castellano -

TÍTULO / TITLE:La enfermedad linfoproliferativa difusa postrasplante renal y su relacion con el virus Epstein-Barr. Experiencia de un centro. Diffuse lymphoproliferative disease after renal transplantation and its relation with Epstein-Barr virus. Experience at one center.

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2002;22(5):463-9.

AUTORES / AUTHORS:  - Franco A; Jimenez L; Aranda I; Alvarez L; Gonzalez M; Rocamora N; Olivares J

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia Hospital General Alicante Maestro Alonso, 109 03010 Alicante. franco_ant@gva.es

RESUMEN / SUMMARY:  - Post-transplant lymphoproliferative disorders (PTLD) are a group of heterogeneous lymphoid proliferations in chronic immunosuppressed recipients which appear to be related to Epstein Barr Virus (EBV). Receptor EBV seronegativity, use of antilymphocyte antibodies and CMV disease have been identified as risk factors that may tigger development of PTLD. We have studied the incidence of PTLD and its relationship with EBV in 588 adult renal transplant recipients who were transplanted in our hospital from 1988 to 2001. We have also evaluated the diagnostic and therapeutic methods used, the risk factors and outcome of the patients who developed PTLD. We identified 8 recipients (4 males and 4 females), range from 18 to 67 years (mean age 45.6 years) with a median time between grafting and PTLD of 4.1 years (0.1-7 years), who developed PTLD (1.3%). Only 1 patient received OKT3 and had CMV disease, two of them (25%) had been treated with hight doses of prednisolone, another was EBV seronegative, but the rest of them (50%) had no risk factors. Two patients were diagnosed at autopsy, the diagnosis of 5 was based on the histology of biopsy and the last one by CT scans of chest-abdomen and cytology. The presence of EBV in the lymphoproliferative cells was assessed in 5 out of the 7 studied patients (71.4%). The outcome of our recipients was poor. Five out of 8 patients died shortly after diagnosis as a direct consecuence of PTLD and another of an infectious complication of the treatment (75%). The 2 patients alive started dialysis and 1 of them died 2 years later of a non-related cause. In conclusion, PTLD is a relatively frequent disease with a poor prognosis in renal transplant patients. It seems to have a close relationship with EBV and can develop in the absence of the classical risk factors.  N. Ref:: 18

 

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[87]

TÍTULO / TITLE:  - Primary intestinal posttransplant T-cell lymphoma.

REVISTA / JOURNAL:  - Transplantation 2003 Jun 27;75(12):2131-2.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000060253.54333.F3

AUTORES / AUTHORS:  - Michael J; Greenstein S; Schechner R; Tellis V; Vasovic LV; Ratech H; Glicklich D

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA.

RESUMEN / SUMMARY:  - There have been only five reported cases of primary posttransplant T-cell lymphoma. We report the first case associated with the use of sirolimus (Rapamycin, Wyeth-Ayerst, Philadelphia, PA). The patient, receiving prednisone, cyclosporine, and sirolimus treatment, developed ascites, diarrhea, and weight loss 7 months after his second renal transplant. Tissue obtained at laparotomy established the diagnosis of primary T-cell lymphoma. Latent membrane protein-1 for Epstein-Barr virus was negative, but in-site hybridization test for Epstein-Barr-encoded RNA was positive. Despite aggressive chemotherapy, the patient died 8 months posttransplant. This is the sixth reported case of primary intestinal posttransplant T-cell lymphoma, but it is the first case associated with the use of sirolimus. The incidence of posttransplant lymphoproliferative disease in patients receiving sirolimus should be studied.  N. Ref:: 6

 

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[88]

- Castellano -

TÍTULO / TITLE:Alteraciones del metabolismo oseo tras el trasplante renal. Bone metabolism alterations after kidney transplantation.

REVISTA / JOURNAL:  - Nefrologia. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.aulamedica.es/nefrologia/ 

      ●● Cita: Nefrologia: <> 2003;23 Suppl 2:122-6.

AUTORES / AUTHORS:  - Torres A; Garcia S; Barrios Y; Hernandez D; Lorenzo V

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia, Unidad de Investigacion, Hospital Universitario de Canarias, Instituto Reina Sofia de Investigacion. atorres@ull.es

RESUMEN / SUMMARY:  - Early after renal transplantation (RT) a rapid decrease in bone mineral density at the lumbar spine, femoral neck, and femoral shaft has been documented. In addition, an appreciable proportion of patients still remain losing bone late after RT. As a consequence, RT patients are at a high risk of bone fractures as compared to general population. Most fractures involve appendicular skeleton, particularly the feet and ankles, and the diabetic patient is at increased risk of fractures. Thus, early institution of preventive measures and treatment of established osteoporosis are central. The major cause of post-transplantation bone loss is corticosteroid treatment, and this should be used at the lower dose compatible with graft survival. Preexisting hyperparathyroidism also affects the early cancellous bone loss at the spine, and post-transplantation bone loss reflects variable individual susceptibility, resembling the polygenic determination of bone mineral density in general. Clinical trials have demonstrated that bisphosphonates or vitamin D plus calcium supplementation, prevent post-transplantation bone loss during the first 6-12 months. However, their role in preventing bone fractures has not been proven. Finally, recommendations for management, prevention and treatment, are summarized.  N. Ref:: 24

 

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[89]

TÍTULO / TITLE:  - Efficacy and toxicity of a protocol using sirolimus, tacrolimus and daclizumab in a nonhuman primate renal allotransplant model.

REVISTA / JOURNAL:  - Am J Transplant 2002 Apr;2(4):381-5.

AUTORES / AUTHORS:  - Montgomery SP; Mog SR; Xu H; Tadaki DK; Hirshberg B; Berning JD; Leconte J; Harlan DM; Hale D; Kirk AD

INSTITUCIÓN / INSTITUTION:  - NIDDK/Navy Transplantation and Autoimmunity Branch, Naval Medical Research Center, Bethesda, Maryland 20892, USA.

RESUMEN / SUMMARY:  - A regimen combining sirolimus, tacrolimus, and daclizumab has recently been shown to provide adequate immunosuppression for allogeneic islet transplantation in humans, but remains unproven for primarily vascularized allografts. We evaluated this regimen for renal allograft transplantation in mismatched nonhuman primates. Dosages of sirolimus and tacrolimus were adjusted for trough levels of 10-15 ng/mL and 4-6 ng/mL, respectively. Treated monkeys (n = 5) had significantly prolonged allograft survival, with a mean survival of 36 days vs. 7 days in untreated controls (n = 6, p = 0.008). Four of five treated animals, but none of the controls, developed fibrinoid vascular necrosis of the small intestine. A review of gut histology from animals on other immunosuppressive protocols performed by our laboratory suggested that these lesions were a result of sirolimus exposure. In summary, this regimen prolongs the survival of vascularized renal allografts, but is limited by profound GI toxicity in rhesus macaques.

 

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[90]

TÍTULO / TITLE:  - Treatment responses of childhood aplastic anaemia with chromosomal aberrations at diagnosis.

REVISTA / JOURNAL:  - Br J Haematol 2002 Jul;118(1):313-9.

AUTORES / AUTHORS:  - Ohga S; Ohara A; Hibi S; Kojima S; Bessho F; Tsuchiya S; Ohshima Y; Yoshida N; Kashii Y; Nishimura S; Kawakami K; Nishikawa K; Tsukimoto I

INSTITUCIÓN / INSTITUTION:  - Aplastic Anaemia Committee of the Japanese Society of Paediatric Haematology, Tokyo, Japan. ohgas@pediatr.med.kyushu-u.ac.jp

RESUMEN / SUMMARY:  - The clinical outcome of childhood aplastic anaemia (AA) with aberrant cytogenetic clones at diagnosis was surveyed. Among 198 children with newly diagnosed AA registered with the AA Committee of the Japanese Society of Paediatric Hematology between 1994 and 1998, cytogenetic studies of bone marrow (BM) cells were completed in 159 patients. Apart from one Robertsonian translocation, seven patients (4.4%) showed clonal chromosomal abnormalities in hypoplastic BM without myelodysplastic features. The patients included six girls and one boy with a median age of 11 years (range 5-14 years). Six patients had del(6), del(5), del(13), del(20), or -7, and one showed add(9). Four patients responded to the first immunosuppressive therapy (IST: cyclosporin A plus anti-thymocyte globulin) and one obtained a spontaneous remission. Cytogenetic abnormalities remained in two patients with an IST response. On the other hand, two patients showed no IST response. One did not respond to repeat IST and died of acute graft-versus-host disease after an unrelated-BM transplant. Another obtained a complete response after a successful BM transplant. No haematological findings at diagnosis predicted the treatment response. No significant morphological changes developed during the course of the illness. A literature review revealed that half of 24 AA patients with chromosomal abnormalities responded to the first IST, and that +6 was the sole predictable marker for IST unresponsiveness. These results suggest that IST can be applied as the initial therapy for AA with cytogenetic abnormalities in the absence of completely matched donors.  N. Ref:: 32

 

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[91]

TÍTULO / TITLE:  - Acute necrotizing gastritis by Escherichia coli in a severely neutropenic patient.

REVISTA / JOURNAL:  - Haematologica. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://db.doyma.es/ 

      ●● Cita: Haematologica: <> 2002 Jan;87(1):ELT01.

AUTORES / AUTHORS:  - Martinez-Chamorro C; Martinez E; Gil-Fernandez JJ; Escudero A; Acevedo A; Fernandez-Ranada JM

INSTITUCIÓN / INSTITUTION:  - Hematology Department, Clinica Ruber, C/Juan Bravo, 49 28006-Madrid, España. m-chamorro@navegalia.com  N. Ref:: 6

 

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[92]

TÍTULO / TITLE:  - Lichen amyloidosus associated with Kimura’s disease: successful treatment with cyclosporine.

REVISTA / JOURNAL:  - Dermatology 2002;204(2):133-5.

AUTORES / AUTHORS:  - Teraki Y; Katsuta M; Shiohara T

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Kyorin University School of Medicine, Tokyo, Japan. teraki@kyorin-u.ac.jp

RESUMEN / SUMMARY:  - We describe here a case of a 33-year-old man who had lichen amyloidosus associated with Kimura’s disease. In this case, treatment with cyclosporine dramatically improved the lesions of both Kimura’s disease and lichen amyloidosus. Although Kimura’s disease and lichen amyloidosus are both rare distinct entities, to our knowledge, 11 cases of association of Kimura’s disease and lichen amyloidosus have been described previously.  N. Ref:: 13

 

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[93]

TÍTULO / TITLE:  - Inhibitors of mammalian target of rapamycin as novel antitumor agents: from bench to clinic.

REVISTA / JOURNAL:  - Curr Opin Investig Drugs 2002 Feb;3(2):295-304.

AUTORES / AUTHORS:  - Huang S; Houghton PJ

INSTITUCIÓN / INSTITUTION:  - Department of Molecular Pharmacology, St Jude Children’s Research Hospital, Memphis, TN 38105-2794, USA.

RESUMEN / SUMMARY:  - Rapamycin and its derivatives, CCI-779 and RAD-001, inhibit the mammalian target of rapamycin (mTOR), downregulating translation of specific mRNAs required for cell cycle progression from G1 to S phase. Preclinically, mTOR inhibitors potently suppress growth and proliferation of numerous tumor cell lines in culture or when grown in mice as xenografts. CCI-779 and RAD-001 are being developed as antitumor drugs and are undergoing clinical trials. Clinically, CCI-779 has shown evidence of antitumor activity but induced relatively mild side effects in patients. Here we discuss potential antitumor mechanisms and resistance mechanisms of mTOR inhibitors, and summarize the current status of these compounds as novel antitumor agents.  N. Ref:: 90

 

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[94]

TÍTULO / TITLE:  - Pathophysiology of traumatic brain edema: current concepts.

REVISTA / JOURNAL:  - Acta Neurochir Suppl 2003;86:7-10.

AUTORES / AUTHORS:  - Marmarou A

INSTITUCIÓN / INSTITUTION:  - Division of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298-0508, USA. marmarou@hsc.vcu.edu

RESUMEN / SUMMARY:  - The generally held concept during the past several decades is that traumatic brain edema is predominately vasogenic emanating from the blood vessels subsequent to blood brain barrier compromise. Much of the experimental data has focused on cryogenic injury models where there clearly is a necrotic lesion surrounded by leaking vessels. However, in closed head injury where brain swelling remains a critical problem, the classification of the type of edema that develops is less clear. Most importantly, studies in the clinical setting have ruled out vascular engorgement as one potential mechanism and these studies have shown that edema and not blood volume is the culprit responsible for brain swelling. We have put forth the notion that traumatic brain edema is a combination of vasogenic and cellular with the cellular component predominating. This article provides an update of our current progress toward supporting this hypothesis and includes an update on the role of aquaporins in traumatic brain edema.  N. Ref:: 43

 

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[95]

TÍTULO / TITLE:  - Regulation of translation via TOR signaling: insights from Drosophila melanogaster.

REVISTA / JOURNAL:  - J Nutr. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://www.nutrition.org/ 

      ●● Cita: Journal of Nutrition: <> 2001 Nov;131(11):2988S-93S.

AUTORES / AUTHORS:  - Miron M; Sonenberg N

INSTITUCIÓN / INSTITUTION:  - Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada.

RESUMEN / SUMMARY:  - The target of rapamycin (TOR) proteins are large protein kinases evolutionarily conserved from yeast to human. A large body of evidence demonstrates that TOR proteins function in a nutrient-sensing checkpoint whose role is to restrict growth under conditions of low nutrient availability. Under such conditions, TOR blocks the transmission of growth-promoting signals from extracellular stimuli. Recent data obtained by genetic studies in the fruit fly Drosophila melanogaster demonstrate the importance of both insulin-like signaling and TOR signaling in promoting growth. Importantly, these studies identified a major downstream target of TOR and insulin-like signaling as the translational machinery.  N. Ref:: 63

 

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[96]

TÍTULO / TITLE:  - Treatment of severe acute graft-versus-host disease with anti-thymocyte globulin.

REVISTA / JOURNAL:  - Clin Transplant 2001 Jun;15(3):147-53.

AUTORES / AUTHORS:  - Remberger M; Aschan J; Barkholt L; Tollemar J; Ringden O

INSTITUCIÓN / INSTITUTION:  - Department of Clinical Immunology and Centre for Allogeneic Stem Cell Transplantation, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden. mats.remberger@impi.ki.se

RESUMEN / SUMMARY:  - Severe acute graft-versus-host disease (GVHD) is one of the major complications after haematopoietic stem-cell transplantation (HSCT). Treatment of severe GVHD is difficult and the condition is often fatal. One proposed method of improving the therapy is to include anti-thymocyte globulin (ATG). Here, we will report our results in 29 patients using ATG as part of treatment for severe steroid-resistant acute GVHD. Four patients suffered from grade II, 13 from grade III and 12 from grade IV GVHD. Median time to grade II GVHD was 24 d (range 7-91 d) and to grade III was 29 d (range 8-55 d) after HSCT. Five different ATG preparations were used, rabbit ATG (R-ATG), BMA 031, OKT3, ATG-Fresenius and Thymoglobuline. All patients had skin involvement, 26 also had gut involvement and 25 had liver involvement. The rate of response to treatment was best in skin involvement (72%), while liver and gut involvement showed lower response rates (38%). Eleven patients survived more than 90 d, 7 of them developed chronic GVHD, 1 developed mild GVHD, 1 developed moderate GVHD and 5 developed severe GVHD. Survival at 100 d was 37% and at 1 yr it was 12%. Most patients died of GVHD, with virus or fungal infections as contributing causes of death. To conclude, treatment of severe acute GVHD is difficult and ATG, in our hands, adds nothing to conventional pharmacological treatment.  N. Ref:: 48

 

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[97]

TÍTULO / TITLE:  - Mitochondrial involvement in the point of no return in neuronal apoptosis.

REVISTA / JOURNAL:  - Biochimie 2002 Feb-Mar;84(2-3):223-31.

AUTORES / AUTHORS:  - Chang LK; Putcha GV; Deshmukh M; Johnson EM Jr

INSTITUCIÓN / INSTITUTION:  - Department of Neurology, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8103, St. Louis, MO 63110-1031, USA.

RESUMEN / SUMMARY:  - Programmed cell death (PCD) contributes to development, maintenance, and pathology in various tissues, including the nervous system. Many molecular, biochemical, and genetic events occur within cells undergoing PCD. Some of these events are incompatible with long-term cell survival because they have irreversible, catastrophic consequences. The onset of such changes marks the point of no return, a decisive regulatory event termed ‘the commitment-to-die.’ In this review, we discuss events that underlie the commitment-to-die in nerve growth factor-deprivation-induced death of sympathetic neurons. Findings in this model system implicate the mitochondrion as an important site of regulation for the commitment-to-die in the presence or absence of caspase inhibition.  N. Ref:: 57

 

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[98]

TÍTULO / TITLE:  - Hyperlipidemia and cardiovascular disease after organ transplantation.

REVISTA / JOURNAL:  - Transplantation 2001 Sep 27;72(6 Suppl):S13-5.

AUTORES / AUTHORS:  - Massy ZA

INSTITUCIÓN / INSTITUTION:  - INSERM U507, Necker Hospital, Paris, France. massy@necker.fr

RESUMEN / SUMMARY:  - Hyperlipidemia, a frequent and persistent complication after solid organ transplantation, contributes to cardiovascular morbidity and mortality and may influence the development of allograft vasculopathy. The pathogenesis of posttransplantation hyperlipidemia is not fully understood, although several epidemiological factors are strongly implicated including age, weight, pretransplantation lipid levels, and immunosuppressive therapy. Management strategies to reduce hyperlipidemia and modify cardiovascular risk include dietary restrictions and the use of lipid-lowering agents. The selective use of immunosuppressants, such as tacrolimus, that have neutral or fewer adverse effects on lipid metabolism may also provide a useful option. A combination of lipid-lowering therapies and optimization of immunosuppressive regimens compatible with prolonged allograft survival is probably necessary to significantly reduce posttransplantation hyperlipidemia and its potentially harmful consequences.  N. Ref:: 44

 

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[99]

TÍTULO / TITLE:  - Paraneoplastic pemphigus in association with a retroperitoneal Castleman’s disease presenting with a lichen planus pemphigoides-like eruption. A case report and review of literature.

REVISTA / JOURNAL:  - Br J Dermatol 2001 Feb;144(2):372-6.

AUTORES / AUTHORS:  - Hsiao CJ; Hsu MM; Lee JY; Chen WC; Hsieh WC

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, National Cheng-Kung University Hospital, 138 Sheng-Li Road, Tainan, Taiwan 704.

RESUMEN / SUMMARY:  - A 50-year-old man presented with severe mucosal erosions of the lips, oral cavity and perianal area, a lichen planus-like eruption on the trunk and extremities and scaly plaques of the palms and soles. The clinical impression was of Stevens—Johnson syndrome, or paraneoplastic pemphigus (PNP). Histopathology revealed vacuolar interface and lichenoid dermatitis with dyskeratosis and suprabasal acantholytic vesiculation. Direct immunofluorescence showed deposition of IgG in the intercellular space and linear deposition of C3 along the basal membrane zone. Indirect immunofluorescence revealed circulating IgG with intercellular staining of the epithelium of rat urinary bladder. Western blotting demonstrated bands of 250- and 230-kDa antigens. The clinical, histological and immunological features were consistent with the lichen planus pemphigoides variant of PNP. A retroperitoneal hyaline-vascular Castleman’s disease was detected and excised. The skin lesions worsened initially after tumour resection but improved gradually, leaving extensive melanosis after cyclosporin and mycophenolate mofetil treatment.  N. Ref:: 21

 

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[100]

TÍTULO / TITLE:  - First human double hand transplantation: efficacy of a conventional immunosuppressive protocol.

REVISTA / JOURNAL:  - Clin Transplant 2003 Oct;17(5):455-60.

AUTORES / AUTHORS:  - Petruzzo P; Revillard JP; Kanitakis J; Lanzetta M; Hakim NS; Lefrancois N; Owen E; Dubernard JM

INSTITUCIÓN / INSTITUTION:  - Service de Chirurgie de Transplantation, Hopital Edouard Herriot, Lyon, France.

RESUMEN / SUMMARY:  - Based on the results achieved in single human hand transplantations, we decided to perform the first double hand transplantation with a conventional immunosuppressive protocol in a patient with a high potential for functional recovery. Two years after transplantation the efficacy and the safety of this immunosuppressive protocol are evaluated. The recipient was a 33-yr-old man suffering from a traumatic amputation of both hands in 1996. Five HLA-A, -B, and -DR mismatches were present with the donor; T and B cell cross-match was negative. Immunosuppressive protocol included tacrolimus, prednisone, mycophenolate mofetil and, for induction, antithymocyte globulins and then anti CD25 monoclonal antibody. Reconstitution of lymphocyte populations proceeded normally. Neither anti-HLA antibodies nor chimerism in peripheral blood were detected. Two episodes of acute rejection characterized by maculopapular lesions occurred on days 53 and 82 after transplantation. Skin biopsies revealed a dermal lymphocytic infiltrate. Both episodes were completely and rapidly reversed by topical clobetasol and increased systemic corticosteroid therapy. The only side-effects related to treatment were reversible serum sickness and hyperglycemia. No infectious complications and malignancies occurred. No signs of graft-versus-host disease have been detected. This case of double hand transplantation shows that conventional immunosuppression is effective and safe to ensure survival and functional recovery of the grafted limb.

 

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[101]

TÍTULO / TITLE:  - Mechanisms and consequences of arterial hypertension after renal transplantation.

REVISTA / JOURNAL:  - Transplantation 2001 Sep 27;72(6 Suppl):S9-12.

AUTORES / AUTHORS:  - Koomans HA; Ligtenberg G

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology and Hypertension, University Hospital Utrecht, The Netherlands. h.a.koomans@digd.azu.nl

RESUMEN / SUMMARY:  - The high incidence of hypertension after renal transplantation contributes to the risk of cardiovascular morbidity and mortality in renal transplant recipients. Although cyclosporine has been influential in the improvement of transplant outcome, it has emerged as a major cause of hypertension after organ transplantation. The underlying pathophysiological mechanisms of cyclosporine-induced hypertension include enhanced sympathetic nervous system activity, renal vasoconstriction, and sodium/water retention. Hypertension is also significantly associated with reduced graft survival and thereby requires aggressive treatment intervention. Calcium channel blockers may offer some advantages over angiotensin-converting enzyme inhibitors for the treatment of hypertension in stable renal transplant recipients. Nevertheless, selection of the most appropriate antihypertensive agent should take into account the possibility of pharmacokinetic interactions with immunosuppressive agents. There is evidence to suggest that the use of tacrolimus-based immunosuppression induces less hypertension compared with cyclosporine. Not only do patients receiving tacrolimus tend to require less antihypertensive therapy, but converting patients from cyclosporine to tacrolimus has been shown to result in significant reductions in blood pressure. Thus, tacrolimus may be associated with an improved cardiovascular risk profile in renal transplant recipients.  N. Ref:: 26

 

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[102]

TÍTULO / TITLE:  - Assessing cardiovascular risk profile of immunosuppressive agents.

REVISTA / JOURNAL:  - Transplantation 2001 Dec 27;72(12 Suppl):S81-8.

AUTORES / AUTHORS:  - Jardine A  N. Ref:: 57

 

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[103]

TÍTULO / TITLE:  - T-cell receptor-derived peptides in immunoregulation and therapy of retrovirally induced immunosuppression.

REVISTA / JOURNAL:  - Crit Rev Immunol 2001;21(1-3):57-74.

AUTORES / AUTHORS:  - Marchalonis JJ; Robey IF; Edmundson AB; Sepulveda RT; Watson RR

INSTITUCIÓN / INSTITUTION:  - Microbiology and Immunology, College of Medicine, University of Arizona, Tucson 85724, USA. dianah@u.arizona.edu

RESUMEN / SUMMARY:  - Retrovirally infected humans and mice showed progressive acquired immunodeficiency accompanied by the production of elevated levels of autoantibodies directed against T-cell receptor variable-domain epitopes. Epitope mapping analyses indicated that a major determinant recognized was defined by a 16-mer peptide containing the entire CDR1 segment and part of the FR2 region of human Vbeta8, and that both species showed reactivity to the same sequence. Either prophylactic or therapeutic administration of this peptide to retrovirus-infected C57/BL/6 mice normalized the balance of T(H)1- and T(H)2-type helper activity and restored the resistance to infection by the opportunistic parasite Cryptosporidium. Administration of the peptide did not generate significantly increased levels of autoantibody, but had a profound effect on T-cell activity as well as other aspects of inflammation, including NK-cell activity. A 16-mer derived from the Jbeta sequence showed similar functional effects on T cells from retrovirus-infected mice. Direct binding of the VbetaCDR1 peptide to recombinant TCR Valpha/Vbeta constructs, as well as to IgM natural autoantibodies, suggests that the cell surface receptor for the peptide is the alpha/beta TCR on T cells and surface IgM in B cells. The Vbeta CDR1 peptide stimulated division of murine splenocytes in vitro, stimulated the production of the T(H)1 cytokine IL-2, and synergized with the T-cell mitogen concanavalin A in proliferation and IL-2 production. These studies indicate that administration of peptides derived from T-cell receptor variable domains to animals immunosuppressed as a result of retroviral infection has a profound immunomodulatory effect enhancing overall T-cell functional capacity, particularly with respect to the cytokine production characteristic of T(H)1-type cells. Our studies are interpreted in the context of other recent investigations of immunomodulatory peptides.  N. Ref:: 69

 

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[104]

TÍTULO / TITLE:  - Current and future applications of immunological attenuation via pegylation of cells and tissue.

REVISTA / JOURNAL:  - BioDrugs 2001;15(12):833-47.

AUTORES / AUTHORS:  - Chen AM; Scott MD

INSTITUCIÓN / INSTITUTION:  - Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.

RESUMEN / SUMMARY:  - Prevention of immunological rejection of transplanted tissues is of crucial importance in transplantation medicine. Current procedures primarily use pharmacological agents such as cyclosporin, which, while effective, must be typically administered for the life of the individual. Furthermore, the drug-induced global immunosuppression of the patient predisposes the individual to infection and enhances their risk of developing certain forms of cancer. Hence, additional methods are needed to both enhance tissue engraftment and diminish the adverse effects of current immunosuppressive therapy. Studies from blood transfusion (i.e. a specialised form of cellular transplantation) suggest that covalent modification of cells and tissues with methoxypoly(ethylene glycol) [mPEG] can significantly diminish rejection episodes and may further enhance the induction of tolerance to donor tissues. The mechanisms underlying mPEG-mediated immunocamouflage are the loss of antigen recognition, impaired cell-cell interaction, and an inability of endogenous antibodies (e.g. immunoglobulin G) to effectively recognise and bind foreign epitopes. As a consequence of the global camouflage imparted by mPEG, the weak co-stimulation of alloreactive T cells may subsequently induce apoptosis, thus leading to tolerance. Initial studies on the transplantation of pegylated isogeneic rat pancreatic islets demonstrates that mPEG-derivatisation does not impair in vivo cellular signalling and function. Thus, in contrast to the pharmacological inhibition of the recipient’s immune response, the mPEG-mediated immunocamouflage directly addresses the inherent antigenicity and immunogenicity of the donor tissue itself while leaving the recipient a fully competent immune system.  N. Ref:: 43

 

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[105]

TÍTULO / TITLE:  - Controlling the incidence of infection and malignancy by modifying immunosuppression.

REVISTA / JOURNAL:  - Transplantation 2001 Dec 27;72(12 Suppl):S89-93.

AUTORES / AUTHORS:  - Soulillou JP; Giral M

RESUMEN / SUMMARY:  - Long-term outcomes in renal transplantation have improved over the years but are still a matter of concern. Because patients typically require lifelong immunosuppression, the risks of cancer and infection associated with immunosuppressive agents continue to demand attention. Physicians strive endlessly to find the right balance between the level of immunosuppression required to prevent rejection and the level that will minimize dose-dependent side effects. Data presented in this paper suggest that some renal transplant recipients might have more than necessary immunosuppression during maintenance therapy and that reducing the immunosuppressant dose can decrease cancer incidence, without worsening long-term patient or allograft survival. Additionally, data were examined suggesting that immunosuppressive agents might be associated with different risks for cancer, specifically, the potential advantage of reduced cancer risk for sirolimus and sirolimus derivatives in comparison with standard immunosuppressive agents. Although promising, these preliminary results are from preclinical studies, and further study is warranted.  N. Ref:: 42

 

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[106]

TÍTULO / TITLE:  - P-glycoprotein in acute myeloid leukaemia: therapeutic implications of its association with both a multidrug-resistant and an apoptosis-resistant phenotype.

REVISTA / JOURNAL:  - Leuk Lymphoma 2002 Jun;43(6):1221-8.

AUTORES / AUTHORS:  - Pallis M; Turzanski J; Higashi Y; Russell N

INSTITUCIÓN / INSTITUTION:  - Academic Haematology, Nottingham City Hospital, Nottingham, UK. monica.pallis@nottingham.ac.uk

RESUMEN / SUMMARY:  - P-glycoprotein (Pgp) expression is an independent prognostic factor for response to remission-induction chemotherapy in acute myeloblastic leukaemia, particularly in the elderly. There are several potential agents for modulating Pgp-mediated multi-drug resistance, such as cyclosporin A and PSC833, which are currently being evaluated in clinical trials. An alternative therapeutic strategy is to increase the use of drugs which are unaffected by Pgp. However, in this review, we explain why this may be more difficult than it appears. Evidence from in vitro studies of primary AML blasts supports the commonly held supposition that chemoresistance may be linked to apoptosis-resistance. We have found that Pgp has a drug-independent role in the inhibition of in vitro apoptosis in AML blasts. Modulation of cytokine efflux, signalling lipids and intracellular pH have all been suggested as ways by which Pgp may affect cellular resistance to apoptosis; these are discussed in this review. For a chemosensitising agent to be successful, it may be more important for it to enhance apoptosis than to increase drug uptake.  N. Ref:: 95

 

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[107]

TÍTULO / TITLE:  - The potential of antibody-based immunosuppressive agents for corneal transplantation.

REVISTA / JOURNAL:  - Immunol Cell Biol 2003 Apr;81(2):93-105.

AUTORES / AUTHORS:  - Thiel MA; Coster DJ; Williams KA

INSTITUCIÓN / INSTITUTION:  - Department of Ophthalmology, Flinders University of South Australia, Adelaide, Australia.

RESUMEN / SUMMARY:  - Corneal transplantation is a sight-restorative procedure but its success is limited by irreversible graft rejection, which accounts for up to 50 per cent of failures. The normal eye is an immune-privileged site. Multiple mechanisms maintain ocular privilege, including the blood-eye barrier, the lack of blood vessels and lymphatics in the normal cornea, the relative paucity of mature antigen-presenting cells in the central cornea, the presence of immunomodulatory factors in ocular fluids, and the constitutive expressive of CD95L (Fas ligand) within the eye. However, privilege can be eroded by the sequelae of inflammation and neovascularization. Corneal graft rejection in humans is currently suppressed with topical glucocorticosteroids, which are moderately effective. Systemically administered immunosuppressive therapy is of limited efficacy and may be accompanied by unacceptable morbidity. Alternative therapies are needed to improve outcomes. Corneal graft rejection is primarily a cell-mediated response controlled by the CD4+ T cell, and thus CD4 and costimulatory molecule blockade are appealing targets for new therapeutic interventions. A number of monoclonal antibodies have shown promise as immunosuppressants to prolong corneal graft survival in experimental animal models, and may eventually prove to be useful adjuncts to corticosteroids.  N. Ref:: 205

 

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[108]

TÍTULO / TITLE:  - A retrospective review of sirolimus (Rapamune) therapy in orthotopic liver transplant recipients diagnosed with chronic rejection.

REVISTA / JOURNAL:  - Liver Transpl 2003 May;9(5):477-83.

      ●● Enlace al texto completo (gratuito o de pago) 1053/jlts.2003.50119

AUTORES / AUTHORS:  - Neff GW; Montalbano M; Slapak-Green G; Berney T; Bejarano PA; Joshi A; Icardi M; Nery J; Seigo N; Levi D; Weppler D; Pappas P; Ruiz J; Schiff ER; Tzakis AG

INSTITUCIÓN / INSTITUTION:  - University of Miami, Department of Medicine, Miami, FL 33136, USA. gneff@med.miami.edu

RESUMEN / SUMMARY:  - Treatment options are limited for orthotopic liver transplant (OLT) recipients suffering from chronic rejection (CR). We performed a retrospective review of OLT recipients diagnosed with CR and treated with sirolimus. The medical records of all OLT recipients treated with sirolimus between October, 1998 and October, 2000 were retrospectively reviewed. The diagnosis of CR was made by both clinical and histologic criteria: bile duct to hepatic artery ratio less than 0.7, histologic activity index, hepatic arterial wall thickening, and chronic elevation of liver chemistries. Two groups were defined in regard to sirolimus response: sirolimus responders (SR) and sirolimus nonresponders (SNR). Response to treatment was granted only when patients were found to have resolution of abnormal liver transaminases and an improvement in hepatic artery to bile duct ratio. Serum collections for liver chemistries were collected on days 1, 30, 60, and 90. Liver biopsies were reviewed in blinded fashion from day 1 and at least 180 days on therapy by double-blinded pathologists. Sirolimus-related complications were recorded and include drug toxicity, anemia with and without treatment, hospitalizations, infections, immunosuppression complications, lipid profile disorders, edema, muscle aches, and gastrointestinal complaints. Twenty-one patients were diagnosed with CR. The SR group included 13 of 21, and 8 of 21 were in the SNR group. Anemia was diagnosed in 12 of 21 patients: SR, 7 of 13; SNR, 5 of 8; with 5 patients requiring red blood cell transfusions (2 SR, 3 SNR). Recombinant erythropoietin was started in 5 of 21 patients. Sirolimus serum levels were found to be greater than 20 ng/dL in 12 patients. Sirolimus was discontinued in 9 patients,

 

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[109]

TÍTULO / TITLE:  - Effective prophylactic protocol in delayed hypersensitivity to contrast media: report of a case involving lymphocyte transformation studies with different compounds.

REVISTA / JOURNAL:  - Radiology. Acceso gratuito al texto completo a partir de los 2 años de la publicación;  - http://radiology.rsnajnls.org/ 

      ●● Cita: Radiology: <> 2002 Nov;225(2):466-70.

AUTORES / AUTHORS:  - Romano A; Artesani MC; Andriolo M; Viola M; Pettinato R; Vecchioli-Scaldazza A

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine and Geriatrics, Universita’ Cattolica del Sacro Cuore, Allergy Unit, Complesso Integrato Columbus, Via G. Moscati 31, 00168 Rome, Italy. columbus.allerg@linet.it

RESUMEN / SUMMARY:  - A patient with maculopapular reactions to iopamidol needed to undergo angiography for a cerebral arteriovenous malformation. In vivo and in vitro tests were performed with ionic and nonionic contrast media, including iopamidol and iobitridol. All results were positive, demonstrating delayed hypersensitivity. The patient received 6-alpha-methylprednisolone and cyclosporine 1 week before and 2 weeks after four angiograms were obtained with the use of iobitridol, which was well tolerated.

 

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[110]

TÍTULO / TITLE:  - A pilot protocol of a calcineurin-inhibitor free regimen for kidney transplant recipients of marginal donor kidneys or with delayed graft function.

REVISTA / JOURNAL:  - Clin Transplant 2003;17 Suppl 9:31-4.

AUTORES / AUTHORS:  - Shaffer D; Langone A; Nylander WA; Goral S; Kizilisik AT; Helderman JH

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA. david.schaffer@vanderbilt.edu

RESUMEN / SUMMARY:  - The worsening shortage of cadaver donor kidneys has prompted use of expanded or marginal donor kidneys (MDK), i.e. older age or donor history of hypertension or diabetes. MDK may be especially susceptible to calcineurin-inhibitor (CI) mediated vasoconstriction and nephrotoxicity. Similarly, early use of CI in patients with delayed graft function may prolong ischaemic injury. We developed a CI-free protocol of antibody induction, sirolimus, mycophenolate mofetil, and prednisone in recipients with MDK or DGF. METHODS: Adult renal transplant recipients who received MDK or had DGF were treated with a CI-free protocol consisting of antibody induction (basiliximab or thymoglobulin), sirolimus, mycophenolate mofetil, and prednisone. Serial biopsies were performed for persistent DGF. Patients were followed prospectively with the primary endpoints being patient and graft survival, biopsy-proven acute rejection, and sirolimus-related toxicity. RESULTS: Nineteen recipients were treated. Mean follow-up was 294 days. Actuarial 6- and 12-month patient survival was 100% and 100% and graft survival was 93% and 93%, respectively. The only graft loss was due to primary non-function (PNF). The incidence of AR was 16%. Mean serum creatinine at last follow-up was 1.6 mg/dL. Sirolimus-related toxicity included lymphocele (1), wound infection (2), thrombocytopenia (1). and interstitial pneumonitis (1). CONCLUSION: A CI-free protocol with antibody induction and sirolimus results in low rates of AR and PNF and excellent early patient and graft survival in patients with MDK and DGF. CI-free protocols may allow expansion of the kidney donor pool by encouraging utilization of MDK at high risk for DGF or CI-mediated nephrotoxicity.

 

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[111]

TÍTULO / TITLE:  - Potential role of immune modulation in the effective long-term control of HIV-1 infection.

REVISTA / JOURNAL:  - J Biol Regul Homeost Agents 2002 Jan-Mar;16(1):83-90.

AUTORES / AUTHORS:  - Rizzardi GP; Lazzarin A; Pantaleo G

INSTITUCIÓN / INSTITUTION:  - MOLMED, Milan, Italy. paolo.rizzardi@molmed.it

RESUMEN / SUMMARY:  - Recent advances in HIV-1 pathogenesis, and in defining virological and immunological responses to highly active antiretroviral therapy (HAART), along with the identification of the numerous drawbacks of HAART, have clearly demonstrated that the eradication of the virus is not a feasible therapeutic goal, and that there is an urgent need to develop other approaches to fight HIV-1 infection. Novel therapeutic approaches of immune modulation have recently been evaluated in pilot clinical trials. First, treating primary HIV-1 infection with cyclosporin A (CsA) coupled with HAART to target massive immune activation extends the benefits achieved with HAART during primary HIV-1 infection and might contribute to the establishment of a more favourable immunological set-point affecting the ultimate pattern and rate of disease progression. Second, treating chronic HIV-1 infection in patients with long-term suppression of virus replication induced by HAART, with the addition of mycophenolate mofetil (MMF) reduces the pool of activated CD4+ T lymphocytes able to support productive HIV-1 infection, and might have an indirect impact on the pool of resting, latently infected CD4+ T cells, contributing to its depletion in vivo. The important question is clearly whether these results will have an impact on the clinical management of patients with HIV-1 infection, determining the precise therapeutic function of drugs like CsA and MMF, thus investigating the effects of these drugs on residual viral replication and the decay of the latent reservoir, on long-term immunological benefit, and, ultimately, on clinical benefit.  N. Ref:: 95

 

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[112]

TÍTULO / TITLE:  - Calcineurin and hypertrophic heart disease: novel insights and remaining questions.

REVISTA / JOURNAL:  - Cardiovasc Res 2002 Mar;53(4):806-21.

AUTORES / AUTHORS:  - Bueno OF; van Rooij E; Molkentin JD; Doevendans PA; De Windt LJ

INSTITUCIÓN / INSTITUTION:  - Division of Molecular Cardiovascular Biology, Department of Pediatrics, Children’s Hospital Medical Center, Cincinnati OH, USA.

RESUMEN / SUMMARY:  - In the past 2 years, an emerging body of research has focused on a novel transcriptional pathway involved in the cardiac hypertrophic response. Ever since its introduction, the significance of the calcineurin-NFAT module has been subject of controversy. The aim of this review is to provide both an update on the current status of knowledge and discuss the remaining issues regarding the involvement of calcineurin in hypertrophic heart disease. To this end, the molecular biology of calcineurin and its direct downstream transcriptional effector NFAT are discussed in the context of the genetic studies that established the existence of this signaling paradigm in the heart. The pharmacological mode-of-action and specificity of the calcineurin inhibitors cyclosporine A (CsA) and FK506 is discussed, as well as their inherent limitations to study the biology of calcineurin. A critical interpretation is given on studies aimed at analyzing the role of calcineurin in cardiac hypertrophy using systemic immunosuppression. To eliminate the controversy surrounding CsA/FK506 usage, recent studies employed genetic inhibitory strategies for calcineurin, which confirm the pivotal role for this signal transduction pathway in the ventricular hypertrophy response. Finally, unresolved issues concerning the role of calcineurin in cardiac pathobiology are discussed based upon the information available, including its controversial role in cardiomyocyte viability, the reciprocal relationship between myocyte Ca(2+) homeostasis and calcineurin activity and the relative importance of calcineurin in relation to other hypertrophic signaling cascades.  N. Ref:: 124

 

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[113]

TÍTULO / TITLE:  - Potential therapeutic interventions to avoid or treat chronic allograft dysfunction.

REVISTA / JOURNAL:  - Transplantation 2001 Jun 15;71(11 Suppl):SS52-7.

AUTORES / AUTHORS:  - Kahan BD

INSTITUCIÓN / INSTITUTION:  - University of Texas Medical School Houston, United States.

RESUMEN / SUMMARY:  - Despite the significant improvements that have occurred since the introduction of CsA, long-term renal allograft survival continues to be an area of concern. Management strategies that involve the use of sirolimus offer some promise. A number of observations suggest that sirolimus may have the ability to reduce the rates or slow the progression of chronic nephropathy. First, sirolimus has been shown to inhibit growth-factor-driven proliferation of endothelial and smooth muscle cells in vitro (55, 56). Sirolimus also disrupts signal transduction by a variety of other cytokines such as EGF and PDGE This is significant because cytokine- and growth-factor-stimulated proliferation of endothelial cells, smooth muscle cells, parenchymal cells, and fibroblasts appears to underlie the development of chronic nephropathy (see Fellstrom, this supplement). Second, sirolimus has been demonstrated in various animal models to inhibit the arterial intimal thickening that typically follows alloimmune or mechanical injury (56-60; see Morris, this supplement). This transplant vasculopathy is a prominent feature in chronic rejection of other organ transplants. Moreover, at least 1 published study has suggested that sirolimus may be able to stabilize and possibly reverse chronic graft vascular disease (61). However, the relative doses of sirolimus used in these animal studies have been higher than those used in humans, so the relationship of these effects to the clinical setting needs to be further studied to define the relevance of these findings. Third, sirolimus, used in combination with CsA, reduces the incidence of acute rejection episodes in humans, one of the most significant predictors of shortened renal allograft survival (62, 63). Thus, an effect of sirolimus to reduce acute rejection episodes or delay their onset is expected to reduce renal allograft loss. Furthermore, clinical trials suggest that sirolimus treatment may allow dose reductions of CsA or a delay in inception of CsA therapy, which might reduce the acute and chronic nephrotoxicity associated with CsA and other CNIs. Since nephrotoxicity may promote or aggravate renal injury and appears to be common in chronic nephropathy (see Fellstrom and Paul, this supplement), reduced exposure to CNIs may translate into reduced rates of chronic renal allograft dysfunction. There are no currently effective therapies for chronic nephropathy, which is a common cause of late renal allograft loss. Preliminary evidence suggests that sirolimus may eventually prove useful as prophylaxis of or treatment for chronic nephropathy. Thus, sirolimus has come to be regarded as the foundation for maintenance immunosuppressive regimens.  N. Ref:: 63

 

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[114]

TÍTULO / TITLE:  - Old and new tools to dissect calcineurin’s role in pressure-overload cardiac hypertrophy.

REVISTA / JOURNAL:  - Cardiovasc Res 2002 Feb 1;53(2):294-303.

AUTORES / AUTHORS:  - Zhang W

INSTITUCIÓN / INSTITUTION:  - Department of Internal Medicine/Hypertension Division, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-8586, USA. wzhang@mednet.swmed.edu

RESUMEN / SUMMARY:  - In the last several years, a number of experiments have implicated a pivotal role of the calcium/calmodulin-calcineurin dependent pathway as a final common signaling mechanism by which diverse hypertrophic stimuli converge to mediate hypertrophic responses in cardiomyocytes. Calcineurin inhibitors, i.e. cyclosporine A (CsA) and FK506, can interrupt the pathway, thereby preventing cardiac hypertrophy. The data that convincingly support this novel hypothesis were derived either from in vitro studies in cultured cardiomyocytes or from in vivo studies in transgenic mice. However, when the hypothesis was tested in clinically relevant animal models of cardiac hypertrophy, controversial results and conclusions emerged. In conventional models of cardiac hypertrophy, two questions remain to be answered: (1) whether calcineurin is activated in hypertrophied cardiac muscle, and (2) whether calcineurin inhibitors prevent cardiac hypertrophy. In addition, clinical observations have revealed that calcineurin inhibitors appear to exert pro-hypertrophic effects in organ transplant recipients. The controversies suggest that current calcineurin inhibitors are blunt tools for testing the hypothesis in pressure-overload hypertrophy in vivo, because there are so many confounding effects that are associated with systemic administration of the drugs. As such, new genetic approaches may overcome some of the problems associated with pharmacological inhibitors. This invited review will focus on the controversies surrounding the ability of calcineurin inhibition to prevent conventional (pressure-overload) cardiac hypertrophy and the new genetic approaches to address the question.  N. Ref:: 93

 

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[115]

TÍTULO / TITLE:  - Extragonadal seminoma after renal transplantation and immunosuppression; treatment in the presence of renal dysfunction: a case report and literature review.

REVISTA / JOURNAL:  - Med Oncol 2001;18(3):221-5.

AUTORES / AUTHORS:  - Kosmas C; Tsavaris NB; Vadiaka M; Chiras T; Boletis J; Kostakis A

INSTITUCIÓN / INSTITUTION:  - Department of Pathophysiology, Athens University School of Medicine, Laikon General Hospital, Greece. ckosm@ath.forthnet.gr

RESUMEN / SUMMARY:  - A 37-yr-old man who had undergone renal transplantation for end-stage renal failure presented with a large right pelvic mass obstructing the transplanted kidney. Initially, this was diagnosed as an anaplastic tumor while he had been on immunosuppressive treatment for kidney allograft rejection after transplantation. Despite difficulties of classic histopathology to reveal the origin of his tumor, FISH analysis revealed the presence of chromosome 12p abnormalities, strongly indicative of a germ-cell tumor-more likely seminoma-with extragonadal presentation. Because of renal dysfunction, he was treated with carboplatin (dose adjusted according to renal clearance) and etoposide, and when he experienced a rather atypical progression with bone metastases, he was treated with single-agent paclitaxel, and died almost 13 mo after initial presentation. The case adds further to the existing small list of seminoma/GCTs developing in transplant recipients, points to the unusual presentation patterns and diagnostic histopathology challenges, and presents the difficulty in therapeutic options, as a result of frequent renal dysfunction and intercurrent immunosuppressive therapy. All of these issues together with an extensive literature review are discussed in detail.

 

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[116]

TÍTULO / TITLE:  - Drug-eluting stents: potential applications for peripheral arterial occlusive disease.

REVISTA / JOURNAL:  - J Vasc Interv Radiol 2003 Mar;14(3):291-301.

AUTORES / AUTHORS:  - Duda SH; Poerner TC; Wiesinger B; Rundback JH; Tepe G; Wiskirchen J; Haase KK

INSTITUCIÓN / INSTITUTION:  - Department of Diagnostic Radiology, University of Tuebingen, Germany. stephan.duda@med.uni-tuebingen.de

RESUMEN / SUMMARY:  - Many different approaches have been evaluated to prevent restenosis in stents after vascular implantation. Currently, drug-eluting stents are extremely promising in suppressing neointimal hyperplasia. Various animal studies and randomized trials in humans have shown excellent results in terms of safety and efficacy during intermediate-term follow-up. This article will give an overview of experimental and clinical data of the different agents in published and ongoing trials.  N. Ref:: 87

 

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[117]

TÍTULO / TITLE:  - The role of T lymphocytes in the pathogenesis of asthma.

REVISTA / JOURNAL:  - J Allergy Clin Immunol 2003 Mar;111(3):450-63; quiz 464.

AUTORES / AUTHORS:  - Larche M; Robinson DS; Kay AB

INSTITUCIÓN / INSTITUTION:  - Department of Allergy and Clinical Immunology, Faculty of Medicine, Imperial College London, National Heart and Lung Institute, London, United Kingdom.

RESUMEN / SUMMARY:  - There is considerable evidence to support a role for T cells in asthma, particularly the involvement of T(H)2 cells both in atopic allergic asthma and in nonatopic and occupational asthma. There might also be a minor contribution from T©2 CD8+ T cells. Several T(H)2 cytokines have the potential to modulate airway inflammation, particularly IL-13, which induces airway hyperresponsiveness independently of IgE and eosinophilia in animal models. The identification of transcription factors controlling T(H)1 and T(H)2 development further support the T(H)2 hypothesis because GATA3 is overexpressed and T-bet is underexpressed in the asthmatic airway. Specific T cell directed immunotherapy might allow induction, modulation, or both of T-cell responses, and elucidation of the mechanisms of regulatory T cells might allow further optimization of immunotherapy. Recent advances in our understanding of dendritic cell function in directing T-cell responses might uncover further therapeutic targets. The efficacy of cyclosporin A and anti-CD4 treatment in patients with chronic severe asthma argues for continued T-cell involvement, but whether remodeling contributes to pathology inaccessible to anti-inflammatory treatment or T-cell immunotherapy will be an important future question.  N. Ref:: 145

 

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[118]

TÍTULO / TITLE:  - Immunophilins in nervous system degeneration and regeneration.

REVISTA / JOURNAL:  - Curr Top Med Chem 2003;3(12):1376-82.

AUTORES / AUTHORS:  - Avramut M; Achim CL

INSTITUCIÓN / INSTITUTION:  - Department of Pathology, School of Medicine, University of Pittsburgh, S-433 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15213, USA. avramut@pitt.edu

RESUMEN / SUMMARY:  - Immunophilins are receptors for immunosuppressive drugs like cyclosporin A, FK506, rapamycin and their non- immunosuppressive analogs, which are collectively referred to as “immunophilin ligands” (IPL). Cyclosporin A binds to a class of IP called cyclophilins, whereas the receptors for FK506 and rapamycin belong to the family of FK506- binding proteins (FKBP). The latter are designated according to their molecular weight: FKBP12, 25, 52 etc. FKBP levels in the rat brain are up to 50 times higher than in the immune system. FKBP12 is associated with IP3 and ryanodine receptors present on the endoplasmic reticulum and plays a role in stabilizing calcium release. It has also been proposed to be a modulator of the TGFbeta receptor activity. Crush injury of facial or sciatic nerves in rat leads to markedly increased FKBP12 levels in the respective nerve nuclei and this increase is related to nerve regeneration. Cyclophilin A protects cells from death following expression of mutant Cu/ Zn superoxide dismutase, which is associated with familial amyotrophic lateral sclerosis. Our recent studies show that FKBP12 and FKBP52 are expressed in the human nervous system, especially in the substantia nigra- deep gray matter axis. In neurodegenerative diseases, FKBP12 levels increase in neurons situated in areas of pathology. This IP colocalizes with synaptophysin and alpha- synuclein, suggesting that it may become a novel marker of pathology. Immunophilins participate in axonal transport, synaptic vesicle assembly and may play a role in neuroprotection against abnormal protein aggregation, suggesting a potential avenue of therapeutic interventions.  N. Ref:: 62

 

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[119]

TÍTULO / TITLE:  - Rapamycin in combination with cyclosporine or tacrolimus in liver, pancreas, and kidney transplantation.

REVISTA / JOURNAL:  - Transplant Proc 2003 May;35(3 Suppl):201S-208S.

AUTORES / AUTHORS:  - MacDonald AS

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Dalhousie University, Halifax, Nova Scotia, Canada. Allan.macdonald@dal.ca

RESUMEN / SUMMARY:  - A 10-year experience with the immunosuppressive drug rapamycin that begins in the laboratory then extends through multicentre trials in combination with cyclosporine in kidney transplant recipients, exploration of its use as a single agent and in combination with tacrolimus, and its potential in nonrenal organs is described. Rapamycin is a potent inhibitor of endothelial injury in rat aortic allografts. When added to full-dose cyclosporine it achieves low rejection rates, but it augments the nephrotoxicity and hyperlipidemia of cyclosporine. On the other hand, it allows discontinuation of calcineurin inhibitors in stable kidney and liver patients suffering from nephrotoxicity late posttransplant. At least in Caucasian patients, discontinuation of cyclosporine is possible as early as 3 months post-kidney transplant. In combination with low-dose tacrolimus, exceptionally low rates of rejection were seen in recipients of kidney, pancreas, and liver recipients with preservation of excellent renal function. These pilot studies have been confirmed in several single-centre and, more recently, multicentre trials in kidney and pancreas transplantation. The side-effect profile of hyperlipidemia, lymphocoeles, delayed wound healing, and possible liver effects are coming into focus, and ways of minimizing these problems being introduced. The lessons learned include the need for early adequate blood levels, the lack of correlation between dose and drug exposure, and the potency that allows marked dose reductions in calcineurin inhibitors and steroids.  N. Ref:: 36

 

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[120]

TÍTULO / TITLE:  - Cardiac allograft vasculopathy: current concepts and treatment.

REVISTA / JOURNAL:  - Transpl Int 2003 Jun;16(6):367-75. Epub 2003 May 17.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00147-003-0580-8

AUTORES / AUTHORS:  - Waller J; Brook NR; Nicholson ML

INSTITUCIÓN / INSTITUTION:  - Division of Transplant Surgery, Professorial Unit, Leicester General Hospital, Leicester, Leicestershire, UK. julian@waller720.fsnet.co.uk

RESUMEN / SUMMARY:  - Cardiac allograft vasculopathy (CAV) remains the leading limiting factor of patient and graft survival after the first post-operative year. The pathogenesis involves both immunological and non-immunological factors. Here, we present recent advances and discuss potential preventative and treatment regimens. A review of the current literature of heart transplantation, detailing molecular mechanisms, pharmacological risk factors and novel immunosuppression regimens was performed. Recent findings demonstrate the pivotal role of the endothelium, resulting in release of pro-fibrotic cytokines, recruitment of circulating leucocytes, proliferation of vascular smooth muscle cells, and deposition of extracellular matrix proteins (ECMs). The role of HMG-CoA reductase inhibitors and anti-hypertensives remains controversial, but there is increasing evidence advocating their prophylactic use. We can conclude that novel immunosuppressive agents such as rapamycin, mycophenolate mofetil and FTY-720 are experimental immunosuppressive agents that are undergoing evaluation in clinical trials. The prophylactic use of statins and anti-hypertensive drugs needs to be defined but needs to suggest potential strategies to prolong cardiac allograft survival.  N. Ref:: 102

 

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[121]

TÍTULO / TITLE:  - St John’s Wort supplements endanger the success of organ transplantation.

REVISTA / JOURNAL:  - Arch Surg 2002 Mar;137(3):316-9.

AUTORES / AUTHORS:  - Ernst E

INSTITUCIÓN / INSTITUTION:  - Department of Complementary Medicine, School of Sport and Health Sciences, University of Exeter, 25 Victoria Park Rd, Exeter EX2 4NT, England. E.Ernst@ex.ac.uk

RESUMEN / SUMMARY:  - HYPOTHESIS: St John’s wort is one of the most popular herbal medicines, and health care professionals often are unaware that their patients take such supplements. St John’s wort causes a decrease in cyclosporine levels, thus endangering the success of organ transplantations. DESIGN: Systematic review. METHODS: Five independent computerized literature searches were conducted to identify all reports of such interactions. Data were extracted and are summarized in narrative form. RESULTS: Eleven case reports and 2 case series were located. In most instances, causality between St John’s wort and the clinical or biochemical result is well established. The mechanism of interaction between St John’s wort and cyclosporine has been recently elucidated and involves both P-glycoprotein and cytochrome P 450 3A4 expression. Collectively these data leave little doubt that St John’s wort interacts with cyclosporine, causing a decrease of cyclosporine blood levels and leading in several cases to transplant rejection. CONCLUSIONS: St John’s wort can endanger the success of organ transplantations. Adequate information may be the best way to avoid future incidences.  N. Ref:: 33

 

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[122]

TÍTULO / TITLE:  - Sirolimus and mycophenolate mofetil for calcineurin-free immunosuppression in renal transplant recipients.

REVISTA / JOURNAL:  - Am J Kidney Dis 2001 Oct;38(4 Suppl 2):S16-21.

AUTORES / AUTHORS:  - Pescovitz MD; Govani M

INSTITUCIÓN / INSTITUTION:  - Departments of Surgery, Microbiology/Immunology, and Medicine, Indiana University, Indianapolis, IN 46202, USA. mpescov@iupui.edu

RESUMEN / SUMMARY:  - Calcineurin inhibitors, such as cyclosporine and tacrolimus, have been available for almost 20 years. Although these drugs are highly effective and represent the mainstay of transplant immunosuppression, they are associated with acute and chronic nephrotoxicity. Acute nephrotoxicity, which occurs in the early period after transplantation, leads to a higher rate of dialysis, and chronic nephrotoxicity may eventually result in graft loss. Acute and chronic nephrotoxicity is becoming more common as the use of marginal kidneys for transplantation increases. Two recently available immunosuppressive agents, mycophenolate mofetil and sirolimus (rapamycin), have no nephrotoxicity. The use of these drugs in combination with other agents has led to the development of new paradigms of immunosuppressive therapy. This paper reviews the results of clinical trials that have investigated these new approaches to immunosuppression in renal transplant recipients.  N. Ref:: 9

 

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[123]

TÍTULO / TITLE:  - Engineered CD3 antibodies for immunosuppression.

REVISTA / JOURNAL:  - Clin Exp Immunol 2003 Sep;133(3):307-9.

AUTORES / AUTHORS:  - Renders L; Valerius T  N. Ref:: 30

 

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[124]

TÍTULO / TITLE:  - St John’s wort (Hypericum perforatum): drug interactions and clinical outcomes.

REVISTA / JOURNAL:  - Br J Clin Pharmacol 2002 Oct;54(4):349-56.

AUTORES / AUTHORS:  - Henderson L; Yue QY; Bergquist C; Gerden B; Arlett P

INSTITUCIÓN / INSTITUTION:  - Pharmacovigilance Group, Medicines Control Agency, UK. leigh.henderson@mca.gsi.gov.uk

RESUMEN / SUMMARY:  - AIMS: The aim of this work is to identify the medicines which interact with the herbal remedy St John’s wort (SJW), and the mechanisms responsible. METHODS: A systematic review of all the available evidence, including worldwide published literature and spontaneous case reports provided by healthcare professionals and regulatory authorities within Europe has been undertaken. RESULTS: A number of clinically significant interactions have been identified with prescribed medicines including warfarin, phenprocoumon, cyclosporin, HIV protease inhibitors, theophylline, digoxin and oral contraceptives resulting in a decrease in concentration or effect of the medicines. These interactions are probably due to the induction of cytochrome P450 isoenzymes CYP3A4, CYP2C9, CYP1A2 and the transport protein P-glycoprotein by constituent(s) in SJW. The degree of induction is unpredictable due to factors such as the variable quality and quantity of constituent(s) in SJW preparations. In addition, possible pharmacodynamic interactions with selective serotonin re-uptake inhibitors and serotonin (5-HT(1d)) receptor-agonists such as triptans used to treat migraine were identified. These interactions are associated with an increased risk of adverse reactions. CONCLUSIONS: In Sweden and the UK the potential risks to patients were judged to be significant and therefore information about the interactions was provided to health care professionals and patients. The product information of the licensed medicines involved has been amended to reflect these newly identified interactions and SJW preparations have been voluntarily labelled with appropriate warnings.  N. Ref:: 44

 

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[125]

TÍTULO / TITLE:  - Treatment of gammaherpesvirus-related neoplastic disorders in the immunosuppressed host.

REVISTA / JOURNAL:  - Semin Hematol 2003 Apr;40(2):163-71.

      ●● Enlace al texto completo (gratuito o de pago) 1053/shem.2003.50016

AUTORES / AUTHORS:  - Little RF; Yarchoan R

INSTITUCIÓN / INSTITUTION:  - HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

RESUMEN / SUMMARY:  - Neoplastic disease is a frequent complication in patients with acquired immunodeficiency disease (AIDS) and other immunodeficiencies. Many such neoplasms are caused by either Epstein-Barr virus (EBV) or Kaposi’s sarcoma-associated herpes virus (KSHV). The treatment of such patients can be challenging. At the same time, the viral origin of these tumors offers targets to develop pathogenesis-based therapies. Standard therapies for these diseases involve such approaches as treating the underlying immunodeficiency, cytotoxic chemotherapy, and immunologic antitumor therapy. Novel therapy approaches include specific immune therapy and anti-angiogenesis approaches, now under development.  N. Ref:: 105

 

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[126]

TÍTULO / TITLE:  - Lichen planopilaris: report of 30 cases and review of the literature.

REVISTA / JOURNAL:  - Int J Dermatol 2003 May;42(5):342-5.

AUTORES / AUTHORS:  - Chieregato C; Zini A; Barba A; Magnanini M; Rosina P

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Verona University, Verona, Italy. carlochiergto@hotmail.com

RESUMEN / SUMMARY:  - BACKGROUND: Lichen planopilaris (LPP) affects primarily the scalp, resulting in scaling, atrophy, and alopecia with scarring. The purpose of our study was to obtain original data on LPP and to evaluate the efficacy of topical therapy in comparison with systemic therapies. METHODS: We examined 30 patients affected by LPP between 1996 and 2001, performing clinical, laboratory, histopathologic and direct immunofluorescence examinations. Twenty-one of the patients (70%) were women and nine (30%) were men. The average age at presentation was 51.5 years. The average duration of the disease was 13 months at the time of the diagnosis. All patients received topical steroids for a total of 12 weeks. RESULTS: Resolution of the inflammatory process and blocking of the cicatricial progression were observed in 66% of cases, a mild reduction of fibrosis and cicatrization in 20% of patients, and no response in 13%. CONCLUSIONS: We concluded that topical therapy may be a valid alternative to systemic therapies, especially in patients with lesions in the early phase.  N. Ref:: 6

 

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[127]

TÍTULO / TITLE:  - Risk factors for bronchiolitis obliterans: a systematic review of recent publications.

REVISTA / JOURNAL:  - J Heart Lung Transplant 2002 Feb;21(2):271-81.

AUTORES / AUTHORS:  - Sharples LD; McNeil K; Stewart S; Wallwork J

INSTITUCIÓN / INSTITUTION:  - Medical Research Council (MRC) Biostatistics Unit, University Forvie Site, Papworth Everard, Cambridge, United Kingdom. linda.sharples@mrc-bsu.cam.ac.uk

RESUMEN / SUMMARY:  - BACKGROUND: Obliterative bronchiolitis remains the major limitation to long-term survival after lung transplantation. A thorough understanding of the factors that confer high risk of developing obliterative bronchiolitis or its physiologic surrogate bronchiolitis obliterans syndrome is important to help define therapeutic strategies. METHODS: We performed a systematic review of studies published since the beginning of 1990. The review excluded non-human studies, publications before 1990, small (less than 25 patients) studies that were predominantly concerned with investigating the pathogenesis of obliterative bronchiolitis, studies solely concerned with diagnosis or treatment of obliterative bronchiolitis, and overlapping studies from the same center. Onset of bronchiolitis obliterans syndrome or obliterative bronchiolitis was the outcome of interest. RESULTS: Acute rejection plays an important role in obliterative bronchiolitis and bronchiolitis obliterans syndrome onset, and late rejection is a significant risk factor. Lymphocytic bronchitis/bronchiolitis is also a risk factor, with some evidence that late onset is associated with greater risk. The effects of cytomegalovirus, other infectious organisms, and human leukocyte antigen matching are less clear and require further confirmation. There is little evidence that recipient and donor characteristics play a major role. CONCLUSIONS: This systematic review supports the view that obliterative bronchiolitis arises from alloimmunologic injury marked by clinically apparent acute rejection episodes and that inflammatory conditions, including viral infections or ischemic injury, may also play a role. Implications for therapy are discussed.  N. Ref:: 28

 

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[128]

TÍTULO / TITLE:  - Long-term care of pediatric renal transplant patients: from bench to bedside.

REVISTA / JOURNAL:  - Curr Opin Pediatr 2002 Apr;14(2):205-10.

AUTORES / AUTHORS:  - Samsonov D; Briscoe DM

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, Department of Medicine, Children’s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.

RESUMEN / SUMMARY:  - In this review, we discuss current and future issues in the management of pediatric renal transplant recipients, including the optimization of long-term graft function and the minimization of complications caused by immunosuppression. Long-term management involves not only the monitoring of graft function but also the identification of patients at risk for the development of complications. The identification of patients with immunoreactive or immunoregulatory responses can be performed molecular monitoring of the immune response. Also, the use of frequent surveillance kidney biopsies, surrogate markers of chronic rejection, and glomerular filtration rate will be a part of future management. Identifying high-risk patients enables the physician to optimize immunosuppression to limit acute rejection. Short-and long-term management of pediatric transplant patients also includes adequate monitoring of growth and the monitoring for post-transplant lymphoproliferative disease. Ongoing clinical trials are underway that focus on these novel approaches in caring for pediatric transplant recipients.  N. Ref:: 41

 

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[129]

TÍTULO / TITLE:  - Minimizing calcineurin inhibitor drugs in renal transplantation.

REVISTA / JOURNAL:  - Transplant Proc 2003 May;35(3 Suppl):118S-121S.

AUTORES / AUTHORS:  - Flechner SM

INSTITUCIÓN / INSTITUTION:  - Section of Renal Transplantation, Transplant Center A110, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.

RESUMEN / SUMMARY:  - Calcineurin inhibitor drugs (CNI), primarily cyclosporine then tacrolimus, have been the centerpieces of maintenance immunosuppression for kidney transplantation since their introduction in the 1980s. While these drugs have been responsible for improved short-term outcomes and diminished rates of acute rejection, they are nephrotoxic and can cause permanent renal injury in many patients. Indeed, some have found that at 10 years after transplantation, the benefits of CNI drugs have been lost compared to the previous generation of maintenance immunosuppression. The use of these agents over many years contributes to the antigen-independent decline in renal function referred to as chronic allograft nephropathy. However, it remains unclear to what degree the use of CNI drugs contribute to ultimate graft loss. For these reasons immunosuppressive alternatives to CNI drugs have begun to emerge during the past few years. The recent introduction of the potent immunosuppressive agent sirolimus has afforded an opportunity to develop a regimen designed to maximize prophylaxis of early acute rejection, absent drug-induced nephrotoxicity. It was our feeling that the combination of antibody induction therapy combined with sirolimus substitution in a three-drug maintenance regimen, would provide the best posttransplant renal function and lowest rates of acute rejection. We have developed a CNI-free immunosuppressive regimen consisting of basiliximab induction, followed by sirolimus, MMF and steroids. Using this protocol we demonstrated comparable transplant outcomes with improved renal function in adult recipients of primary renal transplants. Limiting nephrotoxic immunosuppression should be considered an important goal; but requires sufficient long-term follow-up to support the benefits suggested from initial analysis of the data.  N. Ref:: 23

 

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[130]

TÍTULO / TITLE:  - Chronic graft-versus-host disease manifesting as polymyositis: an uncommon presentation.

REVISTA / JOURNAL:  - Bone Marrow Transplant 2002 Oct;30(8):543-6.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.bmt.1703711

AUTORES / AUTHORS:  - Couriel DR; Beguelin GZ; Giralt S; De Lima M; Hosing C; Kharfan-Dabaja MA; Anagnostopoulos A; Champlin R

INSTITUCIÓN / INSTITUTION:  - Department of Blood and Marrow Transplantation, University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA.

RESUMEN / SUMMARY:  - Graft-versus-host disease (GVHD) remains a major complication of allogeneic hematopoietic stem cell transplantation. Polymyositis can occur in association with chronic GVHD and mimics the idiopathic form of the disease. We report two cases of chronic GVHD-associated polymyositis and review the published literature. The two patients presented 13 and 19 months after allogeneic transplantation with characteristic features of muscular hypotrophy, proximal muscle weakness, pain, elevated creatine phosphokinase (CPK), aldolase and SGPT. Interestingly, both patients had HLA DR52 genes, which is frequently reported in association with idiopathic polymyositis. Electromyogram (EMG) and muscle biopsy confirmed the diagnosis. Treatment with cyclosporine or tacrolimus resulted in complete and sustained remission of polymyositis in both cases. A review of the literature shows cyclosporine and steroids are well-described treatment options for patients with myositis in post transplant, as well as idiopathic cases. The duration of immunosuppressive treatment has varied in different reports, and there is a risk of recurrence when immunosuppression is tapered.  N. Ref:: 32

 

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[131]

TÍTULO / TITLE:  - FTY720: targeting G-protein-coupled receptors for sphingosine 1-phosphate in transplantation and autoimmunity.

REVISTA / JOURNAL:  - Curr Opin Immunol 2002 Oct;14(5):569-75.

AUTORES / AUTHORS:  - Brinkmann V; Lynch KR

INSTITUCIÓN / INSTITUTION:  - Novartis Pharma AG Transplantation Research WSJ-386.101, CH-4002 Basel, Switzerland. volker.brinkmann@pharma.novartis.com

RESUMEN / SUMMARY:  - The novel immunomodulator FTY720 is remarkably effective in models of transplantation and autoimmunity. Recent data show that phosphorylated FTY720 is an agonist at four sphingosine 1-phosphate receptors. Stimulation of sphingosine 1-phosphate receptors leads to sequestration of lymphocytes in secondary lymphatic tissues and thus away from inflammatory lesions and graft sites.  N. Ref:: 44

 

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[132]

TÍTULO / TITLE:  - Exploring treatment options in renal transplantation: the problems of chronic allograft dysfunction and drug-related nephrotoxicity.

REVISTA / JOURNAL:  - Transplantation 2001 Jun 15;71(11 Suppl):SS42-51.

AUTORES / AUTHORS:  - Campistol JM; Grinyo JM

INSTITUCIÓN / INSTITUTION:  - University of Barcelona, España.

RESUMEN / SUMMARY:  - The immunosuppressive benefits of cyclosporine and tacrolimus in short-term and medium-term renal allograft survival are well documented. It is becoming increasingly clear that the basis of this immunosuppression, the inhibition of calcineurin, may be linked with nephrotoxicity, hypertension, hyperlipidemia, and new-onset diabetes mellitus, side effects that may lead to CRAD, death due to CVD, and late renal allograft loss. This clinical picture presents a clear need for new strategies that produce adequate immunosuppression to prevent acute rejection while simultaneously reducing the side effects associated with CNI-related therapies. Sirolimus combined with cyclosporine and tacrolimus has demonstrated an ability to reduce incidences of early acute rejection and, used as base therapy, has provided protection against acute rejection equivalent to that of cyclosporine, without the consequent nephrotoxicity associated with CNIs. In preliminary results from an ongoing clinical trial, sirolimus has been used to eliminate cyclosporine during maintenance immunosuppression, with subsequent improvements in measures of blood pressure and renal function. In addition, the antiproliferative properties of sirolimus and its ability to prevent graft vascular disease in animal studies make sirolimus a promising agent to decrease incidences of CRAD and improve long-term renal allograft survival. These findings point to a clear need to further explore both the efficacy of sirolimus immunotherapy and its long-term effects.  N. Ref:: 126

 

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[133]

TÍTULO / TITLE:  - Histopathological study of intrahepatic islets transplanted in the nonhuman primate model using edmonton protocol immunosuppression.

REVISTA / JOURNAL:  - J Clin Endocrinol Metab. Acceso gratuito al texto completo a partir de 1 año de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://jcem.endojournals.org/ 

      ●● Cita: J. of Clin Endocrinol & Metab: <> 2002 Dec;87(12):5424-9.

AUTORES / AUTHORS:  - Hirshberg B; Mog S; Patterson N; Leconte J; Harlan DM

INSTITUCIÓN / INSTITUTION:  - Transplantation and Autoimmunity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

RESUMEN / SUMMARY:  - While islet cell transplantation is a promising way to restore insulin independence to patients with type I diabetes mellitus, a detailed histological analysis of the transplanted, intraportal islets has not yet been reported. Rhesus macaques underwent total pancreatectomy, then had allogeneic isolated islets infused into their portal vein, followed by daclizumab, tacrolimus, and sirolimus to prevent islet rejection. Islets were evenly distributed among the liver lobes. Liver sections from a primate given allogeneic islets 5 d earlier did not display any islet capillary formation, whereas intrahepatic islets transplanted 30 and 90 d before euthanasia showed an abundant capillary supply. Localized hepatocellular glycogenosis was observed surrounding the islets in a primate with functioning islets 7 months post transplant. Liver sections from a primate that rejected islets transplanted 2 months prior displayed only islet remnants with prominent local lymphohistiocytic inflammation and an occasional capillary. We conclude that islets develop an abundant vascular supply within 30 d following transplant and because capillaries persist even following rejection, that the vascular cells are likely from the recipient. While transplanted islets were not vascularized early post transplant, the primates remained insulin independent. The long-term consequence of islets in the liver, marked by the glycogenosis, remains unknown and warrants further study.

 

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[134]

TÍTULO / TITLE:  - Resolution of oral non-Hodgkin’s lymphoma by reduction of immunosuppressive therapy in a renal allograft recipient: a case report and review of the literature.

REVISTA / JOURNAL:  - Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002 Dec;94(6):697-701.

      ●● Enlace al texto completo (gratuito o de pago) 1067/moe.2002.126889

AUTORES / AUTHORS:  - Keogh PV; Fisher V; Flint SR

INSTITUCIÓN / INSTITUTION:  - Department of Oral Surgery, Oral Medicine and Oral Pathology, Dublin Dental School and Hospital, Trinity College, Ireland. pakeogh@dental.tcd.ie

RESUMEN / SUMMARY:  - A case of oral non-Hodgkin’s lymphoma arising in a patient with insulin-dependent diabetes who had undergone renal allograft transplantation is described. The resolution of the disease was achieved by a reduction in her immunosuppressive therapy. The differential diagnosis is discussed, and the management of posttransplantation lymphoproliferative disorders is reviewed.  N. Ref:: 40

 

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[135]

TÍTULO / TITLE:  - The mucosa of the small intestine: how clinically relevant as an organ of drug metabolism?

REVISTA / JOURNAL:  - Clin Pharmacokinet 2002;41(4):235-53.

AUTORES / AUTHORS:  - Doherty MM; Charman WN

INSTITUCIÓN / INSTITUTION:  - Department of Pharmaceutics, Victorian College of Pharmacy, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia. margaret.doherty@vcp.monash.edu.au

RESUMEN / SUMMARY:  - The intestinal mucosa is capable of metabolising drugs via phase I and II reactions. Increasingly, as a result of in vitro and in vivo (animal and human) data, the intestinal mucosa is being implicated as a major metabolic organ for some drugs. This has been supported by clinical studies of orally administered drugs (well-known examples include cyclosporin, midazolam, nifedipine and tacrolimus) where intestinal drug metabolism has significantly reduced oral bioavailability. This review discusses the intestinal properties and processes that contribute to drug metabolism. An understanding of the interplay between the processes controlling absorption, metabolism and P-glycoprotein-mediated efflux from the intestinal mucosa into the intestinal lumen facilitates determination of the extent of the intestinal contribution to first-pass metabolism. The clinical relevance of intestinal metabolism, however, depends on the relative importance of the metabolic pathway involved, the therapeutic index of the drug and the inherent inter- and intra-individual variability. This variability can stem from genetic (metabolising enzyme polymorphisms) and/or non-genetic (including concomitant drug and food intake, route of administration) sources. An overwhelming proportion of clinically relevant drug interactions where the intestine has been implicated as a major contributor to first-pass metabolism involve drugs that undergo cytochrome P450 (CYP) 3A4-mediated biotransformation and are substrates for the efflux transporter P-glycoprotein. Much work is yet to be done in characterising the clinical impact of other enzyme systems on drug therapy. In order to achieve this, the first-pass contributions of the intestine and liver must be successfully decoupled.  N. Ref:: 130

 

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[136]

TÍTULO / TITLE:  - Transplantation: toxicokinetics and mechanisms of toxicity of cyclosporine and macrolides.

REVISTA / JOURNAL:  - Curr Opin Investig Drugs 2003 Nov;4(11):1287-96.

AUTORES / AUTHORS:  - Serkova N; Christians U

INSTITUCIÓN / INSTITUTION:  - Department of Anesthesiology, Clinical Research & Development, University of Colorado Health Sciences Center, 4200 East Ninth Ave, Room UH-2122, Campus Box B113, Denver, CO 80262, USA.

RESUMEN / SUMMARY:  - For over two decades, calcineurin inhibitors (CIs) have been the mainstay of immunosuppressive therapy following solid-organ transplantation. However, CI nephrotoxicity is one of the main contributors to chronic kidney allograft dysfunction. A novel class of immunosuppressants that inhibit the kinase mammalian target of rapamycin (mTOR), although not nephrotoxic themselves, enhance CI nephrotoxicity. The biochemical basis of CI toxicity and their toxicodynamic interaction with mTOR inhibitors is still poorly understood. Studies using a magnetic resonance spectroscopy-based metabonomic approach indicate that CI toxicity is caused by drug-induced mitochondrial dysfunction and that mTOR inhibitors enhance the negative effects of CIs on cell energy metabolism.  N. Ref:: 77

 

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[137]

TÍTULO / TITLE:  - Risk factors for and management of post-transplantation cardiovascular disease.

REVISTA / JOURNAL:  - BioDrugs 2001;15(4):261-78.

AUTORES / AUTHORS:  - Fellstrom B

INSTITUCIÓN / INSTITUTION:  - Department of Medical Sciences, University Hospital, SE-751 85 Uppsala, Sweden. bengt.fellstrom@medsci.uu.se

RESUMEN / SUMMARY:  - The mortality rates due to cardiovascular disease (CVD) in transplant recipients are greater than in the general population. CVD is a major cause of both graft loss and patient death in renal transplant recipients, and improving cardiovascular health in transplant recipients will presumably help to extend both patient and graft survival. Further studies are needed to better evaluate the effectiveness of risk modification on subsequent CVD morbidity and mortality. There is no reason to consider risk factors for CVD such as hyperlipidaemia, hypertension and diabetes mellitus in transplant recipients differently from in the general population. In addition, there are specific transplantation risk factors such as acute rejection episodes and the use of immunosuppressive drugs. It is obvious that several of the immunosuppressive agents used today have disadvantageous influences on risk factors for CVD such as hyperlipidaemia, hypertension and post-transplantation diabetes mellitus (PTDM), but the relative importance of immunosuppressant-induced increases in these risk factors is basically unknown. This may be a strong argument for the selective use and individual tailoring of immunosuppressive agents based upon the risk factor profile of the patient, without jeopardising the function of the graft. Hyperlipidaemia is common after transplantation, and immunosuppression with corticosteroids, cyclosporin, or sirolimus (rapamycin) causes different types of post-transplantation hyperlipidaemia. However, to date, no studies have demonstrated that lipid lowering strategies significantly reduce CVD morbidity or mortality and improve allograft survival in transplant recipients. Several studies using preventive or interventional approaches are ongoing and will be reported in the near future. Post-transplantation hypertension appears to be a major risk factor determining graft and patient survival, and immunosuppressive agents have different effects on hypertension. Controlled studies support the opinion that post-transplantation hypertension must be treated as strictly as in a population with essential hypertension, diabetes mellitus, or chronic renal failure. As increasing numbers of immunosuppressive agents become available for use, we may be in a better position to tailor immunosuppressive therapy to the individual patient, avoiding the use of diabetogenic drugs, drug combinations, or inappropriate doses in patients susceptible to PTDM. Multiple acute rejection episodes have also been demonstrated to be a risk factor for CVD - a strong argument for the use of immunosuppressive drugs to reduce acute rejection. Until we have a better understanding from ongoing landmark studies on the management of CVD, presently available therapy to reduce risk factors needs to be used together with individual tailoring of immunosuppressive therapy with the aim of reducing CVD in these patients.  N. Ref:: 138

 

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[138]

TÍTULO / TITLE:  - The cytology of HIV-induced immunosuppression. Changing pattern of disease in the era of highly active antiretroviral therapy.

REVISTA / JOURNAL:  - Cytopathology 2001 Oct;12(5):281-96.

AUTORES / AUTHORS:  - Kocjan G; Miller R

INSTITUCIÓN / INSTITUTION:  - Department of Histopathology, Royal Free and University College Medical School, University College London, UK.  N. Ref:: 89

 

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[139]

TÍTULO / TITLE:  - The effect of immunosuppressive protocols on spontaneous CNS remyelination following toxin-induced demyelination.

REVISTA / JOURNAL:  - J Neuroimmunol 2001 Oct 1;119(2):261-8.

AUTORES / AUTHORS:  - Smith PM; Franklin RJ

INSTITUCIÓN / INSTITUTION:  - Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK.

RESUMEN / SUMMARY:  - Glial cell transplantation is a potential therapy for human demyelinating disease, though obtaining large numbers of human myelinating cells for transplantation remains a major stumbling block. Autologous transplantation is currently not possible, since the adult human CNS is not a good source of oligodendrocyte precursors, and long-term immunosuppression of engrafted allogeneic or xenogeneic cells is therefore likely to be necessary. Immunosuppressive drugs may need to be used in situations where more recent, active areas of demyelination are undergoing endogenous remyelination. It is therefore pertinent to establish the extent to which immunosuppressive protocols will suppress spontaneous remyelination. In order to investigate this issue, we created demyelinating lesions in the spinal cord of adult rats and compared the extent of remyelination in animals receiving different immunosuppressive treatments. In animals given only cyclosporin A, there was no difference in the extent of either Schwann cell or oligodendrocyte remyelination of ethidium bromide-induced demyelinating lesions. However, in animals given cyclophosphamide, either alone or in combination with cyclosporin, there was a significant reduction in the extent of oligodendrocyte-mediated remyelination. These results demonstrate that cyclophosphamide is deleterious to oligodendrocyte remyelination and for this reason should be used with caution in patients with demyelinating disease.

 

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[140]

TÍTULO / TITLE:  - Non-steroidal topical immunomodulators provide skin-selective, self-limiting treatment in atopic dermatitis.

REVISTA / JOURNAL:  - Eur J Dermatol 2003 Sep-Oct;13(5):455-61.

AUTORES / AUTHORS:  - Bos JD

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology A0-235, Academic Medical Centre, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands. j.d.bos@amc.uva.nl

RESUMEN / SUMMARY:  - Topical corticosteroids are the mainstay of treatment for atopic dermatitis; however, their clinical utility is limited by potential side effects. Recently, the steroid-free topical immunomodulators tacrolimus ointment and pimecrolimus cream have become available. These agents provide effective treatment without causing skin atrophy or other steroidal side effects, and their physiochemical properties, such as relatively large molecular size and high lipophilicity, limit diffusion through skin and into the bloodstream, providing skin-selective treatment. Clinical trials with more than 1,700 paediatric and adult patients have demonstrated that treatment with either agent is associated with minimal systemic absorption of tacrolimus or pimecrolimus. Additionally, studies have shown that percutaneous absorption of tacrolimus decreases as treatment continues and clinical improvement occurs. This self-limiting facet of the treatment, together with the skin-selectivity of topical immunomodulators, is reflected in the good safety and tolerability profiles of these agents, which promise to significantly improve the long-term management of atopic dermatitis.  N. Ref:: 56

 

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[141]

TÍTULO / TITLE:  - Bilateral keratoconjunctivitis associated with lichen planus.

REVISTA / JOURNAL:  - Cornea 2004 Jan;23(1):100-5.

AUTORES / AUTHORS:  - Rhee MK; Mootha VV

INSTITUCIÓN / INSTITUTION:  - Eye and Ear Institute of Pittsburgh, University of Pittsburgh, Pittsburgh, PA, USA.

RESUMEN / SUMMARY:  - PURPOSE: To describe a case of bilateral keratoconjunctivitis in a patient with lichen planus. METHODS: Case report and review of the English literature. RESULTS: To our knowledge, this is the fourth reported case of keratoconjunctivitis associated with lichen planus. A 33-year-old Navajo man with lichen planus had recurrent and progressive keratoconjunctivitis that failed to improve on multiple topical medications. Tapered oral prednisone, 2% topical cyclosporin, and amniotic membrane transplantation pacified the acute exacerbation. CONCLUSIONS: Our patient with lichen planus developed an ocular surface disease with cicatricial conjunctivitis, keratouveitis, keratoconjunctivitis sicca, punctate epithelial erosions, and persistent epithelial defects leading to noninfectious or infectious corneal ulceration. Amniotic membrane transplantation may play an adjunctive role in refractory cases of lichen planus-related keratoconjunctivitis. Topical cyclosporin may stabilize the ocular surface when combined with systemic immunosuppression in severe cases.  N. Ref:: 20

 

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[142]

TÍTULO / TITLE:  - Therapeutic drug monitoring of immunosuppressant drugs in clinical practice.

REVISTA / JOURNAL:  - Clin Ther 2002 Mar;24(3):330-50; discussion 329.

AUTORES / AUTHORS:  - Kahan BD; Keown P; Levy GA; Johnston A

INSTITUCIÓN / INSTITUTION:  - Division of Immunology and Organ Transplantation, University of Texas Health Science Center at Houston Medical School, 77030, USA. Barry.D.Kahan@uth.tmc.edu

RESUMEN / SUMMARY:  - BACKGROUND: Therapeutic drug monitoring (TDM) is essential to maintain the efficacy of many immunosuppressant drugs while minimizing their toxicity. TDM has become more refined with the development of new monitoring techniques and more specific assays. OBJECTIVE: This article summarizes current data on TDM of the following immunosuppressant drugs used in organ transplantation: cyclosporine, tacrolimus, sirolimus, everolimus, and mycophenolate mofetil. METHODS: Published data were identified by a MEDLINE search of the English-language literature through March 2001 using the terms therapeutic drug monitoring, cyclosporine, tacrolimus, sirolimus, everolimus, and mycophenolate mofetil. Relevant conference abstracts were also included. RESULTS: TDM of cyclosporine has been well studied, and recent findings indicate that monitoring of drug levels 2 hours after dosing is a more sensitive predictor of outcome than trough (C0) monitoring. C0 levels are being used more widely in TDM of tacrolimus; however, the relationship between C0 and area under the curve has varied widely in clinical trials, with correlations ranging from 0.11 to 0.92. The use of TDM of sirolimus, everolimus, and mycophenolate mofetil is evolving rapidly. CONCLUSIONS: TDM of immunosuppressant drugs that have a narrow therapeutic index is an increasingly useful tool for minimizing drug toxicity while maximizing prevention of graft loss and organ rejection.  N. Ref:: 85

 

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[143]

TÍTULO / TITLE:  - Immunosuppressive and cytotoxic drugs in the treatment of rheumatic skin disorders.

REVISTA / JOURNAL:  - Semin Cutan Med Surg 2001 Mar;20(1):58-68.

AUTORES / AUTHORS:  - Callen JP

INSTITUCIÓN / INSTITUTION:  - Division of Dermatology, University of Louisville School of Medicine, KY 40202, USA. jefca@aol.com

RESUMEN / SUMMARY:  - Cytotoxic and immunosuppressive drugs are regularly used to treat proliferative, immunologically mediated inflammatory disorders and some neoplastic diseases of the skin. Methotrexate, azathioprine, mycophenolate mofetil, cyclosporin cyclophosphamide, chlorambucil, and other related drugs have potential benefits in the treatment of severe and/or recalcitrant rheumatic skin diseases. The therapeutic window for these agents is narrow. The major uses of these drugs are for life-threatening cutaneous disorders or as steroid-sparing agents.  N. Ref:: 57

 

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[144]

TÍTULO / TITLE:  - Drug-eluting stents in peripheral vascular disease: eliminating restenosis.

REVISTA / JOURNAL:  - Mt Sinai J Med. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.mssm.edu/msjournal/ 

      ●● Cita: Mount Sinai J. of Medicine, New York: <> 2003 Nov;70(6):417-9.

AUTORES / AUTHORS:  - Ellozy SH; Carroccio A

INSTITUCIÓN / INSTITUTION:  - Department of Surgery, Mount Sinai School of Medicine, One East 100th Street, New York, NY 10029, USA. sharif.ellozy@msnyuhealth.org

RESUMEN / SUMMARY:  - Transcatheter endovascular therapy for peripheral atherosclerotic disease has become more popular. In general, good results have been reported in focal aortoiliac disease. However, the long-term patency of angioplasty in longer, more distal lesions has been less satisfactory. Stenting has not been shown to improve long-term patency compared to angioplasty alone. Drug-eluting stents have shown promise in preventing coronary restenosis, and preliminary results in peripheral arterial disease are encouraging. This review article will discuss the current status of endovascular therapy of aortoiliac and femoropopliteal atherosclerotic disease, the theoretic and experimental basis for the use of drug-eluting stents, and the preliminary results in human studies.  N. Ref:: 48

 

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[145]

TÍTULO / TITLE:  - Cyclosporine in the treatment of idiopathic focal segmental glomerulosclerosis.

REVISTA / JOURNAL:  - Semin Nephrol 2003 Mar;23(2):234-41.

      ●● Enlace al texto completo (gratuito o de pago) 1053/snep.2003.50022

AUTORES / AUTHORS:  - Cattran DC

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, Toronto General Hospital, University Health Network, Toronto, Ontario, Canada. daniel.cattran@uhn.on.ca

RESUMEN / SUMMARY:  - Cyclosporine a known powerful immunosuppressive medication and has been used in the treatment of focal segmental glomerulosclerosis (FSGS) for over a decade. Its precise mechanism of action in this disorder is still debated and is likely at more than one level related to the pathophysiology of the disease. Multiple studies have been performed but the numbers of randomized trials of this drug in this disease are very limited. However, both the best studies in children and adults indicate in the steroid-resistant patients that 50% to 70% will have a response in terms of a significant reduction in proteinuria. Provided the total dose is kept to 5 mg/kg or less and duration to less than 12 months, the drug is safe but careful monitoring is required to maintain the blood pressure at ideal levels and to avoid nephrotoxicity. Relapses are common, but rather than considering this a failure of therapy the drug should be reintroduced because in most cases it will reestablish control of the proteinuria. Although in the past cyclosporine has been considered a second-line agent in FSGS, emerging data would suggest in the high-risk patients related to corticosteroid toxicity it should be considered primary therapy.  N. Ref:: 37

 

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[146]

TÍTULO / TITLE:  - The potential of chitosan in ocular drug delivery.

REVISTA / JOURNAL:  - J Pharm Pharmacol 2003 Nov;55(11):1451-63.

      ●● Enlace al texto completo (gratuito o de pago) 1211/0022357022476

AUTORES / AUTHORS:  - Alonso MJ; Sanchez A

INSTITUCIÓN / INSTITUTION:  - Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, España. ffmjalon@usc.es

RESUMEN / SUMMARY:  - This paper presents an overview of the potential of chitosan-based systems for improving the retention and biodistribution of drugs applied topically onto the eye. Besides its low toxicity and good ocular tolerance, chitosan exhibits favourable biological behaviour, such as bioadhesion- and permeability-enhancing properties, and also interesting physico-chemical characteristics, which make it a unique material for the design of ocular drug delivery vehicles. The review summarizes the techniques for the production of chitosan gels, chitosan-coated colloidal systems and chitosan nanoparticles, and describes their mechanism of action upon contact with the ocular mucosa. The results reported until now have provided evidence of the potential of chitosan gels for enhancing and prolonging the retention of drugs on the eye surface. On the other hand, chitosan-based colloidal systems were found to work as transmucosal drug carriers, either facilitating the transport of drugs to the inner eye (chitosan-coated colloidal systems containing indometacin) or their accumulation into the corneal/conjunctival epithelia (chitosan nanoparticles containing ciclosporin). Finally, the tolerance, toxicity and biodegradation of the carriers under evaluation were reviewed.  N. Ref:: 75

 

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[147]

TÍTULO / TITLE:  - Rejection rate in living donor kidney transplantation with and without basiliximab in tacrolimus/mycophenolate mofetil-based protocol.

REVISTA / JOURNAL:  - Transplant Proc 2003 Mar;35(2):653-4.

AUTORES / AUTHORS:  - Rahamimov R; Yussim A; After T; Lustig S; Bar-Nathan N; Shaharabani E; Shapira Z; Shabthai E; Mor E

INSTITUCIÓN / INSTITUTION:  - Department of Transplantation, Rabin Medical Center, Beilinson Campus, Petah-Tiqwa, Israel. rutir@clalit.org.il

 

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[148]

TÍTULO / TITLE:  - B19 virus infection in renal transplant recipients.

REVISTA / JOURNAL:  - J Clin Virol. Acceso gratuito al texto completo.

      ●● Enlace a la Editora de la Revista http://www.elsevier.com/gej-ng/29/46/32/show/Products/VIRUSINT/index.htt 

      ●● Cita: J Clinical Virology: <> 2003 Apr;26(3):361-8.

AUTORES / AUTHORS:  - Cavallo R; Merlino C; Re D; Bollero C; Bergallo M; Lembo D; Musso T; Leonardi G; Segoloni GP; Ponzi AN

INSTITUCIÓN / INSTITUTION:  - Virology Unit, Department of Public Health and Microbiology, University of Turin, Via Santena 9, 10126, Turin, Italy. rossana.cavallo@unito.it

RESUMEN / SUMMARY:  - BACKGROUND: B19 virus infection with persistent anaemia has been reported in organ transplant recipients. Detection of B19 virus DNA in serum is the best direct marker of active infection. OBJECTIVE: The present study evaluated the incidence and clinical role of active B19 virus infection in renal transplant recipients presenting with anaemia. STUDY DESIGN: Forty-eight such recipients were investigated by nested PCR on serum samples. The controls were 21 recipients without anaemia. Active HCMV infection was also investigated as a marker of high immunosuppression. RESULTS AND CONCLUSIONS: In 11/48 (23%) patients B19 virus DNA was demonstrated in serum versus only 1/21 (5%) of the controls. Ten of these 11 patients had already been seropositive at transplantation and active infection occurred in eight of them during the first 3 months after transplantation. The remaining patient experienced a primary infection 9 months after transplantation. Eight (73%) of these 11 patients displayed a concomitant HCMV infection and four (36%) showed increasing serum creatinine levels but none developed glomerulopathy; 3/11 (27%) recovered spontaneously from anaemia whereas 8/11 (73%) needed therapy. In conclusion, the relatively high occurrence (23%) of B19 virus infection in patients presenting with anaemia, suggests that it should be considered in the differential diagnosis of persistent anaemia in renal transplant recipients. Presence of the viral DNA should be assessed early from transplantation and the viral load should be monitored to follow persistent infection and better understand the relation between active infection and occurrence of anaemia, and to assess the efficacy of IVIG therapy and/or immunosuppression reduction in clearing the virus.  N. Ref:: 56

 

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[149]

- Castellano -

TÍTULO / TITLE:Nuevos tratamientos inmunodepresores de induccion en el trasplante renal. New induction immunosuppression treatments in kidney transplantation.

REVISTA / JOURNAL:  - Med Clin (Barc). Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://db.doyma.es/ 

      ●● Cita: Medicina Clínica: <> 2001 Jun 30;117(4):147-57.

AUTORES / AUTHORS:  - Pascual J; Ortuno J

INSTITUCIÓN / INSTITUTION:  - Servicio de Nefrologia. Universidad de Alcala. Hospital Ramon y Cajal. Madrid. jpascual@hrc.insalud.es  N. Ref:: 94

 

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[150]

TÍTULO / TITLE:  - Therapeutic monitoring of mycophenolate mofetil in organ transplant recipients: is it necessary?

REVISTA / JOURNAL:  - Clin Pharmacokinet 2002;41(5):319-27.

AUTORES / AUTHORS:  - Mourad M; Wallemacq P; Konig J; de Frahan EH; Eddour DC; De Meyer M; Malaise J; Squifflet JP

INSTITUCIÓN / INSTITUTION:  - Department of Kidney and Pancreas Transplantation, University Hospital Saint Luc, Universite Catholique de Louvain, Brussels, Belgium. Michel.Mourad@chir.ucl.ac.be

RESUMEN / SUMMARY:  - Adequate immunosuppression minimising the risk of organ rejection with acceptable tolerability of the used drugs is a crucial step in organ transplantation. The primary goal is to maintain a consistent time-dependent target concentration by tailoring individual dosage leading to the best efficacy and tolerability combination. The use of therapeutic drug monitoring (TDM) to optimise immunosuppressive therapy is routinely employed for maintenance drugs such as cyclosporin and tacrolimus. The question whether therapeutic monitoring of mycophenolic acid (MPA) in organ transplant recipients treated with mycophenolate mofetil is necessary is not definitely answered. The correlation of mycophenolic acid pharmacokinetic parameters with efficacy and toxicity makes the therapeutic monitoring of this drug promising. However, further studies are mandatory to draw the best guidelines in order to achieve higher levels of evidence that MPA-TDM may improve patient outcome.  N. Ref:: 63

 

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[151]

TÍTULO / TITLE:  - Osteoporosis after solid organ and bone marrow transplantation.

REVISTA / JOURNAL:  - Osteoporos Int 2003 Aug;14(8):617-30. Epub 2003 Aug 8.

      ●● Enlace al texto completo (gratuito o de pago) 1007/s00198-003-1426-z

AUTORES / AUTHORS:  - Cohen A; Shane E

INSTITUCIÓN / INSTITUTION:  - College of Physicians & Surgeons of Columbia University, 630 West 168th Street, New York, NY 10032, USA.

RESUMEN / SUMMARY:  - Organ transplantation has become increasingly common as a therapy for end-stage renal, liver, cardiac and pulmonary disease. The population of patients who have survived organ transplantation has grown dramatically over the last 2 decades. Although organ transplant recipients now benefit from greatly improved survival, long-term complications of organ transplantation, such as osteoporosis, adversely affect quality of life and must be addressed. In the early post-transplantation period, the effects of high dose glucocorticoids, combined with other immunosuppressive drugs such as cycosporine A and tacrolimus, cause rapid bone loss particularly at the spine and proximal femur. In this setting, fracture incidence rates as high as 25-65% have been reported. Treatment and prevention strategies must target this early post-transplant period, as well as the patient awaiting transplantation and the long-term transplant recipient. This review will discuss the clinical features of transplantation osteoporosis, the pathophysiology of post-transplantation bone loss and prevention and therapy of this unique bone disease.  N. Ref:: 182

 

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[152]

TÍTULO / TITLE:  - The Rapamune era of immunosuppression 2003: the journey from the laboratory to clinical transplantation.

REVISTA / JOURNAL:  - Transplant Proc 2003 May;35(3 Suppl):18S-24S.

AUTORES / AUTHORS:  - Camardo J

INSTITUCIÓN / INSTITUTION:  - Wyeth Research, Collegeville, Pennsylvania 19426, USA.

RESUMEN / SUMMARY:  - The story of Rapamune (sirolimus, rapamycin) began with the isolation of an antibiotic from a soil sample sent to Ayerst Laboratories in Montreal. More than 25 years later, sirolimus was approved for use by transplant physicians in the United States. Development programs for new drugs for transplantation face significant challenges. Four key challenges were critical to the development of sirolimus as a drug for transplantation: First, sirolimus was not intended to be an antirejection agent. Second, sirolimus was not easy to make or purify into a palatable substance for human use and the development of a pharmaceutical form was an important and critical hurdle. Third, sirolimus showed potent antirejection activity when tested in de novo allograft recipients, but the development program required careful attention to its optimal use in multidrug transplant regimens. Fourth, the clinical program approved in the United States was rejected in Europe, and it was only with additional studies and a unique appeal process that sirolimus became available in Europe. Currently, sirolimus (Rapamune) is available throughout most of the world except in Japan, having achieved regulatory approvals in North America, Europe, the Middle East, Latin America, and Asia. Although sirolimus failed in its original role as an antifungal agent, it ultimately succeeded as an antirejection drug. Today, sirolimus holds additional promise both as a drug useful for the prevention of restenosis after coronary angioplasty and as an antitumor agent.  N. Ref:: 39

 

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[153]

TÍTULO / TITLE:  - Cicatricial pemphigoid in the upper aerodigestive tract: diagnosis and management in severe laryngeal stenosis.

REVISTA / JOURNAL:  - Ann Otol Rhinol Laryngol 2003 Mar;112(3):271-5.

AUTORES / AUTHORS:  - Boedeker CC; Termeer CC; Staats R; Ridder GJ

INSTITUCIÓN / INSTITUTION:  - Department of Otorhinolaryngology-Head and Neck Surgery, Albert-Ludwigs-University, Freiburg, Germany.

RESUMEN / SUMMARY:  - Pemphigoid is a group of rare, acquired, autoimmune subepithelial blistering diseases. The condition has been subclassified into bullous pemphigoid and cicatricial pemphigoid (CP). Diagnosis is based on clinical presentation, evidence of subepithelialvesicles or bullae on routine histologic analysis, and direct and indirect immunofluorescence studies. Cicatricial pemphigoid is characterized by linear deposition of immunoreactants, principally IgG and complement factor 3, along epithelial basement membranes. Cicatricial pemphigoid usually leads to mucosal scarring. We present a case of severe CP that led to laryngeal and subglottic stenosis and involvement of both eyes and the oral, nasal, and nasopharyngeal mucosae. Treatment with dapsone, corticosteroids, azathioprine sodium, cyclosporine A, cyclophosphamide, methotrexate sodium, and mycophenolate mofetil between 1997 and 2001 only resulted in temporary disease control. The patient has been treated with leflunomide for the past 8 months, and there have been no relapses. Treatment of CP with leflunomide has not been described in the literature until now.  N. Ref:: 25

 

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[154]

TÍTULO / TITLE:  - Molecular diagnosis of an Enterocytozoon bieneusi human genotype C infection in a moderately immunosuppressed human immunodeficiency virus seronegative liver-transplant recipient with severe chronic diarrhea.

REVISTA / JOURNAL:  - J Clin Microbiol. Acceso gratuito al texto completo a partir de los 6 meses de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://jcm.asm.org/ 

      ●● Cita: J. Clinical Microbiology: <> 2001 Jun;39(6):2371-2.

AUTORES / AUTHORS:  - Sing A; Tybus K; Heesemann J; Mathis A  N. Ref:: 5

 

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[155]

TÍTULO / TITLE:  - Rapamycin: friend, foe, or misunderstood?

REVISTA / JOURNAL:  - Liver Transpl 2003 May;9(5):469-72.

      ●● Enlace al texto completo (gratuito o de pago) 1053/jlts.2003.50101

AUTORES / AUTHORS:  - Fung J; Marcos A  N. Ref:: 19

 

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[156]

TÍTULO / TITLE:  - Eradication of parvovirus B19 infection after renal transplantation requires reduction of immunosuppression and high-dose immunoglobulin therapy.

REVISTA / JOURNAL:  - Nephrol Dial Transplant. Acceso gratuito al texto completo a partir de los 2 años de la fecha de publicación.

      ●● Enlace a la Editora de la Revista http://ndt.oupjournals.org/ 

      ●● Cita: Nephrology Dialysis Transplantation: <> 2002 Oct;17(10):1840-2.

AUTORES / AUTHORS:  - Liefeldt L; Buhl M; Schweickert B; Engelmann E; Sezer O; Laschinski P; Preuschof L; Neumayer HH

INSTITUCIÓN / INSTITUTION:  - Department of Nephrology, Charite, Humboldt-University Berlin, Germany. lutz.liefeldt@charite.de  N. Ref:: 17

 

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[157]

TÍTULO / TITLE:  - Recent advances in immunosuppressive therapy for renal transplantation.

REVISTA / JOURNAL:  - Semin Dial 2001 May-Jun;14(3):218-22.

AUTORES / AUTHORS:  - Peddi VR; First MR

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology and Hypertension, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0585, USA. ram.peddi@uc.edu

RESUMEN / SUMMARY:  - Recent advances in immunosuppression have focused on more effective, safer, and targeted therapies that have resulted in improved short- and intermediate-term renal allograft survival. During the past decade there has been a marked decrease in acute rejection rates following renal transplantation because of the use of newer immunosuppressive agents. Recent data indicate that the average yearly reduction in the relative hazard of graft failure beyond 1 year was 4.2% for all recipients (0.4% for those recipients who had an acute rejection episode and 6.3% for those who did not have an acute rejection). Despite these improvements the currently available immunosuppressive agents are associated with significant cardiovascular risk factors, an increased risk of infection, and the development of malignancies in the long term. Predictive parameters of donor-specific hyporesponsiveness are needed so as to allow identification of patients in whom immunosuppressive therapy can be safely reduced. Immunosuppressive agents that have recently been approved for use in the United States and those that are in clinical and preclinical studies are discussed.  N. Ref:: 27

 

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[158]

TÍTULO / TITLE:  - Impact of immunosuppressive therapy on recurrence of hepatitis C.

REVISTA / JOURNAL:  - Liver Transpl 2002 Oct;8(10 Suppl 1):S19-27.

      ●● Enlace al texto completo (gratuito o de pago) 1053/jlts.2002.35852

AUTORES / AUTHORS:  - Everson GT

INSTITUCIÓN / INSTITUTION:  - Hepatology, University of Colorado School of Medicine, Denver, CO 80262, USA. greg.everson@ucshc.edu

RESUMEN / SUMMARY:  - 1. Approximately 10% to 25% of hepatitis C virus-infected recipients of liver allografts will develop cirrhosis within 5 years of transplantation; this acceleration of the natural history of hepatitis C is caused in part by immunosuppression. 2. Risk factors for aggressive recurrence, graft loss, and death are treated acute cellular rejection, methylprednisolone pulse therapy, and use of OKT3. There appears to be no consistent difference between cyclosporine and tacrolimus in their effects on hepatitis C. 3. The benefit of steroid withdrawal, although commonly practiced in transplant recipients with hepatitis C, has not been proven. 4. Mycophenolate mofetil may show synergistic antiviral properties when used with interferon; however, posttransplantation use has not been associated with consistent beneficial or deleterious effects. 5. Effects of other agents, such as sirolimus or interleukin-2-receptor antibodies, have not been adequately defined. Early reports suggest that disease activity may be more aggressive when these agents are constituents of the immunosuppressive regimen.  N. Ref:: 54

 

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[159]

TÍTULO / TITLE:  - Limited dose monoclonal IL-2R antibody induction protocol after primary kidney transplantation.

REVISTA / JOURNAL:  - Am J Transplant 2002 Jul;2(6):568-73.

AUTORES / AUTHORS:  - Ahsan N; Holman MJ; Jarowenko MV; Razzaque MS; Yang HC

INSTITUCIÓN / INSTITUTION:  - Nephrology and Transplant Division, University of Medicine and Dentistry of New Jersey, New Brunswick 08904, USA. ahsanna@umdnj.edu

RESUMEN / SUMMARY:  - This study prospectively compared immunoprophylaxis with a single intraoperative dose (2 mg/kg) of monoclonal interleukin-2 receptor (IL-2R) antibody vs. noninduction in kidney transplant recipients treated with tacrolimus (FK 506), mycophenolate mofetil (MMF) and a prednisone-based immunosuppression regimen. One hundred recipients of first-kidney transplant were enrolled into the study to receive either anti-IL-2R monoclonal antibody, daclizumab (2 mg/kg intraoperatively, limited anti-IL-2R) or no induction (control). Each patient also received oral tacrolimus (dosed to target trough level 10-15 ng/mL), MMF (500 mg bid) and prednisone. The primary efficacy end-point was the incidence of biopsy proven acute rejection during the first 6 months post-transplant. The patients were also followed for 12-month graft function, and graft and patient survival rates. Other than the donor’s age being significantly lower in the control group, both groups were comparable with respect to age, weight, gender, race, human leukocyte antigen (HLA)-DR mismatch, panel reactive antibody (%PRA), cold ischemic time, cytomegalovirus (CMV) status, causes of renal failure, and duration and modes of renal replacement therapy (RRT). During the first 6 months, episodes of first biopsy confirmed acute rejection was 3/50 (6%) in the limited anti-IL-2R group and 8/50 (16%) in the controls (p < 0.05). Twelve-month patient 100/98 (%) and graft survival 100/96 (%) were not statistically different. The group receiving limited anti-IL-2R did not have any adverse reactions. Our study demonstrates that a limited (single) 2 mg/kg immunoprophylaxis dose with monoclonal IL-2R antibody (daclizumab) when combined with tacrolimus/MMF/steroid allows significant reduction in early renal allograft rejection to the single digit level. The therapy with anti-IL-2R antibody is simple and is well tolerated.

 

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[160]

TÍTULO / TITLE:  - The utility of monoclonal antibody therapy in renal transplantation.

REVISTA / JOURNAL:  - Transplant Proc 2002 May;34(3):797-800.

AUTORES / AUTHORS:  - Loertscher R

INSTITUCIÓN / INSTITUTION:  - Division of Transplantation, McGill University Health Centre, Montreal, Quebec, Canada. rolf.loertscher@mcgill.ca  N. Ref:: 37

 

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[161]

TÍTULO / TITLE:  - Tacrolimus in cardiac transplantation: efficacy and safety of a novel dosing protocol.

REVISTA / JOURNAL:  - Transplantation 2002 Oct 27;74(8):1136-41.

      ●● Enlace al texto completo (gratuito o de pago) 1097/01.TP.0000034030.29014.88

AUTORES / AUTHORS:  - Baran DA; Galin I; Sandler D; Segura L; Cheng J; Courtney MC; Correa R; Chan M; Fallon JT; Spielvogel D; Lansman SL; Gass AL

INSTITUCIÓN / INSTITUTION:  - Zena and Michael A. Weiner Cardiovascular Institute, Mt. Sinai Medical Center, Box 1030, New York, NY 10029, USA. David.Baran@mountsinai.org

RESUMEN / SUMMARY:  - BACKGROUND: Although used for more than 20 years, optimal dosing strategies of most immunosuppressants have never been determined. Tacrolimus, one of the newer agents used in solid-organ transplantation, is gaining increasing popularity because of its ability to reverse refractory rejection in cyclosporine-treated patients and its favorable side-effect profile. As with many other immunosuppressive agents, absorption and metabolism vary between individuals, which complicates dosing. METHODS: We hypothesized that a 1-mg dose of tacrolimus may be used to gauge each patient’s metabolism. A novel dosing scheme was evaluated to establish the safety and efficacy of this approach. Outcomes were incidence of renal insufficiency and treatment efficacy as assessed by the rejection grade on the first endomyocardial biopsy. RESULTS: The risk of renal insufficiency was low, with only a 3% rise in creatinine at 7 days posttransplant. The risk of renal insufficiency was highest during the first 3 days of tacrolimus therapy, and the change in tacrolimus level during this time was identified as the single best predictor of renal insufficiency. From days 4 to 7, the rise in tacrolimus level had much less influence on renal function. Ninety-two percent of patients had a low- or intermediate-grade first cardiac biopsy. CONCLUSIONS: It was shown that this conservative initial dosing approach, which guarantees renal safety, is not associated with an increased risk of allograft rejection. We conclude that administration of tacrolimus via a tailored protocol soon after transplantation ensures a safe and effective means of immunosuppression.

 

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[162]

TÍTULO / TITLE:  - Recommendations for the implementation of Neoral C(2) monitoring in clinical practice.

REVISTA / JOURNAL:  - Transplantation 2002 May 15;73(9 Suppl):S19-22.

AUTORES / AUTHORS:  - Cole E; Midtvedt K; Johnston A; Pattison J; O’Grady C

INSTITUCIÓN / INSTITUTION:  - University of Toronto, Toronto General Hospital, 621 University Ave., Toronto, Ontario M5G 2C4, Canada.

 

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[163]

TÍTULO / TITLE:  - Safety and efficacy of TOR inhibitors in pediatric renal transplant recipients.

REVISTA / JOURNAL:  - Am J Kidney Dis 2001 Oct;38(4 Suppl 2):S22-8.

AUTORES / AUTHORS:  - Ettenger RB; Grimm EM

INSTITUCIÓN / INSTITUTION:  - Department of Pediatrics, Mattel Children’s Hospital at UCLA, Los Angeles, CA 90095-1752, USA. Rettenger@mednet.ucla.edu

RESUMEN / SUMMARY:  - Information about the pharmacokinetics, safety, and efficacy of target of rapamycin (TOR) inhibitors, such as sirolimus and everolimus, in pediatric renal transplant recipients is limited. In an ascending single-dose pharmacokinetic study of sirolimus in pediatric dialysis patients, no clinically significant association was observed between patient age and absorption of sirolimus from the gastrointestinal tract. However, young pediatric patients (5 to 11 years of age) exhibited significantly greater apparent oral clearances, suggesting that pediatric patients require slightly higher doses than do adults when adjusted for body weight or surface area. Similarly, in studies performed in pediatric renal transplant recipients, the half-life of sirolimus was shorter and the clearance was greater in younger patients. On the other hand, in single-dose pharmacokinetic studies of everolimus, the apparent clearance was reduced in pediatric renal transplant recipients compared with clearance in adults. This reduced clearance was attributed to a smaller apparent volume of distribution in pediatric patients, rather than to a difference in terminal half-life. This suggested that, although the adult 12-hour dosing interval was appropriate for pediatric patients, they would require reduced dosing based on body size compared with adults. In a large trial (N = 719) of sirolimus versus azathioprine in combination with cyclosporine microemulsion and prednisone, 6 pediatric patients (13 to 18 years of age) received sirolimus at 2 mg/d, 3 received sirolimus at 5 mg/d, and 3 received azathioprine. Seven of the nine patients who received sirolimus experienced no rejection episodes. Six infectious episodes occurred in the 6 patients receiving sirolimus at 2 mg/d, 10 episodes occurred in the 3 patients receiving sirolimus at 5 mg/d, and 8 episodes occurred in the 3 patients receiving azathioprine. At 6 months after transplantation, renal function was similar in all 3 groups, although there was a statistically nonsignificant increase in the group receiving sirolimus at 5 mg/d. The mean cholesterol and triglyceride levels were generally comparable in all 3 groups. TOR inhibitors are promising agents for the prevention of graft rejection in pediatric renal transplant recipients, but more pharmacokinetic data are required to assess the optimal dosing regimens in this population. In addition, further data are needed on the efficacy and safety of TOR inhibitors in combination with other agents in pediatric transplantation recipients to best assess the role of TOR inhibition in corticosteroid and/or calcineurin inhibitor-sparing regimens.  N. Ref:: 13

 

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[164]

TÍTULO / TITLE:  - Low-intensity hematopoietic stem-cell transplantation across human leucocyte antigen barriers in dyskeratosis congenita.

REVISTA / JOURNAL:  - Bone Marrow Transplant 2003 May;31(10):847-50.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj.bmt.1703931

AUTORES / AUTHORS:  - Dror Y; Freedman MH; Leaker M; Verbeek J; Armstrong CA; Saunders FE; Doyle JJ

INSTITUCIÓN / INSTITUTION:  - Marrow Failure and Myelodysplasia Programme, Division of Haematology and Oncology, Department of Paediatrics, The Hospital for Sick Children and the University of Toronto, Toronto, Ontario, Canada.

RESUMEN / SUMMARY:  - Since the results of conventional hematopoietic stem-cell transplantation (HSCT) for patients with dyskeratosis congenita (DC) are poor owing to the high incidence of transplant-related complications, we explored the use of a low-intensity HSCT regimen. We report two children with DC with severe cytopenia, who underwent successful HSCT from a matched unrelated donor after conditioning with fludarabine, cyclophosphamide, and antithymocyte globulin. Graft-versus-host-disease (GVHD) prophylaxis consisted of corticosteroids and cyclosporin A. The regimen was well tolerated, no significant transplant-related complications were observed, and engraftment was rapid and complete. At 15 and 16 months after HSCT, the children were fully engrafted, in excellent clinical condition, full-donor chimerism, and no signs of GVHD. We conclude that a low-intensity regimen is sufficient to induce durable engraftment using matched unrelated donor HSCT in DC patients, with minimal 1-year transplant-related toxicity. Longer follow-up will determine whether this regimen also reduces long-term toxicity.  N. Ref:: 35

 

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[165]

TÍTULO / TITLE:  - Tacrolimus versus cyclosporine after lung transplantation: a prospective, open, randomized two-center trial comparing two different immunosuppressive protocols.

REVISTA / JOURNAL:  - J Heart Lung Transplant 2001 May;20(5):511-7.

AUTORES / AUTHORS:  - Treede H; Klepetko W; Reichenspurner H; Zuckermann A; Meiser B; Birsan T; Wisser W; Reichert B

INSTITUCIÓN / INSTITUTION:  - Ludwig-Maximilians-University, Munich, Germany.

RESUMEN / SUMMARY:  - BACKGROUND: The need for better immunosuppressive protocols after lung transplantation led us to investigate tacrolimus (Tac) in combination with mycophenolate mofetil (MMF) and steroids or cyclosporine (CsA) in combination with MMF and steroids in a prospective, open, randomized trial after lung transplantation. METHODS: Between September 1997 and April 1999, 50 lung transplant recipients were randomized to receive either Tac (n = 26) or CsA (n = 24) in combination with MMF and steroids. All patients underwent induction therapy with rabbit antithymocyte globulin (ATG) for 3 days. Freedom from acute rejection (AR), patient survival, infection episodes, and side effects were monitored. RESULTS: There was no difference in patient demographics between the two groups. Six-month and 1-year survival was similar (84.6% and 73.1% in the Tac group vs 83.3% and 79.2% in the CsA group). Freedom from AR at 6 months and 1 year after lung transplantation was slightly higher in the Tac group (57.7% and 50% vs 45.8% and 33.3%, p = not significant [n.s.]), whereas the number of treated rejection episodes per 100 patient days in the Tac group was significantly lower (0.225 vs 0.426, p < .05). Four patients in the CsA group had to be switched to Tac. Two patients in the CsA group had to be retransplanted. Incidence of infections was similar in both groups with a trend toward more fungal infections in the Tac group (n = 7 vs n = 1, p = n.s.). CONCLUSIONS: The combination of Tac and MMF seems to have slightly higher immunosuppressive potential compared with CsA and MMF. The effectiveness of Tac as a rescue agent is not paralleled with undue signs of overimmunosuppression.

 

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[166]

TÍTULO / TITLE:  - Donor cell-derived acute myeloid leukemia developing 14 months after matched unrelated bone marrow transplantation for chronic myeloid leukemia.

REVISTA / JOURNAL:  - Bone Marrow Transplant 2001 Oct;28(7):705-7.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj/bmt/1703225

AUTORES / AUTHORS:  - Hambach L; Eder M; Dammann E; Battmer K; Stucki A; Heil G; Ganser A; Hertenstein B

INSTITUCIÓN / INSTITUTION:  - Department of Hematology and Oncology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

RESUMEN / SUMMARY:  - We report a patient with Ph-positive CML who developed a Ph-negative AML in donor cells 14 months after BMT from an HLA-identical male unrelated donor. The Ph translocation could not be detected by either conventional cytogenetics, FISH or RT-PCR analysis excluding relapse of CML in myeloid blast crisis. Chimerism studies were performed by variable number of tandem repeats (VNTR) analysis. These revealed donor-type hematopoiesis in both unseparated mononuclear cells and CD34+ selected blasts proving the leukemia to be of donor origin. The patient received three cycles of polychemotherapy with mitoxantrone, topotecan and ara-c resulting in CR after the first treatment cycle and reconstitution with donor hematopoiesis. A second transplant from a female alternative matched unrelated donor was performed after conditioning with fludarabine and 200 cGy TBI and was well tolerated. Nine months after the second transplant the patient is at home and in CR. T cell chimerism was studied by sex chromosome analysis and revealed complete female donor chimerism.  N. Ref:: 13

 

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[167]

TÍTULO / TITLE:  - Antioxidant nutrients protect against cyclosporine A nephrotoxicity.

REVISTA / JOURNAL:  - Toxicology 2003 Jul 15;189(1-2):99-111.

AUTORES / AUTHORS:  - Parra Cid T; Conejo Garcia JR; Carballo Alvarez F; de Arriba G

INSTITUCIÓN / INSTITUTION:  - Unidad de Investigacion, Hospital Universitario de Guadalajara, C/Donante de Sangre s/n, 19.002 Guadalajara, España. tparracid@hotmail.com

RESUMEN / SUMMARY:  - The immunosuppressive drug cyclosporine A (CsA) has been successfully used in several diseases with immunological basis and in transplant patients. Nephrotoxicity is the main secondary effect of CsA treatment. Although the mechanisms of nephrotoxitity are not completely defined, there is evidence that suggests the role of reactive oxygen species (ROS) in its pathogenesis. It has been demonstrated in numerous in vivo and in vitro experiments that CsA induced renal failure and increased the synthesis of ROS, thromboxane (TX) and lipid peroxidation products in the kidney. Furthermore, CsA modified the expression and activity of several renal enzymes (ciclooxygenase, superoxide-dismutase, catalase and glutathione-peroxidase). Antioxidant nutrients (e.g. Vitamins E and C) can neutralize some of the effects that CsA produced in the kidney. Thus, Vit E inhibited the synthesis of ROS and TX and the lipid peroxidation process induced by CsA in kidney structures. Antioxidants can also improve renal function and histological damage produced by CsA administration. Although there are few data in humans treated with CsA, the possibility exists that antioxidants can also neutralize CsA nephrotoxicity and LDL oxidation. Thus, antioxidant nutrients could have a therapeutic role in transplant patients treated with CsA.  N. Ref:: 79

 

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[168]

TÍTULO / TITLE:  - Vascular and cellular mechanisms of chronic renal allograft dysfunction.

REVISTA / JOURNAL:  - Transplantation 2001 Jun 15;71(11 Suppl):SS37-41.

AUTORES / AUTHORS:  - Morris RE

INSTITUCIÓN / INSTITUTION:  - Stanford University School of Medicine, California, United States.  N. Ref:: 29

 

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[169]

TÍTULO / TITLE:  - Role of prostanoids and endothelins in the prevention of cyclosporine-induced nephrotoxicity.

REVISTA / JOURNAL:  - Prostaglandins Leukot Essent Fatty Acids 2001 Apr-May;64(4-5):231-9.

      ●● Enlace al texto completo (gratuito o de pago) 1054/plef.2001.0265

AUTORES / AUTHORS:  - Darlametsos IE; Varonos DD

INSTITUCIÓN / INSTITUTION:  - Centre Franco-Hellenique de Recherches Biomedicales, Nikolaos Papanikolaou, Corporation of the Municipality Agrinion, Agrinion, 30100, Greece. darlamet@otenet.gr

RESUMEN / SUMMARY:  - Cyclosporine A nephrotoxicity includes both functional toxicity and histological changes, whose seriousness is dependent upon the dose and the duration of the drug administration. Several vasoactive agents have been found to be implicated in cyclosporine induced nephrotoxicity, among which prostanoids and endothelins are the most important. In previous studies we were able to prevent the early stage (7 days) of cyclosporine (37.4 micromol [45 mg]/kg/day) induced nephrotoxicity in rats either by the administration, i) of OKY-046, a thromboxane A(2)synthase inhibitor, ii) of ketanserine, an antagonist of S(2)serotonergic, a(1)adrenergic, and H(1)histaminergic receptors and iii) of nifedipine, a calcium channel blocker, or by diet supplementation either with evening primrose oil or fish oil. All these protective agents elevated ratios of excreted renal prostanoid vasodilators (prostaglandins E(2), 6ketoF(1 alpha)) to vasoconstrictor (thromboxane B(2)), a ratio which was decreased by the administration of cyclosporine alone. Nifedipine averted the cyclosporine induced increase of urinary endothelin-1 release. All protections were associated with the reinstatement of glomerular filtration rate forwards normal levels whereas renal damage defence, consisting of a decrease of the cyclosporine induced vacuolizations, was variable. Ketanserine and evening primrose oil were the only agents which prevented the animal body weight loss. These data suggest that prostanoids and endothelin-1 may mediate functional toxicity while thromboxane A(2)is involved the morphological changes too, provoked in the early stage of cyclosporine treatment. However, other nephrotoxic factors and additional mechanisms could also be implicated in the cyclosporine induced nephrotoxicity.  N. Ref:: 91

 

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[170]

TÍTULO / TITLE:  - Role of chiral chromatography in therapeutic drug monitoring and in clinical and forensic toxicology.

REVISTA / JOURNAL:  - Ther Drug Monit 2002 Apr;24(2):290-6.

AUTORES / AUTHORS:  - Williams ML; Wainer IW

INSTITUCIÓN / INSTITUTION:  - Department of Oncology, Leicester University, Leicester, United Kingdom.

RESUMEN / SUMMARY:  - Advances in chiral chromatographic separations have given pharmacologists and toxicologists the tools to examine unexpected clinical results involving chiral drugs. The ability to unravel complex phenomena associated with drug transport and drug metabolism is presented in this manuscript. The relation between the chirality of the drug mefloquine and the intracellular concentrations of the drug cyclosporine is illustrated by examining the effect of the enantiomers of mefloquine on the transport activity of P-glycoprotein (Pgp). These studies were conducted using a liquid chromatographic column containing immobilized Pgp. The results demonstrated that (+)-mefloquine competitively displaced the Pgp substrate cyclosporine whereas (-)-mefloquine had no effect on cyclosporine-Pgp binding. The data suggest that cyclosporine cellular and CNS concentrations can be increased through the concomitant administration of (+)-mefloquine. The use of chirality in clinical and forensic situations is also illustrated by the metabolism of the enantiomers of ketamine (KET). The plasma concentrations of (+)-KET and (-)-KET and the norketamine metabolites (+)-NK and (-)-NK were measured in rat plasma using enantioselective gas chromatography. The separations were accomplished using a gas chromatography chiral stationary phase based on beta-cyclodextrin. The pharmacokinetic profiles of (+)-, (-)-KET and (+)-, (-)-NK were determined in control and protein-calorie malnourished (PCM) rats to determine the effect of PCM on ketamine metabolism and clearance. The results indicate that PCM produced a significant and stereoselective decrease in KET and NK metabolism. The data suggest that the effects of environmental factors (smoking, alcohol use, diet) and drug interactions (coadministered agents) can be measured using the changes in stereochemical metabolic and pharmacokinetic patterns of KET and similar drugs.  N. Ref:: 33

 

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[171]

TÍTULO / TITLE:  - Ten years of sirolimus therapy in orthotopic liver transplant recipients.

REVISTA / JOURNAL:  - Transplant Proc 2003 May;35(3 Suppl):209S-216S.

AUTORES / AUTHORS:  - Neff GW; Montalbano M; Tzakis AG

INSTITUCIÓN / INSTITUTION:  - University of Miami, Miami, Florida, USA.

RESUMEN / SUMMARY:  - BACKGROUND: Sirolimus therapy has been used in orthotopic liver transplant (OLT) recipients diagnosed with a variety of diseases; chronic graft rejection (CR), calcineurin associated renal toxicity, preemptive immune suppression, calcineurin related neurotoxicity, preemptive therapy in transplant recipients with history of hepatocellular carcinoma, and steroid resistant allograft rejection. METHODS: A search for the medical literature and experiences involving sirolimus was done. RESULTS: Several animal and human reports evaluating the use sirolimus in liver transplant recipients are found and discussed. CONCLUSION: Sirolimus has been used for multitude of indications, primarily based on anecdotal experiences. However, reports of sirolimus related side effects have decreased the transplant communities’ enthusiasm towards promoting this agent as a safe immune suppression agent.  N. Ref:: 92

 

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[172]

TÍTULO / TITLE:  - Suggested guidelines for the use of tacrolimus in cardiac transplant recipients.

REVISTA / JOURNAL:  - J Heart Lung Transplant 2001 Jul;20(7):734-8.

AUTORES / AUTHORS:  - Taylor DO; Barr ML; Meiser BM; Pham SM; Mentzer RM; Gass AL

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, Division of Cardiology, University of Utah, Salt Lake City, Utah 84132, USA.  N. Ref:: 11

 

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[173]

TÍTULO / TITLE:  - Combined intravenous pulse methylprednisolone and oral cyclosporine A in the treatment of corneal graft rejection: 5-year experience.

REVISTA / JOURNAL:  - Eye 2002 May;16(3):304-8.

      ●● Enlace al texto completo (gratuito o de pago) 1038/sj/eye/6700144

AUTORES / AUTHORS:  - Young AL; Rao SK; Cheng LL; Wong AK; Leung AT; Lam DS

INSTITUCIÓN / INSTITUTION:  - Department of Ophthalmology & Visual Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong.

RESUMEN / SUMMARY:  - PURPOSE: To report the mid-term results of a treatment strategy using topical steroids, intravenous pulse methyl prednisolone and oral cyclosporine A (CSA) for the treatment of acute corneal graft rejection. METHODS: Noncomparative, interventional case series. Treatment of corneal graft rejection included 1% prednisolone eye drops, intravenous infusion of 500 mg methyl prednisolone, and oral CSA in two regimens—standard dose was 15 mg/kg/day for 2 days, 7.5 mg/kg/day for 2 days, then adjusted to maintain trough blood levels of 100-200 microg/l; low dose was 2 mg/kg/day with no loading dose. RESULTS: Outcome in 34 eyes of 34 patients (21 M;13 F) aged 60 +/- 17.7 years (range 9-83 years), who presented after an average duration of 6.6 +/- 6.3 days (range 0-30 days) following acute corneal graft rejection, are reported. Twenty-five patients received standard dose CSA while nine patients received the low dose regimen. Mean duration of treatment before reversal of graft rejection was 13.6 +/- 12.1 days (range 3-54 days). Treatment was successful in reversing the graft rejection in 32/34 (94%) eyes. Irreversible graft failure occurred in one eye in each group. During a mean follow-up period of 19.2 +/- 16.7 months (range 1-55 months), further episodes of graft rejection were seen in 1/32 (3%) eyes. Complications due to treatment included: duodenal ulcer in one patient that responded to medical treatment, and transient elevation in serum creatinine levels in three patients, which returned to normal after decrease in dosage or cessation of oral CSA. CONCLUSION: Our 5-year experience with the use of oral CSA in the treatment of acute corneal graft rejection has shown this treatment approach to be safe and effective in reversing the rejection process. This approach may also protect the graft from subsequent episodes of allograft rejection. A randomised controlled trial to further delineate the role of CSA in reversing acute graft rejection seems warranted.  N. Ref:: 17

 

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[174]

TÍTULO / TITLE:  - Diffusion-weighted MR imaging of posterior reversible leukoencephalopathy syndrome: a pictorial essay.

REVISTA / JOURNAL:  - Clin Imaging 2003 Sep-Oct;27(5):307-15.

AUTORES / AUTHORS:  - Kinoshita T; Moritani T; Shrier DA; Hiwatashi A; Wang HZ; Numaguchi Y; Westesson PL

INSTITUCIÓN / INSTITUTION:  - Department of Radiology, Division of Radiology, University of Rochester Medical Center, 601 Elmwood Avenue Box 648, Rochester, NY 14642, USA. kino@grape.med.tottori-u.ac.jp

RESUMEN / SUMMARY:  - Posterior reversible leukoencephalopathy syndrome is characterized by reversible white matter lesions. However, ischemic injury with irreversible damage may occur. This pictorial essay illustrates MR features associated with posterior reversible leukoencephalopathy syndrome. We will emphasize the role of diffusion-weighted imaging for the discrimination of irreversible ischemic injury from reversible vasogenic edema.  N. Ref:: 9

 

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[175]

TÍTULO / TITLE:  - New therapeutic modalities in the treatment of graft-versus-host disease.

REVISTA / JOURNAL:  - Crit Rev Oncol Hematol 2001 May;38(2):129-38.

AUTORES / AUTHORS:  - Basara N; Kiehl MG; Fauser AA

INSTITUCIÓN / INSTITUTION:  - Clinic of Bone Marrow Transplantation and Haematology/Oncology, Dr Ottmar-Kohler Str. 2, 55743, Idar Oberstein, Germany. office@bmt-center-io.com

RESUMEN / SUMMARY:  - Acute and chronic GvHD are still a major concern in allogeneic hematopoietic stem cell transplantation, still contributing substantially to morbidity and mortality in this therapeutic procedure. Over the past decade, many advances have been made with regard to the prevention and treatment of GvHD using various drugs such as cyclosporine A, FK506, mycophenolate mofetil and/or monoclonal IL-2 receptor antagonists. Despite these measurements with regard to the prevention of acute GvHD, it is very difficult to treat these clinical conditions successfully. However, if patients do not experience any GvHD often the desired effect of graft versus leukemia (GvL) remains absent increasing the probability of a relapse, in particular, in patients transplanted, which are considered at higher risk for relapse. At the present time, new strategies in the prevention of acute GvHD are in progress in particular the use of genetic manipulated donor T cells expressing suicide genes. Further clinical and laboratory studies are required in order to improve the prevention and, in particular, the therapy of established GvHD.  N. Ref:: 84

 

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[176]

TÍTULO / TITLE:  - New strategies to reduce nephrotoxicity.

REVISTA / JOURNAL:  - Transplantation 2001 Dec 27;72(12 Suppl):S99-104.

AUTORES / AUTHORS:  - Kreis H

RESUMEN / SUMMARY:  - Since the introduction of cyclosporine, CNIs have formed the basis of immunosuppressive therapy in renal transplantation. The propensity of these agents to ultimately damage the very organs they were intended to protect was always recognized, but largely ignored due to their impressive ability to improve short-term outcomes. With the availability of equally powerful new immunosuppressive agents devoid of major nephrotoxicity, the irony of this situation has become all too apparent, and investigators are beginning to reevaluate the role of CNIs in renal transplantation. In this paper, we looked at strategies using MMF or sirolimus to reduce, withdraw, or replace CNIs in renal transplantation. Although MMF has proved effective in combination with CNIs, particularly in reducing acute rejection rates, its use as base therapy to allow CNI therapy to be withdrawn or eliminated is questionable. On the basis of initial trials, sirolimus holds promise for use as base therapy. To date, it is probably the only agent used in renal transplantation that provides immunosuppression comparable to cyclosporine or tacrolimus, which may someday allow sirolimus to replace. CNIs or allow early withdrawal of CNI therapy. Further study is needed to better clarify the role of sirolimus in improving long-term renal transplantation outcomes.  N. Ref:: 61

 

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[177]

TÍTULO / TITLE:  - Monitoring of cellular resistance to cancer chemotherapy.

REVISTA / JOURNAL:  - Hematol Oncol Clin North Am 2002 Apr;16(2):357-72, vi.

AUTORES / AUTHORS:  - Krishan A; Arya P

INSTITUCIÓN / INSTITUTION:  - Radiation Oncology Department, University of Miami Medical School, Division of Experimental Therapeutics (R-71), P.O. Box 01690, Miami, FL 33101, USA. akrishan@med.miami.edu

RESUMEN / SUMMARY:  - Cellular resistance to a broad spectrum of natural products used as antitumor drugs is believed to be a major cause for the failure of chemotherapy. Flow cytometry has been used for monitoring the expression of drug resistance markers, determining accumulation of fluorescent drugs, and for screening of drugs that enhance chemosensitivity by blocking efflux and enhancing drug retention. This article reviews recent developments in our understanding of the multiple drug resistance phenotype and the use of flow cytometry for the study of drug efflux and its modulation in human tumor cells.  N. Ref:: 77

 

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[178]

TÍTULO / TITLE:  - Sirolimus therapy in cardiac transplantation.

REVISTA / JOURNAL:  - Transplant Proc 2003 May;35(3 Suppl):171S-176S.

AUTORES / AUTHORS:  - Radovancevic B; Vrtovec B

INSTITUCIÓN / INSTITUTION:  - Texas Heart Institute at St. Luke’s Episcopal Hospital, Houston, Texas, USA.

RESUMEN / SUMMARY:  - Rapamycin powerfully inhibits the progression of antigen-activated T cells through the cell cycle. In animal heart transplantation models, rapamycin therapy has been associated with profound immunosuppressive effects on host humoral and cellular responses. In consequence, further studies have been conducted to evaluate the efficiency of rapamycin in preventing acute heart allograft rejection, treating refractory acute heart allograft rejection, inducing transplantation tolerance, and preventing and treating transplant coronary artery disease. The results of these studies indicated that rapamycin can effectively prevent acute graft rejection and inhibit refractory acute graft rejection in heart transplant recipients by exerting potent immunosuppressive and antiproliferative effects without adversely affecting renal function. This supports the use of rapamycin therapy in heart transplant recipients, especially in those with renal dysfunction, for whom treatment with calcineurin inhibitors is contraindicated. Rapamycin may also halt and even reverse the progression of cardiac allograft vasculopathy, which warrants further clinical trials in humans. Finally, rapamycin may be able to induce transplantation tolerance, thus making it one of the most promising modalities for improving the long-term survival of heart transplant recipients.  N. Ref:: 41

 

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[179]

TÍTULO / TITLE:  - Pimecrolimus (Elidel, SDZ ASM 981)--preclinical pharmacologic profile and skin selectivity.

REVISTA / JOURNAL:  - Semin Cutan Med Surg 2001 Dec;20(4):233-41.

AUTORES / AUTHORS:  - Stuetz A; Grassberger M; Meingassner JG

INSTITUCIÓN / INSTITUTION:  - Novartis Research Institute, Vienna, Austria.

RESUMEN / SUMMARY:  - The ascomycin macrolactam derivative pimecrolimus (Elidel, SDZ ASM 981; Novartis Pharma AG, Basel Switzerland) is a cell-selective inhibitor of inflammatory cytokines specifically developed for the treatment of inflammatory skin diseases, such as atopic dermatitis, allergic contact dermatitis, irritant contact dermatitis, and plaque-type psoriasis. It inhibits the production of inflammatory cytokines in T cells and mast cells and prevents the release of preformed inflammatory mediators from mast cells. Topically administered pimecrolimus is as effective as the high-potency corticosteroid clobetasol-17-propionate in a pig model of allergic contact dermatitis (ACD). Unlike clobetasol, however, it does not cause skin atrophy. Given orally, pimecrolimus is as potent or superior to tacrolimus (FK 506) in treating ACD in mice and rats. Pimecrolimus also effectively reduces skin inflammation and pruritus in hypomagnesemic hairless rats, a model that mimics acute signs of atopic dermatitis. Pimecrolimus shows only a low potential to impair systemic immune responses when compared with tacrolimus as shown in rats in (1) the localized graft-versus-host reaction, (2) the antibody formation to sheep red blood cells, and (3) kidney transplantation. Pimecrolimus permeates through pig skin in vitro at a 10-times lower rate than tacrolimus, indicating a lower potential for percutaneous absorption in vivo. The data suggest that pimecrolimus combines high anti-inflammatory activity in the skin with a low potential to impair systemic immune reactions.  N. Ref:: 31

 

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[180]

TÍTULO / TITLE:  - Molecular actions of sirolimus: sirolimus and mTor.

REVISTA / JOURNAL:  - Transplant Proc 2003 May;35(3 Suppl):227S-230S.

AUTORES / AUTHORS:  - Kirken RA; Wang YL

INSTITUCIÓN / INSTITUTION:  - Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, Houston, Texas 77030, USA. robert.a.kirken@uth.tmc.edu

RESUMEN / SUMMARY:  - Recent therapeutic strategies to combat organ allograft rejection have focused on T-cell signaling pathways and the molecules that comprise them. The macrolide antibiotic produced by the bacterium Streptomyces hygroscopicus, known as sirolimus or rapamycin, has shown great therapeutic potential in the transplant setting. Sirolimus alone or in combination with other immunosuppressive agents can block acute rejection, chronic graft destruction, and promote permanent allograft acceptance. Sirolimus targets a unique serine-threonine kinase, mammalian target of rapamycin (mTor), which plays a key role in mitogenic and nutritional cells signals. Within T cells, mTor regulates a number of proteins likely dependent on T cell growth factors such as interleukin 2. This review is focused on the molecular mechanisms by which mTor may regulate T-cell signaling cascades and affect T-cell responsiveness, and how sirolimus likely uncouples this activity.  N. Ref:: 32

 

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[181]

TÍTULO / TITLE:  - Treatment of patients with chronic idiopathic urticaria.

REVISTA / JOURNAL:  - Clin Rev Allergy Immunol 2002 Oct;23(2):233-41.

AUTORES / AUTHORS:  - Stanaland BE

INSTITUCIÓN / INSTITUTION:  - Division of Allergy and Immunology, University of South Florida Health Sciences Center, USA.

RESUMEN / SUMMARY:  - Treatment of patients with chronic idiopathic urticaria (CIU) involves reducing symptoms with the least invasive therapy and carefully balancing risk and benefit. The mainstay of therapy is the use of antihistamines with or without the use of intermittent pulses of corticosteroids. Alternative therapies to chronic corticosteroids include leukotriene antagonists, plasma-phoresis, dapsone, stanazolol, hydroxychloroquine, methotrexate, cyclosporin, tacrolimus, and warfarin. A practical approach to CIU bases treatment and severity on the patients’ previous response to therapy. Therapy goals are to reduce symptoms until spontaneous resolution occurs. Management of CIU patients can be both frustrating and rewarding.  N. Ref:: 34

 

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[182]

TÍTULO / TITLE:  - Long-term kidney transplant survival.

REVISTA / JOURNAL:  - Am J Kidney Dis 2001 Dec;38(6 Suppl 6):S44-50.

AUTORES / AUTHORS:  - Hariharan S

INSTITUCIÓN / INSTITUTION:  - Froedert Memorial Hospital, Medical College of Wisconsin, Milwaukee, WI 53226, USA. hari@mcw.edu

RESUMEN / SUMMARY:  - With improvements in short-term kidney graft survival, focus has shifted towards long-term survival. There has also been a substantial improvement in long-term survival as measured by kidney half-life. Long-term graft failure is secondary to chronic allograft nephropathy (CAN), recurrent disease, and death with a functioning graft. CAN is secondary to a combination of chronic rejection, chronic cyclosporine toxicity, and/or donor kidney disease. Risk factors for chronic rejection have been attributed to both immunological and nonimmunological causes. With a marked reduction in acute rejection rates-an important risk factor for CAN-there is a substantial improvement in kidney half-life. There are still nonimmunological factors, such as donor age, that adversely affect long-term graft survival. In addition, African-American recipients continue to have a shorter graft half-life. Recurrent disease is becoming an important cause of late graft failure. Despite the introduction of various potent immunosuppressive agents, there has been little or no impact on the prevalence as well as progression of recurrent diasease. With the reduction of acute rejection rates and improved short- and long-term graft survival, further improvements of long-term graft survival will be an important focus in the 21st century.  N. Ref:: 45

 

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[183]

TÍTULO / TITLE:  - Intestinal graft-versus-host disease: mechanisms and management.

REVISTA / JOURNAL:  - Drugs 2003;63(1):1-15.

AUTORES / AUTHORS:  - Takatsuka H; Iwasaki T; Okamoto T; Kakishita E

INSTITUCIÓN / INSTITUTION:  - Second Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan. hematol@hyo-med.ac.jp

RESUMEN / SUMMARY:  - Allogeneic haematopoietic stem cell transplantation remains the treatment of choice for a number of malignancies. However, graft-versus-host disease (GVHD) has long been regarded as a serious complication of this procedure. Although GVHD may affect any organ, intestinal GVHD is particularly important because of its frequency, severity and impact on the general condition of the patient. Recent studies have led to progressive elucidation of the mechanism of GVHD. Donor T cells are critical for the induction of GVHD, because depletion of T cells from bone marrow grafts effectively prevents GVHD but also results in an increase of leukaemia relapse. It has been shown that the gastrointestinal tract plays a major role in the amplification of systemic disease because gastrointestinal damage increases the translocation of endotoxins, which promotes further inflammation and additional gastrointestinal damage. Consequently, the management of intestinal GVHD (and the intestine itself) is a subject that should be highlighted. In this article, approaches to the prevention of intestinal GVHD are discussed after being classified into three categories: regimens in common clinical use, regimens under investigation and original regimens used at our hospital. The standard regimen that is used most widely for prevention of GVHD is cyclosporin plus short-term methotrexate. Corticosteroids can be added to this regimen but careful consideration of the adverse effects of these hormones should be considered. Tacrolimus is a newer, more potent alternative to cyclosporin. T-cell depletion (TCD) after transplantation has been shown to prevent acute GVHD, however, the survival benefit of TCD has not been as great as expected. Mycophenolate mofetil can be useful for the treatment of acute GVHD as part of combination therapy. Regimens currently under investigation in animal experiments include suppression of inflammatory cytokines and inhibition of T-cell activation, and, specifically at our institution, hepatocyte growth factor gene therapy. The evidence-based therapy used at our institution includes systemic antibacterial therapy (including eradication of intestinal bacteria) to prevent the intestinal translocation of lipopolysaccharide and avoid the subsequent increase of various inflammatory cytokines. In addition, because of the similarities between intestinal GVHD and ulcerative colitis, sulfasalazine, betamethasone enemas and eicosapentaenoic acid have been used to treat intestinal GVHD in some patients.  N. Ref:: 125

 

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[184]

TÍTULO / TITLE:  - The mosaic of immunosuppressive drugs.

REVISTA / JOURNAL:  - Mol Immunol 2003 Jul;39(17-18):1073-7.

AUTORES / AUTHORS:  - Masri MA

INSTITUCIÓN / INSTITUTION:  - Rizk Hospital, P.O Box 11-3288, Beirut, Lebanon. marwan.masri@mysalima.com

RESUMEN / SUMMARY:  - Graft rejections as well as tolerance are true representation of the specificity, sophistication and redundancy of an elegantly and meticulously designed immune system. Tolerance is in a way similar to the process of self-recognition where lymphoid clones, during development, baring self-reactive receptor are eliminated or rendered in active by “clonal deletion” leading to a state of accommodation and acceptance (anergic). On the other hand, both acute and chronic rejections are manifestation of the purpose of existence of the immune system, which is to defend the host against foreign invaders. Thus, in order to treat (control) graft rejection it is necessary to determine and understand the steps leading to recognition, stimulation, activation, and amplification of the immune system. The first step leading to the initiation of the immune system cascade is recognition. Which can either be direct where donor antigens of the major histocompatibility complex (MHC) expressed on the donor cells (passenger leukocytes) or tissues are recognised by the host immune system. The direct recognition pathway initiates acute graft rejection. Alternatively processed donor MHC peptides presented by the recipient antigen presenting cells (APC) initiate the indirect pathway of immune response, which is as important as the direct recognition especially in chronic rejection. Recognition is followed by the ligation of a series of adhesion molecules starting with an antigen to its specific T-cell receptor (TCR)/cluster of differentiation (CD) complex, expressed on the surface of the T cell. In order for the activation to precede additional costimulatory signals, such as ligation of the CD28/B7, CD4/HLA class II and CD/HLA class I antigens are required. The activation process is accompanied by an increase of cytokines production such as interleukin (IL)-2, IL-12, interferon (INF) and tumour necrosis factor (TNF) by the primed T cell. The complexity and the polymorphic nature of the immune system have necessitated designing agents that inhibit the immune system at different levels. Cyclosporine and Tacrolimus, collectively known as calcineurin inhibitors, seems to act on the IL-2 by inhibiting its production thus leading to a decrease in the proliferation of the activated lymphocyte. Rapamycin, which is similar to Tacrolimus, inhibits graft rejection by blocking IL-2 activation and phosphorylation of 70 S6 kinase thus inhibiting the progression of T-cell from G to S phase. While Cellcept (MMF) reduce the proliferation of T cell by inhibiting purine synthesis and by its action on ionosine monophosphate dehydrogenase. Anti-lymphocyte antibodies (ATG) deplete circulating lymphocytes while selective monoclonal antibodies are directed against IL-2 receptor thus reducing the rate of proliferation of activated T cells. Recently, antibodies to the CD40/CD40 ligand have been shown to induce long-term graft survival with the inhibition of the Th1 cytokines (INF), IL-2 and IL-12 and upregulating the Th2 cytokines IL-4 and IL-10. Lastly graft rejection can be reduced by blockade of the B7/CD28 costimulation pathway with the fusion protein CTLA-4Ig. With the availability of such potent and diverse agents it is now possible to develop multi drug regiments that can depress the immune system at the different steps of the activation cascade, with minimal side effects, thus improving graft and patient survival rates.  N. Ref:: 73

 

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[185]

TÍTULO / TITLE:  - Prevention strategies for type 1 diabetes mellitus: current status and future directions.

REVISTA / JOURNAL:  - BioDrugs 2003;17(1):39-64.

AUTORES / AUTHORS:  - Winter WE; Schatz D

INSTITUCIÓN / INSTITUTION:  - Department of Pathology, University of Florida, Gainesville, Florida 32610, USA. winter@pathology.ufl.edu

RESUMEN / SUMMARY:  - Type 1 diabetes mellitus affects about 1 in 300 people in North America and Europe. Epidemiological studies indicate that the incidence and thus prevalence of type 1 diabetes is rising worldwide. Intervention in autoimmune type 1a diabetes could occur at the time of diagnosis or, preferably, prior to clinical presentation during the ‘prediabetic’ period (e.g. prevention). Prediabetes is best recognised by the detection of islet autoantibodies in the serum. Promising intervention strategies include monoclonal antibody therapies (e.g. anti-CD3, anti-CD25, anti-CD52 or anti-CD20 monoclonal antibodies), immunosuppression (e.g. calcineurin inhibitors, B7 blockade, glucocorticoids, sirolimus (rapamycin), azathioprine or mycophenolate mofetil), immunomodulatory therapies (e.g. plasmapheresis, intravenous immunoglobulin, cytokine administration, adoptive cellular gene therapy) and tolerisation interventions (e.g. autoantigen administration or avoidance, altered peptide ligand or peptide-based therapies). To date, islet and pancreas transplantation have essentially been reserved for patients with long-standing diabetes who have complications and are also in need of a concurrent kidney transplant. None of the therapies attempted to date has produced long-term remissions in new-onset type 1 diabetes patients and no therapies have been shown to prevent the disease. Nevertheless, with advances in our understanding of basic immunology and the cellular and molecular mechanisms of tolerance induction and maintenance, successful intervention therapies will be developed. The balance between safety and efficacy is critical. Higher rates of adverse events might be more tolerable in new-onset type 1 diabetes patients if the therapy is extremely effective at inducing a permanent remission. However, therapies must not harm the beta-cells themselves or any organ system that is a potential target of diabetes complications, such as the nervous system, retina, cardiovascular system or kidney. In the treatment of prediabetes, successful therapies should provide a level of safety similar to that of currently used vaccines and a high level of efficacy.  N. Ref:: 244

 

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[186]

TÍTULO / TITLE:  - Tailoring immunosuppressive therapy based on donor and recipient risk factors.

REVISTA / JOURNAL:  - Transplant Proc 2001 May;33(3):2207-11.

AUTORES / AUTHORS:  - First MR

INSTITUCIÓN / INSTITUTION:  - University of Cincinnati Medical Center, Cincinnati, Ohio 45267-0585, USA.  N. Ref:: 35

 

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[187]

TÍTULO / TITLE:  - Cytokine and immunosuppressive therapies of type 1 diabetes mellitus.

REVISTA / JOURNAL:  - Endocrinol Metab Clin North Am 2002 Jun;31(2):477-95.

AUTORES / AUTHORS:  - Gottlieb PA; Hayward AR

INSTITUCIÓN / INSTITUTION:  - Barbara Davis Center for Childhood Diabetes, Department of Pediatrics, University of Colorado Health Sciences Center, Box B140, 4200 East 9th Ave., Denver, CO 80262, USA.

RESUMEN / SUMMARY:  - In this article, the authors covered a number of issues that affect how researchers approach prevention of diabetes. The focus has been the use of cytokines and immunosuppressive therapies. The historical understanding of cytokine and immunosuppressive approaches, new developments in using these agents in humans, and the issues involved in designing diabetes prevention trials were reviewed. Although progress at times appears slow, the current research activities predict new developments in the next few years that may improve the understanding of the progression of diabetes and possible ways to intervene.  N. Ref:: 79

 

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[188]

TÍTULO / TITLE:  - Maintenance immunosuppression in the renal transplant recipient: an overview.

REVISTA / JOURNAL:  - Am J Kidney Dis 2001 Dec;38(6 Suppl 6):S25-35.

AUTORES / AUTHORS:  - Gaston RS

INSTITUCIÓN / INSTITUTION:  - Division of Nephrology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. rgaston@nrtc.uab.edu

RESUMEN / SUMMARY:  - Managing maintenance immunosuppressive regimens after kidney transplantation is often challenging and confusing, requiring careful attention to efficacy, dosing, adverse effects, and costs of multiple medications. Most protocols combine a primary immunosuppressant (cyclosporine or tacrolimus) with one or two adjunctive agents (azathioprine, mycophenolate mofetil, sirolimus, corticosteroids). Avoiding drug-drug interactions is a major part of effective immunosuppressant management, and special situations (eg, pregnancy, intravenous dosing, caring for minority patients) can prove especially daunting. This review summarizes available data regarding current practices in maintenance immunosuppression, emphasizing issues that arise in day-to-day management of renal transplant recipients.  N. Ref:: 69

 

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[189]

TÍTULO / TITLE:  - Regulation of endothelial-type NO synthase expression in pathophysiology and in response to drugs.

REVISTA / JOURNAL:  - Nitric Oxide 2002 Nov;7(3):149-64.

AUTORES / AUTHORS:  - Li H; Wallerath T; Munzel T; Forstermann U

INSTITUCIÓN / INSTITUTION:  - Department of Pharmacology, Johannes Gutenberg University, Obere Zahlbacher Strasse 67, D-55101, Mainz, Germany.

RESUMEN / SUMMARY:  - In many types of cardiovascular pathophysiology such as hypercholesterolemia and atherosclerosis, diabetes, cigarette smoking, or hypertension (with its sequelae stroke and heart failure) the expression of endothelial NO synthase (eNOS) is altered. Both up- and downregulation of eNOS have been observed, depending on the underlying disease. When eNOS is upregulated, the upregulation is often futile and goes along with a reduction in bioactive NO. This is due to an increased production of superoxide generated by NAD(P)H oxidase and by an uncoupled eNOS. A number of drugs with favorable effects on cardiovascular disease upregulate eNOS expression. The resulting increase in vascular NO production may contribute to their beneficial effects. These compounds include statins, angiotensin-converting enzyme inhibitors, AT1 receptor antagonists, calcium channel blockers, and some antioxidants. Other drugs such as glucocorticoids, whose administration is associated with cardiovascular side effects, downregulate eNOS expression. Stills others such as the immunosuppressants cyclosporine A and FK506/tacrolimus or erythropoietin have inconsistent effects on eNOS. Thus regulation of eNOS expression and activity contributes to the overall action of several classes of drugs, and the development of compounds that specifically upregulate this protective enzyme appears as a desirable target for drug development.  N. Ref:: 201

 

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[190]

TÍTULO / TITLE:  - Novel pharmacotherapeutic approaches to prevention and treatment of GVHD.

REVISTA / JOURNAL:  - Drugs 2002;62(6):879-89.

AUTORES / AUTHORS:  - Jacobsohn DA; Vogelsang GB

INSTITUCIÓN / INSTITUTION:  - Oncology and Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. jacobda@jhmi.edu

RESUMEN / SUMMARY:  - Acute and chronic graft versus host disease (GVHD) remain the major barriers to successful hematopoietic cell transplantation. The induction of GVHD may be divided into three phases: recipient conditioning;donor T-cell activation; andeffector cells mediating GVHD. This review examines GVHD prevention and treatment using this conceptual model as framework. The various pharmacological agents discussed impact on different phases of the GVHD cascade. For example, keratinocyte growth factor and interleukin (IL)-11 are cytokines that may be useful in disrupting phase I of the GVHD cascade by blocking gastrointestinal tract damage, and lowering serum levels of lipopolysaccharide and tumour necrosis factor (TNF)-alpha. Cyclosporin, tacrolimus (FK-506) and sirolimus (rapamycin) are some of the main agents that disrupt phase II (donor T-cell activation). Mycophenolate mofetil and tresperimus probably act on this phase as well. Other novel drugs that affect phase II are tolerance-induction agents such as CTLA-4 and anti-CD40-ligand monoclonal antibodies, and preliminary results using CTLA-4 monoclonal antibody in GVHD prevention are encouraging. Examples of agents that disrupt phase III are the IL-2 receptor antagonist daclizumab and the anti-TNFalpha monoclonal antibody infliximab. These anti-cytokine antibodies have shown promising results in early studies. The most effective approach to GVHD prevention will probably be a combination regimen where the three phases of the GVHD cascade are disrupted. Once GVHD has occurred, all three phases of the cascade are activated. Developments of combination therapy for treatment of both acute and chronic GVHD are likely to yield better results than monotherapy. The numerous new treatment modalities presented should improve the outlook for patients with acute and chronic GVHD.  N. Ref:: 74

 

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[191]

TÍTULO / TITLE:  - What’s in the pipeline? New immunosuppressive drugs in transplantation.

REVISTA / JOURNAL:  - Am J Transplant 2002 Nov;2(10):898-903.

AUTORES / AUTHORS:  - Vincenti F

INSTITUCIÓN / INSTITUTION:  - University of California, San Francisco, Kidney Transplant Service, 505 Parnassus Avenue, Room 884M, San Francisco, CA 94143-0116, USA. vincentif@surgery.ucsf.edu

RESUMEN / SUMMARY:  - In the pipeline, there are a number of novel immunosuppressive drugs in preclinical development or in early clinical trials. The major target of new agents are cell-surface molecules important in immune cell interactions (especially the costimulatory pathway), signaling pathways that activate T cells, T-cell proliferation and trafficking and recruitment of immune cells responsible for rejection. The most promising biologic agents include a humanized anti-CD11a (anti-LFA1), humanized anti-B7.1/B7.2, a second-generation CTLA4Ig (LEA29Y) and a humanized antibody to anti-CD45 RB. Inhibitors of T-cell activation and signaling are still in preclinical development. The most interesting inhibitors of T-cell proliferation include inhibitors of the Janus protein tyrosine kinase, JAK3, and FK778, a leflunomide analog. Chemokines play an important role in rejection by virtue of their critical role as regulator of trafficking and activation of lymphocytes. Early trials of FTY720, a synthetic small molecule with functional homology to sphingosine-1 phosphate leading to lymphocyte sequestration, appear very promising; however, enthusiasm for this drug is mitigated by its potential cardiac side-effects. Antagonists to several chemokine receptors, including CCR1, CXCR3 and CCR5, have been shown to be effective in experimental transplantation and are likely to be considered for clinical development.  N. Ref:: 46

 

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[192]

TÍTULO / TITLE:  - Current and potential agents for the treatment of alopecia areata.

REVISTA / JOURNAL:  - Curr Pharm Des 2001 Feb;7(3):213-30.

AUTORES / AUTHORS:  - Freyschmidt-Paul P; Hoffmann R; Levine E; Sundberg JP; Happle R; McElwee KJ

INSTITUCIÓN / INSTITUTION:  - Department of Dermatology, Philipp University, Marburg, Germany. freyschm@mailer.uni-marburg.de

RESUMEN / SUMMARY:  - Alopecia areata is considered to be a T-cell mediated autoimmune disease of the hair follicle. Current immunosuppressive approaches and immunomodulatory treatment with contact sensitizers such as diphenylcyclopropenone and squaric acid dibutylester are dealt with in this review article. The efficacy of the various modes of treatment is evaluated by a review of literature and their mode of action is discussed. In accordance with the mechanism of autoimmune pathogenesis of AA, improved future treatments may be immunosuppressive or immunomodulatory, or they should otherwise protect the hair follicle from the injurious effects of the inflammation. Such possible future therapeutic approaches include the use of liposomes as an improved vehicle, application of immunosuppressive cytokines like TGF-beta and IL-10, inhibition of apoptosis mediated by the Fas-FasL system, inhibition of the lymphocyte homing receptor CD44v10, induction of tolerance as well as principles of gene therapy.  N. Ref:: 141

 

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[193]

TÍTULO / TITLE:  - Oral lichen planus: an update.

REVISTA / JOURNAL:  - Drugs Today (Barc) 2002 Aug;38(8):533-47.

AUTORES / AUTHORS:  - Agarwal R; Saraswat A

INSTITUCIÓN / INSTITUTION:  - Pharmaceutics Research Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India. ravindra_angarwal1@yahoo.com

RESUMEN / SUMMARY:  - Oral lichen planus (OLP) is a chronic autoimmune disease of unknown etiology that affects the skin and mucosae, including the oral cavity. The disease is characterized by lacy, thin white lines on a violaceous background on the oral mucosa, usually on the inside of the cheeks. The disease is also characterized by chronic inflammation and is often associated with severe pain and a burning sensation in the mouth. The etiopathogenesis of lichen planus is complex, with the involvement of T lymphocytes, mast cells, intercellular adhesion molecule-1 (ICAM-1) and major histocompatibility complex class II antigens. The immunologic process results in vacuolar degeneration, lysis of basal cells and, ultimately, liquefaction of the basal cells. The precipitating factors of OLP can be: stress, particular foods, dental plaque, systemic illness and poor oral hygiene. Often no medication is necessary for benign disease. In the case of severe pain and the burning sensation, high potency topical corticosteroids remain the most reliably effective treatment. Other available treatments are topical cyclosporine, tacrolimus, retinoids and other immunosuppressive agents. Systemic corticosteroids may be indicated in patients whose condition is unresponsive to topical corticosteroids. However, OLP remains a challenging disease to treat, in spite of the numerous treatments tried in the patient population.  N. Ref:: 120

 

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[194]

TÍTULO / TITLE:  - Early clinical experience with a novel rapamycin derivative.

REVISTA / JOURNAL:  - Ther Drug Monit 2002 Feb;24(1):53-8.

AUTORES / AUTHORS:  - Nashan B

INSTITUCIÓN / INSTITUTION:  - Klinik fur Viszeral-und Transplantationschirurgie, Medizinische Hochschule Hannover, Hannover, Germany. nashan@tx-amb.mh-hannover.de

RESUMEN / SUMMARY:  - SDZ RAD (everolimus, Certican is a novel macrolide immunosuppressant that blocks growth factor-driven transduction signals in the T-cell response to alloantigen. After stimulation of the IL-2 receptor on the activated T-cell, SDZ RAD inhibits p70S6 kinase, acting at a later stage in the T-cell mediated response than do cyclosporine and other calcineurin inhibitors (CNIs). Unlike the CNIs, SDZ RAD is a proliferation signal inhibitor, blocking growth factor-driven proliferation of both hematopoietic and nonhematopoietic cells. These activities are complementary to those of cyclosporine and provide a rationale for the addition of SDZ RAD to cyclosporine-based immunosuppression, with the potential for minimizing CNI nephrotoxicity, reducing the incidence of acute rejection, and favoring long-term graft survival. Potential also exists for beneficial effects on other factors that may influence the development of chronic rejection. These factors include comorbid diseases such as hypertension, which may affect transplant vasculopathy, and opportunistic infection with cytomegalovirus (CMV) and other viruses, which may increase the risk of chronic rejection. The synergistic effect of SDZ RAD and cyclosporine has been confirmed in preclinical models, with graft survival being significantly prolonged in rat models of kidney and heart allotransplantation. Clinical experience with SDZ RAD in cyclosporine-based immunosuppression, including low-dose cyclosporine regimens, has also resulted in predictable and favorable clinical outcomes. Low rates of acute rejection, excellent rates of patient and graft survival, lower incidence of CMV infections, better cholesterol, triglyceride and creatinine profiles, and better renal function have been demonstrated with SDZ RAD and lower doses of cyclosporine (Neoral; Novartis) in recipients of renal transplants. These findings, combined with good tolerability rates and an acceptable side-effect profile, indicate that the synergistic profile of SDZ RAD in combination with nontoxic dosages of CNI’s and IL2 inhibitors will further improve longterm results in renal transplantation.  N. Ref:: 48

 

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[195]

TÍTULO / TITLE:  - Tolerance to islet autoantigens in type 1 diabetes.

REVISTA / JOURNAL:  - Annu Rev Immunol 2001;19:131-61.

      ●● Enlace al texto completo (gratuito o de pago) 1146/annurev.immunol.19.1.131

AUTORES / AUTHORS:  - Bach JF; Chatenoud L

INSTITUCIÓN / INSTITUTION:  - INSERM U 25, Hopital Necker, 161 rue de Sevres, Paris Cedex 15, 75743 France. bach@necker.fr

RESUMEN / SUMMARY:  - Tolerance to beta cell autoantigens represents a fragile equilibrium. Autoreactive T cells specific to these autoantigens are present in most normal individuals but are kept under control by a number of peripheral tolerance mechanisms, among which CD4(+) CD25(+) CD62L(+) T cell-mediated regulation probably plays a central role. The equilibrium may be disrupted by inappropriate activation of autoantigen-specific T cells, notably following to local inflammation that enhances the expression of the various molecules contributing to antigen recognition by T cells. Even when T cell activation finally overrides regulation, stimulation of regulatory cells by CD3 antibodies may reset the control of autoimmunity. Other procedures may also lead to disease prevention. These procedures are essentially focused on Th2 cytokines, whether used systemically or produced by Th2 cells after specific stimulation by autoantigens. Protection can also be obtained by NK T cell stimulation. Administration of beta cell antigens or CD3 antibodies is now being tested in clinical trials in prediabetics and/or recently diagnosed diabetes.  N. Ref:: 153

 

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[196]

TÍTULO / TITLE:  - Early experience using calcineurin-free protocol in recipients of high-risk cadaver renal transplants.

REVISTA / JOURNAL:  - Transplant Proc 2002 Aug;34(5):1627-8.

AUTORES / AUTHORS:  - El-Sabrout R; Delaney V; Butt F; Qadir M; Rashid I; Hanson P; Butt K

INSTITUCIÓN / INSTITUTION:  - Departments of Transplantation/Vascular Surgery, New York Medical College, Valhalla, New York, USA.

 

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[197]

TÍTULO / TITLE:  - Reactivation of replication of hepatitis B and C viruses after immunosuppressive therapy: an unresolved issue.

REVISTA / JOURNAL:  - Lancet Oncol 2002 Jun;3(6):333-40.

AUTORES / AUTHORS:  - Vento S; Cainelli F; Longhi MS

INSTITUCIÓN / INSTITUTION:  - Section of Infectious Diseases, Department of Pathology, University of Verona, Borgo Trento Hospital, Verona, Italy. ventosandro@yahoo.it

RESUMEN / SUMMARY:  - The liver is susceptible to the toxic effects of many cytotoxic or immunosuppressive treatments. However, in carriers of hepatitis B virus (HBV) and, less frequently, of hepatitis C virus, liver damage due to reactivation of viral replication can occur after withdrawal of immunosuppressive drugs. These reactivations, which are associated with fulminant forms of hepatitis in up to 25% of cases, are observed both in symptom-free chronic carriers of hepatitis B surface antigen and in patients who have chronic hepatitis B or C and concurrent haematological tumours or solid neoplasms or who have received transplants. HBV-related complications may cause delays or modifications of therapy, and the chance of cure is reduced. In this review, we analyse clinical, biochemical, and serological issues in reactivation of viral replication and examine the role of immune reactions in the pathogenesis and the possible toxicity of immunosuppressive drugs. We emphasise the importance of identifying predictive markers of a clinically relevant reactivation, review difficulties in drug prevention and treatment, indicate studies that are needed to address the key clinical issues, and give practical recommendations to practising physicians and oncologists.  N. Ref:: 60

 

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[198]

TÍTULO / TITLE:  - Hepatitis B virus (HBV) reactivation after cytotoxic or immunosuppressive therapy—pathogenesis and management.

REVISTA / JOURNAL:  - Rev Med Virol 2001 Sep-Oct;11(5):287-99.

      ●● Enlace al texto completo (gratuito o de pago) 1002/rmv.322 [pii]

AUTORES / AUTHORS:  - Xunrong L; Yan AW; Liang R; Lau GK

INSTITUCIÓN / INSTITUTION:  - University Department of Medicine, Queen Mary Hospital, 102 Pokfulum Road, Hong Kong SAR, China.

RESUMEN / SUMMARY:  - In an endemic area for chronic hepatitis B infection, reactivation of this virus is a serious cause of morbidity and mortality in patients undergoing cytotoxic or immunosuppressive therapy. Careful prospective serological testing has shown that hepatitis B virus reactivation is a two-staged process. The initial stage occurs during intense cytotoxic or immunosuppressive therapy and is characterised by enhanced viral replication, as reflected by increases in the serum levels of hepatitis B virus DNA, hepatitis B e antigen, hepatitis B virus DNA polymerase and infection of naive hepatocytes with hepatitis B virus. The second stage is related to restoration of immune function following withdrawal of cytotoxic or immunosuppressive therapy, which causes rapid immune-mediated destruction of infected hepatocytes. Clinically, this can lead to hepatitis, hepatic failure and even death. The occurrence and severity of hepatitis B virus reactivation after various cytotoxic or immunosuppressive therapy is unpredictable and treatment has been disappointing, largely due to the late administration of therapy. Recently, pre-emptive treatment of chronic hepatitis B patients undergoing cytotoxic or immunosuppressive therapy, with potent nucleoside analogues has shown some promising results. Further controlled studies are needed to define the incidence and risk factors of hepatitis B reactivation so that pre-emptive treatment with nucleoside analogues could be administered to those patients at high risk of disease.  N. Ref:: 93

 

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[199]

TÍTULO / TITLE:  - Osteoporosis after liver transplantation.

REVISTA / JOURNAL:  - Liver Transpl 2003 Apr;9(4):321-30.

      ●● Enlace al texto completo (gratuito o de pago) 1053/jlts.2003.50044

AUTORES / AUTHORS:  - Compston JE

INSTITUCIÓN / INSTITUTION:  - Department of Medicine, University of Cambridge School of Clinical Medicine, United Kingdom. jec1001@cam.ac.uk

RESUMEN / SUMMARY:  - Osteoporosis remains a serious potential complication of liver transplantation, although its incidence may be significantly reduced by the use of lower doses of glucocorticoids. Additional factors likely to contribute to its pathogenesis include other immunosuppressive agents, particularly cyclosporin A and FK506, vitamin D insufficiency, secondary hyperparathyroidism, hypogonadism and pre-existing bone disease. Bone density assessment and spinal X-rays should be performed before transplantation to assess subsequent fracture risk and vitamin D and gonadal status assessed. Measures should be taken to optimise bone health prior to transplantation; in those with low bone mineral density and/or previous fragility fracture, prophylaxis against bone loss after transplantation should be considered. Although anti-fracture efficacy has not been established for any agent there is evidence, mainly in patients undergoing other forms of solid organ transplantation, that repeated infusions of pamidronate may be effective in preventing bone loss.  N. Ref:: 81

 

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[200]

TÍTULO / TITLE:  - Management of internal fistulas in Crohn’s disease.

REVISTA / JOURNAL:  - Inflamm Bowel Dis 2002 Mar;8(2):106-11.

AUTORES / AUTHORS:  - Levy C; Tremaine WJ

INSTITUCIÓN / INSTITUTION:  - IBD Clinic, Division of Gastroenterology and Hepatology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, U.S.A.

RESUMEN / SUMMARY:  - Internal fistulas occur in 5-10% of patients with Crohn’s disease. The clinical presentation of each of the three main types of internal fistulas—enteroenteric, enterovaginal, and enterovesical fistulas—is important in determining the best management. Asymptomatic fistulas usually require no treatment, but fistulas that cause severe or persistent symptoms necessitate intervention. Previously regarded as a surgical condition requiring resection, some internal fistulas are amenable to a more conservative approach involving medical therapy, surgical repair, or both. So far, there have not been any prospective studies designed specifically to assess the efficacy of a medical treatment of internal fistulas, and information about treatment results is gleaned from trials in which patients with internal fistulas have been included and from retrospective reports. Drugs that have been reported to close internal fistulas partially or completely include azathioprine, 6-mercaptopurine, mycophenolate mofetil, cyclosporine A, tacrolimus, and infliximab. Reparative surgical techniques include transrectal and transvaginal mucosal advancement flaps, cutaneous advancement flap, and anal stricturectomy in combination with a rectal mucosal advancement sleeve. Prospective trials of medical therapy and combination medical and surgical therapy for internal fistulas are needed to provide evidence to support the use of these new therapeutic approaches.  N. Ref:: 44

 

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